• Molecules and Cells
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• 제28권4호
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• pp.375-382
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• 2009

The 26S proteasome is a 2-MDa complex with a central role in protein turn over. The 26S proteasome is comprised of one 20S core particle and two 19S regulatory particles (RPs). The RPN12a protein, a non-ATPase subunit of the 19S RP, was previously shown to be involved in cytokinin signaling in Arabidopsis. To further investigate cellular roles of RPN12a, RNAi transgenic plants of RPN12a were constructed. As expected, the 35S:RNAi-RPN12a plants showed cytokinin signaling defective phenotypes, including abnormal formation of leaves and inflorescences. Furthermore, RNAi knock-down transgenic plants exhibited additional unique phenotypes, including concave and heart-shape cotyledons, triple cotyledons, irregular and clustered guard cells, and defects in phyllotaxy, all of which are typical for defective cytokinin signaling. We next examined the mRNA level of cytokinin signaling components, including type-A ARRs, type-B ARRs, and CRFs. The expression of type-A ARRs, encoding negative regulators of cytokinin signaling, was markedly reduced in 35S:RNAi-RPN12a transgenic plants relative to that in wild type plants, while type-B ARRs and CRFs were unaffected. Our results also indicate that in vivo stability of the ARR5 protein, a negative regulator of cytokinin signaling, is mediated by the 26S proteasome complex. These results suggest that RPN12a participates in feedback inhibitory mechanism of cytokinin signaling through modulation of the abundance of ARR5 protein in Arabidopsis.

• Archives of Pharmacal Research
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• 제29권2호
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• pp.135-139
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• 2006

Activity-guided fractionation of the ethanol extract of the whole plant from Solanum lyratum resulted in the isolation of a new pregnane derivative glycoside, 16-dehydropregnenolone 3-O-${\alpha}$-L-rhamnopyranosyl-($1{\to}2$)-${\beta}$-D-glucopyranosid uronic acid (2), as well as other six known compounds: 16-dehydropregnenolone (1), allopregenolone (3), protocatechuic acid (4), vanillic acid (5), caffeic acid (6), and scopoletin (7). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compounds 1, 3, 4 were isolated for the first time from this plant. Cytotoxic activities of the isolated compounds were evaluated. Compound 1 exhibited significant cytotoxic activity against A375-S2, HeLa, SGC-7901, and Bel-7402 with $IC_{50}$ values of $13.1{\pm}0.9,\;21.5{\pm}1.0,\;40.2{\pm}0.7$, and $49.8{\pm}1.2\;{\mu}g/mL$, respectively.

• 생명과학회지
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• 제13권4호
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• pp.375-382
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• 2003

본 연구는 콩 cyst 선충 race 14에 대한 저항성 QTLs 구명을 목적으로 한 바 결과를 요약하면 다음과 같다. 1. 회귀분석 결과 30개의 marker들(29 RAPD, 1 RFLP)에서 cyst 선충 race 14의 저항성에 대한 유의성이 인정되었다. 2. MAPMAKER/QTL 분석 결과 2개의 QTL들이 구명되었는데, 이 QTL들은 2개의 linkage groups (LGC-7와 LGC-9)에 위치하였으며, 모두 우성유전 양상을 나타내었다. 3. 다중회귀분석 결과 2개의 marker들($B15^2$와 $H06^1$)로 구성된 조합에서 가장 높은 표현적 변이의 값(22.9%)을 나타내었다. 콩 cyst 선충 rare 14에 대한 표현적 변이를 충분히 설명하기 위해서는 지속적인 QTL 구명 연구가 요구된다.

• 한국환경복원기술학회지
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• 제11권6호
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• pp.104-111
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• 2008

Plant growth and variegated leaf color changes of three leaf variegated Sedum spectabile varieties 'Variegatum', 'Frosty Morn', red colored 'Washfield Ruby', and one Sedum lineare variety 'Variegatum' and Sedum sarmentosum under three levels of shading conditions were examined for leaf ornamental value improvement. Shading levels were 40%($1,500{\mu}mol{\cdot}m-^2{\cdot}s-^1$), 70%($750{\mu}mol{\cdot}m-^2{\cdot}s-^1$) and 85%($375{\mu}mol{\cdot}m-^2{\cdot}s-^1$). Improvements of leaf ornamental value of 'Variegatum' 'Frosty Morn' and 'Washfield Ruby' were observed at 40% shading level and plant heights and leaf sizes were also increased. Plant height of S.sarmentosum were shorten under direct sun light condition and leaf color changed to greenish yellow(RHS143B) but deep green color(RHS144A) was observed under shading conditions. Endurance of S.sarmentosum was stronger than other species and leaf ornamental values remained well at 70% shading level. Leaf width of S.lineare 'Variegatum' were not changed under shading conditions but leaf length was increased. Leaf color was Deep green color was greenish yellow under direct sun light but deepest green color was observed at 85% shading level. Consider all ornamental value criteria, best shading conditions for S.lineare 'Variegatum' were ranged 40% to 70%.

