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Biological Control of the Sciarid Fly, Lycoriella mali (Diptera: Sciaridae) Using Steinernema carpocapsae in a Button Mushroom Cultivation House (양송이버섯 재배사에서 Steinernema carpocapsae를 이용한 긴수염버섯파리 생물적 방제)

  • Choi, Yong-Seok;Seo, Hwa-Young;Whang, In-Su;Lee, Dae-Hong
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.355-359
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    • 2018
  • We used the entomopathogenic nematode, Steinernema carpocapsae, for the effective biological control of Lycoriella mali during Agaricus bisporus cultivation. The concentrations of two S. carpocapsae products distributed in Korea were $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ respectively. As a result of investigating the density of A. bisporus larvae in a rice straw medium and soil in an A. bisporus cultivation house, the mean numbers per $30cm^2$ were 0.8 and 22.2, respectively, and A. bisporus larvae were detected mainly in the soil. The control effects after once spraying S. carpocapsae at $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ was higher at the 14th day than at the 7th day. Therefore, we investigated the control effect after twice spraying of $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ at the 14th day. The control effects after this spraying were 51.9% and 96.8%, respectively, and the control effect of spraying at $2.5{\times}10^5m^{-2}$ was better than at $6{\times}10^4m^{-2}$. The control effect of diflubenzuron WP was lower than 50%.

Evolutionary Algorithm for solving Optimum Communication Spanning Tree Problem (최적 통신 걸침 나무 문제를 해결하기 위한 진화 알고리즘)

  • Soak Sang-Moon;Chang Seok-Cheol;Byun Sung-Cheal;Ahn Byung-Ha
    • Journal of KIISE:Software and Applications
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    • v.32 no.4
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    • pp.268-276
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    • 2005
  • This paper deals with optimum communication spanning tree(OCST) problem. Generally, OCST problem is known as NP-hard problem and recently, it is reveled as MAX SNP hard by Papadimitriou and Yannakakis. Nevertheless, many researchers have used polynomial approximation algorithm for solving this problem. This paper uses evolutionary algorithm. Especially, when an evolutionary algorithm is applied to tree network problem such as the OCST problem, representation and genetic operator should be considered simultaneously because they affect greatly the performance of algorithm. So, we introduce a new representation method to improve the weakness of previous representation which is proposed for solving the degree constrained minimum spanning tree problem. And we also propose a new decoding method to generate a reliable tree using the proposed representation. And then, for finding a suitable genetic operator which works well on the proposed representation, we tested three kinds of genetic operators using the information of network or the genetic information of parents. Consequently, we could confirm that the proposed method gives better results than the previous methods.

Effect of STAT3 on Lysophosphatidic Acid-Induced Oral Cancer Cell Invasion

  • Song, Zi Hae;Cho, Kyung Hwa;Kim, Jin Young;Lee, Hoi Young
    • Journal of dental hygiene science
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    • v.19 no.2
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    • pp.141-146
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    • 2019
  • Background: Oral cancer has a high incidence worldwide and has been closely associated with smoking, alcohol, and infection by the human papillomavirus. Metastasis is highly important for oral cancer survival. Lysophosphatidic acid (LPA) is a bioactive lipid mediator that promotes various cellular processes, including cell survival, proliferation, metastasis, and invasion. Signal transducer and activator of transcription (STATs) are transcription factors that mediate gene expression. Among the seven types of STATs in mammals, STAT3 is involved in invasion and metastasis of numerous tumors. However, little is known about the role of STAT3 in oral tumor invasion. In the present study, we hypothesized that STAT3 mediates LPA-induced oral cancer invasion. Methods: Immunoblotting was performed to analyze LPA-induced STAT3 activation. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed to assess the survival rates of YD-10B cells. STAT3 levels in LPA-treated oral tumor cells were evaluated by performing in vitro invasion assay. Results: To the best of our knowledge, this is the first study to demonstrate that LPA enhances STAT3 phosphorylation in oral cancer. In addition, treatment with WP1066, a selective inhibitor of STAT3, at a concentration that does not cause severe reduction in cell viability, significantly attenuated LPA-induced YD-10B cancer cell invasion. Conclusion: The results suggested that LPA induces oral tumor cells with greater invasive potential via STAT3 activation. Our findings provided important insights into the mechanisms underlying mouth neoplasms.

