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Effects of Dietary Supplementation of Lactobacillus on Performance, Nutrient Digestibility, Intestinal Microflora, and Fecal $NH_{3}$ Emission in Laying Hens (산란계의 생산성, 영양소 소화율 분의 암모니아 발생량 및 장내 미생물 변화에 대한 유산균의 급여 효과)

  • 김상호;유동조;박수영;이상진;최철환;나재천;류경선
    • Korean Journal of Poultry Science
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    • v.29 no.3
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    • pp.213-223
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    • 2002
  • This study was conducted to investigate the influence of feeding various Lactobacillus on production performance, nutrients digestibility, intestinal microflora, and fecal $NH_{3}$ gas emission in laying hens. Three hundred and sixty ISA Brown layers, 21 weeks of age, were randomly allotted to nine treatments, with low replicates per treatment. Nine treatments consisted of Control(no Lactobacillus), Lactobacillus crispatus avibrol(LCB: KFCC-11195), Lactobaciilus reuteri avibro2(LRB: KFCC-11196), Lactobacillus crispatus avihenl(LCH: KFCC-11197), Lactobacillus vaginalis avihen2(LVH: KFCC-11198). Each Lactobacillus was added at two levels ($10^{4}$and $10^{7}$ cfu/g diets). Egg production, and egg weight were measured daily. A metabolism trial was conducted following the 12-week feeding trial, during which egg qualities, intestinal microflora and fecal $NH_{3}$ gas emission were examined. Egg production and daily egg mass improved significantly by the addition of various Lactobacillus(P<0.05), of which effect was more notable during the latter part of the feeding trial. But, no significant differences were found among Lactobacillus strains and between two levels of supplementation. Egg weight and feed intake showed no difference among all treatments. Feed conversion ratio of birds fed lactobacillus was significantly improved compared to that of the Control(P<0.05), but not different among lactobacillus treatments. Digestibility of crude protein, ether extract and crude ash improved significantly in lactobacillus treatments(P<0.05). However, there were not statistically different by adding levels and strains. Total counts of Lactobacillus spp. in ileum of layers fed Lactobacillus were significantly higher than that of the control, but no consistent trend was found in cecum. There were no significant differences in intestinal yeast and anaerobes counts among all treatments. The Lactobaciilus supplementation did not exert my effect on the eggshell quality and Haugh unit. Fecal $NH_{3}$ gas emission decreased significantly in Lactobacillus treatments, and showed no difference between the two supplementation levels. From the result of this study, it could be concluded that dietary supplementation of Lactobacillus, regardless of their species, Improves the laying performance and decreases the fecal ammonia gas emission. The proper level of supplementation appears to be $10^{4}$ cfu/g of diet.

Effects of Genetic, Physiological, and Other Variations on Yolk Cholesterol Level (난황 콜레스테롤 수준에 미치는 유전적, 생리적 및 기타 변이 요인들의 효과)

  • Y. O. Suk;K. W. Washburn
    • Korean Journal of Poultry Science
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    • v.24 no.4
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    • pp.169-177
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    • 1997
  • Four experiments were conducted to investigate the association of yolk cholesterol level with egg trails, serum cholesterol level, body weight (BW) and breed differences and other variations. Athens Canadian Randombred (ACRB), a commercial layer breed (CL) and two commercial broiler breeds (BR1 and BR2) were used for this studies. Egg weight of CL was heavier by 8.7g per egg than that of ACRB, but the yolk percentage and yolk cholesterol were lower by 7.8% per egg and 5.5mg per g yolk than those of ACRB, respectively. Yolk cholesterol content had no significant relation with the average egg weight or yolk weight in ACRB, but was significantly related with yolk fat level. However, yolk cholesterol level in CL showed inverse relationship with other egg traits compared to that of ACRB. In the CL female group, the association of yolk cholesterol level with BW was significant(P$\leq$0.05) at 25 weeks of age, but was not significant different at 26 wks of age. The relationship of yolk cholesterol with serum was not significantly different at 25 weeks of age, whereas it showed significant negative(-) correlation coefficient(P$\leq$0.01) at 26 wks of age. The association of yolk cholesterol level with egg age in CL population was not high comared to that of other treatments. The serum cholesterol content of CL was not significantly different with BR1 and BR2 populations at 25 weeks of age, but was significantly(P$\leq$0.05) higher than that of BR1 or BR2 at 26 weeks old.

