• Parasites, Hosts and Diseases
• /
• v.36 no.4
• /
• pp.255-260
• /
• 1998

To evaluate the biological and biochemical characteristics of Trichomonas vaginalis KT9 isolate, the growth and size of trichomonads, pathogenicity in mouse, protein profiles and proteinase activity were examined after shifting the medium from TPS-1 into TYM. Generation time of trichomonads in TYM medium was 4.5 hr in comparison to TPS-1 with 7.1 hr. Size of trichomonads cultured in TPS-1 medium ($8.5{\;}{\pm}{\;}0.9{\;}{\times}{\;}6.0{\;}{\pm}{\;}0.9{\;}{\mu\textrm{m}}$) was significantly smaller than those in TYM medium ($10.9{\;}{\pm}{\;}1.4{\;}{\times}{\;}8.2{\;}{\pm}{\;}0.9{\;}{\mu\textrm{m}}$). Trichomonads cultured in TYM medium produced subcutaneous abscess in 9 out of 10 mice, whereas those in TPS-1 medium produced abscesses in 2 out of 10 mice. In SDS-PAGE, trichomonad Iysates from both media showed ten common bands. However, trichomonads in TYM medium showed additional bands of 136 kDa, 116 kDa and 40 kDa in comparison to those in TPS-1 with 100 kDa. By immunoblot with T. vaginalis-immunized rabbit sera, T. vaginalis cultivated in both TYM and TPS-1 media showed 5 common bands. and unique bands of 116 kDa. 105 kDa. and 86 kDa were observed in trichomonads in TYM while a 140 kDa band in those in TPS-1. In gelatin SDS-PAGE, trichomonads in TYM degraded gelatin stronger than those in TPS-1. Also protease activity of trichomonads in TYM was significantly higher than that of trichomonads in TPS-1 using Bz-Pro-Phe-Arg-Nan as a substrate. According to the results, it is assumed that the shift from TPS-1 into TYM medium for cultivation of T. vaginalis might modulate the biological and biochemical properties of T vaginalis in vitro.

• Journal of Mushroom
• /
• v.9 no.1
• /
• pp.44-47
• /
• 2011

This study was carried out investigate optimal additive content of spent medium from oyster mushroom in Volvariella volvacea cultivation. The formation of spent mushroom medium from oyster mushroom consisted of 50% pine sawdust, 30% beet pulp and 20% cotton seed hull pellet. The higher amount of spent medium from oyster mushroom resulted in higher total nitrogen content and C/N ratio of medium decreased according to increasing mixing ratio of spent medium from oyster mushroom. The fruit body yield and biological efficiency of supplemented with 50% spent medium from oyster mushroom was same level of non additive medium.

• The Korean Journal of Mycology
• /
• v.36 no.2
• /
• pp.163-171
• /
• 2008

We have surveyed the variation of physical and chemical characteristics of aerobic and anaerobic outdoor fermentation of cotton wastes using for oyster mushroom cultivation. The inner temperature of cotton wastes fermented aerobically covered with thin cloth and setting pallet at bottom was higher than that of anaerobic fermented cotton wastes covered with P.E vinyl and the maximum temperature was $75^{\circ}C$ at 5th day after fermentation. pH of cotton wastes fermented aerobically was increased up to 8.9 after fermentation of $9{\sim}12$ days, but that of anaerobically fermented was decreased up to 5.0. Total carbon content was decreased but total nitrogen content was increased when fermentation was in progress. Oxygen concentration of cotton wastes fermented aerobically was decreased until 6 days after fermentation but increased after 9 days of fermentation. Ammonia concentration of cotton wastes fermented aerobically and anaerobically was below 10 ppm and $20{\sim}85\;ppm$ respectively. In anaerobic condition the cotton wastes was contaminated with mold ($15{\sim}50%$), where no contamination was found in aerobic condition during spawn running stage. Yields of mushroom grown on cotton wastes aerobically fermented for $6{\sim}9$ days was $23.0{\sim}23.6\;kg$ per $3.3\;m^2$ area.

• Horticultural Science & Technology
• /
• v.17 no.3
• /
• pp.355-360
• /
• 1999

This study was carried out to investigate the effect of irrigation frequency, particle size and depth of perlite medium on the growth of Dendranthema grandiflorum (Ramat.) Kitamura 'Shuhouno-chikara' grown on recycling system. In Exp 1, the irrigation frequency (IF) was designed as 3 (IF3), 9 (IF9), and 18 (IF18) times a day. Fine and coarse particle size (PS) of perlite was used as a media, and depth of media (DM) was 15 and 10 cm contained in $34{\times}120{\times}15cm$ styrofoam bed. In Exp 2, the IF was 3 (IF3), 6 (IF6), and 9 (IF9) times a day with the same amount of nutrient solution. Fine and coarse PS were used, and DM was 15 and 7.5 cm. In this study, high IF and fine PS was favorable for plant growth. However, as the IF became frequent, difference of plant growth between fine and coarse PS became smaller. Also, shallow media showed little difference with deep media in plant growth. Thus, decreasing the amount of media was recommended to reduce the production cost.

