• 제목/요약/키워드: A. keiskeana

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Quantitative analysis of coumarins in Artemisia keiskeana and A. stolonifera using HPLC/PDA

  • Gia Han Tran;Hyejin Cho;Jae Min Chung;Sanghyun Lee
    • Journal of Applied Biological Chemistry
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    • 제66권
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    • pp.244-249
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    • 2023
  • Artemisia keiskeana and A. stolonifera are plants of the genus Artemisia, distributed in various regions, especially China and Korea. They are renowned as medicinal plants with biological and pharmacological activities. Fraxidin, isofraxidin, and daphnoretin are coumarins present in Artemisia spp.; however, research on them is limited. Therefore, this study was carried out to quantify the content of these compounds in the aerial parts of A. keiskeana and A. stolonifera in different regions in Korea. High-performance liquid chromatography was performed with a photodiode array detector and a reverse-phase INNO column. A. stolonifera only contained fraxidin with the highest amount found in Yongmun commune. A. keiskeana cultivation in Soyang commune gave the highest fraxidin and daphnoretin content. However, isofraxidin was not present in all samples. The findings suggest that the concentrations of the three compounds may differ depending on the growth site and provide a foundation for future studies.

잎무늬종 맑은대쑥의 줄기삽목시 삽수부위, 용토 및 오옥신 처리가 발근에 미치는 영향 (Effect of Cutting Position, Media and Auxin on Rooting of Leaf Variegated Artemisia keiskeana Stem Cuttings)

  • 윤희정;이종석
    • 화훼연구
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    • 제18권1호
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    • pp.29-32
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    • 2010
  • 본 연구는 우리나라 자생의 잎무늬종 맑은대쑥(Artemisia keiskeana)을 원예 및 조경용으로 개발하기 위하여 삽목 시 삽수부위, 용토의 종류 및 오옥신류의 처리가 삽수의 발근에 미치는 영향을 구명하고자 하였다. 삽수부위별 처리에 있어서 신초의 정단부보다 중간부와 기부를 삽수로 이용하는 것이 지상부와 지하부의 생육에 좋았으며 특히 중간부의 경우, 발근율이 68.3%로 가장 높았다. 삽목용토에 있어서 피트모스는 발근시작일이 가장 늦었고, 지상부와 지하부의 생육, 발근율과 생존율 모두 낮았던 반면, 버미큘라이트에서는 발근시작일이 가장 빨랐고 지상부와 지하부의 생육도 가장 효과적이었으며, 발근율 88.3%, 생존율 99.2%로 용토처리 중 가장 좋았다. 오옥신 처리를 한 경우, 대조구에 비해 발근율과 생육이 우수했다. 대조구는 발근율이 41%로 낮았으나 처리를 한 경우 발근율이 80%이상으로 현저히 높았다. NAA를 처리한 경우가 IBA를 처리한 것에 비해 근장과 근수를 포함한 지하부의 생육이 좋았고, 농도가 증가할수록 생존율과 발근율이 높았으며 지하부의 생육도 좋았다.

춘천지역의 참취 및 삼지구엽초의 자생지 식생 및 환경특성 (Vegetation and Environment in Natural Habitats of Aster scaber and Epimedium koreanum around Chunchon)

  • 박병재
    • 한국자원식물학회지
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    • 제10권4호
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    • pp.422-427
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    • 1997
  • 유용자원식물의 자생지 재배기술의 확립을 위한 기초자료를 얻고저 참취와 삼지구엽초의 자생지 식생 및 환경(생태 및 토양화학성분) 조사를 실시한 결과는 다음과 같다. 1. 참취자생지인 춘천 A. 연엽산 A 그리고 연엽산 B에서의 종수는 20, 26, 25종이었고 삼지구엽초 자생지인 춘천 B, 북방면 그리고 대룡산에서는 15, 32, 26종이었다. 참취 자생지의 우점종은 춘천 A, 연엽산 A, 연엽산 B에서 맑은대쑥, 애기나리, 실새풀 등이었으며 삼지구엽초의 자생지인 춘천 B, 북방면, 대룡산에서는 고사리, 맑은대쑥, 애기나리 등이 우점하였다. 2. 자생지의 토양산도는 4.5-5.3 범위였으며 유기물 함량은 1.59(춘천A)$\sim$5.5(연엽산 B)이었다. 암모니아태 질소 및 질산태 질소함량은 참취 자생지와 삼지구엽초 자생지 토양에서 평균 각각 0.0043mg/kg, 0.0025mg/kg이었다. 3. 자생지 토양의 $NH_4-N$의 성분은 참취의 자생지의 토양성분이 삼지구엽초의 자생지 토양성분보다 약간 높은 수치를 보였다. 그밖의 성분의 차이는 크게 나타나지 않았다.

