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A Study on Listeria Strain Species for Fishes and Shellfishes on Sale (시판되는 어 .패류에 대한 Listeria 속균의 조사연구)

  • 김동필;조배식
    • The Korean Journal of Food And Nutrition
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    • v.14 no.6
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    • pp.548-561
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    • 2001
  • Listeria spp. from sea water, fishes and shellfishes have been troubled in many countries. So we exam ined its distribution rates, biochemical characteristics of a separated strain, growth curve of pH at set times to 4 species of standard strain, and yes or no of growth inhibition for precautionary measure of food poisoning by L. monocytogenes, garlic, mustard, wasabi, and green tea extracts including sensitivity of antibiotics 10 species. As its results, check numbers of its positivity to Listeria spp. were 32 species in total examination body 200 species, and its isolation rates were 16%, L. innocua was 14.0%, L. monocytogenes 1.0%, and L. seeligeri 1.0% by the strain species. All the standard strain of 4 species showed growth inhibition bellow pH 3.0, its pH conditions of the optimum growth at 7.0∼8.0, and its growth was more active in alkali co]tuition than in acid condition. Its growth inhibition examination by garlic extracts had an the worst effects with O.D values of 0.078∼0.210. But the case of mustard and wasabi had weakened effect, and the case of green tea had some effect as the time went by. The results of sensitivity examination of antibiotics 10 species were as fellows. L. innocua of the 16 cases showed sensitivity of 100% in all 5 species, Ampicillin, etc, and Ciprofloxacin showed sensitivity of 43.7% and gentamicin, 93.7%. But tetracycline showed tolerance of 31.3% , cefotaxine. 75%, nalidixic acid, 100%. L. monocytogenes of the 6 cases showed sensitivity of 100% in all 6 species, ciprofloxacin, etc.

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Neurochemical Profile Quantification of Regional Adult Mice Brain Using: ex vivo $^1H$ High-Resolution Magic Angle Spinning NMR Spectroscopy (생체 외 조직 고 분해능 Magic Angle Spinning을 이용한 정상 Adult Mice에서의 뇌 부위별 뇌 신경화학 대사물질 정량분석)

  • Lee, Do-Wan;Woo, Dong-Cheol;Lee, Sung-Ho;Kim, Sang-Young;Kim, Goo-Young;Rhim, Hyang-Shuk;Choi, Chi-Bong;Kim, Hwi-Yool;Lee, Chang-Wook;Choe, Bo-Young
    • Progress in Medical Physics
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    • v.21 no.1
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    • pp.35-41
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    • 2010
  • The purpose of this study is to quantitate regional neurochemical profile of regional normal adult mice brain and assess regional metabolic differences by using ex vivo $^1H$ high-resolution magic angle spinning nuclear magnetic resonance spectroscopy ($^1H$ HR-MAS NMRS). The animals were matched in sex and age. The collected brain tissue included frontal cortex, temporal cortex, thalamus, and hippocampus. Quantitative 1D spectra were acquired on 40 samples with the CPMG pulse sequence (8 kHz spectral window, TR/TE = 5500/2.2 ms, NEX = 128, scan time: 17 min 20 sec). The mass of brain tissue and $D_2O$+TSP solvent were 8~14 mg and 7~13 mg. A total of 16 metabolites were quantified as follow: Acet, NAA, NAAG, tCr, Cr, tCho, Cho, GPC + PC, mIns, Lac, GABA, Glu, Gln, Tau and Ala. As a results, Acet, Cho, NAA, NAAG and mIns were showed significantly different aspects on frontal cortex, hippocampus, temporal cortex and thalamus respectively. The present study demonstrated that absolute metabolite concentrations were significantly different among four brain regions of adult mice. Our finding might be helpful to investigate brain metabolism of neuro-disease in animal model.

