• Journal of Plant Biology
• /
• v.37 no.4
• /
• pp.445-452
• /
• 1994

Effects of methyl jasmonate (MeJA) on ethylene production in mungbean (Phaseolus radiatus L.) hypocotyl and leaf segments were studied. Ethylene production in mungbean hypocotyl segments was decreased in proportion to MeJA concentrations and $450\;\mu\textrm{M}$ of MeJA showed 50% inhibitory effect. This inhibitory effect appeared after 3 h of incubation period and continued for 24 h. Inhibition of ethylene production by MeJA was due to the decrease in 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase activity. However, MeJA treatment had no effect on ACC content and ACC synthase activity. MeJA also inhibited auxin-induced ethylene production in hypocotyls. To investigate the mechanisms of the inhibitory effect of MeJA on the auxin-induced ethylene production, ACC synthase and ACC oxidase activity were examined after MeJA treatment. MeJA decreased the ACC content and ACC synthase activity as weD as ACC oxidase activity in the auxin-treated tissue. These results suggest that the inhibition of MeJA on auxin-induced ethylene production is not due to the direct inhibitory effect of MeJA on the ACC synthase, but to the inhibition of the ability of IAA to promote the synthesis of ACC synthase. In contrast, ethylene production from the detached mungbean leaves was stimulated by MeJA. The rate of ethylene production increased approximately 65% over the control after 12 h of incubation period by $4.5\;\mu\textrm{M}$ MeJA. When MeJA was applied to detached leaves along with IAA, the effect of MeJA appeared to be additive. In an effort to elucidate mechanisms of MeJA action on auxin-induced ethylene production in the leaf tissue, enzyme activities of ACC synthase and ACC oxidase were examined. MeJA stimulated ACC oxidase activity but did not affect ACC synthase activity in leaf tissue. Together, these results suggest that MeJA plays different roles in the ethylene production in the different mungbean tissues.issues.

• Journal of Plant Biology
• /
• v.39 no.1
• /
• pp.31-40
• /
• 1996

The physiological and biochemical characterizations of the ethylene-related mutants in Arabidopsis thaliana - ethylene overproducing mutant (eto1-l) and ethylene insensitive mutants (etrl-3, ein2-l) - were detailed in this studies. Two or three week.old mature rosette leaves (before bolting) were used as the plant materials. Ethylene productions of eto1-l, etrl-3, and ein2-l mutants were about 200%, 400%, and 450% compared to that of wild type, respectively. ACC synthase and ACC oxidase activities of eto1-l mutant were similar to those of wild type. ACC content and ACC N-malonyltransferase activity, however, were 4.5 times and 3 times higher than those of wild type, respectively. SAM synthetase activity increased by 50% in eto1-l mutant plant. These results indicated that the alteration in the eto1-l mutant occured before the step of the conversion of SAM to ACe. In etrl-3 and ein2-l mutants, ACC synthase activities increased, but ACC oxidase activities decreased. ACC content and ACC N-malonyltransfcrase activity were 2 times higher than those of wild type. SAM synthetase activity in etrl-3 is similar to those of wild type, while it increased by 73% in ein2-l. These results showed that the block in ethylene action affected the autoregulation of ethylene biosynthesis, so that ACC synthase activity was not autoinhibited and ACC oxidase activity was not auto stimulated by ethylene. When the leaf tissues were used for in vitro kinase assay, a cytosolic protein (approximately 36 kDa) was phosphorylated only in eto1-l and ein2-l mutants.utants.

• Journal of Plant Biology
• /
• v.33 no.4
• /
• pp.309-314
• /
• 1990

Effect of mannose on auxin-induced ethylene production in corn (Zea mays L.) coleoptiles was studied. Auxin induced ethylene production decreased in proportion to mannose concentrations. The inhibitory effect of mannose appeared after 2 h of incubation. Ethylene production was significantly depressed by mannose at high concentration (10-5M-10-4M) of indole acetic acid (IAA), but not at low concentrations (10-8M-10-6M). The inhibition of auxin-induced ethylene production by mannose was specific, since other sugars such as galactose, glucose, sucrose and mannitol did not have an inhibitory effect. In an effort to elucidate mechanisms of mannose the effect on the auxin induced ethylene production, effect of the sugar on ACC synthase activity and ACC induced ethylene production was studied. Mannose failed to inhibit ACC mediated ethylene production, but decreased both the ACC content and ACC synthase activity in the tissue. These results suggest that the inhibitory effect of mannose on auxin induced ethylene production results from suppression of auxin induction of ACC synthase.

