• Asian-Australasian Journal of Animal Sciences
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• 제24권2호
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• pp.250-257
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• 2011

An experiment was conducted to determine the effects of different mycotoxin adsorbents including esterified glucomannan (EGM), hydrated sodium calcium aluminosilicate (HSCAS) and compound mycotoxin adsorbent (CMA) on performance, blood parameters, and liver pathological changes in broilers fed mold-contaminated feed. Two hundred and forty 10-day-old broilers were randomly assigned to one of the five dietary treatments including: i) control diet; ii) mold-contaminated diet; iii) moldcontaminated diet+0.05% EGM; iv) mold-contaminated diet+0.2% HSCAS; v) mold-contaminated diet+0.1% CMA. At 35-days-old, blood and liver tissue samples were collected for analysis. 0.1% CMA improved ADG and ADFI during 10-42 d compared to the moldcontaminated group (p<0.05). The mold-contaminated diet increased total white blood cell (WBC) number, haemoglobin (Hgb) concentration, hematocrit (Hct) level, serum aspartate aminotransferase (AST) and ${\gamma}$-glutamyl transferase (GGT) activities, and decreased red blood cell (RBC) number and serum globulin (GLB) and urea nitrogen (BUN) concentrations (p<0.05). The three mycotoxin adsorbents alleviated the alteration of RBC, WBC, Hgb and AST caused by the mold-contaminated diet. Furthermore, 0.1% CMA increased GLB concentration and decreased Hct level and GGT activity (p<0.05). Liver superoxide dismutase (SOD) activity was reduced, and myeloperoxidase (MPO) activity was increased by the mold-contaminated diet (p<0.05). Both EGM and HSCAS prevented the increase of MPO activity (p<0.05). Liver lesion, including severe vacuolar degeneration of hepatocytes, was observed in chicks fed the mold-contaminated diet. 0.05% EGM prevented these effects except for biliary hyperplasia and mild vacuolar degeneration. 0.2% HSCAS showed medium vacuolar degeneration of hepatocytes. Liver of broilers fed 0.1% CMA revealed a mild vacuolar degeneration. These results indicate that a mold-contaminated diet results in adverse effects on blood parameters and liver morphology. 0.05% EGM and 0.2% HSCAS partially alleviated the adverse effects. However, 0.1% CMA almost completely ameliorated the adverse effects.

• Asian-Australasian Journal of Animal Sciences
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• 제25권4호
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• pp.569-576
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• 2012

Two experiments were conducted to investigate the potential for Forsythia suspensa extract (FSE) to substitute for antibiotic in broiler chicken. First, a well-diffusion assay procedure and a 2-fold dilution method were used to determine the bacteriostatic activity of FSE on Escherichia coli K88, staphylococcus aureus, and salmonella was assayed. An inhibitory effect of FSE was observed on the growth of these bacteria. This effect seems to be dose depended, which disappeared after 25.00, 12.50, 1.56 mg/ml. Second, a 42-d trial with 252 broiler chickens (d 1, $38.7{\pm}1.1$ g BW) was conducted to evaluate the effect of dietary supplementation of FSE in broiler chicken. The feeding program consisted of a starter diet from d 1 to 21 and a finisher diet from d 22 to 42. Dietary treatments included were: i) NC: negative control fed a corn-soybean meal based diet; ii) PC: positive control group fed based diet with chlortetracycline; and iii) FC: a test group fed with 100 mg FSE/kg diet. In this study growth performance did not differ among treatments during the starter period. However, dietary supplemental chlortetracycline and FSE increased (p<0.05) average daily gain (ADG), average daily feed intake (ADFI) compared with NC during the finisher and overall phase. Apparent digestibility of calcium on d 21, digestibility of energy and calcium on d 42 of FC was greater (p<0.05) than NC. Moreover, cecal Escherichia coli counts for birds from FC were lower (p<0.05) than NC. Dietary FSE supplementation also improved (p<0.05) villus height and villus height to crypt depth ratios in both duodenum and ileum and decreased (p<0.05) crypt depth in the duodenum. Duodenum villus height and villus height to crypt depth ratio in both duodenum and ileum from the FC group were also greater (p<0.05). Serum growth hormone and IGF-1 were not influenced by different treatments. Apparently, FSE has the potential to substitute for antibiotic in broiler chicken.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1138-1143
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• 2001

