• Biomolecules & Therapeutics
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• 제30권1호
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• pp.72-79
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• 2022

Licochalcone H (LCH) is a phenolic compound synthetically derived from licochalcone C (LCC) that exerts anticancer activity. In this study, we investigated the anticancer activity of LCH in human skin cancer A375 and A431 cells. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay was used to evaluate the antiproliferative activity of LCH. Cell cycle distribution and the induction of apoptosis were analyzed by flow cytometry. Western blotting assays were performed to detect the levels of proteins involved in cell cycle progression, apoptosis, and the JAK2/STAT3 signaling pathway. LCH inhibited the growth of cells in dose- and time-dependent manners. The annexin V/propidium iodide double staining assay revealed that LCH induced apoptosis, and the LCH-induced apoptosis was accompanied by cell cycle arrest in the G1 phase. Western blot analysis showed that the phosphorylation of JAK2 and STAT3 was decreased by treatment with LCH. The inhibition of the JAK2/STAT3 signaling pathway by pharmacological inhibitors against JAK2/STAT3 (cryptotanshinone (CTS) and S3I-201) simulated the antiproliferative effect of LCH suggesting that LCH induced apoptosis by modulating JAK2/STAT3 signaling.

• 한국사회복지학
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• 제62권2호
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• pp.375-397
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• 2010

임파워먼트와 관련된 여러 선행연구들은 현재의 실천이 임파워먼트의 실제 이념을 충분히 반영하지 못한 채 매우 미시적이고 제한적으로만 이해되고 있음을 공통적으로 지적하고 있다. 또 이러한 실천을 가능하게 하는 구체적 기법에 대한 정보가 부족함을 강조하고 있다. 이에 본 연구에서는 구조적이고 제도적인 변화를 경험하고 있으며, 이러한 활동의 결과로 실제적인 정책변화를 이루어낸 사례로서 장애인 자립생활운동과 그 발전과정에 나타난 임파워먼트의 특성을 파악하고자 하였다. 분석결과 임파워먼트의 과정은 총 5단계로 구성되며 그 중 개인의 임파워먼트와 관련해서는 의식화 과정이, 거시적 임파워먼트와 관련해서는 조직화가 매우 중요한 과제임을 알 수 있었다.

• 생약학회지
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• 제45권4호
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• pp.366-375
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• 2014

Aldose reductase (AR) has been indicated the critical enzyme of the polyol pathway in the development of the diabetic complications. In this study, 119 Vietnamese herbal medicines have been investigated for inhibitory activities on AR. Among them, 7 herbal medicines, Synedrella nodiflora (stems and leaves), Combretum sundaicum (stems and leaves), Argyreia acuta (stems and leaves), Platea latifolia (whole plants), Linociera sangda (whole plants), Rhaphiolepis indica (whole plants), Harrisonia perforata (stems and leaves) and 2 fractions from Harrisonia perforata (stems and leaves) with EtOAc and BuOH, exhibited a significant inhibitory activity against AR. Particularly, S. nodiflora, A. acuta and H. perforata (stems and leaves) showed 1.0-1.8 times more potent inhibitory activity than the positive control, 3,3-tetramethyleneglutaric acid (TMG).

• International Journal of Concrete Structures and Materials
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• 제11권2호
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• pp.365-375
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• 2017

An experimental evaluation of crack propagation and post-cracking behavior in steel fiber reinforced concrete (SFRC) beams, using full-field displacements obtained from the digital image correlation technique is presented. Surface displacements and strains during the fracture test of notched SFRC beams with volume fractions ($V_f$) of steel fibers equal to 0.5 and 0.75% are analyzed. An analysis procedure for determining the crack opening width over the depth of the beam during crack propagation in the flexure test is presented. The crack opening width is established as a function of the crack tip opening displacement and the residual flexural strength of SFRC beams. The softening in the post-peak load response is associated with the rapid surface crack propagation for small increases in crack tip opening displacement. The load recovery in the flexural response of SFRC is associated with a hinge-type behavior in the beam. For the stress gradient produced by flexure, the hinge is established before load recovery is initiated. The resistance provided by the fibers to the opening of the hinge produces the load recovery in the flexural response.