Effect of Various Packaging Methods on Small-Scale Hanwoo (Korean Native Cattle) during Refrigerated Storage

  • Yu, Hwan Hee;Song, Myung Wook;Kim, Tae-Kyung;Choi, Yun-Sang;Cho, Gyu Yong;Lee, Na-Kyoung;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.38 no.2
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    • pp.338-349
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    • 2018
  • The objective of this study was to investigate comparison of physicochemical, microbiological, and sensory characteristics of Hanwoo eye of round by various packaging methods [wrapped packaging (WP), modified atmosphere packaging (MAP), vacuum packaging (VP) with three different vacuum films, and vacuum skin packaging (VSP)] at a small scale. Packaged Hanwoo beef samples were stored in refrigerated conditions ($4{\pm}1^{\circ}C$) for 28 days. Packaged beef was sampled on days 0, 7, 14, 21, and 28. Physicochemical [pH, surface color, thiobarbituric acid reactive substances (TBARS), and volatile basic nitrogen (VBN) values], microbiological, and sensory analysis of packaged beef samples were performed. VP and VSP samples showed low TBARS and VBN values, and pH and surface color did not change substantially during the 28-day period. For VSP, total viable bacteria, psychrotrophic bacteria, lactic acid bacteria, and coliform counts were lower than those for other packaging systems. Salmonella spp. and Escherichia coli O157:H7 were not detected in any packaged beef samples. A sensory analysis showed that the scores for appearance, flavor, color, and overall acceptability did not change significantly until day 7. In total, VSP was effective with respect to significantly higher $a^*$ values, physicochemical stability, and microbial safety in Hanwoo packaging (p<0.05).

The Evaluating Metod of the Insecticidal Activity of Three Chitin Synthesis Inhibitors against the Yellow Mealworm, Tenebrio molitor Linnaeus (갈색거저리(Tenebrio molitor L.)에 대한 키틴합성저해제의 활성평가법)

  • Park, No-Joong;Song, Cheol;Kim, Gil-Hah;Cho, Kwang-Yun
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.281-285
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    • 1994
  • This study was conducted to establish a nce insect pest control system, ~ es.ys tematic application of insecticides using carbohran and buproiezin, in Korea. The effects oi various dates of application and rates of buprofezin (25% WP) after carbofuran (3G) soil incorpombon in late May on the pop~~lation densities of the brown planthoppa (BPH) immigrating in July were investigated Appropriate application tune of buprofezin for the BPH that had evaded insecticidal effect of 5011 incorporated carbofuran was late July-early August. Application rate of buprofezln at 7.0g a.i/lOa was enough to suppress the BPH density. Buprofezin treatment after carbofumn soil incorporation could also suppress the whlte backed planthopper population but did not affect the densities oi the paddy rice spiders. Considering the charactenstics of occurring patterns of the nce insect pests in Korea. buprofezin treatment m late July or early August after carbofuran soil incorporation in late May can be a useful application system of ir~sectic~deins controlling early season Insect pests and migmtoly planthoppers on rice.