A total of 96 piglets were weaned at 19 and 13 days in Exp. 1 and 2, respectively, and allotted to one of four diets: three with different spray dried plasmas (SPs) and one with hydrolysed casein (HC). SPs were from pigs (SPP), mixed origin (SMP), and mixed origin with standardized level of immunoglobulins (SMPIG). All the diets contained 1.7% total lysine, 25% of the test protein source, 45% corn starch, 15% lactose, 2% sucrose, 7% soybean oil. At d 4 and d 2 in Exp. 1 and 2, respectively, piglets were perorally challenged with $10^{10}$ CFU E. coli K88. Growth performance, immunity, and health condition were measured for 15 days and 14 days in Exp. 1 and 2, respectively. To investigate apparent ileal digestibility and nutrient deposition, all piglets were sacrificed at d 14 in Exp. 2. In 1. 3 piglets died in HC diet and 1 in SPP diet. HC diet showed higher mortality (p<0.01) than other diets. In Exp. 2, no clinical sign of infection was detected, no difference for the content of E. coli K88 was found in feces at 4 and 6 days after the infection, and no E. coli K88 was found in the jejunum at the end of experiment. In both experiments, feed intake was lower for HC diet and ADG was 96, 106, 122 and 155 for HC, SPP, SMP and SMPIG diet, respectively (HC vs others, p<0.05; SMPIG vs other SP, p<0.01). Heal apparent digestibility of nitrogen in sacrificed piglets was higher for HC diet (p<0.05). After the challenge, K88-specific titers in saliva (Exp. 1) and in plasma (Exp. 2) were reduced in SMP and SMPIG. The piglets positive to the adhesion of the used E. coli strain to the intestinal brush borders had a significantly reduced growth (p<0.01) and a higher K88-specific IgA titer in plasma, in comparison with negative ones. This effect was independent of the diet. The data show the relevance of spray dried plasma sources and particularly of SP with standardized level of immunoglobulins for the feeding of early-weaned at the risk of infection by enterotoxigenic bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.861-867
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• 2008

The objective of this study was to evaluate the effect of exogenous ghrelin on the behaviors, weight gain, and feed intakes of weanling piglets. A total of 25 pairs of Duroc$\times$Landrace$\times$Large White piglets weaned at 21 days of age were used in this experiment which finished on day 36. Each pair of healthy piglets from the same litter with similar body weight and of the same gender were selected and randomly arranged to ghrelin or control groups. Thus, there were 50 piglets (ghrelin 25; control 25) kept in 10 pens (ghrelin 5; control 5) and 5 piglets per pen. Initial body weight of the pigs did not differ between the control and ghrelin treatment ($7.43{\pm}0.17kg$; p = 0.81). Experimental pigs were infused with ghrelin ($1{\mu}g/d$ pig) via the marginal ear vein between 0750 and 0800 h at 22, 23, 24 days of age. Control pigs were infused with 0.9% saline. Feed consumption was measured on days 23, 24, 25, 29 and 36. Body weight was measured on days 22, 23, 24, 25, 29 and 36. Behavior data of individual piglets were collected by real-time observation from 0800-1500 h through remote supervisory equipment at 22, 23 and 24 days of age. The results indicated that ghrelin infusion could increase drinking (p<0.05) and lying behaviors (p<0.01) and decrease mounting behaviors (p<0.05). No significant influence of ghrelin was found on average daily weight gain (ADG) and average daily feed intake (ADFI) in this experiment (p>0.05). In conclusion, exogenous ghrelin by the method above and at the dosage of $1{\mu}g/d$ pig could cause a variety of behavioral effects, but not improve performance of weanling piglets.

