• 한국해양공학회지
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• 제36권4호
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• pp.211-216
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• 2022

Since liquefied natural gas (LNG) is imported in a liquid state of about -162℃ to increase transportation efficiency in Korea, it must be vaporized in a gaseous state to supply it to consumers. Among them, ambient air vaporizer (AAV) has caught attention due to eco-friendly and low costs characteristics. However, there is a disadvantage that the performance of the heat exchanger is deteriorated due to frost due to mist and icing when used for a long time. In this paper, frost generation model in AAV vaporizer was investigated with numerically to examine utilizing the vaporizer performance with the frost generation behavior. The frost generation behavior of AAV vaporizers was examined with humidity, fin characteristic, and temperature effects. As for the LNG discharge temperature, the 12 fin vaporizer showed the highest discharge temperature when the atmospheric temperature was 25℃, and the 8 fin vaporizer had the lowest LNG discharge temperature when the atmospheric temperature was 0℃. In the case of frost formation, in the case of the 12 fin vaporizer, it was formed the most at the atmospheric temperature of 25℃, and the least was formed in the vaporizer at the 0℃ condition of the atmospheric temperature of 8 fins.

• BMB Reports
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• 제42권1호
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• pp.59-64
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• 2009

Hepatitis B virus (HBV) infection is highly prevalent worldwide. The major challenge for current antiviral treatment is the elevated drug resistance that occurs via rapid viral mutagenesis. In this study, we developed AAV vectors to simultaneously deliver two or three shRNAs targeting different HBV-related genes. These vectors showed markedly better antiviral effects than ones that delivered a single shRNA in vitro. A dual shRNA expression vector (AAV-157i/1694i), which simultaneously expressed two shRNAs targeted the S and X genes of HBV, reduced HBsAg, HBeAg and HBV DNA levels by $87{\pm}4$, $80.3{\pm}2.6$ and $86.2{\pm}7%$ respectively, eight days post-transduction. In a mouse model of prophylactic treatment, HBsAg and HBeAg were reduced to undetectable levels and the serum HBV DNA level was reduced by at least 100 fold. These results indicate that AAV-157i/1694i generates potent anti-HBV effects and that the strategy of constructing multi-shRNA expression vectors may lead to enhanced anti-HBV efficacy and overcome the evading mechanism of the virus and thus the development of drug resistance.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4031-4036
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• 2012

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

• 대한유전성대사질환학회지
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• 제5권1호
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• pp.9-17
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• 2005

Homocystinuria is a metabolic disorder caused by a deficiency of cystathionine ${\beta}$-synthase (CBS). Patients with homocystinuria show clinical symptoms such as mental retardation, lens dislocation, vascular disease with life-threatening thromboembolisms and skeletal deformities. Generally, the major treatments for CBS deficiency include pharmacologic doses of pyridoxine or dietary restriction of methionine. However, there is no effective treatment for this disease up till today and gene therapy can be an attractive novel approach to treatment of the disease. We investigated whether a recombinant adeno-associated virus could be used as a CBS gene transfer vector to reduce the excessive homocysteine level in the homocystinuria mouse model. Recombinant adeno-associated virus vector encoding the human CBS gene (rAAV-hCBS), driven by EF1-a promoter, was infused into CBS-deficient mice ($CBS^{-/-}$) via intramuscular (IM) and intraperitoneal (IP) injection. IP injection was more efficient than IM injection for prolongation of lives and reduction of plasma homocysteine levels. After 2 weeks of gene transfer by IP injection, serum homocysteine level was significantly decreased in treated mice compared with the age-matched controls and the life span was extended about 1.5 times. Also, increased expression of CBS gene was observed by immunohistochemical staining in livers of treated $CBS^{-/-}$ mice and microvesicular lipid droplets was decreased in cytoplasm of liver. These results demonstrate the possibility and efficacy of gene therapy by AAV gene transfer in homocystinuria mice.

