• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.270-270
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• 2017

Brown rice flour (BRF) was treated with different carbohydrases (Viscozyme, Termamyl, Celluclast, AMG, Ultraflo, and Pentopan), and then aqueous alcoholic extracts (70% ethanol) from the treated RBF were examined for their phenolic compositions and antioxidant activities (ABTS and DPPH radical scavenging activity). All the carbohydrases tested induced significant increases in ABTS radical scavenging activity (2.1-3.0 times). Moreover, These enzymes increased the amount of extractable free phenolic acids by 10-15 times, especially for ferulic and p-coumaric acid. Among the enzymes tested, Pentopan which was active in arabinoxylan hydrolysis appeared to be most effective in increasing the free phenolic acid content and ABTS radical scavenging activity than other enzymes. Enzymatic hydrolysis of cell wall polysaccharides in BRF could be used as an effective procedure for raising the amount of extractable phenolic acids and thus increasing the antioxidant activity of BRF extract.

• 대한본초학회지
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• 제33권6호
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• pp.29-34
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• 2018

Objectives : It is necessary to manage herbal medicines based on effectiveness by comparing the efficacy of herbal medicines by region. In this study, we compared anti-oxidative activity and marker compounds of Eucommiae Cortex by regional groups. Methods : Eucommiae Cortex grown and harvested in Gangjin, Sancheong, Yeongwol, Jangsu, and Jecheon were used. Eucommiae Cortex was extracted in distilled water at $100^{\circ}C$ for 3 hours, and filtered. filtered. Extract samples were freeze-dried at $-80^{\circ}C$. Comparison of anti-oxidant activity in Eucommiae Cortex samples from regional groups was measured in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP) between regional groups of Eucommiae Cortex. In addition, high-performance liquid chromatography (HPLC) analysis was conducted to compare pinoresinol diglucoside concentration by regional groups. Results : HPLC analysis found that pinoresinol diglucoside concentration, widely known as the marker compound of Eucommiae Cortex, was the highest in Gangwon Yeongwol. There was a significant difference in anti-oxidative activity of Eucommiae Cortex between regional groups as discovered in DPPH, ABTS and FRAP assays. DPPH free radical scavenging was the highest in Jeonbuk Jangsu. ABTS free radical scavenging was the highest in Jeonbuk Jangsu and Chungbuck Jecheon. FRAP was the highest in Jeonbuk Jangsu. Conclusions : Although pinoresinol diglucoside concentration was high, anti-oxidative activity was not proportionately high. Pinoresinol diglucoside concentration was the highest in Gangwon Yeongwol. Anti-oxidative activity was the highest in Jeonbuk Jangsu.

• 한국조리학회지
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• 제21권2호
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• pp.41-51
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• 2015

본 연구는 이용도가 높은 녹두의 국내 생산 부족으로 수입이 증가되고 있는 중국산 녹두의 천연 항산화성 소재로 활용시 기초자료를 제공하고자 하였으며, 녹두의 메탄올 추출물의 페놀성 화합물 함량과 in vitro 항산화 모델(DPPH 라디칼 소거능, ABTS 라디칼 소거능)을 통하여 항산화 활성을 측정하였다. 중국산 녹두 추출물의 총 페놀 및 플라보노이드 함량은 각각 $174.83{\pm}2.90GAE\;mg/g$ 및 $68.87{\pm}2.84QE\;mg/g$으로 나타났다. 중국산 녹두 추출물의 DPPH 라디칼 소거능과 ABTS 라디칼 소거능은 농도 의존적으로 유의적으로 증가하였으며(p<0.05), 초기 ABTS 라디칼의 50%를 소거하는데 필요한 농도($IC_{50}$)는 2.85 mg/mL로 나타났다. 이상의 결과로부터, 중국산 녹두는 페놀성 화합물이 풍부하며, 천연물 유래 항산화성 소재로서 활용 가능성이 높은 것으로 기대된다.

