• Journal of Sericultural and Entomological Science
• /
• v.53 no.1
• /
• pp.44-49
• /
• 2015

We characterized tissue specific-expressed genes in the posterior silk gland of Bombyx mori using by the Annealing Control Primer based differential display-PCR manner. In this study, we isolated 34 differentially expressed PCR amplicons, which one of these was identified as a novel transcript named as ACP-16 (366 bp), its expression was observed only in the posterior silk glands by Northern blot analysis. To determine promoter region of the ACP-16, we isolated and analyzed a phage DNA having 1.7 kb-long genome DNA including the open reading flame and 5'- upstream untranslated region of the ACP-16 gene from a genomic DNA library. We have estimated a promoter region of the ACP-16 gene by a web promoter prediction engine, which locates -750 ~ -165 from translation initiation site (ATG, +1). ACP-16 gene is necessary to more studies about critical biological role in order to apply the silkworm's transgenic system.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.35 no.4
• /
• pp.684-691
• /
• 2008

Many operations have been carried out using the fluoride ion to prevent and reverse dental caries. It certainly encourages remineralization and also prevents dental caries. However, the remineralization developed by these means is superficial only leaving lower levels of demineralized lesion with a degree of porosity and incomplete mineralization. We must consider its toxic effects when it is ingested for overdose. The CPP-ACP paste is able to remineralize the incipient lesion and has no harmful effects when it is ingested, because it was made form casein which is from the protein of milk. The purpose of this article is to compare the remineralization effects between APF gel and the CPP-ACP paste. we applicated the APF gel and CPP-ACP paste on the artificial enamel carious lesion. After 14 days, we measured the surface microhardness and observed the remineralized lesion under polarized light microscope. The results were as follows : 1. The surface microhardness of group III was the highest, followed by group II, and I(p<0.05). 2. The surface microhardness of group III was significantly higher than those of group I, and II(p<0.05). 3. We could observe thin and irregular remineralization layer of group II, and regular and moderate remineralization layer of group III under polirized light microscope.

• Applied Chemistry for Engineering
• /
• v.29 no.6
• /
• pp.740-745
• /
• 2018

The synthesis and characterization of amorphous calcium phosphate (ACP) nanoparticles were reported in this work. We show that relatively monodisperse ACP nanoparticles with a size of sub-100 nm can be prepared by a hydrothermal reaction of calcium chloride ($CaCl_2$) and disodium adenosine triphosphate ($Na_2ATP$) in the presence of sodium phytate as an additive. Their compositions and structures were confirmed using a series of material characterization techniques. Our results exhibit that ACP nanoparticles synthesized using sodium phytate enhanced the stability of maintaining their amorphous nature and prevented from a conversion to crystalline hydroxyapatite (HAP). ACP nanoparticles with the improved stability have potential uses in biomaterial applications in regenerative medicine.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2007.06a
• /
• pp.353-354
• /
• 2007

• Korean Journal of Plant Resources
• /
• v.2 no.2
• /
• pp.312-318
• /
• 1989

가을무우의 궁중 무우 6품종 및 연마무우 4품종에 관하여 Acp, NDH 및 IDH의 다형적변이를 조사하였다. 1. Acp에서는 7개의 Band가 (PH 7.0~8.0),NH에서는 2개의 Band가 (PH7.5~8.0) IDH에서는 3개의 Band가 (PH 5.0~6.0) 검출되었다. 2.Acp-1, -2, -3, -5 및 -6의 band, NDH-2의 Band 및 IDH-2의 Band 가 전품조에서 고정적인 Band로 검축되었다. 3.궁중군에서는 NDH-1, Acp-4 의 Band가 전혀 검출되지 않았다. 4. 다형적인 변이가 보여진 Acp-4 및 -7, NDH-1, IDH-1 및 -3의 5가지의 Band에서 품종판별로 Marker로 활용할 수 있음이 밝혀졌다.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2004.02a
• /
• pp.191-204
• /
• 2004

The research and development of effective management technologies of the spent fuels discharged from power reactors are an important and essential task of KAERI. In resent several years KAERI has focused on a project named "development and demonstration of the Advanced spent fuel Conditioning Process (ACP) in a laboratory scale." The Facility for ACP demonstration consists of two Hot Cells and auxiliary facilities. It is now in the final design stage and will be constructed in 2004. After construction of the facility the ACP equipments will be installed in Hot Cells. The ACP will be demonstrated by some simulated spent fuels first and then by spent fuels.