• 약학회지
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• 제47권6호
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• pp.369-375
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• 2003

Xiaoshuan Zaizao Wan (XZW) has been used in China to improve hemiplegia, deviation of eye and mouth, and dysphasia due to cerebral thrombosis. To characterize pharmacological actions of XZW, we evaluated its effects on neuronal cell damage induced in primary cultured rat cortical cells by various oxidative insults, glutamate or N-methyl-D-aspartate (NMDA), and $\beta$-amyloid fragment ($A_{\beta(25-35)}$). XZW was found to inhibit the oxidative neuronal damage induced by $H_2O_2$, xanthine/xanthine oxidase, or $Fe^{2+}$/ascorbic acid. It also attenuated the excitotoxic damage induced by glutamate or NMDA. The NMDA-induced neurotoxicity was more effectively inhibited than the glutamate-induced toxicity. In addition, we found that XZW protected neurons against the $A_{\beta(25-35)}$-induced toxicity. Moreover; XZW exhibited dramatic inhibition of lipid peroxidation in rat brain homogenates and mild 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Taken together; these results demonstrate that XZW exerts neuroprotective effects against oxidative, excitotoxic, or $A_{\beta(25-35)}$-induced neuronal damage. These findings may provide pharmacological basis for its clinical usage treating the sequelae caused by cerebral thrombosis. Furthermore, XZW may exert beneficial effects on Alzheimer's disease and other oxidative stress-related neurodegenerative disorders.

• Child Health Nursing Research
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• 제26권3호
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• pp.366-375
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• 2020

Purpose: This study was conducted to evaluate the efficacy of a community-based follow-up program on parenting stress, parenting efficacy, and coping among parents with premature infants. Methods: A non-equivalent control group pre-post quasi-experimental design was used. This program consisted of structured home visits and self-help group meetings for 6 months. The experimental group (n=29) received visits by an experienced neonatal intensive care unit (NICU) nurse and the control group (n=27) was visited by a visiting nurse. Data were analyzed using the χ² test, t-test, and analysis of covariance. Results: Parents' coping behavior significantly differed in the experimental group compared to the control group (t=3.14, p=.003). In particular, coping subscale I, for maintaining the family situation (t=2.63, p=.011), and subscale III, for understanding the infant's medical situation (t=4.30, p<.001), showed significant differences in the experimental group. There were no significant between-group differences in parenting stress or parenting efficacy. Conclusion: The findings of this study suggest that home visits by an experienced NICU nurse provided through a community-based follow-up program were an effective intervention to improve coping behavior among parents with premature infants.

• Journal of the Korean Data and Information Science Society
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• 제20권2호
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• pp.369-375
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• 2009

오는 2010년에는 제5회 전국동시지방선거가 실시된다. 본 연구는 이에 대한 준비의 일환으로, 2006년 5월 31일 실시된 제4회 전국동시지방선거자료(전라북도도지사 선거)를 대상으로 소지역주의가 나타났는지 알아보고자 한다. 이를 위하여 지사에 출마한 각 후보자가 지역별로 얼마나 골고루 득표했는지를 측정해 볼 수 있는 RS 지수, 후보자별 지역친밀도를 측정해볼 수 있는 카이통계량, 대응분석 등의 분석방법을 활용하였다. 그 결과 일부 후보에 대하여 지역주의가 심하게 나타나고 있음을 확인하였으며, RS 지수는 종합지수인 관계로 지역주의여부를 평가하는 측도로서의 타당도는 떨어짐을 알 수 있었다.

• 한국IT서비스학회:학술대회논문집
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• 한국IT서비스학회 2008년도 추계학술대회
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• pp.375-378
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• 2008

본 연구에서는 발주자 입장에서 PMI(Project Management Institute)에서 정의하고 있는 프로젝트 범위관리 활동을 범위의 기획 및 정의, 범위의 검증, 범위의 통제 3가지로 구분하고, 프로젝트의 성과를 프로젝트 과정에 대한 성과와 제품에 대한 성과 2가지로 구분하여, 각각의 범위관리 활동들이 각각의 프로젝트 성과에 어떠한 영향을 미치고 있는지에 대한 실증연구를 진행 하였다. 이와 동시에 프로젝트의 규모, 공급자 유형, 경험여부와 같은 프로젝트 특성이 범위관리 활동과 프로젝트 성과 사이의 관계에 어떠한 영향을 미치는지에 대한 연구도 병행하였다. 본 연구의 의의는 발주자 입장에서 범위관리 각각의 활동이 정보시스템 개발 프로젝트의 성과에 어떠한 영향을 미치는지에 대해 실증 연구를 함으로서 정보시스템 개발 프로젝트의 발주자들로 하여금 프로젝트에 있어 범위관리의 각각의 활동들에 대한 중요도를 인식시켜 프로젝트 범위관리 활동에 대한 적극적인 참여와 관심을 고취시키는데 그 의의가 있다.

• 동의생리병리학회지
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• 제18권2호
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• pp.371-375
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• 2004

The eyes can register color oscillations and that colors have an effect on the psyche. Human is constantly surrounded by an immense range of vibrations to which he must react and respound. And the skin can be compared to an antenna which amplifies information received from the outside and then passes it on to the inside. Therefore the art of many medicines make use of the skin as a medium of treatment. Peter mandel discovered centers, or zones, on the human body, which represent or correspond to the three primary colors(red, blue and yellow). And he came across to the four temperaments(or humors) and their respective colors, which consist of the primary colors with the addition of green.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2001년도 추계학술대회(제116회) 및 13회 Workshop 제3회 한ㆍ일 치과보존학회 공동학술대회 초록집
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• pp.589.1-589
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• 2001

The aim of this study is to evaluate the effect of light intensity variation on the polymerization rate of composite resin using IB system(experimental equipment designed by Dr. IB Lee) by which real-time volumetric change of composite can be measured. Methods: Three commercial composites [Z100(Z1), AeliteFil(AF), SureFil(SF)] were photopolymerized with Variable Intensity Polymerizer unit (Bisco, U.S.A.) under the variable light intensity (75/150/225/300/375/450㎽$^2$) during 20sec.(omitted)