• Asian-Australasian Journal of Animal Sciences
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• 제24권3호
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• pp.351-357
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• 2011

An experiment was conducted to evaluate the effects of castration on growth performance, carcass characteristics, and meat quality of goat kids. Fourteen Boer-cross buck and wether goat kids (n = 7; initial body weight (BW) $38.0{\pm}0.35\;kg$ and $34.8{\pm}0.35\;kg$, for bucks and wethers, respectively) were grazed on annual Marshall ryegrass (Lolium multiflorum Lam.) for 56 days. Body weights were recorded after 4 h withdrawal from feed and water for two consecutive days, every 2 wk. After d 56, animals were harvested and hot carcass weight (HCW), cold carcass weight (CCW), dressing percent (DP), kidney and pelvic fat (KPF), longissimus muscle (LM) area, back fat (BF), and other carcass parameters were measured. Day 0 BW was used as a covariate for analyses. However, bucks were heavier than wethers at d 15 (p = 0.09), 42 (p = 0.001) and 56 (p = 0.001). Bucks had higher ADG (146 vs. 74 g/d; p = 0.001), HCW (21.2 vs. 18.8 kg; p = 0.06) and CCW (20.3 vs. 17.9 kg; p = 0.04) when compared with wether goats. Dressing percentage (51 vs. 47%; p = 0.06), KPF (0.44 vs. 0.16%; p = 0.02) and BF (0.41 vs. 0.21 cm; p = 0.05) were higher in wethers vs bucks, respectively; however, USDA live or carcass grades were similar. Longissimus muscle tissue from wethers and bucks were similar in darkness ($L^*$) and redness ($a^*$), but wethers had more (p = 0.02) yellow tint ($b^*$). Palmitic (C16:0), stearic (C18:0) and oleic (C18:1) acids were higher (p = 0.001) in muscle tissue from wethers compared to bucks. The saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) contents of muscle tissue were lower (p = 0.001) for bucks with no difference in polyunsaturated fatty acids (PUFA). Longissimus muscle initial temperature was higher in bucks (p<0.04) and pH change post-mortem was similar for bucks and wethers. These results indicated that castration of young market goats reduced growth performance and produced carcasses with more fat and higher SFA.

• Asian-Australasian Journal of Animal Sciences
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• 제15권6호
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• pp.872-877
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• 2002

Two experiments were conducted to determine the effects of reducing particle size of corn from 1,000 to 500 ${\mu}m$ in simple and complex diets for nursery pigs and broiler chicks. In Exp 1., 192 nursery pigs were used in a 24 d growth assay. Treatments were: 1) 1,000 ${\mu}m$ corn in a simple diet; 2) 500 ${\mu}m$ corn in a simple diet; 3) 1,000 ${\mu}m$ corn in a complex diet; and 4) 500 ${\mu}m$ corn in a complex diet. Overall, pigs fed complex diets had 9% greater ADG (p<0.005) and 5% greater gain/feed (p<0.01) compared to pigs fed simple diets. Also, pigs fed the 500 ${\mu}m$ treatments had 3% better overall gain/feed than those fed the 1,000 ${\mu}m$ treatments (p<0.007). At d 9, apparent digestibilities of DM, N and GE were greater for complex diets and diets with smaller particle size (p<0.02). At d 23, there were no differences in nutrient digestibility resulting from diet complexity, but pigs fed diets with corn ground to 500 ${\mu}m$ had greater digestibility of DM (p<0.02) and GE (p<0.003) than pigs fed diets with corn ground to 1,000 ${\mu}m$. A second experiment was designed to determine if four days old broiler chicks were an acceptable model for predicting the effects of feed processing procedures on nursery pigs. Chicks fed complex diets had 3% greater gain/feed than chicks fed simple diets (p<0.001). Rate of gain and feed intake were improved by 3 and 2%, respectively, for chicks fed crumbled diets (p<0.03). However, there were several significant interactions among the main effects. For instance, crumbling did not affect gain/feed in chicks fed complex diets, but rate of gain and feed intake were increased by 7 and 6%, respectively, when simple diets were crumbled (diet complexity${\times}$diet form, p<0.001). Also, gain/feed of chicks was improved by 3% when particle size was reduced in meal diets, but not affected in chicks fed crumbles (diet form${\times}$particle size, p<0.005). Thus, our data suggested that reduction of particle size of corn was important for simple and complex diets and that a complex diet with 1,000 ${\mu}m$ corn gave no better performance than a simple diet with 500 ${\mu}m$ corn.