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한국산 쑥속의 체세포 염색체수에 의한 분류학적 연구 (A taxonomic study of Korean Artemisia L. using somatic chromosome numbers)

  • 박명순;장진;정규영
    • 식물분류학회지
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    • 제39권4호
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    • pp.247-253
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    • 2009
  • 한국산 쑥속 20분류군의 분류를 위하여 체세포 염색체수를 조사하였다. 본 연구에서 취급된 분류군들의 체세포 염색체수는 2n = 16, 18, 34, 36, 50, 52, 54로서 기본 염색체수는 x=8, 9, 10, 13, 17이었으며, 실제비쑥(A. japonica var. angustissima 2n = 36)의 체세포염색체수는 본 연구에서 처음으로 밝혀졌다. 사철쑥 (A. capillaris 2n = 18), 섬쑥(A. japonica var. hallaisanensis 2n = 36), 갯제비쑥(A. japonica subsp. littoricola 2n = 36), 개똥쑥(A. annua 2n = 18), 개사철쑥(A. carvifolia 2n = 18), 큰비쑥(A. fukudo 2n = 16), 맑은대쑥(A. keiskeana 2n = 18), 넓은잎외잎쑥(A. stolonifera 2n = 36), 그늘쑥(A. sylvatica 2n = 16), 물쑥(A. selengensis 2n = 36), 산쑥 (A. montana 2n = 52), 뺑쑥(A. lancea 2n = 16), 산흰쑥(A. sieversiana 2n = 18) 등의 13분류군의 염색체수는 기존 보고와 일치하였으며, 기존 보고와 다르게 파악된 종류는 제비쑥(A. japonica var. japonica 2n = 18, 36 vs 2n = 36), 더위지기(A. sacrorum 2n = 18, 54 vs 2n = 54), 덤불쑥(A. rubripes 2n = 16, 34 vs 2n = 16), 쑥(A. indica 2n = 34, 36 vs 2n = 34), 참쑥(A. codonocephala 2n = 18, 50, 54 vs 2n = 50), 황해쑥(A. argyi 2n = 34, 36, 50 vs 2n = 34)의 6분류군이었다. 한국산 쑥속의 체세포 염색체수는 제비쑥, 더위지기, 참쑥, 황해쑥, 산쑥, 그늘쑥의 분류에 매우 유용한 형질이었다.

Species Composition and Structure of the Oriental Arbor-vitae (Thuja orientalis L. Forest in Daegu, Southeastern Korea

  • Cho, Hyun-Je;Bae, Kwan-Ho
    • The Korean Journal of Ecology
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    • 제24권4호
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    • pp.205-211
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    • 2001
  • The floristic composition and structure of the Oriental Arbor-viate forest, natural monument no. 1, were investigated in the Daegu city, southeastern Korea. Vegetation stratification have four layers including tree (>5m), subtree (2m-5m), shrub (1m-2m) and herbs (<1m), which occupied 63.8%, 10.7%, 12.1% and 49.4%, respectively. The vegetation of the study area were divided into Artemisia keiskeana - Quercus variabilis and Pyrrosia linearifolia-Thuja orientalis (OAV) communities. Frequency distribution for diameter classes of the Oriental Arbor-vitae population showed a reverse-J shape. The result suggests that the OAV forest of this site might maintains continuously the present state. The annual radial growth of OAV, Pinus densiflora and Quercus variabilis, the dominant species of the present site showed 0.29, 1.01 and 1.28 mm/year, respectively. Competition of OAV with theh other species including P. densiflora and Q. variabilis could influence negatively on the growthand survival of OAV forest in this site.