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Long term trends in the Korean professional baseball (한국프로야구 기록들의 장기추세)

  • Lee, Jang Taek
    • Journal of the Korean Data and Information Science Society
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    • v.26 no.1
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    • pp.1-10
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    • 2015
  • This paper offers some long term perspective on what has been happening to some baseball statistics for Korean professional baseball. The data used are league summaries by year over the period 1982-2013. For the baseball statistics, statistically significant positive correlations (p < 0.01) were found for doubles (2B), runs batted in (RBI), bases on balls (BB), strike outs (SO), grounded into double play (GIDP), hit by pitch (HBP), on base percentage (OBP), OPS, earned run average (ERA), wild pitches (WP) and walks plus hits divided by innings pitched (WHIP) increased with year. There was a statistically significant decreasing trend in the correlations for triples (3B), caught stealing (CS), errors (E), completed games (CG), shutouts (SHO) and balks (BK) with year (trend p < 0.01). The ARIMA model of Box-Jenkins is applied to find a model to forecast future baseball measures. Univariate time series results suggest that simple lag-1 models fit some baseball measures quite well. In conclusion, the single most important change in Korean professional baseball is the overall incidence of completed games (CG) downward. Also the decrease of strike outs (SO) is very remarkable.

Genotoxicity Study of HM10411, Recombinant Human Granulocyte Colony Stimulating Factor (재조합 인과립구 콜로니 자극인자 HM10411의 유전독성 연구)

  • 권정;이미가엘;홍미영;조지희;정문구;권세창;이관순
    • Biomolecules & Therapeutics
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    • v.10 no.4
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    • pp.268-273
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    • 2002
  • Mutagenic potential of HM10411 (recombinant human granulocyte colony stimulating factor) was evaluated by bacterial reverse mutation test, in vitro chromosome aberration test and in vivo micronucleus test. The bacterial reverse mutation test was performed using the histidine auxotroph strains of Salmonella typhimurium TA100, TA1535, TA98, TA1537 and tryptophan auxotroph strain of Escherichia coli WP2 uvrA. The negative results of the bacterial reverse mutation test suggest that HM10411 does not induce mutation, in the genome of Salmonella typhimurium and E. coli under the conditions used. In addition, it has little clastogenicity either in vitro chromosome aberration test or in vivo micronucleus test. For in vitro chromosomal aberration test, Chinese hamster lung(CHL) cells were exposed to HM10411 of 23, 46 or 92 $\mu\textrm{g}$/ml for 6 or 24 hours in the absence and for 6 hours in the presence of metabolic activation system. There was no significant increase in the number of aberrant metaphase in HM 10411-treated groups at any dose levels both in the presence and absence of metabolic activation system. The micronucleus test was carried out using specific pathogen free(SPF) 7-week old male ICR mice, The test item, HM10411 was intraperitoneally administered at 1150, 2300 or 4600 $\mu\textrm{g}$/kg once a day for 2 consecutive days. There was no significant increase in the frequencies of micronucleated polychromatic erythrocytes(PCEs) at any treated groups compared with negative control group. Therefore, these results demonstrate that the test item, HM10411, was not mutagenic under the condition of these studies.

Survey the Occurrences and Establishment of Environment-friendly Control System of Ricania shanthungensis in Jeonnam Province (전남지역 갈색날개매미충 발생현황과 친환경 방제)

  • Choi, Duck-Soo;Ma, Kyung-Cheol;Kim, Hyo-Jeong;Lee, Jin-Hee;Oh, Sang-A;Kim, Seon-Gon
    • Korean Journal of Organic Agriculture
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    • v.26 no.3
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    • pp.439-452
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    • 2018
  • This study was carried out to establish of environment-friendly control system and to survey the occurrence status of Ricania shanthungensis in Jeonnam province from 2016 to 2017. R. shanthungensis occurred at 1,344 ha in 61 towns of 15 cities in Gwangju and Jeonnam province. At four sites in the stationary area, the densities of eggmass and pupae were lower in 2017 than in 2016, the hatching rate decreased, and the first hatching time, emergence of adults, and the start of spawning tended to be slower. Telenomus sp., an egg parasitoid of R. shanthungensis, was found in most areas. Sunchon had the highest rate of 46.1%, followed by Muan 40.6, Goksung 29.2, Gurye 25.8, and Gwangju 17, respectively. The optimum spraying time for the control of R. shanthungensis was early March. The spraying material and the dilution multiples are 10 times of the machine oil at environmental friendly cultivation and 500 times of the chlorpriphosphate wp. at normal cultivation. When sprayed these materials, 95% of egg prohibited to hatch. There was more than 80% of the insecticidal effects on the organic materials of the machine oil emulsion, the Sophora root extrect + microbial extract agent, and the castor emulsion. For adults, Sophora root extrect + microbial extract, neem extract, and machine oil were better. Four kinds of chemical pesticides such as dinotefuran wp were effective for nymph and adult control. We have developed an adult catching device using the most preferred daylight color and behavioral habits of R. shanthungensis. The capture device consisted of two daylight compact lamps (30W and 20W), a yellow plate, and a catcher using water, and caught about 700 individuals a day. Based on the above results, we have established a system for controlling and life cycle of R. shanthungensis in Jeonnam province.