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Effects of Single or Mixed Feeding of Lactobacillus and Yeast on Performance, Nutrient Digestibility, Intestinal Microflora, and Fecal NH$_{3}$ Gas Emission in Laying Hens (유산균과 효모의 단독 및 혼합 급여가 산란계의 생산성, 영양소 소화율, 장내미생물 변화 및 분의 암모니아 발생량에 미치는 영향)

  • 김상호;유동조;박수영;이상진;치철환;성창근;류경선
    • Korean Journal of Poultry Science
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    • v.29 no.3
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    • pp.225-231
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    • 2002
  • Total 240 of ISA Brown layers were employed in this experiment to study the effects of single or mixed feeding of Lactobacillus and yeast on the performance and intestinal microflora of laying hens. They were randomly allocated to six dietary treatments; None(Control), Pichia farinosa(PF), Lactobacillus crispatus avihen1 (LCH), Lactobacillus vaginalis avihen1(LVH), LCH+PF, and LVH+PF. Viable microflora were added to meet 3${\times}$10$\^$6/ cfu PF and 10$\^$7/ cfu Lacrobacillus per g of feed. There were four replicates per treatment, and 10 birds per replicates. Laying performance was recorded for 10 weeks, followed by a metabolism trial during which nutrient utilization, pattern of intestinal microflora and fecal NH$\sub$3/ emission were examined. Egg production and daily egg mass of birds fed either single or mixed microorganisms were significantly higher than those of the control(P<0.05). Egg weight and feed intake were not statistically different among all treatments. However, feed conversion ratio tended to improve by the supplementation of microbes. Digestibility of crude protein, ether extract and crude ash tended to improve in Lactobacillus treatments, however, there were not statistically different. With regards to the number of intestinal microbes, number of anaerobes were increased in microbes feeding group. Eggshell quality of PF layers was significantly poorer than those of the other treatments. No consistent trend was found in Haugh Unit among all treatments. Fecal NH$\sub$3/ gas emission was significantly lower in LVH, LVH+PF and LCH+PF than the other treatments(P<0.05). From the result of this experiment, it could be concluded that single or mixed feeding of Lactobacillus and yeast improves the laying performance and decreases the fecal ammonia gas emission. No synergic effect was found when both microbes were mixed and fed to the layers.

Changes of Albumen Index and Haugh Unit by the Different Position of Thick Albumen Height of Eggs Measured (계란 농후난백의 높이 측정부위에 따른 Albumen Index와 Haugh Unit의 변화)

  • 하정기;김태호;나재천;강보석;하광수;김동혁;이상진
    • Korean Journal of Poultry Science
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    • v.29 no.3
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    • pp.157-170
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    • 2002
  • This experiment was conducted to investigate the change of egg qualify by the height of thick albumen of egg, storage temperature and storage period. The albumen index and Haugh unit were measured and compared among five different storage periods(0, 2, 4, 6, and 10 days), four different storage temperatures($15^{\circ}C$ , $20^{\circ}C$ , $25^{\circ}C$ and $30^{\circ}C$ ), and five different Parts(0, 3, 6, 9, and 12 mm) aparted from egg yolk. The albumen index and Haugh unit were as follows: the albumen index of eggs was significantly(P<0.01) different by the storage temperature and by the measuring position of thick albumen of eggs. It means that the albumen index can be differed by the measuring position of thick albumen even if eggs have a same qualify. For measuring egg qualify in this country, it should be announced that which Parts in thick albumen of eggs are measured. The albumen index of fresh eggs was 0.16. However, the index when measured at 3 hour after oviposition was lower than 0.16. Therefore, it indicates that the criterion of 0.06 lower than 0.16 is required to set for considering practical point during the marketing periods of eggs. When the criterion of albumen index puts 0.06, it is as 100%. Therefore, eggs can be kept the same quality for 6, 4 and 2 days when eggs were stored at $15^{\circ}C$ , $20^{\circ}C$ and 25 ~$30^{\circ}C$ , respectively. When the criterion of Haugh unit score puts 79 as 100%, the eggs can be maintained the quality for 6, 4, and 2 days when eggs are stored at $15^{\circ}C$ , $20^{\circ}C$ and 25~$30^{\circ}C$ , respectively. When the criterion of Haugh unit 55 as 100%, the eggs can be maintained the same quality for 10, 8 and 2 days when eggs are stored at $15^{\circ}C$ , $20^{\circ}C$ and 25~$30^{\circ}C$ , respectively. From the experimental results, it can be considered that calculathing egg quality by the height of thick albumin of eggs which was measured at the attachment Parts of thick albumin layer and yolk of eggs was most reasonable and suitable when considering the marketing periods of eggs.