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관악산의 산림군락에 관한 식물사회학적 연구 (A Phytosociological Study of the Forest Communities on Mt. Kwanak, Seoul)

  • 이병굉
    • Journal of Plant Biology
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    • 제15권1호
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    • pp.1-12
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    • 1972
  • As part of the study of the structure and the successional trends of our temperate forests, a quantitative survey was made of the relatively well conserved forest stands on Mt. Kwanak, Seoul. Three community types were observed; the Pinus densiflora-Quercus mongolica community, the Pinus densiflora community, and the Quercus mongolica community. Evidence suggests that the dominance of Pinus densiflora and quercus mongolica will continue in the Pinus densiflora-Quercus mongolica community, however, in the Pinus densiflora community the dominant species, Pinus densiflora, is now being replaced by Quercus mongolica. The trend toward an increased in Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus cordita, and Acer mono is observed in the Quercus mongolica community. An analysis of the overall composition of the forest reveals that the upper story is mostly occupied by Quercus mongolica and Pinus densiflora, the shrub layer by Rhododendron schlippenbachii and Lespedeza maximowiczii, and the herb layer by Spodiopogon cotulifer and Artemisia keiskeana. Quercus mongolica may cover most part of the forest, gradually excluding Pinus densiflora. Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus coradata, and Acer mono are increasing in importance with the potentiality of developing into major canopy components. With the diversification of the physical habitat Cephalotaxus koreana and Magnolia sieboldii, the species which had almost completely been eliminated form the forest through the severe interferences, have reappeared.

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천마산 동부릉선 남북사면의 삼림식생의 구조적 특성에 관한 비교 연구 (A Comparative Study on the Structural Characteristics of the Forest Vegetation at the Southern and Northern Slopes of the Eastern Ridge in Mt. Cheonma, Central Korea)

  • Lee, Nam-Ju;Yang-Jai Yim
    • The Korean Journal of Ecology
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    • 제11권4호
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    • pp.213-225
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    • 1988
  • The plant communities of the eastern ridge in Mt. Cheonma, Central Korea, Quercus mongolica, Q mongolica-Acer pseudo-sieboldianum, and Q. mongolica-Rhododendron schlippenbachii communities in the northern slope and Q. variabilis and Q. cutissima communities in the southern slope were detected by Zurich-Montpellier method. The undergrowth of Q mongolica community in the northern slope was dominated by Ainsliaea acerifolia, Carex siderosticta, Astilbe chinensis var. davidii, Heloniopsis orientalis, Pseudostellaria palibiniana, ranging 3.3 to 2.2 in dominance-sociability, while the those of Q variabilis and Q acutissima in the southern slope were dominated by Spodiopogon cotulifer, Artemisia keiskeana, Carex humilis, Atractylodes japonica, Lysimachia clethroides and Disporum smilacinum with similar degree in dominance-sociability to that of Q mongolica community. The two communities were considered as regenerating forest after severe disturbance judging by their age structure. The relationship between Shannon's diversity index (H') and Simpson's dominance index (i) was in reciprocal proportion in both two communities. The Q. mongolica and Q. variabilis communities were showed 13 and 12 m respectively. Seasonal changes of undergrowth coverage in the two communities were seemed to be affected especially with soil moisture among environmental factors such as temperature, light intensity, relative humidity and organic matter.

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서울근교 자연생 소나무림에 대한 Ordination 방법의 적용 (An Application of Ordination to semi-Natural Pine (Pinus densiflora) Stands nearby Seoul Area)

  • Cho, Yoon Shin;Kye Chil Oh
    • The Korean Journal of Ecology
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    • 제10권2호
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    • pp.63-80
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    • 1987
  • Detrended correspondence analysis(DCA) and two way indicator species analysis(TWINSPAN) were applied to Pinus densiflora stands nearby Seoul area in order to classify them into several groups based on herbaceous species and environmental relationships. The eighteen Pinus densiflora stands were perferenially selected, In each stand, 30 to 60 quadrats, (20X20)cm, were randomly estabilished. In each quadrat, all stems for herbaceous plants were recorded by species and their covers were measured. In addition environmental factors such as soil depth, thickness of litter layer, soil compressibility, tree density, basal area, elevation, slope and exposure were detemined for each stand. Four to twelve soil cores were collected and physical and KDICical properties were determined. This survey was conducted form 15, July, 1985 to 22, September, 1985. Analysis of the vegetation and environmental data were performed with DECORANA and TWINSPAN. Stand ordination (DECORANA) and classification (TWINSPAN) based on herbaceous species suggest that the stands can be classified into five groups in terms of herbaceous species composition and environmental relationships. Type I, Type II, Type III, Type IV and Type V were classified to SUBMESIC-CLO-SED-CANOPY (Artemisia keiskeana, Carex nanella), MESIC-CLOSED CANOPY (Spodiopogon sibiricus, Miscanthus sinensis, Carex nanella, Pteridum aquilinum, Melampyrum rosium), SUBMESIC-OPEN CANOPY (Carex nanella, Spodiopogon sibiricus, Miscanthus sinensis), XERIC-CLOSED CANOPY (Carex nanella, Spodiopogon sibiricus, Carex nanella), and XERIC-OPEN CANOPY (Arundinella hirts, Spodiopogon sibiricus, Carex nanella), respectively. These results were consitent with environmental trends on stan ordination. Application of the ordination and classifiaction to Pinus densiflora stands were effective as a approach on obtaining perliminary ecological information.