An Exploratory Study of REID Benefits for Apparel Retailing (의류소매업에서의 RFID 이점에 대한 탐색적 연구)

  • Kim, Hae-Jung;Kim, Eun-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.12 s.159
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    • pp.1697-1707
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    • 2006
  • Relentless advances in information technology are constantly transforming market dynamics of the retail industry. RFID is an emerging innovative technology that can reduce labor costs, improve inventory control and increase sales by effective business processes. Apparel retailers need to recognize the benefits of RFID and identify critical success factors. By focusing on apparel retailers, this study attempts (1) to identify the reality of RFID associated with benefits; and (2) to prospect the implementation of RFID in apparel retailing. We conducted a focus group interview with selected six panels who were experts of retail industry in the United States to obtain data regarding RFID attributes. Content analysis was used to generate related excerpts and classify 31 attributes of RFID benefits from the meaningful 173 responses. For experience of RFID, retailers were familiar with RFID technology and expressed the belief that RFID basically would support an existing retail system for speed to markets. However, retailers addressed the level of experience with RFID technology that they were still in the early adoption stage among few innovative companies. The content analysis identified five dimensions of RFID benefits for apparel retailing: Visibility and Velocity, Revenue Enhancement, Customer Service, Security, and Employee Productivity. This result lends support to the belief that RFID has a significant potential to streamline supply chain management, store operation and customer service for apparel retailing. This study provides intellectual and managerial implications far practitioners and researchers by postulating the effective use of RFID in the apparel retail industry.

Skarn Formation in Metamorphic Rocks of the Chungju Mine Area (충주광산 지역 계명산층의 텅스텐 스카른화작용)

  • Kim, Gun-Soo;Park, Maeng-Eon
    • Economic and Environmental Geology
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    • v.28 no.3
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    • pp.185-197
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    • 1995
  • Tungsten skarns in the Chungju mine which consists mainly of strata-bound type iron ore deposits are found in the vicinity of the contact between the age-unknown Kyemeongsan Formation and granitic rock intrusions of Mesozoic age($134{\pm}2Ma$). Tungsten skarns were formed extensively from alumina and silica-rich schistose rocks by the introduction of calcium and iron from hydrothermal solution. The skarns comprise a metasomatic column and are subdivided into four facies; garnet facies, wollastonite facies, epidote facies and chlorite facies. The skarn process in time-evolutional trend can be divided broadly into the four facies in terms of the paragenetic sequence of calc-silicates and their chemical composition. Skarn and ore minerals were formed in the following sequence; (1) garnet facies, adjacent to biotite granite, containing mainly garnet(>Ad96) and magnetite, (2) wollastonite facies containing mainly wollastonite and garnet(Ad95~60), (3) epidote facies, containing mainly epidote(Ps35~31), quartz, andradite-grossular(Ad63~50), and scheelite, (4) chlorite facies, adjacent to and replacing schist, containing mainly chrolite, muscovite, quartz, calcite, epidote(Ps31~25), hematite and sulfides. The mineral assemblage and mineral compositions. suggest that the chemical potentials of Ca and Fe increased toward the granitic rock, and the component Al, Mg, K, and Si decreased from the host rock to granitic rock. The homogenization temperature and salinity of fluid inclusion in scheelite, quartz and epidote of epidote facies skarn is $300-400^{\circ}C$ and 3-8wt.% eqiv. NaCl, respectively. ${\delta}^{34}S$ values of pyrite and galena associated with chlorite facies skarn is $9.13{\sim}9.51%_{\circ}$ and $5.85{\sim}5.96%_{\circ}$, respectively. The temperature obtained from isotopic com· position of coexisting pyrite-galena is $283{\pm}20^{\circ}C$. Mineral assemblages and fluid inclusion data indicate that skarn formed at low $X_{CO_2}$, approximately 0.01. Temperature of the skarn mineralization are estimated to be in the range of $400^{\circ}C$ to $260^{\circ}C$ and pressure to be 0.5 kbar. The oxygen fugacity($fo_2$) of the skarn mineralization decreased with time. The early skarn facies would have formed at log $fo_2$ values of about -25 to -27, and late skarn facies would have formed at log $fo_2$ values of -28 to -30. The estimated physicochemical condition during skarn formation suggests that the principal causes of scheelite mineralization are reduction of the ore·forming fluid and a decrease in temperature.