The Study on Growth Performance and Uniformity of White Korean Native Ducks (백색 토종오리의 성장능력 검정과 균일도 연구)

  • Heo, Kang-Nyeong;Choo, Hyo-Jun;Kang, Bo-Seok;Kim, Sang-Ho;Kim, Chong-Dae;Cha, Jae-Beom;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • v.43 no.1
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    • pp.39-45
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    • 2016
  • This study was carried out to evaluate performance and uniformity of White Korean native ducks collected from two areas in Korea. White ducklings were selected from all ducklings that were hatched from hatchery eggs of Korean native ducks. A total of two hundred forty white ducklings (1-d-old) were used in this work and were divided into 4 groups (4 replicates/group, 15 birds/replicate) with $2{\times}2$ factors (2 groups, J, H; genders, male and female). Weekly body weight of H groups was higher than that of J groups (P<0.05). Also, weekly body weight of male ducks was higher compared to that of female at 6~8 wk old (P<0.05). Uniformity of J groups was higher than that of H groups at the age of 1 wks old (P<0.05), and that of female duck was higher than that of male at the age of 6 wks old (P<0.05). Body weight gain of H group was higher than that of J group (P<0.05), and that of male duck was higher compared to that of female at 6~7 wks old (P<0.05). Feed intake of H group was higher compared to that of J group (P<0.05), but there was no significant difference between male and female on feed intake. On feed conversion ratio, J group was higher than H group at 4~5 wks, 7~8 wks and 0~8 wks old (P<0.05), and feed conversion ratio of male duck was higher than that of female at 1~2 wks, 5~6 wks and 6~7 wks old. Finally, these results was tended to show that performance of H groups was higher than that of J groups. Further researches need to carry out to commercial White Korean native ducks on performance and uniformity as well as meat quality and genetic characteristic.

Effect of Dietary Supplementation of Quercetin on Oxidative Stability of Chicken Thigh (Quercetin의 급여가 육계 다리육의 산화 안정성에 미치는 영향)

  • Jang, Ae-Ra;Park, Jung-Eun;Kim, Sang-Ho;Chae, Hyun-Seok;Ham, Jun-Sang;Oh, Mi-Hwa;Kim, Hyoun-Wook;Seol, Kuk-Hwan;Cho, Soo-Hyun;Kim, Dong-Hun
    • Korean Journal of Poultry Science
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    • v.37 no.4
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    • pp.405-413
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    • 2010
  • This study was carried out to determine the effect of dietary supplementation of quercetin and methoxylated quercetin extracted from onions on oxidation of chicken thigh during cold storage. For 35 days, 1-day-old 320 broiler chicks (Ross) were divided into 8 groups and supplemented the diet; basal diet only (T1), T1 with antibiotics (T2), vitamin E 20 IU (T3), vitamin E 200 IU (T4), quercetin 20 ppm (T5), quercetin 200 ppm (T6), methoxylated quercetin 20 ppm (T7), methoxylated quercetin 200 ppm (T8). After slaughtering the broilers, thigh meats were collected and analyzed the oxidation stability during storage at $4^{\circ}C$ for 7 days. Volatile basic nitrogen (VBN) was ranged from 10.98~15.37 mg% until day 3 and quercetin 20 ppm (T5) significantly reduced (p<0.05) the VBN value of thigh as much as antibiotics treatment group (T2). The supplementation of quercetin at a 200 ppm level significantly reduced 2-thiobarbituric acid reactive substances value when compared with the control. Supplementation of 20 ppm quercetin also affected fatty acid composition of chicken thigh and resulted in increase of C18:1w9 and decrease of C18:2w6 on day 3. In microbes of chicken thigh during storage, supplementation of 20 ppm quercetin significantly reduced total microbes compare to control on day 7 (p<0.05). There was no effect on the number of coliforms in thigh with quercetin diet. From these results, the dietary supplementation of 20 ppm of quercetin may have effects on enhancing oxidation stability.