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Induction of Quinone Reductase, an Anticarcinogenic Marker Enzyme, by Medicinal Herb Extracts

  • Kwon, Chong-Suk;Kim, Ji-Hyeon;Son, Kun-Ho;Kim, Young-Kyoon;Lee, Jeong-Soon;Lim, Jin-Kyu;Kim, Jong-Sang
    • Preventive Nutrition and Food Science
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    • 제7권4호
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    • pp.358-366
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    • 2002
  • To search for novel cancer preventive agents, we assessed the quinone reductase (QR)-inducing activities of medicinal herb extracts in cultured murine hepatoma cells (hepalclc7 cells). Among 216 herb extracts tested in this study, 8 kinds of herbal extracts were found to induce QR activity in hepalclc7 cells by more than 2-fold when used at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The methanol extracts of Aster koraiensis NK and Pulsatilla koreana Nakai induced QR by 252 and 223 % , respectively, at the concentration of 25 $\mu\textrm{g}$/$m\ell$. Most of the herbal extracts with QR inducing-activity increased the enzyme activity in a typical dose-dependent manner. The QR activity in BP$^{r}$ cl cells was induced move than 50 % by the extracts of Pulsatilla koreana Nakai, Inula helenium, Physalis alkekengi var, francheti (Masters) Makino, Chrysanthemum zawadskii var. latilobum Kitamuva, Auemisia keiskeana Miquel, Chfsanthemum boreale Makino. In conclusion, hlsatilla koreana Nakai, Aster koraiensis N.K, and Chfsanthemum zawadskii var. iatilobum Kitamura, which showed relatively high QR induction, merit further animal study to evaluate their potential as cancer preventive agents.

소나무 모수림 시업지의 하층식생 종 조성과 소나무 천연갱신양상 (Understory Species Composition and Pinus densiflora Natural Regeneration in Pinus densiflora Stands Regenerated by Seed-Tree Method)

  • 변성엽;윤충원
    • 한국산림과학회지
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    • 제107권1호
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    • pp.25-34
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    • 2018
  • 본 연구는 소나무 모수림 시업지를 대상으로 시업에 의한 하층식생 비교 및 갱신 치수의 생장초기과정을 구명하고자 수행하였다. 조사구는 시업지 12개소(요형지 6개소, 사면부 6개소)와 대조구 6개소를 각각 설치하였으며, 각 조사구에 대해 식생조사 및 각 치수의 절간 높이를 측정하였다. 그 결과, 모수림 시업지의 하층에는 싸리, 산초나무, 광대싸리, 조록싸리, 국수나무 등의 관목성 식물과 억새, 가는잎그늘사초, 큰기름새, 맑은대쑥 등의 초본성 식물, 칡, 청가시덩굴, 마, 다래, 왕머루, 산딸기 등 덩굴성 식물이 상당수 이입하고 있었다. 특히, 덩굴성 식물은 요형지에서 대조구와 사면부에 비해 상대적으로 많은 양이 이입되고 있었다. 매목조사 결과, 소나무 갱신치수는 비피압목 3,175본/ha, 피압목 7,842본/ha으로 나타났으며, 요형지의 경우, 사면부에 비해 갱신치수의 발생 밀도가 매우 낮았는데, 이는 덩굴성 식물에 의한 영향으로 보여 이들 덩굴식물에 대한 구체적인 제거방안이 필요할 것으로 판단되었다. 또한 비피압목과 피압목의 절간생장은 꾸준히 증가하는 것으로 나타났으며, 특히 비피압목의 절간생장속도는 피압목에 비해 빠른 것으로 나타났다. 이는 갱신치수가 주변 식물과의 경쟁에서 이겨낼 능력을 갖추게 되면 급격한 생장을 이루는 것으로 판단되므로, 모수림 시업 초기에는 풀베기작업 등을 통한 집약적인 관리가 필요할 것으로 판단되었다.