• Journal of Environmental Science International
• /
• v.23 no.11
• /
• pp.1821-1826
• /
• 2014

The adsorption characteristics of the methylene blue (MB) were studied using three activated carbons such as ACA and ACB with similar specific surface area (1,185 and $1,105m^2/g$), and ACC with relatively high specific surface area ($1,760m^2/g$). The surface chemical properties of these activated carbons were investigated by X-ray photoelectron spectroscopy (XPS). The results indicated that ACA had more functional groups (with phenol, carbonyl, and carboxyl etc.) than ACB (with carbonyl and carboxyl) and ACC (with carboxyl). The isotherm data were fitted well by Langmuir isotherm model. The adsorption capacities of ACA, ACB, and ACC for MB were 454.7 mg/g, 337.7 mg/g, and 414.0 mg/g, respectively. As phenol and carboxyl content of the surface on activated carbon increased, MB adsorption capacity was increased. Although ACA had a smaller specific surface area than ACC, the content of phenol and carboxyl group was abundant, so MB adsorption capacity was found to be higher than ACC.

• Horticultural Science & Technology
• /
• v.29 no.4
• /
• pp.326-335
• /
• 2011

When bougainvilleas are subjected to indoor low-light conditions, flower bracts regularly abscise. This study elucidates the effects of plant growth regulators on bract longevity of potted bougainvillea. Potted 'Taipei Red' bougainvillea in four different bract development stages were treated with 1-MCP (1-methylcyclopropene), NAA (1-naphthaleneacetic acid), SNA (sodium salt of naphthaleneacetic acid), IBA (indolebutyric acid), BA (6-benzylaminopurine), $KH_2PO_4$ (potassium dihydrogen phosphate), Put (diamine putrescine), SA (salicylic acid), or STS (silver thiosulfate) and were moved to indoor low-light conditions after treatments. Experimental results indicate that 1-MCP, NAA, SNA, BA, Put, and SA prolonged bract longevity, and this effect increased as bract stage increased. The effect of STS was significant in early bract stages and decreased as bract stages increased. Additionally, 1-MCP, NAA, SNA, BA, Put, SA, and STS treatment significantly reduced endogenous ACC (1-aminocyclopropene-1-carboxylate) content and ACC oxidase activity, suggesting that the inhibition of ethylene production was achieved via physiological metabolism. However, treatment with IBA or $KH_2PO_4$ had no effect on the bract longevity at any stage. In the combined chemical treatments, NAA + STS or NAA + SA were effectively for prolonging bract longevity and contained less protein or chlorophyll degradation, decrease ACC oxidase or ethylene production than the control. In conclusion, we propose that combined chemical treatment significantly prolonged the bract longevity and more effectively than single chemical treatment at any stage.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• v.48 no.4
• /
• pp.27-34
• /
• 2011

In this paper, it was presented a design on the dynamic multimedia fingerprinting code for anti-collusion code(ACC) in the protection of multimedia content. Multimedia fingerprinting code for the conventional ACC, is designed with a mathematical method to increase k to k+1 by transform from BIBD's an incidence matrix to a complement matrix. A codevector of the complement matrix is allowanced fingerprinting code to a user' authority and embedded into a content. In the proposed algorithm, the feature points were drawing from a content which user bought, with based on these to design the dynamical multimedia fingerprinting code. The candidate codes of ACC which satisfied BIBD's v and k+1 condition is registered in the codebook, and then a matrix is generated(Below that it calls "Rhee matrix") with ${\lambda}+1$ condition. In the experimental results, the codevector of Rhee matrix based on a feature point of the content is generated to exist k in the confidence interval at the significance level ($1-{\alpha}$). Euclidean distances between row and row and column and column each other of Rhee matrix is working out same k value as like the compliment matrices based on BIBD and Graph. Moreover, first row and column of Rhee matrix are an initial firing vector and to be a forensic mark of content protection. Because of the connection of the rest codevectors is reported in the codebook, when trace a colluded code, it isn't necessity to solve a correlation coefficient between original fingerprinting code and the colluded code but only search the codebook then a trace of the colluder is easy. Thus, the generated Rhee matrix in this paper has an excellent robustness and fidelity more than the mathematically generated matrix based on BIBD as ACC.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.3
• /
• pp.288-298
• /
• 1992

