• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.845-855
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• 2015

The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60℃ and kept 45% enzyme activity after 1 h incubation at 70℃. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).

• Journal of Applied Biological Chemistry
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• 제57권2호
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• pp.137-140
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• 2014

A gene encoding thermostable pectinase (TmPec) was isolated from hyperthermophilic microorganism, Thermotoga maritima. The open reading frame (ORF) of TmPec gene is 1,104 bp long and encodes 367 amino acid residues with a molecular weight of 40,605 Da. To analyze the enzymatic activity and biochemical properties, the ORF of TmPec gene excluding putative signal sequence of 27 amino acids was introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. Protein concentration of purified recombinant TmPec was 1.1 mg/mL with specific activity of 56 U/mg protein on pectin. The recombinant TmPec showed the highest activity at around $85-95^{\circ}C$, and at around pH 6.5. It was stable at temperature below $85^{\circ}C$. In the presence of $Ca^{2+}$, the activity of recombinant TmPec was increased to 146.3% of normal level. In contrast, $Ba^{2+}$ and Mn2+ showed strong inhibition to the recombinant TmPec.

• 대한디지털의료영상학회논문지
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• 제6권1호
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• pp.31-40
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• 2003

Purpose : In a PACS(Picture Archiving Communications System) environment, which is a very important component in a digital medical environment, the compression of digital medical images is a necessary and effective feature. In a current system where JPEG is applied to the compression of medical images, this study is to examine effectiveness and suitability when the JPEG2000, a more advanced compression algorithm for still images, is applied to the compression of medical images. In this thesis, we attempt to address the compressibility for effective clinical usage when compressing medical images, applying the objectivization of clinical evaluation as a function of compressibility. In the experiment al method, the compression was applied at a fixed rate using JPEG2000, and the n the result was compared with compressed images by JPEG. Method : For the performance evaluation, we choose SNR(Signal to Noise Ratio) measurement of an objective evaluation of definition and analyze a subjective evaluation by the ROC(Receiver Operating Characteristic) method. The results of the experiment showed that in the case of JPEG2000 there is hardly any distortion of images, even at high compression ratio(100:1), while regarding noise, the SNR remains around about 40dB, which is also relatively high. Before reading by reference to evaluative materials concerning objective compressed images, it is impossible to apply high compression to images : however, after reading, this can be applied to images that have already existed for some time.

• BMB Reports
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• 제37권4호
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• pp.429-438
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• 2004

Complementary DNAs encoding $\alpha$-amylases (Amyl I, Amyl III) and glucoamylase (GA I) were cloned from Aspergillus awamori KT-11 and their nucleotide sequences were determined. The sequence of Amyl III that was a raw starch digesting $\alpha$-amylase was found to consist of a 1,902 bp open reading frame encoding 634 amino acids. The signal peptide of the enzyme was composed of 21 amino acids. On the other hand, the sequence of Amyl I, which cannot act on raw starch, consisted of a 1,500 bp ORF encoding 499 amino acids. The signal peptide of the enzyme was composed of 21 amino acids. The sequence of GA I consisted of a 1,920 bp ORF that encoded 639 amino acids. The signal peptide was composed of 24 amino acids. The amino acid sequence of Amyl III from the N-terminus to the amino acid number 499 showed 63.3% homology with Amyl I. However, the amino acid sequence from the amino acid number 501 to C-terminus, including the raw-starch-affinity site and the TS region rich in threonine and serine, showed 66.9% homology with GA I.

• 식물조직배양학회지
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• 제24권2호
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• pp.93-97
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• 1997

Linoleic acid를 linolenic acid로 전환시키는 지방산불포화효소의 유전자(fad3)를 유채의 fad3 DNA probe를 이용한 plaque hybridization방법으로 $\lambda$ZAPII Arabidopsis thaliana cDNA expression library로부터 분리하였으며 1.8 kb-EcoRI DNA조각을 지니는 lambda clone을 pGEM7으로 subcloning하여 염기서열을 분석하였다. 그 결과로부터의 아미노산서열분석에 의하면 fad3 유전자는 open reading frame이 386개의 아미노산으로 이루어졌으며 44,075 Da의 분자량이 예측되고 있다. 엽록체의 $\omega$-3 지방산불포화효소(fad7)와 endoplasmic reticulum의 지방산불포화효소(fad2)와의 비교시 각각 70%와 58%의 유사성이 나타났다. 특히 82-151의 아미노산서열과 276-333의 아미노산지역은 보존성이 높았으며 이는 불포화지방산효소의 기능에 필수적인 지역으로 보여진다. 한편 대장균을 이용한 IPTG에 의한 Fad3단백질의 유도생성은 대장균의 생육에 독성효과를 나타내는 것으로 나타났다.