• Journal of Hospice and Palliative Care
• /
• v.24 no.3
• /
• pp.144-153
• /
• 2021

Purpose: Advance care planning (ACP) is widely understood to improve end-of-life care. This systematic review and meta-analysis aimed to examine the effects of ACP interventions on decision conflict and psychological distress. Methods: A search of PubMed, CINAHL, CENTRAL, EMBASE, KISS, KoreaMed, and RISS was conducted in November 2020. The study included randomized controlled trials. Data were pooled using fixed- and random-effects models. Results: Fourteen studies were identified that cumulatively included 1,548 participants. ACP interventions were effective in alleviating decision conflict (d=-0.53; 95% CI: -0.83 to -0.23), depression (d=-1.22; 95% CI: -1.71 to -0.74) and anxiety (d=-0.76; 95% CI: -1.12 to -0.39). Conclusion: ACP interventions have significant positive effects on reducing decision conflict and psychological distress. A high level of bias was shown related to allocation concealment and blinding. The results of this study are expected to be useful for end-of-life care providers to improve the effectiveness of ACP interventions.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.35 no.1
• /
• pp.159-166
• /
• 2008

The dental caries can be recovered or worse depending on the velocity of de- and remineralization of tooth. It is possible to remineralize the lesions by fluoride agent, but the results can be distinguished according to one's salivary flow rate, quantity of ion contents, and pH of the saliva. This article presents good results after applying the CPP-ACP paste for the patients who have incipient enamel caries. We instructed the patients to apply the paste everyday in the customed tray. We observed the white spot lesions without getting worse and reducing the size of lesions. After applying the CPP-ACP paste for 6 weeks, we concluded that; 1. It was possible to remineralize the incipient caries without preparation of the teeth. 2. CPP-ACP paste was successful for recovery of the demineralized lesions, especially for mild and moderate caries lesions, not for the severe developemental defects or chronic lesions. 3. CPP-ACP paste was efficient for pediatric patients, and the custumed tray was very useful for patients to apply the paste. 4. The ability of the patients for caring their oral hygiene was improved by routine check up and instructions.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.3
• /
• pp.427-433
• /
• 2008

The cephabacins produced by Lysobacter lactamgenus are ${\beta}$-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. In order to understand the precise implication of the polyketide synthase (PKS) module in the biosynthesis of cephabacin, the genes for its core domains, ${\beta}$-ketoacyl synthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), were amplified and cloned into the pET-32b(+) expression vector. The sfp gene encoding a protein that can modify apo-ACP to its active holo-form was also amplified. The recombinant KS, AT, apo-ACP, and Sfp overproduced in the form of $His_6$-tagged fusion proteins in E. coli BL21(DE3) were purified by nickel-affinity chromatography. Formation of stable peptidyl-S-KS was observed by in vitro acylation of the KS domain with the substrate [L-Ala-L-Ala-L-Ala-L-$^3H$-Arg] tetrapeptide-S-N-acetylcysteamine, which is the evidence for the selective recognition of tetrapeptide produced by nonribosomal peptide synthetase (NRPS) in the NRPS/PKS hybrid. In order to confirm whether malonyl CoA is the extender unit for acetylation of the peptidyl moiety, the AT domain, ACP domain, and Sfp protein were treated with $^{14}C$-malonyl-CoA. The results clearly show that the AT domain is able to recognize the extender unit and decarboxylatively acetylated for the elongation of the tetrapeptide. However, the transfer of the activated acetyl group to the ACP domain was not observed, probably attributed to the improper capability of Sfp to activate apo-ACP to the holo-ACP form.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2005.06a
• /
• pp.426-427
• /
• 2005

Advanced Spent Fuel Conditioning Process (ACP) is a pyrochemical process in which the spent fuel of PWR is transformed into the uranic metal ingot. Through this process, which has been developed in KAERI since 1998, the radioactivity, the radiotoxicity, the heat and the volume of the PWR spent fuel are reduced by a quarter of the original. To demonstrate a lab-scale process and extract the data for the later pilot-scale process, a demonstration facility of ACP (ACPF) is under construction and the lab-scale demonstration is slated for 2006. To establish the safeguardability of ACPF, a safeguards system including a neutron counter based on non-destructive assay, which is named as ACP Safeguards Neutron Counter (ASNC), the ACP Safeguards Surveillance System (ASSS) which consists of two neutron monitors and five IAEA cameras, and Laser Induced Breakdown System (LIBS) have been developed and are ready to be installed at ACPF. The target materials of ACP to assay with ASNC are categorized into three types among which the first is the uranic metal ingot, the second is the salt waste and the last is $UO_2$ and $U_{3}O_8$ powders, rod cuts and hulls. The Pu content of process nuclear materials can be accounted with ASNC. The ASSS is integrated in the ACP Intelligent Surveillance Software (AISS) in which the IAEA camera images and background signals at the rear doors of ACPF are displayed. The composition of special nuclear materials of ACP can be measured with LIBS which can be a supporting measurement tool for ASNC. The conceptual picture of safeguards system of ACPF is shown in Fig. 1.