• Asian-Australasian Journal of Animal Sciences
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• 제15권6호
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• pp.838-843
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• 2002

The objectives of this study were to examine growth performance and meat quality by three different feeding patterns in Korean native cattle (KNC). In each of 3 years, fifteen KNC were randomly assigned in a (3 feeding management)${\times}$(3 sex) factorial design experiment; thus, in total, there were 5 animals in each of the 9 treatments. The three feeding management treatments were longterm (24 month) restriction feeding (LTFR), long-term restriction feeding-hormone implant (LTFR-tH), and short-term (18 month) nonrestriction feeding (STFNR). Three sexes were bull, steer, and heifer. Concentrate diet was fed restriction-feeding method based on body weight in LTFR and LTFR-tH. However, the diet was fed ad libitum in STFNR. Hormonal implantation was made three times with M-$PO^{TM}$ for bulls and with F-$TO^{TM}$ for heifers at 18, 20, 22 month of age in LTFR-tH. Animal were purchased from the local cattle market and managed in two local farms and at the university research unit. Animals were slaughtered at 24 months for long-term trial and at 18 month for short-term trial. The growth rate was the highest in bulls and the lowest in heifers. However, the differences were diminished in F-$TO^{TM}$ implanted heifers. The average daily gain was high in STFNR due to ad libitum feeding. The carcass grade was similar among the treatments on percentage bases. Hormonal implants improved significantly the meat quality grade in all sexes. Castration increased body fat content and improved meat quality grade by intramuscular fat deposition. In conclusion, long-term feeding and hormone treatment increased meat quality grade more than short-term feeding. However, ADG was higher in the short-term trial although feedefficiency was lower.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1534-1541
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• 2001

Forty-five Large White gilts were used to study the effect of energy intake from 28 to 176 d of age on body composition and reproductive development. From 28 to 60 d, the gilts were fed ad libitum a 16.6 MJ DE/kg, 24% crude protein and 1.3% total lysine diet. From 61 d of age three dietary treatments were used; 1) ad libitum access to feed (15.6 MJ DE/kg, 21% crude protein and 1.07% total lysine) (H), 2) feed offered at 75% (M) of the previous days intake of H, and 3) feed offered at 60% (L) of the previous days intake of H. ADG from 61 to 176 d of age was (p<0.05) affected by treatment. Although live weight at 176 d of age did not differ (p>0.1) the H gilts had higher (p<0.08) carcass weights than the M or L gilts. Back fat depths were similar (p>0.1) for all treatments at 115 d of age, however by 176 d of age M and H gilts were fatter (p<0.1) than L gilts. The mean lipid deposition (LD) from 115 to 176 d of age for L gilts (78.9 g/d) was less (p<0.05) than for M gilts (143.6 g/d) and H gilts (135.6 g/d). There were no differences between treatments for protein deposition (PD) over the same period. More (p<0.05) H gilts (n=8) attained puberty (first observed estrus) than either M gilts or L gilts (n=4 for both). Follicle numbers were similar (p>0.1) across treatments. For gilts that attained puberty, H gilts had fewer (p<0.05) follicles (13.5) than M gilts (19.7) and L gilts (21.3). For gilts with follicular development, H gilts had the heaviest (458.7 g) reproductive tract weight (RTW). However, for those that attained puberty, L gilts had the heaviest RTW. RTW were lowest for those with no follicular development. Energy restriction had a negative impact on puberty attainment, i.e. it took longer to reach puberty. However, for gilts that attained puberty, the number of follicles was greater for those on r feed intakes. It would appear that rate of fat deposition, but not necessarily the total amount of fat, plays an important role in puberty attainment.