• Journal of Life Science
• /
• v.17 no.6 s.86
• /
• pp.822-824
• /
• 2007

The efficient system for plant regeneration from cotyledon and primary feat explants of lettuce was established. Plant regeneration efficiency was shown 91.3% from cotyledon and 85.9% from primary leaf explants of variety 'Jungtongpogi' in KN medium. Plant regeneration efficiency was also estimated with various plant regeneration media in variety' Chungchima', which was lowest plant regeneration efficient showing 35.4% from cotyledon and 30.3% from prima leaf explants in KN medium. Kl medium increased 77.9% and 80.7% of plant regeneration efficiencies from cotyledon and primary leaf explants of variety 'Jungtongpogi' were cultured on KN medium. In case of varie쇼 ‘Chungchima', efficient plant regeneration was shown when primary leaf explants were cultured on SH and KI media.

• Journal of Mushroom
• /
• v.16 no.2
• /
• pp.96-102
• /
• 2018

Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high ${\beta}$-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.

• Journal of Bio-Environment Control
• /
• v.29 no.3
• /
• pp.219-230
• /
• 2020

This study was conducted to determine the optimum medium and humidification period for the strawberry (Fragaria × ananassa Duch. cv. Maehyang) cutting seedling production. The cuttings were planted in coir (CO), rockwool (RW), phenolic foam (PF), and organic foam medium (OFM) with fogging treatment in 0, 3, 6, 9, or 12 days on February, 2019. And, as the field verification examination research, cuttings were planted in CO, RW, PF, and OFM, with misting treatment in 0, 6, 9, 12, or 15 days on June, 2019. In fogging treatments, rooting and survival rates of seedlings tended to increase with longer fogging periods, and rooting and survival rates were showed significantly higher in CO medium with more than 9 days of fogging periods. In misting treatments, rooting rate was significantly higher in CO and RW medium with more than 9 days of misting periods, and survival rate was significantly higher with more than 12 days of misting periods. There was no significantly difference by growth medium and humidification period in shoot and root growth. Therefore, when considering the rooting and survival rate the CO medium with 9 days of humidification period could be beneficial for the production of the 'Maehyang' strawberry cutting seedlings.

• Korean Journal of Microbiology
• /
• v.45 no.3
• /
• pp.281-285
• /
• 2009

In this study, we compared the BacT/Alert liquid culture system with Ogawa and $L\ddot{o}wenstein$-Jensen (L-J) media for sputum culture and drug susceptibility test (DST) of Mycobacterium tuberculosis. Rapid liquid culture systems have been widely employed both for primary cultures of M. tuberculosis from clinical specimens and for drug susceptibility test because of its greater sensitivity and faster turn-around time than the conventional egg-based culture methods (Ogawa, $L\ddot{o}wenstein$-Jensen media). Sputum specimens were decontaminated with N-acetyl-L-cysteine (NALC)-4% NaOH and inoculated into the BacT/Alert culture bottles and Ogawa media. 95 from among 135 sputa were smear-positive, 97 (71.9%) were culture-positive by the BacT/Alert culture system, while 89 (65.9%) were positive by Ogawa media. The mean time to culture-positive by the BacT/Alert process system was about 11.3 days, which was significantly shorter than that by Ogawa media (22.4 days). Of 32 M. tuberculosis cultures examined for drug sensitivity, the concordant rate between the two methods (BacT/ Alert liquid culture system, $L\ddot{o}wenstein$-Jensen media) ranged from 87.5% for isoniazid and 90.6% for rifampicin.

• Korean Journal of Microbiology
• /
• v.40 no.1
• /
• pp.37-42
• /
• 2004

To differentiate commercial bifidobacteia for Bifidobacterium lactis Bb-12, Bif. longum Bb-536 and Bif. infantis Maeil-K9, we studied the various carbon source, the nitrogen sources and antibiotics. Amygdalin and fructose were good candidates for carbon sources, and tryptone was suitable for nitrogen sources to design a new selective media for three commercial bifidobacteria. In the case of the amygdalin-containing medium as carbon sources, Bif. lactis Bb-12 and Bif. infantis Maeil-K9 showed good growth, and in fructose-containing medium, Bif. longum Bb-536 showed good growth. In antibiotics resistance study, the addition of 1 mg/L doxycyclin was very effective for differentiation of each bifidobacteria. Doxycyclin did not affect the growth of Bif. lactis Bb-12 and Bif. infantis Maeil-K9, but Bif. longum Bb-536 was completely inhibited by doxycyclin. Finally to confirm the selection capability of newly designed selective media, temperature-shocked bifidobacteria were cultured on them. As the results, fructose or doxycyclin containing medium showed for high growth for temperature-shocked bifidobacteria, but amygdalin containing medium showed low growth of temperature-shocked bifidobacteria.

• The Korean Journal of Mycology
• /
• v.19 no.2
• /
• pp.143-147
• /
• 1991

Botryosphaeria dothidea causing apple fruit rot was cultured in pectin-polypectate mine­raI salts or apple mediumss, to investigate pectolytic enzyme production and activity. Exo-polygalactu­ronase(PG) and exo-polymethylgalacturonase (PMG) in apple medium showed maximum of activity to 6.4 and 7.2 units at six days of culture, respectively. Their maximum activity in pectin-polypectate mineral salts medium was 5.9 and 5.3 units at eight days of culture lower than in apple medium respectively. Endo-PG and endo-PMG in pecin-polypectate mineral salts medium were maximum of activity to 4.4 and 16.2 units at six and eight days of culture, respectively, but activities in apple medium were 3.2 and 6.7 units at eight days of culture. Activity of polygalacturonate-trans-­eliminase(PGTE) and pectinmethyl-trans-eliminase(PMTE) was higher in pectin-polypectate mineral salts medium than in apple medium. Fungal growth was maximum at six and eight days of culture in pectin-polypectate mineral salts and apple medium, respectively.