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Effects of Dietary Escherichia coli Phytase Supplementation on Growth Performance, Carcass Quality and Excretion of Copper and Zinc Concentrations in Finishing Pigs (Escherichia coli phytase의 첨가가 비육돈의 생산성, 도체특성 및 분 내 구리와 아연 함량에 미치는 영향)

  • Kim, Y.H.;Shin, S.O.;Park, J.C.;Jung, H.J.;Cho, J.H.;Chen, Y.J.;Yoo, J.S.;Moon, H.G.;Ji, S.Y.;Kim, I.C.;Lee, S.J.;Kim, I.H.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.79-88
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    • 2008
  • This study was conducted to evaluate effects of dietary Escherichia coli phytase supplementation on growth performance, carcass quality and excretion of copper and zinc concentrations in finishing pigs. The total of seventy two [(Landrace×Yorkshire)×Duroc] pigs(65.43±0.72 kg in average initial body weight) were used in 56 days assay. Dietary treatments included 1) CON (basal diet) 2) P5(basal diet+phytase 0.05%) and 3) P10 (basal diet + phytase 0.1%). There were three dietary treatments with six replicate pens per treatment and four pigs per pen. During the overall periods, ADFI(Average daily feed intake) was increased (P<0.05) in phytase supplementation treatments compared to CON treatment. At the 5th week, dry matter, nitrogen and ash digestibilities were higher in P5 treatment than in CON and P10 treatments(P<0.05) and CON treatment showed the lowest effect on nutrition digestibility(P<0.05). However, phosphorus digestibility was higher in both phytase supplementation treatments than in CON treatment(P<0.05). The pH of M. logissimus dorsi was significantly higher in CON treatment than phytase supplementation treatments(P<0.05). L* value of M. logissimus dorsi muscle color was significantly increased(P<0.05) in P10 treatment compared to CON and P5 treatments. Also, a* value was increased (P<0.05) in CON treatment compared with phytase supplementation treatments. However, excretion of copper and zinc concentrations were no significant difference among the treatments. In conclusion, the effect of Escherichia coli phytase showed in ADFI, digestibilities, pH and color of meat in finishing pigs.

Variation of Cadmium and Zinc Content in Rice and Soil of the Mangyeong River Area (만경강 유역의 토양과 수도체중 Cd 및 Zn 함량의 변화)

  • Kim, Seong-Jo;Baek, Seung-Hwa;Kim, Un-Sung;Yoon, Ki-Woun;Moon, Kwang-Hyun;Kang, Gyeong-Won
    • Korean Journal of Environmental Agriculture
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    • v.13 no.2
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    • pp.142-150
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    • 1994
  • To investigate differences in Cd and Zn contents of paddy soils and rice plants polluted by the municipal and industrial waste water in the Mangyeong River Area, soil and plant samples were collected at several distances from the main inlet and at different depths of the soil. Soil samples were extracted with $4M-HNO_3$ and plant samples were digested with a mixture of $HNO_3$and $HClO_4$for analyzing heavy metals by atomic absorption spectrophotometry. The contents of Cd and Zn in soils ranged from 0.38 to 1.17 and from 33.8 to 464.6mg kg^{-1}, respectively. The average Cd level in 1990 was less than that in 1982, but the Zn level in 1990 was higher than that in 1982 in general. No variation in Cd contents was observed in soils at the different distances from the source of waste water, but Zn contents in soils were lower with the increasing distances from the source of waste water. A significant correlation was observed among Cd content, OM, available silicate, CEC and $Ca^{++}$. Similar results existed among Zn content of 1982, OM and $Ca^{++}$. The Cd content in subsurface soils of 1992 was significantly correlated with Zn, Cu, and Pb in soils, and the Zn content in soils was significantly correlated with the Cu and Pb in soils, regardless of years. The Cd content in leaf blades of rice was more than seven times higher than that in brown rice. The Zn content in rice was higher than that in leaf blades and in panicle axis. The Cd content in panicle axis and the Zn content in all parts of rice were correlated with Zn, Cu and Pb contents in soils. The Cd and Zn contents in brown rice ranged from 0.10 to 0.90mg $kg^{-1}$ and from 4.2 to 95.9mg $kg^{-1}$ in the Mangyeong River Area, respectively.