• 지역사회간호학회지
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• 제17권3호
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• pp.364-375
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• 2006

Purpose: The purpose of the study was to investigate burden and the qualify of life in caregivers who are taking care of home-based rare and incurable disease patients. Methods: The subjects of this study were 300 caregivers of rare and incurable disease patients registered at five health centers in Seoul. A survey was conducted by mail and visit in person during the period from the 25th of March to the 12th of May 2005. Collected data were analyzed through t-test, ANOVA, Pearson's correlation coefficient. Result: The mean burden of caregivers was 3.42, and the mean qualify of life of caregivers was 2.71. Burden and QoL showed significant differences according to caregivers' characteristics such as sender, age, relation to the patient, academic qualification, religion, occupation, monthly household income and perceived health condition. Caregivers' burden was in an inverse correlation with their quality of life. Conclusions: According to the results of this study, rare and incurable disease caregivers' burden and their quality of life were in a significant correlation with each other. In order to improve caregivers' quality of life by reducing their burden, we need to reestablish comprehensive policies for rare and incurable disease management including nursing intervention strategies for caregivers.

• 보건행정학회지
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• 제33권3호
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• pp.370-375
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• 2023

The responsibility to ensure the health rights of detainees, particularly their medical rights, fundamentally lies with the state in all nations. However, in the correctional facilities of the Republic of Korea, these rights are currently not adequately safeguarded. Numerous detainees express dissatisfaction with the medical services provided and show a preference for voluntary external treatment. However, barriers such as prolonged application processes for external treatment and the requirement for detainees to cover their medical expenses present significant challenges. Therefore, the National Human Rights Commission of Korea has advocated for an increased medical budget in correctional facilities and a bolstered professional medical workforce to improve the medical care of detainees. Recommendations for improvements include: (1) establishing dedicated correctional hospitals for detainees, (2) setting up specialized correctional wards, (3) collaborating with military hospitals, (4) launching mobile medical buses for diverse specialties, (5) enhancing collaboration with public and private medical institutions, (6) increasing compensation for partnering external medical institutions, (7) improving the working conditions of medical officers, (8) safeguarding the defense rights of medical staff, (9) improving the working conditions of public health doctors from the Ministry of Justice in correctional facilities, and (10) pre-assigning public health specialists and military doctors to correctional facilities. By implementing these measures, it is anticipated that the quality of medical services in the Republic of Korea's correctional facilities will improve, reducing the demand for external treatments among detainees and ensuring their health and medical rights are realistically upheld.

• 정보처리학회논문지B
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• 제9B권3호
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• pp.375-382
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• 2002

필기체 문자 인식은 온라인 필기체 문자 인식과 오프라인 필기체 문자 인식으로 나누어진다. 온라인 필기체 문자 인식은 타블렛과 같은 펜 기반의 전자식 입력 장치를 이용하여 필기의 순서와 획의 위치와 같은 동적인 필기 정보를 문자의 입력 시 획득할 수 있어 오프라인 필기체 문자 인식에 비해 큰 연구 성과를 이루었다. 그러나 오프라인 필기체 문자 인식은 온라인 필기체 문자 인식에서와 같이 동적인 정보를 입력받을 수 없고, 다양한 필기와 자소의 겹침이 심하며 획 사이의 잡영을 많이 가지고 있어 인식의 전처리 결과에 따라 인식 성능이 크게 달라진다. 본 논문에서는 오프라인 필기체 한글 문자 인식을 위해 문자의 동적인 정보를 포함하는 획을 효과적으로 추출하는 방법을 제안한다. 제안된 방법은 전처리 과정으로 먼저 Watershed 알고리즘을 이용하여 입력된 필기체 문자 영상의 향상 및 이진화를 수행한다. 이진화된 문자부를 변형된 Lu와 Wang의 세선화 알고리즘을 사용하여 세선화를 수행한 후 문자에서의 특징점을 추출하여 세그먼트 화소열을 추출하고, 최대 허용 오차법을 이용하여 벡터화한다. 벡터화의 수행으로 몇 개의 획이 하나의 세그먼트로 묶인 경우, 하나의 세그먼트 화소열은 2 또는 그 이상의 세그먼트 벡터로 분리된다. 추출된 세그먼트 벡터들을 완전한 획으로 재구성하기 위해서 오른손 필기 좌표계 시스템을 이용하여 벡터의 방향적인 성분을 인간의 필기 획의 방향에 알맞게 수정하고, 수정된 세그먼트 벡터의 방향성과 분기 정보를 이용하여 인접한 결합 가능한 세그먼트 벡터를 결합함으로써 문자 인식에 적합한 완전한 획으로 재구성한다. 실험 결과 제안된 방법이 필기체 한글 문자 인식에 적합함을 알 수 있었다.