Effect of Dietary Supplementation of Fermented By-products of Garlic and Onion on Production Performance, Blood Components and Cecal Microflora in Broiler Chicks (발효 마늘 및 양파 부산물의 급여가 육계 생산성, 혈액성상 및 장내 미생물에 미치는 영향)

  • Kang, Hwan-Ku;Seo, Ok-Suk;Choi, Hee-Chul;Chae, Hyun-Suk;Na, Jae-Cheon;Bang, Han-Tae;Kim, Dong-Wook;Park, Sung-Bok;Kim, Min-Ji
    • Korean Journal of Poultry Science
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    • v.37 no.4
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    • pp.433-438
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    • 2010
  • This study investigated the effects of dietary supplementation of the fermented by products garlic and onion on growth performance, blood composition and cecal microflora in broiler chickens. A total four hundred eighty, day old broiler chickens (Ross) were randomly divided into four groups with four replicates of thirty birds each. The treatment groups were negative group (NC, antibiotic-free diet), positive group (PC, basal diet with 0.05% and 0.03% anticoccidials), fermented of onion by product 1.0% group (T1) and fermented of onion by product 1.0% group (T2). The body weight of broilers fed the diets containing fermented by products garlic was higher than the other treatments during overall period. No significant difference were observed on serum chemical composition and blood corpuscle. In the cecal microflira of broiler, the population of the Lactic acid bacteria was showed the higher in chicken fed diets supplemented with fermented of garlic group than other groups (P<0.05). These results suggest the possibility that fermented of garlic and onion by product could be used as the alternative of antibiotics growth promotor of broiler chickens.

Effects of Dietary Supplementation of Domestic Skullcap (Scutellaria baicalensis) Extracts on Performance, Immune Response and Intestinal Microflora in Broiler Chicken (국내 자생 황금 추출물의 첨가 급여가 육계의 생산성, 면역 기능 및 장내 균총 변화에 미치는 영향)

  • Kwon, H.S.;Kim, J.Y.;Kim, J.S.;Lee, B.K.;Lee, S.Y.;Lee, W.S.;Ahn, B.K.;Kim, E.J.;Kang, C.W.
    • Korean Journal of Poultry Science
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    • v.35 no.4
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    • pp.351-359
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    • 2009
  • This study was carried out to investigate dietary effects of extracts of Skullcap (Scutellaria baicalensis) (SCE) grown in Korea on growth performance, immune and physiological responses in broiler chickens. Total of seven-hundred fifty 1-d-old Ross male broiler chicks were divided into five groups and fed control diets (antibiotics medicated or non-medicated commercial diets) or each experimental diet (non-medicated diets containing 0.1, 0.3 or 0.5% SCE) for 5 weeks. The body weight gain and feed conversion rate in the groups fed diets containing 0.1% or 0.3% SCE were significantly improved as compared with those of non-medicated control group (P<0.05). The levels of total cholesterol and HDL-cholesterol of blood were not influenced by feeding the SCE. The average antibody titers against NDV and IBV in the groups fed diets containing SCE were significantly increased compare to those of the control groups (P<0.05). The number of coli form bacteria was significantly reduced by feeding 0.3% or 0.5% SCE as compared to that of non-medication control (P<0.05). The results demonstrated that the SCE used in this study modulated humoral immunity and the profiles of cecal microflora and thus can be used as a potential alternative substance to replace antibiotics for feeding broiler chicks.