Micro-Hydropower System with a Semi-Kaplan Turbine for Sewage Treatment Plant Application: Kiheung Respia Case Study (하수처리장 적용을 위한 Semi-카플란 수차가 장착된 마이크로수력발전 시스템: 기흥레스피아 사례)

  • Chae, Kyu-Jung;Kim, Dong-Soo;Cheon, Kyung-Ho;Kim, Won-Kyoung;Kim, Jung-Yeon;Lee, Chul-Hyung;Park, Wan-Soon
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.5
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    • pp.363-370
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    • 2013
  • Small scale hydropower is one of most attractive and cost-effective energy technologies for installation within sewage treatment plants. This study was conducted to evaluate the potential of a semi-kaplan micro-hydropower (MHP) system for application to sewage treatment plants with high flow fluctuations and a low head. The semi-kaplan MHP is equipped with an adjustable runner blade, and is without a guide vane, so as to reduce the incidence of mechanical problems. A MHP rating 13.4 kWp with a semi-kaplan turbine has been considered for Kiheung Respia sewage treatment plant, and this installation is estimated to generate 86.8 MWh of electricity annually, which is enough to supply electricity to over 25 households, and equivalent to an annual reduction of 49 ton $CO_2$. The semi-kaplan turbine showed a 90.2% energy conversion efficiency at the design flow rate of 0.35 $m^3/s$ and net head of 4.7 m, and was adaptable to a wide range of flow fluctuations. Through the MHP operation, approximately 2.1% of total electricity demand of Kiheung Respia sewage treatment plant will be achievable. Based on financial analysis, an exploiting MHP is considered economically acceptable with an internal rate of return of 6.1%, net present value of 15,539,000 Korean Won, benefit-cost ratio of 1.08, and payback year of 15.5, respectively, if initial investment cost is 200,000,000 Korean Won.

Lodging Related Traits of Rice Plants as Affected by ′KIM -112′ Application (KIM-112 처리가 벼 도복관련형질에 미치는 영향)

  • Choi, Chung-Don;Kim, Soon-Chul;Lee, Soo-Kwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.3
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    • pp.218-223
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    • 1990
  • This experiment was carried out to obtain basic information on the lodging characteristics and yield components of rice plants by new anti-lodging reagent 'KIM-112' (3%, Wp) application at 30 days before heading (DBH) to 5 DBH under two dosage levels (lg 2g, a. i. /10a) at the Yeongnam Crop Experiment Station in 1989. Culm length was shortened by 10-17% at 1g, a. i. /10a and by 16-23% at 2g of KIM -112 applications. The shortening effect of internode was different by dosages and application times: 2g treatment was greater effect than 19 and early application resulted in shortening of lower internode while this was upper internode at the late application. There were positive correlation between culm length and lodging index, the 1st and the 4th internode lengths had on important effect to lodging index. Thickness of culm wall, culm diameter and weight of basal part of culm were not affected by KIM-112 application. However anti-lodging characters improved by increasing the breaking weight and by decreasing the moment. Lodging index was related to breaking weight, moment and weight of panicle. Heading date by KIM-112 application was delayed one to four days and number of grains per panicle was decreased by shortened panicle length as compared with untreated control, but yield capacity was high a little because of improvement ripening ratio by no lodging.

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