• 한국약용작물학회지
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• 제27권6호
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• pp.365-375
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• 2019

Background: Oxidative stress plays an important role in neuro-degenerative disorders such as Alzheimer's disease. Oxidative stress is mediated by reactive oxygen species (ROS), which are implicated in the pathogenesis of numerous diseases, and account for the toxicity of a wide range of compounds. Methods and Results: In order to study the neuro-protective effect of the complex extracts of Aster scaber Thunberg (AS) and Chaenoleles sinensis Koehne (CSK) against hydrogen peroxide in PC12 cells, cell viability was evaluated by the MTT assay using tetrazole, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and the intracellular ROS levels were determined the by 2',7'-dichlorofluorescein diacetate (DCF-DA) assay. In order to examine the anti-amnesic effects of the complex extracts of AS and CSK, behavioral tests were performed on male ICR mice. The ameliorating effect of the complex extracts against Aβ_1-42-induced learning and memory impairment was analyzed by y-maze and passive avoidance tests. The AS and CSK extracts showed neuro-protective activity both in vitro and in vivo, and the neuro-protective effect of their 60 : 40 (AS : CSK) mixture was better than that of the other mixtures. Moreover, the complex extracts synergistically inhibited acetylcholinesterase activity and rapid peroxidation. Conclusions: A mixture of the AS and CSK extracts could be used to develop functional foods and serve as raw materials for the development of therapeutics against Alzheimer's disease.

• Toxicological Research
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• 제26권2호
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• pp.101-108
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• 2010

Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 mg/kg in corn oil once for dose response or treated orally with 1-BB at 1500 mg/kg for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.

• Parasites, Hosts and Diseases
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• 제36권1호
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• pp.15-22
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• 1998

Strongyloides venezuelensis을 자유생활형 유충을 얻고자, 쥐의 분변 물질을 첨가한 영양 배지를 polyvinyl bag에 넣어 배양하는 배양기술을 확립하였다 포화식염수법으로 회수한 충란의 부화율은 배양 온도에 따라서 다르게 관찰되었는데 (Y = $-0.192X^2$ + 8.673x - 19.550, r = 0.901) $^25{\circ}C$에서 100% 부화율을 나타내었지만 온도가 낮거나 높아도 감소되는 양상을 나타내었다. 회수 한 충란 $^20{\circ}C$에서 5일간 관찰하였을 때 자유생활형 유충으로의 발육률은 영양 배지의 농도를 증가시키면 증가하여 0 12%에서 최고치를 보이다가 다시 낮아지는 양상을 보여 (Y = $-864.032X^2$ + 245.995X - 0.560, r = 0.875), 감염형 3기 유충($L_3$)으로의 발육률은 배양 온도에 따라서 다르게 관찰되었다 즉, $^25{\circ}C$에서 가장 높아서 20%에 달하며 $15^{\circ}C$는 10.9%, 그리고 $^20{\circ}C$에서는 18.1%로 나타났다 (Y = $-0.189X^2$ + 8.387x - 72 795 r = 0.981). 그렇지만 감염 유충으로 성장하는데 필요한 기간도 역시 온도의 변화와 관련이 있어 온도가 높으 면 높을수록 소요되는 시간이 잔게 나타났다 (Y = $0.035X^2$ - 2.025X + 32.375, r = 0.995) 이상의 결과로 미루어 보아 감염 유충의 최대 회수율 (19.2%)은 15,000개를 polyvinyl bag에 0.12% 영양 배지와 더불어 $^25{\circ}C$에서 5일간 배양하였을 때 얻을 수 있었다. 이처럼 새로이 고안한 Skonavloides 속의 기생충 배양 기술은 화학 물질, 구충제, 제초제에 대한 선별검사와 기생충과 관련된 면역학적 연구에 생물학적 도구로서 사용될 수 있을 것으로 기대된다. 저자는 S. venezuelensis의 국문 이름을 베네수엘라분선충으로 제안한다.