• 한국수정란이식학회지
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• 제25권1호
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• pp.35-42
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• 2010

Many studies propose that dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I) is associated with neurodegenerative disorders, such as Parkinson's disease and Huntington's disease. Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. With a recombinant adeno-associated virus vector carrying the NDI1 gene (rAAV-NDI1) as the gene delivery method, we were able to attain high transduction efficiencies even in the human epithelial cervical cancer cells that are difficult to transfect by lipofection or calcium phosphate precipitation methods. Using a rAAV-NDI1, we demonstrated that the Ndi1 enzyme is successfully expressed in HeLa cells. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced HeLa cells were not affected by rotenone which is inhibitor of complex I, but was inhibited by flavone and antimycin A. The NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. In particular, in the NDI1-transduced cells, the yeast enzyme becomes integrated into the human respiratory chain. It is concluded that the NDI1 gene provides a potentially useful tool for gene therapy of mitochondrial diseases caused by complex I deficiency.

• 전자공학회논문지SC
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• 제48권6호
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• pp.43-50
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• 2011

바이오인식 기술은 패스워드나 IC 카드와 같이 분실의 우려가 없어 다양한 분야에 응용되고 있으나, 변조가 가능하며 측정방식에 따라 측정자에게 거부감을 줄 수 있다는 단점을 가지고 있다. 이러한 문제점을 해결하기 위해 심전도(ECG)를 이용한 바이오인식 기술에 대한 연구가 진행되고 있으나, 기존의 심전도 바이오인식기술은 심장질환을 측정하는 정형화된 심전도 리드 II 파형을 이용했기 때문에 바이오인식에는 적합하지 못했다. 따라서 본 논문에서는 심전도 리드 III 파형을 이용한 새로운 바이오인식 기술을 제안한다. 측정된 심전도 리드 III 파형은 잡음을 제거하기 위해 필터링을 한 후 AAV 알고리즘을 이용하여 파형의 정점을 찾고, 그 정점을 기준으로 원신호에서 파형을 분류하였다. 추출된 파형을 4가지 타입으로 정의하고 그를 기반으로 꼭짓점 및 세부파형모양, 파형진폭 및 간격 등 총 22가지의 특징들을 추출하였다. 추출된 특징은 오류역전파 신경회로 망인식기를 통해 분류되었다. 심전도 리드 III 파형을 이용한 바이오인식을 위해 31명의 측정자와 데이터베이스에 없는 5명의 측정자, 총 36명을 대상으로 심전도 바이오인식을 실험한 결과 특이도(specificity) 100%, 민감도(sensitivity) 95.59%, 정확도(accuracy) 99.17%의 특성을 보였다.

• 산학경영연구
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• 제13권
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• pp.263-273
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• 2000

벤츠(현 다임-클라이슬러)자동차회사는 새로운 공장을 미국 앨라배마 주 Vance 지역에 건설하고 이곳 공장으로부터 스포츠-레저용의 4륜 구동 M-class 자동차를 생산하고 있다. 이 벤츠공장의 생산라인은 차체형성공정, 도장공정, 그리고 엔진조립 공정으로 나누어져 있고 이들 생산라인사이에는 한 라인에서의 고장(down)이 다른 라인에 파급되지 않도록 완충라인(Buffer)이 설치되어 있다. 본 연구의 목적은 일정한 비율의 고장률을 각 생산라인에 적용시켰을 때 주어진 생산목표(270대)를 달성할 수 있는지, 만약 달성할 수 없다면 가장 문제가 되는 생산라인은 어디인지, 또 생산라인 사이에 존재하는 완충라인이 어느 정도의 완충역할을 하는지, 최적의 생산능력을 유지하기 위한 완충라인의 크기는 어느 정도인지 등, 각종 생산라인의 운영지침(operation policy)들을 시뮬레이션 기법을 이용하여 탐색한 후 병목생산시설(bottleneck)을 찾아내는 것이다. 본 연구를 위한 시뮬레이션 도구로는 ARENA를 이용했고 20일 간의 시뮬레이션 결과를 종합하여 분석하였다. 또 신뢰구간 95% 범위에서 각 생산라인의 최대 생산능력도 측정하여 실제 생산대수와의 차이를 규명할 수 있는 근거를 제공하였다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2006년도 하계종합학술대회
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• pp.791-792
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• 2006