Effects of Dietary Energy, Protein on Growth and Blood Composition in Cross-bred with Korean Native Chicks (사료 단백질 및 에너지 수준이 재래닭의 성장과 혈액의 성상에 미치는 영향)

  • Lee, H.S.;Kang, B.S.;Na, J.C.;Ryu, K.S.
    • Korean Journal of Poultry Science
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    • v.35 no.4
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    • pp.399-405
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    • 2009
  • Two experiments were conducted to establish the basic data of feeding system in cross-bred Korean native chicks. A total of seven hundred twenty chicks were replaced the 36 floor pen for the first five weeks with $3{\times}3$ factorial design in Experiment 1. Four hundred eighty of five weeks old chicks were raised from six to ten weeks of age with $3{\times}2$ factorial design in Experiment 2. Dietary ME and CP were formulated to contain 3,000, 3,050, 3,100 kcal/kg and 21, 22, 23%, respectively in Experiment 1 and 3,050, 3,100, 3,150 kcal/kg and 18, 19% in Experiment 2. Weight gain, feed intake were measured and calculated the feed conversion. Blood were collected and analyzed at the end of experiments. In Experiment 1, weight gain showed significantly higher in 3,050, 3,100 kcal/kg treatments than 3,000 kcal/kg treatment (P<0.05), but was not different in CP treatments. Feed intake was statistically high in 3,000 kcal/kg treatment compared with 3,050 and 3,100 kcal/kg ones (P<0.05), and more increased in 21% CP treatments compared to that of 22 and 23 CP treatment (P<0.05). Feed conversion of birds fed 3,050 and 3,100 kcal/kg diet showed much lower than 3,000 kcal/kg treatments (P<0.05). FCR was signicantly improved (P<0.05) in chicks fed diets containing 21 and 22% CP as compared to that fed 20% CP. Blood protein, glucose, and total cholesterol tended to increase in high energy and diet treatments. Blood HDL was increased as dietary energy increased, whereas LDL increased in low CP treatments. In Experiment 2, weight gain was not consistent between treatments, but more increased in 18% CP treatments compared to that of 19% CP treatment from six to ten weeks old in cross bred chicks (P<0.05). Feed intake was similar to the result of weight gain, but more increased in 19% CP treatment than 18% CP treatment (P<0.05). There were no statistically difference in FCR, but seemed to improve as dietary ME increased. Blood total protein and glucose increased as dietary CP was high, but triglyceride and HDL increased in high versus low ME (P<0.05). The results of these experiments suggested that optimum dietary ME and CP, were 3,050, 3,150kcal/kg and 22, 19% for the first five weeks and second one, respectively.

A Comparative Study on Performances and Carcass Traits in Three Major Meat-Type Duck Strains in Korea (국내 사육되는 육용오리 세 가지 계통의 생산성 및 도체 특성 비교 연구)

  • Bang, Han-Tae;Na, Jae-Cheon;Choi, Hee-Chul;Chae, Hyun-Seok;Kang, Hwan-Ku;Kim, Dong-Wook;Kim, Min-Ji;Suh, Ok-Suk;Park, Sung-Bok;Choi, Yang-Ho
    • Korean Journal of Poultry Science
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    • v.37 no.4
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    • pp.389-398
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    • 2010
  • The present experiment was carried out to compare performance, carcass characteristics and meat quality among three major meat-type duck strains in Korea. Two commercial duck (CD) strains, produced from parent stocks (PS) (Cherry Valley and Grimaud), and $F_1$ strains, self-produced by farmers, were used in an 8-week feeding experiment. Both feed conversion ratio and production index were significantly higher in Cherry Valley strain compared with the other two at the ends of 6 and 8 weeks (p<0.05). However, no differences were found in carcass characteristics and meat quality, with the exception of abdominal fat weight where Grimaud were higher than the others. Overall, the results of the current study show that Cherry Valley CD strain had higher performance among three strains tested.