• 한국약용작물학회지
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• 제17권1호
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• pp.15-20
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• 2009

This study was carried out in order to determine the biological activities such as antioxidant activities in nine cultivars of Momordica charantia L. (Korea, China, Japan and Philippine native cultivars). Their antioxidant activities were measured using DPPH free radical scavenging, ABTS cation radical scavenging, and Nitrite scavenging ability. The highest total polyphenol content (16.82 ${\mu}g/m{\ell}$) measured in the native Korea cultivar, and this value was 4.0 ${\mu}g/m{\ell}$ higher than that of the 'Verde Beuhas' cultivar (12.82 ${\mu}g/m{\ell}$). The 'Peacock' cultivar had the highest total flavonoid amount which was 4.38 ${\mu}g/m{\ell}$. The free radical scavenging activity using DPPH method was the highest in the native China cultivar ($RC_{50}$ = 102.6 ${\mu}g/m{\ell}$). ABTS cation radical scavenging activity according to cultivars was significantly higher in 'Peacock'. Nitrite scavenging ability showed the most remarkable effect at the pH 1.2, exhibited to 81.5${\sim}$86.9% by addition of ethanol extract 1${\mu}g/m{\ell}$ from Momordica charantia L. These results suggest that Momordica charantia L. had the potent biological activities, and that their activities exhibited differently depending on cultivars.

• 대한화장품학회지
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• 제35권3호
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• pp.229-233
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• 2009

식물 20종을 메탄올로 추출하여 양성자 빔 조사 에너지양(1, 5, 10 KGray)에 따른 항산화 활성 능을 탐색 하고자 DPPH 라디칼 소거활성과 ABTS free 라디칼 소거 활성에 대하여 조사하였다. 양성자 빔 조사 시 15종류는 활성이 증가하였으나 10 KGray에서 흑축(Pharbitis nil Choisy)은 활성이 감소하였으며, 4종류는 활성변화가 없었다. DPPH 라디칼 소거활성은 1 및 5 KGray에서 유근피(Ulmus macrocarpa) (84 %), 10 KGray (85 %)로 가장 높았으나 10 KGray에서 흑축(Pharbitis nil Choisy)은 6 % 감소하였다. $IC_{50}$값을 무처리구와 빔 조사량에 대한 활성을 비교하면 1 KGray에서 산약(Dioscorea batatas Decne.) 6.3배, 5 KGray에서 천화분(Trichosanthes kirilowii Max.) 2.1배, 산약(Dioscorea batatas Decne.) 2.8배 증가하였다. ABTS free 라디칼 소거활성에서 1 KGray의 양성자 빔 에너지를 조사 했을 경우, 가자(Terminalia chebula Retzius)는 60 % 증가하였다. $IC_{50}$값은 1 KGray 빔 조사 시 0 KGray보다 마황근(Ephedra sinica Stapf) 2.0배, 가자(Terminalia chebula Retz.) 2.5배, 우방자(Arctium lappa Linne) 2.4배 증가하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.184-184
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• 2022

This study was aimed to evaluate the antioxidant activity and bioactive property of extracts from a purple-colored wheat variety 'Arriheuk' (Triticum aestivum L.) wheatgrass, affected by light emitting diode irradiation (LED). The wheatgrass was cultivated for 10 days after sowing in a growth chamber under the following LED conditions: R1B1 (Red:Blue = 1:1), R7B3 (Red:Blue = 7:3), and R3B7 (Red:Blue = 3:7). We examined antioxidant activity of the hot water extracts of wheatgrass using DPPH and ABTS free radicals scavenging assays. At the concentration of 10,000 ㎍/ml, the extract from R1B1 showed the highest DPPH free radical scavenging activity(79.29%), but its ABTS free radical scavenging activity was the lowest(32.0%). To evaluate bioactive property of the wheatgrass, we examined the change of natural killer (NK) cell activity affected by the wheatgrass extracts in vitro. At the concentration of 500㎍/ml, NK cell activity was most highly enhanced by the extract from R1B1(181.6%), and the activity was 176.1% (R7B3) and 144.6%(R3B7), respectively. These results suggest that the functional property of 'Arriheuk' wheatgrass would be enhanced by LED irradiation condition.

• Food Science and Biotechnology
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• 제17권4호
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• pp.846-849
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• 2008

This study employed high performance liquid chromatography (HPLC) coupled to an on-line $ABTS^+$ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled $ABTS^+$-RSD system. The negative peaks of the $ABTS^+$-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-$ABTS^+$-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.