• Asian-Australasian Journal of Animal Sciences
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• 제19권1호
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• pp.72-79
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• 2006

1,225 healthy day-old avian broiler chicks were used to investigate the effects of activated charcoal (AC, made from willow tree), hydrated sodium calcium aluminosilicates (HSCAS) and esterified glucomannan (EGM) supplementation on broiler performance, blood profiles and meat characteristics when less moldy or moldy corn was included to formulate seven isocaloric and isonitrogenous diets: Positive Control (less moldy corn diet, PC), PC+2% AC, Negative Control (moldy corn, NC), NC+0.05% EGM, NC+0.1% EGM, NC+0.5% HSCAS and NC+1% AC. PC+2% AC resulted in lower growth rate, poorer feed conversion ratio (FCR), more leg problems and higher mortality of birds than those fed PC diet (p<0.05). Inclusion of 0.05% EGM, 0.1% EGM, 0.5% HSCAS and 1% AC in NC diet did not improve average daily weight gain (ADG) or affect feed intake of birds during the first or the second three-week periods. However, 0.05% EGM tended to (p>0.05) and 0.1% EGM significantly (p<0.05) improved FCR during the first three-week period. Breast meat of NC birds had higher Minolta $L^*$ values (white) but lower $a^*$ (reddish) and $b^*$ (yellowish) values (p<0.01) than the PC birds. Addition of 0.05% EGM and 0.1% EGM in NC diet reduced the $L^*$ values (p<0.05), improved $a^*$ and $b^*$ values (p<0.05) of breast meat of birds fed NC diet, but had no effect on meat color when 0.5% HSCAS or 1% AC was included (p>0.05). Relative weight of liver to body was reduced by feeding NC diet (p<0.05) and could not be normalized by different mycotoxin adsorbents (p>0.05) to the ratio of the PC birds. Relative weight of cholecyst of NC birds was increased compared with PC birds and could only be normalized by addition of 0.05% EGM and 1% AC (p<0.05) in NC diet. NC birds had lower serum albumin level than the PC birds (p<0.05) and addition of 0.05% EGM or 1% AC in NC diet did normalize serum albumin level. Addition of 0.5% HSCAS in NC diet further reduced serum albumin, globulin, total protein and uric acid levels (p<0.05). It was concluded that lower FCR during the first three-week period of growth and deterioration of meat quality observed in bird fed moldy corn with moderate T2 and fuminisin contamination and damaged nutrients and pigment availability, might be improved by dietary supplementation of 0.05% to 0.1% EGM, but not by 1% AC or 0.5% HSCAS supplementation.

• Asian-Australasian Journal of Animal Sciences
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• 제23권5호
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• pp.573-579
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• 2010

This study was conducted to determine the effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance, nutrient digestibility, gas emission, diarrhea incidence, and fecal copper concentration in growing pigs by using a 2${\times}$2 factorial design. In this trial, 96 pigs (63 d of age) were employed, with an average initial weight of 28.36${\pm}$1.14 kg. The dietary treatments were i) basal diet with 134 ppm copper (Korea recommendation) as $CuSO_4$+tallow; ii) basal diet with 134 ppm Cu as $CuSO_4$+glycerol; iii) basal diet with 134 ppm copper as CuMet+tallow; and iv) basal diet with 134 ppm copper as CuMet+ glycerol. Throughout the entire experimental period, no differences were noted among treatment groups with regard to the magnitude of improvement in ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain:feed) ratios. The nitrogen (N) digestibility of pigs fed on diets containing organic copper was improved as compared with that observed in pigs fed on diets containing inorganic copper (p<0.05). An interaction of copper${\times}$energy was observed in the context of both nitrogen (p<0.05) and energy (p<0.01) digestibility. Ammonia emissions were significantly lower in the organic copper-added treatment groups than in the inorganic copperadded treatment groups (p<0.05). Mercaptan and hydrogen sulfide emissions were reduced via the addition of glycerol (p<0.05). No significant effects of copper or energy source, or their interaction, were observed in reference to diarrhea appearance and incidence throughout the entirety of the experimental period. The copper concentration in the feces was significantly lower in the organic copper source treatment group than was observed in the inorganic copper source treatment group (p<0.05). The results of this experiment show that organic copper substituted for inorganic copper in the diet results in a decreased fecal copper excretion, but exerts no effect on performance. The different energy (tallow and glycerol) sources interact with different copper sources and thus influence nutrient digestibility. Glycerol supplementation may reduce the concentrations of odorous sulfuric compounds with different Cu sources.