• 전기학회논문지
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• 제56권7호
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• pp.1261-1267
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• 2007

Although LEDs have been used in various applications with improving the brightness and luminous efficacy, the electrical and optical characteristics of high power LED varies at different temperature and starting time. In this paper, optimal heat sink and apparatus design were conducted using IR camera and ICEPAK on the LED bulb consisting with fourteen LED array. The temperatures of heat sink and LED device of the designed LED bulb without cooling pan were $74^{\circ}C\;and\;96.8^{\circ}C$, respectively, showing in good themal characteristics. For high efficiency driving circuit of LED array adopted optimal heat sink design, driving circuits of constant voltage and current circuits were suggested and fabricated. As a result the efficacy of all driving circuits showed more than 20 lm/W. Also, the constant current circuits are suitable for signal lamp, hallway lamp, and flash lamp having short operating time(about 30 min). On the other hand, a reading light and indoor lamp having long operating time can be controlled by constant voltage circuit.

• 전기학회논문지P
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• 제61권1호
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• pp.55-58
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• 2012

By using EEG and HRV, subjects were estimated on their psychological and physiological reaction when reading psychrometric chart in 7 point century font, in an environmental test room in the condition of temperature 25[$^{\circ}C$], relative humidity 50[RH%], air current speed 0.02[m/sec], giving variation in illuminance to 0.1, 300, 600, 1000, 1300 and 1600[lux]. As a result, it was at 1300[lux] that absolute ${\alpha}$ wave, SMR, SDNN were most vitalized, and also both sides ${\alpha}$ wave asymmetry index, SEF50, HRT, stress and fatigue degree were at the lowest. It was found that a certain illuminance which minimizes psychological stress and fatigue degree while enhancing concentration and task achievement stably does exist.

• 대한산업공학회지
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• 제30권1호
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• pp.58-64
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• 2004

In order to determine the level of safety on highway driving, the relationship between the psychophysiological signal and driving condition was investigated. In particular, a Demand-Effort model was conceptualized and used in this study to diagnose the suitability of driving by reading the patterns of psychophysiological signals. To run the model, threshold values were determined to categorize the high, moderate and low level of effort. To examine the sensitivity of the model, a cross-validation process was performed by collecting additional data. Further investigation need to be conducted to improve the sensitivity of the model for practical application.

• Parasites, Hosts and Diseases
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• 제31권3호
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• pp.285-292
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• 1993

간흡충 total RNk에는 많은 량의 185 rRNA가 함유되어 있었지만 285 rRNA는 그 양이 매우 적었다. 약 $8{\;}{\mu\textrm{g}}의{\;}poly{\;}(A)^{+}$ mRNAS부터 합성된 double-stranded CDNA는 대부분이 0.4-4.2 kb 크기이었으며 9.5 kb에 달하는 것도 있었다. 이미 보고되어 있는 tropomyosin의 amino산 서열을 기준하여 5개의 degenerated oligonucleotide (sense primer 2개와 antisense primer 3개)를 합성하였다. TotalcDNA를 template로 하고 sense primer와 antisense primer를 조합하여 실시한 PCR 산물 중에서 580 bp 크기의 특이 유전자가 나타났다. 만손주혈흡충의 tropomyosin CDNA를 탐색자로 써서 Southern hybridization했을 때 이 유전자만이 검출되어서. 이 유전자는 간횹충 tropomyosin (CSTM) CDNA의 일부분일 가능성이 높다고 생각되어 sequencing vector인 POEM-3Zf(-)에 cloning한 다음 염기서열을 결정하였다. nRf 증폭된 CSTM CDNA는 크기가 575 bp이었으며 191개의 predicted amino산 서열은 한 개의 open reading frame을 갖고 있었다 CSTM CDNA의 amino산 서열은 만손주혈흡충 tropomyosln과 86.3%. Trichosoonvk: colhnfornis tropomyosin과 51.1% 의 유사성을 갖고 있었다. 이 CSTM cDNA fragment는 앞으로 간흡충 cDNA library를 screening하여 완전한 CnM CDNA를 cloning하기에 좋은 probe로 쓰일 것으로 예상된다.

• 한국산학기술학회논문지
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• 제12권9호
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• pp.4133-4137
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• 2011

신뢰성 있는 다중태그 인식은 최근 다중태그 애플리케이션 이슈 중의 하나이다. 하지만, 데이터 확보 단계에서 다중태그 리더를 통한 신뢰성 있는 다중태그 인식은 리더간의 충돌, 소음, 태그가 부착된 물건들의 이동 등으로 발생하는 거짓양성인식, 거짓음성인식, 비 인식같은 신뢰성 없는 인식으로 인하여 신뢰서 있는 데이터를 확보하는데 어려움을 겪고 있다. 따라서 본 논문은 다중태그 리더를 통한 인식에서 발생되는 이러한 문제점들을 해결하기 위하여 먼저 성능평가 기준을 소개하고, 1) 수신된 신호 강도 표시기 (RSSI)을 이용한 최소 중첩인식공간 설정방식, 2)시-공간 분할 처리방식, 3) 큰 사이즈의 이중 태그 부착 방식등과 같은 3가지 해결방안을 제시하였다. 그리고 본 논문은 멀티 RFID 리더가 설치된 스마트 사무실에서 태그의 성공 인식률 계산을 통하여 제안된 방법의 성능개선을 보여주었다.