• 한국수산과학회지
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• 제46권1호
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• pp.18-26
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• 2013

This study examined the taste, nutritional and functional characterizations of commercial seasoned sea squirt Halocynthia roretzi (CSS). Total taste values of CSS ranged from 7.6 to 69.5 and the major free amino acids were glutamic acid and aspartic acid. Total contents of amino acids in CSS ranged from 5.91 to 7.59 g/100 g and the major amino acids were also glutamic acid and aspartic acid. When taking 100 g of CSS, the minerals that could be expected to have functional health effects (minerals whose levels were above 10% of the recommended daily requirements) were P, Mg and Fe. Other minerals were also present in non-negligible quantities. In terms of the functional properties of CSS, ACE inhibitory activity was 21.2-37.1%, antioxidative activity was 55.4-90.4%, xanthine oxidase inhibitory activity was 52.9-76.6% and ${\alpha}$-glucosidase inhibitory activity was 0-32%. Antimicrobial activity against Vibrio parahaemolyticus was not detected, but activity against Staphylococcus aureus, groups such as KB, GG, CY, DN, HC and KH, and against Escherichia coli groups such as SF, WD, KB and GG, was detected.

• 한국축산식품학회지
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• 제42권1호
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• pp.46-60
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• 2022

In this study, angiotensin-I-converting enzyme inhibitory (ACEI) activity was evaluated in fermented goat milk fermented by lactic acid bacteria (LAB) from fermented foods and breast milk. Furthermore, the potential for ACEI peptides was identified in fermented goat milk with the highest ACEI activity. The proteolytic specificity of LAB was also evaluated. The 2% isolate was inoculated into reconstituted goat milk (11%, w/v), then incubated at 37℃ until pH 4.6 was reached. The supernatant produced by centrifugation was analyzed for ACEI activity and total peptide. Viable cell counts of LAB and titratable acidity were also evaluated after fermentation. Peptide identification was carried out using nano liquid chromatography mass spectrometry (LC-MS/MS), and potential as an ACEI peptide was carried out based on a literature review. The result revealed that ACEI activity was produced in all samples (20.44%-60.33%). Fermented goat milk of Lc. lactis ssp. lactis BD17 produced the highest ACEI activity (60.33%; IC₅₀ 0.297±0.10 mg/mL) after 48 h incubation, viable cell counts >8 Log CFU/mL, and peptide content of 4.037±0.27/mL. A total of 261 peptides were released, predominantly derived from casein (93%). The proteolytic specificity of Lc. lactis ssp. lactis BD17 through cleavage on the amino acid tyrosine, leucine, glutamic acid, and proline. A total of 21 peptides were identified as ACEI peptides. This study showed that one of the isolates from fermented food, namely Lc. lactis ssp. lactis BD17, has the potential as a starter culture for the production of fermented goat milk which has functional properties as a source of antihypertensive peptides.

• 한국식품영양학회지
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• 제14권6호
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• pp.562-567
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• 2001

In order to utilize okara protein as a food auditive, nutritional composition of soymilk okara was investigated. Protein in okara Is highly insoluble due to excessive heat treatment during soymilk processing. Protein content of okara was 37.3% as compared to 42.5 % for soybean. Carbohydrate and lipid contents of okara were 40.6% and 17.9%, respectively. Okara lipid extracted with chloroform-methanol consisted of neutral lipid, glycolipid and phospholipid, with neutral lipid making up 98.6% . Linoleic acid, ileic acid, and palmitic acids accounted for about 80% of the total fatty acids with linoleic acid sharing 50.3% of the total. Amino acid composition of okara protein was dissimilar to that of soy Protein : Cysteine was totally absent in okara while lysine, which is the limiting amino acid of soy protein, was present in higher amount in okara on dry weight basis. Both aqueous extract of okara protein and soy Protein were found to have ACE inhibitory activity.

• 생약학회지
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• 제12권1호
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• pp.51-54
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• 1981

Twenty-seven medicinal plants were selected on the basis of folkloric reputation for the treatment of hypertension or related deseases. Two solvent fractions were prepared from methanol extract of each plant and tested for their effects on angiotensin converting enzyme (ACE) activities. Six solvent fractions showed more than 50% inhibition and four showed $40{\sim}50%$ inhibition at the conditions tested.

• 한국응용과학기술학회지
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• 제26권4호
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• pp.457-466
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• 2009

This study is manufacturing method and analysis of feasibility about collagen peptide from fish scale. This is processed by enzyme hydrolysis, isolating and refining etc. The results of analysis of nutritional composition showed protein content of collagen peptide. In the analysis of constitutive amino acids, the ratio of contents of hydroxyproline and glycine, the characteristics of collagen peptides appeared similar and the contents of glutamic acid and aspartic acid which are involved in protein metabolism. As a result of measurement of total polyphenol content and total flavonoid, it showed that collagen peptide had more contents generally, and the effect of bioactivity of pig-skin collagen peptide appeared higher although different kinds of scale collagen peptide showed a little DPPH radical scavenging ability, total antioxidant capacity by ABTS, ACE inhibitory.