Since the major important factors limiting plant growth and crop productivity are environmental stresses, of which low temperature is the most serious. It has been well known that many physiological processes are alterant in response to the environmental stress. With regard to the relationship between plant hormones and the regulation of chilling tolerance in rice seedlings, the major physiological roles of plant hormones: abscisic acid, ethylene and polyamines are evaluated and discussed in this paper. Rice seedlings were grown in culture solution to examine the effect of such plant hormones on physiological characters related to chilling tolerance and also to compare the different responses among tested cultivars. Intact seedlings about 14 day-old were chilled at conditions of 5$^{\circ}C$ and 80% relative humidity for various period. Cis-(＋)-ABA content was measured by the indirect ELISA technique. Polyamine content and ethylene production in leaves were determined by means of HPLC and GC respectively. Chilling damage of seedlings was evaluated by electrolyte leakage, TTC viability assay or servival test. Our experiment results described here demonstrated the physiological functions of ABA, ethylene, and polyamines related to the regulation of chilling tolerance in rice seedlings. Levels of cis-(＋)-ABA in leaves or xylem sap of rice seedlings increased rapidly in response to 5$^{\circ}C$ treatment. The tolerant cultivars had significant higher level of endogenous ABA than the sensitive ones. The ($\pm$)-ABA pretreatment for 48 h increased the chilling tolerance of the sensitive indica cultivar. One possible function of abscisic acid is the adjustment of plants to avoid chilling-induced water stress. Accumulation of proline and other compatible solutes is assumed to be another factor in the prevention of chilling injuies by abscisic acid. In addition, the expression of ABA-responsive gene is reported in some plants and may be involving in the acclimation to low temperature. Ethylene and its immediate precusor, 1-amincyclopropane-1-carboxylic acid(ACC) increased significantly after 5$^{\circ}C$ treatment. The activity of ACC synthase which converts S-adenosylmethionine (SAM) to ACC enhanced earlier than the increase of ethylene and ACC. Low temperature increased ACC synthase activity, whereas prolonged chilling treatment damaged the conversion of ACC to ethylene. It was shown that application of Ethphon was beneficial to recovering from chilling injury in rice seedlings. However, the physiological functions of chilling-induced ethylene are still unclear. Polyamines are thought to be a potential plant hormone and may be involving in the regulation of chilling response. Results indicated that chilling treatment induced a remarkable increase of polyamines, especially putrescine content in rice seedlings. The relative higher putrescine content was found in chilling-tolerant cultivar and the maximal level of enhanced putrescine in shoot of chilling cultivar(TNG. 67) was about 8 folds of controls at two days after chilling. The accumulation of polyamines may protect membrane structure or buffer ionic imbalance from chilling damage. Stress physiology is a rapidly expanding field. Plant growth regulators that improve tolerance to low temperature may affect stress protein production. The molecular or gene approaches will help us to elucidate the functions of plant hormones related to the regulation of chilling tolerance in plants in the near future.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.30 no.3
• /
• pp.236-244
• /
• 1985

To know the varietal differences of ethylene evolution, 55 rice varieties were tested at seedling stage. And, also, 6 rice varieties were tested the biosynthesis of ethylene production. The group of japonica varieties produced higher amount of ethylene than the indica and indica x japonica crossed varieties. The content of ACC in rice seedlings of japonica varieties was higher than in seedlings of indica and indica x japonica varieties. And, also, the conversion rate of mathionine and ACC to ethylene in seedling of japonica varieties was higher than in seedling of indica and indica x japonica varieties.

• Horticultural Science & Technology
• /
• v.33 no.3
• /
• pp.356-363
• /
• 2015

This study examined the effects of different day/night temperature regimes or silver ion on growth and flowering of passion fruit 'Tai-nung No.1'. Low temperature treatment ($20/15^{\circ}C$) caused passion fruit cultivar 'Tai-nung No.1' to fail to flower. Flowering induction occurred within a temperature range of $20-30^{\circ}C$, with no significant difference in the days to first flower bud and the total number of flower buds between plants grown at $30/25^{\circ}C$ and $25/20^{\circ}C$. However, plants grown at $30/25^{\circ}C$ exhibited their first flower buds set on the higher nodes and had higher abortion rates of flower buds than those at $25/20^{\circ}C$. Plants grown at $30/25^{\circ}C$ had the most rapid growth and the shortest plastochron. We also evaluated the effect of the ethylene response inhibitors silver nitrate ($AgNO_3$) and silver thiosulfate (STS) on growth and flowering of potted passion fruit 'Tai-nung No.1', when they were exposed to low temperature conditions ($20/15^{\circ}C$) following chemical treatments ($AgNO_3$ or STS, at 0.5 or 1.0 mM). $AgNO_3$ and STS treatments induced flower formation and initial flower bud formation within approximately two weeks at $20/15^{\circ}C$ whereas non-treated control plants exhibited no flower formation. ACC content and activity of ACC oxidase in the leaves of passion fruit 'Tai-nung No.1'exposed to low temperature conditions ($20/15^{\circ}C$) were significantly inhibited by the ethylene inhibitor treatments. These results indicate that ethylene, which is produced under low temperature conditions, plays an important role in inhibiting flower formation in passion fruit.

• Horticultural Science & Technology
• /
• v.19 no.4
• /
• pp.545-549
• /
• 2001

The relationship between ethylene action and flesh softening in post-storage persimmon fruits was investigated by treating the fruits with 1-MCP. The patterns of firmness change caused by various treatments with 1-MCP and ethylene were similar to those of changes in cell wall-degrading enzyme activities, including cellulase, PG, PME, and ${\beta}$-galactosidase. Moreover, the activities of these enzymes were inhibited by 1-MCP treatment. These results show that the cell wall-degrading enzymes influenced by ethylene account for the flesh softening process of post-storage persimmon fruit. The ethylene production of fruits, as measured by ACC content and ACC oxidase activity, nevertheless, was not influenced by 1-MCP treatments. It is suggested that the flesh softening phenomena in post-storage persimmon fruits is correlated to the ethylene responsiveness of tissue rather than the ethylene production rate per se.