본 논문은 심전도의 리드III 파형을 이용하여 신원확인이 가능한 생체인식 기술을 제안한다. 인식을 위한 심전도의 리드III파형을 특징추출하기 위해 $4{\sim}30Hz$의 대역통과 필터를 사용하여 피크(peak)점만 남겨놓고 모든 잡음을 제거한 후, AAV(absolute amplitude value)를 이용하여 피크점의 값을 추출한다. 추출된 피크 점은 원신호의 피크점과 같으므로 이를 기준으로 전체파형을 특징추출을 위한 단위 파형으로 분리한다. 분리된 신호는 정의된 4가지 형태(type)의 파형 중 가장 유사한 파형타입으로 분류되며, 분류된 형태를 기준으로 꼭지점, 최대 피크점, 최소 피크점, 최대.최소 피크점 비, 파형 간격(interval) 및 파형의 세부 모양 등 총22가지의 특징들을 추출한다. 추출된 특징들은 오류역전파 신경회로망(back-propagation neural network)의 입력으로 사용되었으며, 성인남녀 31명을 대상으로 제한된 파형 내에서 실험한 결과 100%의 인식률을 보였다.

• 생명과학회지
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• 제18권10호
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• pp.1395-1399
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• 2008

우리는 최근에 PRC1 프로모터가 유방암 유전자치료를 위하여 전사 표적이 된 유전자의 발현을 조절할 수 있는 후보 프로모터임을 보고하였다. 우리는 본 실험에서 PRC1이 폐암유전자 치료에서도 적용이 가능한지 조사하였다. 특정 프로모터가 루시퍼라제 유전자와 연결된 플라스미드를 이용한 형질전환 assay에서 PRC1 프로모터는 정상폐세포주에서는 활성을 보이지 않으나 폐암세포주에선 약 30 배의 활성을 보였다. 이는 이미 암특이적인 발현으로 알려진 BIRC5 (survivin) 프로모터와 유사한 결과였다. 또한, 바이러스 벡터를 이용한 실험에서 PRC1은 CMV 프로모터에 비해 아데노부속바이러스에서 약 75%, 아데노바이러스에서 약 66%의활성을 보였다. 이와 대조적으로, PRC1 프로모터를 함유한 이 들 두 종류의 바이러스는 정상 폐세포에서는 20%정도의 낮은 활성을 보였다. 흥미롭게도, 인간 폐종양세포를 이식한 생쥐모델을 사용한 결과에서는 PRC1 프로모터가 CMV 프로모터와 비슷한 생체 활성을 보였다. 종합하면, 이상의 결과는 PRC1이 폐암 유전자치료를 위한 전사표적 유전자의 발현을 위한 프로모터로 사용 가능함을 암시한다.

• Membrane and Water Treatment
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• 제2권3호
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• pp.187-205
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• 2011

The electric transport of the mixture of hydrochloric and phosphoric acids through strong base (Neosepta ACM) and weak base (Selemion AAV) anion-exchange membranes was investigated. The instantaneous efficiency of HCl removal from the cathode solution, $CE_{Cl}$, with and without $H_3PO_4$ was determined. It was found that $CE_{Cl}$ was 0.8-0.9 if the number of moles of elementary charge passed through the system, $n_F$, did not exceed ca. 80% of the initial number of HCl moles in the cathode solution, $n_{Cl,ca,0}$. The retention efficiency of $H_3PO_4$ in that range was close to one. The transport of acid mixtures was satisfactorily described by a model based on the extended Nernst-Planck and Donnan equations for $n_F$ not exceeding $n_{Cl,ca,0}$. Among the tested model parameters, most important were: concentration of fixed charges, the porosity-tortuosity coefficient, and the partition coefficient of an undissociated form of $H_3PO_4$. For the both membranes, the obtained optimal values of fixed charge concentration, $\bar{c}_m$, were up to 40% lower than the literature values of $\bar{c}_m$ obtained from the equilibrium measurements. Regarding the $H_3PO_4$ equilibria, it was sufficient to consider $H_3PO_4$ as a monoprotic acid.