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The Study on the Confidence Building for Evaluation Methods of a Fracture System and Its Hydraulic Conductivity (단열체계 및 수리전도도의 해석신뢰도 향상을 위한 평가방법 연구)

  • Cho Sung-Il;Kim Chun-Soo;Bae Dae-Seok;Kim Kyung-Su;Song Moo-Young
    • The Journal of Engineering Geology
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    • v.15 no.2 s.42
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    • pp.213-227
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    • 2005
  • This study aims to assess the problems with investigation method and to suggest the complementary solutions by comparing the predicted data from surface investigation with the outcome data from underground cavern. In the study area, one(NE-1) of 6 fracture zones predicted during the surface investigation was only confirmed in underground caverns. Therefore, it is necessary to improve the confidence level for prediction. In this study, the fracture classification criteria was quantitatively suggested on the basis of the BHTV images of NE-1 fracture zone. The major orientation of background fractures in rock mass was changed at the depth of the storage cavern, the length and intensity were decreased. These characteristics result in the deviation of predieted predicted fracture properties and generate the investigation bias depending on the bore hole directions and investigated scales. The evaluation of hydraulic connectivity in the surface investigation stage needs to be analyze by the groundwater pressures and hydrochemical properties from the monitoring bore hole(s) equipped with a double completion or multi-packer system during the test bore hole is pumping or injecting. The hydraulic conductivities in geometric mean measured in the underground caverns are 2-3 times lower than those from the surface and furthermore the horizontal hydraulic conductivity in geometric mean is six times lower than the vertical one. To improve confidence level of the hydraulic conductivity, the orientation of test hole should be considered during the analysis of the hydraulic conductivity and the methodology of hydro-testing and interpretation should be based on the characteristics of rock mass and investigation purposes.

Effects of Split Nitrogen Application on Growth Characters, Yield Potential and Feed Value in Jeju Italian Millet (제주조의 질소분시 횟수에 따른 생육반응, 수량성 및 사료가치 변화)

  • Cho, Nam-Ki;Kang, Young-Kil;Song, Chang-Kil;Ko, Dong-Hwan;Cho, Young-Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.23 no.1
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    • pp.37-42
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    • 2003
  • This study was conducted at a volcanic ash soil in the Experimental Farm of Cheju national university from May 1, 2000 to August 25, 2000 to determine the optimum frequency of split N application for. forage production of Jeju Italian millet(Setaria italica Beauvis). N .rate was applied with 200kg N/ha, and frequencies of the split application were 1. 2, 3, 4 and f times. Days to heading was 87 days in the N applied plot all at once, was delayed to 93 days at the five times split-applied plot. Plant height was the greatest (143cm) at the four times split-applied plot, but above o. below that was short. Leaf length, number of leaves and nodes were a similar tendency to plant height. SPAD(Soil Plant Analysis Development) reading values rose 34.3∼36.2 as N was split-applied from one to five times. Fresh forage, dry matter, crude Protein and TDN yield at the H split-applied to four times increased 33.08∼5l.50MT/ha, 9.94∼13.36MT/ha, 0.93∼1.70MT/ha and 5.06∼7.28MT/ha, respectively, but at the five tines split-applied plot decreased to 49.33MT/ha, 12.69MT/ha, 1.65MT/ha and 6.98 MT/ha, respectively. As the increasing of N split-applied. crude protein, crude fat NFE and TDN content increased 9.4∼13.0%, 1.5∼l.9%, 44.5∼45.5% and 50.9∼55.0%, respectively, whereas crude fiber and crude ash content decreased 35.3∼31.6% and 9.3∼8.3, respectively.