• 한국응용과학기술학회지
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• 제26권3호
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• pp.248-262
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• 2009

This study is on the feasibility of use of glycoprotein in various areas such as cosmetics and food etc. by extracting, isolating and refining glycoprotein from carrots, red ginseng extract residue, sesame and pine needles using protease(pepsin) and by analyzing general characteristics and measuring various bioactivities. The results of analysis of nutritional composition showed protein contents of glycoprotein. In the analysis of constitutive amino acids, the ratio of contents of hydroxy proline and glycine, the characteristics of glycoproteins appeared similar and the contents of glutamic acid and aspartic acid appeared higher. As a result of measurement contents of total polyphenol and flavonoid, it showed that glycoprotein had more contents generally, and the effect of bioactivity of glycoprotein appeared higher although different kinds of glycoprotein showed a little DPPH radical and nitrite scavenging ability, total antioxidant capacity by ABTS, ACE inhibitory.

• Preventive Nutrition and Food Science
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• 제4권1호
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• pp.14-17
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• 1999

Bovine hide and pig skin gelatins were prepared and their molecular weight profiles were examined by SDS-PAGE. The major molecular weights of bovine hide gelatin were 220 kDa, 140kDa, and 130kDa and the weights of pigskin gelatin were 210 kDa, 135kDa and 120kDa. Also , as a typical parameter of rheological property of the gelatin , viscosities of gelatin were measured under various conditions. Gelatin hydrolysates were prepared using typical commerical proteases and their angiotensin converting enzyme inhibitory activities were examined.

• 한국식품영양학회지
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• 제20권3호
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• pp.276-281
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• 2007

Oddi is a mulberry fruit that has anti-inflammatory and antioxidative effects. This study was carried out to investigate the quality characteristics of mulberry fruit wines manufactured by two different methods. Where they were processed according to the traditional Korean fruit wine methodology(A) or a modified(B). Sensory qualities, physiochemical characteristics, and biochemical activities of the Oddi wines were analyzed. From the results of the sensory evaluation, manufacturing method A showed higher acceptability. However, the two different methods was not significantly different overall. The method A wine had higher brightness(L)(77.65) redness(a), and yellowness(b). As for the antioxidant activities and ACE(angiotensin-converting enzyme) inhibitory abilities of the wines manufacturing method A had greater values.

• 한국식품영양과학회지
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• 제43권11호
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• pp.1701-1708
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• 2014

참취의 활용 및 생리활성을 증가시킬 수 있는 적정 추출방법을 알아보고자 상온교반, 환류냉각, 가압가열, 저온고압 및 초음파 추출법을 이용하여 추출한 두벌 째 수확 참취 추출물의 생리활성을 비교하였다. 추출 수율은 환류냉각추출, 초음파추출, 가압가열추출, 상온추출, 저온고압추출 순으로 높았다. 폴리페놀, 플라보노이드 함량은 저온고압추출에서 유의적으로 높았고, 상압가열, 고온고압, 초음파추출에서는 대등한 함량을 나타내었다. 페놀화합물 정량의 경우 chlorogenic acid 함량은 가압가열추출에서 유의적으로 높았으며 cynarin 함량의 경우 환류냉각, 저온고압 및 초음파 추출에서 유의적으로 높았고, astragalin 함량의 경우 저온고압추출에서 높은 함량이 검출되었다. Xanthine oxidase(XO), angiotensin I-converting enzyme(ACE), HMG-CoA reductase 저해활성의 경우 저온고압추출 및 초음파 추출물에서 우수한 활성을 나타내었으며, 소화효소 저해활성(${\alpha}$-amylase, trypsin, lipase)에서도 유사한 경향을 나타내었다. 이러한 결과를 종합해 볼 때 저온고압 및 초음파 추출물에서 소재 활용가치가 높을 것으로 사료되며 천연 항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1211-1219
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• 2012

골담초에 함유된 페놀성 물질은 70% 에탄올을 용매로 하여 18시간 추출하였을 때 가장 많이 용출되었다. 항산화 효과 중 전자공여능을 측정한 결과는 꽃과 잎 모두 70% 에탄올 추출물에서 74%로 가장 높은 항산화 활성을 나타내었으며, ABTS radical cation decolorization을 측정한 결과, 꽃의 경우 70~90% 에탄올 추출물에서 60% 이상의 항산화 활성을 나타내었다. 지용성 물질에 대한 항산화력으로 antioxidant protection factor(PF)를 측정한 결과, 70% 에탄올 추출물에서 1.2 이상의 PF를 나타내었다. 활성산소 중 지방산화를 일으키는 hydroxyl radical에 대한 골담초 추출물의 영향은 꽃의 물 추출물과 70% 에탄올 추출물에서 대조구보다 낮은 TBARS 값을 나타내었다. ACE에 대한 골담초 추출물의 저해활성은 꽃의 경우 물과 70% 에탄올 추출물 모두 80~99%의 높은 저해활성을 나타내었고, Xoase 대한 저해활성은 꽃보다 잎 추출물의 저해활성이 높았다. 골담초 추출물의 주름개선과 미백효과를 확인한 결과 꽃과 잎 추출물 모두 페놀성 물질을 0.8 mg/mL의 농도로 처리하였을 때 50% 이상의 elastase 저해활성과 70% 에탄올 추출물에서 40.24%로 가장 높은 tyrosinase 저해활성을 나타내었고, 수렴효과는 같은 농도의 tannic acid보다 높은 수렴효과를 나타내었다.