• 한국식품영양학회지
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• 제26권4호
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• pp.857-864
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• 2013

본 연구에서는 열처리가 야콘의 항산화 활성에 미치는 영향을 살펴보기 위하여 열처리 온도(100 및 $121^{\circ}C$)와 시간(15, 30 및 60분)에 따른 항산화 성분 및 항산화 활성 변화를 조사하였다. 열처리 후 야콘의 갈변도, 유리형 및 결합형 폴리페놀 함량, 유리형 및 결합형 플라보노이드 함량, DPPH radical 소거 활성 및 ABTS radical 소거 활성을 측정하였다. 열처리 후 야콘의 갈변도, 유리형 폴리페놀 및 플라보노이드 함량과 항산화 활성은 열처리 온도와 시간에 따라 유의적(p<0.05)으로 증가하였고, 결합형 폴리페놀 및 플라보노이드 함량은 감소하였다. 야콘의 유리형 폴리페놀 및 플라보노이드 함량은 $121^{\circ}C$, 60분 열처리 시 생야콘에 비해 각각 1.2배 및 1.1배로 유의적(p<0.05)으로 증가하였다. 또한, 야콘의 DPPH radical 소거 활성 및 ABTS Radical 소거 활성도 $121^{\circ}C$, 60분 열처리 시 생야콘에 비해 각각 1.7배 및 2.0배로 유의적(p<0.05)으로 증가였다. 야콘의 갈변도, 폴리페놀 및 플라보노이드 함량과 DPPH radical 및 ABTS radical 소거 활성 간의 상관관계를 분석한 결과, 높은 상관관계(p<0.01)가 있는 것으로 나타났다. 본 연구결과, 열처리 방법을 통해 야콘의 항산화 성분과 항산화 활성을 강화시킬 수 있으며, 이를 활용한 기능성 식품 소재 개발이 가능할 것으로 생각된다.

• 산업식품공학
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• 제15권1호
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• pp.6-14
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• 2011

The polysaccharides from fruit body of Auricularia auricula and Tremella fuciformis were extracted using hot water, and partially purified through ethanol precipitation and dialysis. Free radical scavenging activities of the crude and purified polysaccharides were examined and compared each other. Free radical scavenging activities of the partially purified polysaccharides were higher than those of crude polysaccharides. DPPH free radical, ABTS radical and SOD-like activities of partially purified polysaccharide at 1 mg/mL of concentration from A. auricula were 61.7, 9.6 and 38.9%, respectively, while those of T. fuciformis were 9.6, 5.7 and 15.3%, respectively. Results of site and non-site specific hydroxyl radical scavenging activities indicated that the partially purified polysaccharide fractions from A. auricula and T. fuciformis exhibited the hydroxyl radical scavenging effect by hydrogen donating ability and iron ion chelating ability. Also, reducing powers of A. auricula and T. fuciformis were 77.1 and 14.7% of BHT (0.1%) as standard, respectively. It was suggested that antioxidant activities of A. auricula were about 1.4~6.4 times higher than those of T. fuciformis due to different levels of polyphenol content.

• Journal of Dairy Science and Biotechnology
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• 제39권4호
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• pp.157-165
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• 2021

This study aimed to determine the effect of extraction temperature and time on the antioxidant activity of hot water extract from Panax ginseng sprout powder and to evaluate the suitability of this extract for use in dairy products. Water-soluble fractions of commercial Panax ginseng sprout powder were obtained by hot water extraction at 25, 60, or 80℃ for 0.5, 2, 12, or 24 h. The antioxidant activity of each extract was evaluated by measuring its free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). DPPH and ABTS free radical scavenging activity increased with extraction temperature from 25 to 80℃. At 80℃, increasing the extraction time from 0.5 to 2 h led to increases in DPPH and ABTS free radical scavenging activity. Thus, the extract obtained under 2 h at 80℃ was selected for addition to milk and yogurt. After 16 days of storage, there were no significant changes in the pH of the milk or the antioxidant activity of the extract. With regard to yogurt fermentation, adding the extract did not affect the pH or the number of viable lactic acid bacteria. In conclusion, hot water extract from Panax ginseng sprout powder can be added to dairy products to enhance antioxidant activity.