The Evaluation of Trace Minerals Levels of Pasture Soils and Forages in Jeju (제주지역 목장 토양 및 조사료 자원의 미량광물질 함량 평가)

  • Lee, Chong-Eon;Park, Myung-Hee;Park, Nam-Keon;Park, Hyung-Soo;Oh, Woon-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.1
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    • pp.29-36
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    • 2007
  • A study was conducted to determine the trace minerals (Cu, Fe, Cd, Zn, Mo, Se, Mn, Cr, Co, Ni) levels in pasture soils and forages collected in Jeju area. Chemical characteristics and total or soluble trace minerals levels in 187 pasture soil samples (76 very dark brown and 111 black soils) were measured. Total trace minerals contents in hay samples of 60 Italian ryegrass, alfalfa and mixture grasses each were assessed. The pasture soils of Jeju were characterized to have low pH (5.1) and to contain low levels of available $P_2O_5$ (20.5 mg/kg) and exchangeable Ca, Mg, K, Na (2.6, 0.9, 0.5, 0.2 cmol+/kg, respectively) when comparing to upland soils of Jeju or Korean mainland. All trace elements in total or soluble analysis of pasture soils were detected, and there was a big difference between total and soluble levels. The pasture soils tended to have the higher total Fe, Mn, Ni and Zn contents. Cr in all forages was not detected, but other trace minerals levels showed normal range. The hay samples of Italian ryegrass and mixture grasses produced in Jeju tended to contain higher Mn (105 vs 23 mg/kg) and lower Mo (2.7 vs 4.9 mg/kg) than those of alfalfa hay imported from USA. Results show that trace minerals of pasture soils and forages in Jeju seem to be not deficient, indicating that supplementation of some trace minerals are not always necessary in diets for grazing animals and should be done after careful evaluation of diets with regard to concentrations and biological availability of essential elements.

The Development of Korean Traditional Wine Using the Fruits of Opuntia ficus-indica var. saboten - I. Characteristics of Mashes and Sojues - (손바닥 선인장 열매를 이용한 전통주 개발 - I. 전통주 제조기법을 이용한 발효주 및 증류주의 특성 -)

  • Bae, In-Young;Yoon, Eun-Ju;Woo, Jeong-Min;Kim, Joo-Shin;Yang, Cha-Bum;Lee, Hyeon-Gyu
    • Applied Biological Chemistry
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    • v.45 no.1
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    • pp.11-17
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    • 2002
  • Fermentation characteristics with/without nitrogen source and quality of the fruit distillate of Opuntia ficus-indica var. saboten were investigated during the manufacturing process of a Korean traditional liquor. As the fermentation period increased, acidity, brix degree, and alcohol concentration increased, whereas pH and contents of reducing sugar decreased. Acidity, pH, and brix degree were higher, whereas the content of reducing sugar lower, in the nitrogen source-added distillate than in the distillate without nitrogen source. The growth of yeast increased, while that of bacteria decreased; this trend was more prominent with the addition of a nitrogen source. Sojues, distilled from two types of mashes and diluted with $H_2O$ and tails of distillate into 22% alcohol concentration, showed pH $3.7{\sim}4.0$, acidity $0.02{\sim}0.10$, and $5.4{\sim}6.1$ $^{\circ}Brix$. Analysis through GC using direct injection methods revealed common volatile flavor compounds in sojues, including acetaldehyde, acetyl acetone, acetic acid ethyl ester, ethyl alcohol, 2-propyl alcohol, acetone, n-propyl alcohol, butanoic acid methyl ester, 2-phenyl ethanol, thymol, acetic acid phenyl ester, and vanillic aldehyde. As revealed through the sensory evaluation, no significant difference (p>0.05) in overall acceptability was shown among four experimental groups, while color and flavor showed significant differences(p<0.05).