• Title/Summary/Keyword: 97C9

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Screening for Cytotoxicity of Crude Extracts from Fruit on Leukaemia Cells in Citrus and Related Genera

  • Soo
    • Korean Journal of Plant Resources
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    • v.10 no.3
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    • pp.213-220
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    • 1997
  • The present study has been undertaken to characterize availability of citrus as a medicinal plant with antineoplastic property. The crude extracts from 40 species of fruits with 12 species of the local Citrus in Cheju island were evaluated on their potential activities against mouse P388 lymphocytic leukaemia in vitro. The percent cytotoxicity varied from 25.40 to 97.94% at a concentration of $100{\mu}g/mL$. Among 40 spp., 8 species showed high toxicity more than 90% against P388 cells and Cheongkyool(C. nippokoreana) exhibited the most cytotoxicity as 97.94%($IC_{50}=20.2{\mu}g/mL$). Nine varieties of C. junos were showed insiginicant cytotoxicity. In trifoliate orange, immature fruit was stronger than mature and peel extract showed higher cytotoxicity($IC_{50}=18{\mu}g/mL$) than the other tissues. Hexane fraction from methanol(MeOH) extract of trifoliate orange showed highly significant inhibition of cell growth($IC_{50}=3.9{\mu}g/mL$). In addition, its cytotoxicity increased remarkably from 3.95 to $0.40{\mu}g/mL$ as exposure time legthened. Cytotoxic activities of crude extracts were decreased considerably during a six months storage period. It was apparent that there is considerable variation in cytotoxicity, depending upon species, maturity and storage time of extracts. There was no meaningful cytotoxic difference between archicitrus and metacitrus in the genus Citrus.

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The Crystal Structure of p-Phenylenediamine Dihydrobromide. (p-Phenylenediamine Dihydrobromide의 結晶構造)

  • Choi, Q. Won;Koo, Chung-Hoe;Oh, Joon-Suk;Yoo, Chung-Soo
    • Journal of the Korean Chemical Society
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    • v.9 no.4
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    • pp.174-178
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    • 1965
  • p-Phenylenediamine dihydrobromide and p-phenylenediamine dihydrochloride are found to be isomorphous. p-Phenylenediamine dihydrobromide is triclinic with lattice parameters, $a=4.52{\pm}0.02{\AA}\;b=6.13{\pm}0.02{\AA},c=8.88{\pm}0.03{\AA},\;{\alpha}=111{\pm}1^{\circ},\;{\beta}=97{\pm}1^{\circ},\;{\gamma}=101{\pm}1^{\circ}.$ It belongs to space group $P\bar{1}$, and there is one molecule in the unit cell. The crystal structure is determined according to the method of Fourier synthesis from the electron density projections in three principal crystallographic axes. The crystal structure, thus determined is refined by the method of two-dimensional difference Fourier synthesis.

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Ingestion size of food microalgae of the Pacific oyster Crassostrea gigas larvae (굴, Crassostrea gigas 유생의 먹이생물 섭취 크기)

  • Hur, Young-Baek;Jeon, Chang-Young;Cho, Kee-Chae;Hur, Sung-Bum
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.307-315
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    • 2011
  • Digestibility index of 12 phytoplankton species were invested during the larval development sizes. Ingestible size of phytoplankton varied depending on larval sizes: Isochrysis galbana, I. aff. galbana, Pavlova lutheri, Chlorella ellipsoidea, Nannochloris oculata was ingested 94.2-99.7% all larval sizes. Cheatoceros calcitrans, C. gracilis and C. simplex could ingest over 90.0% after umbo stage (mean shell length $189.3{\pm}13.8{\mu}m$). Phaeodactylum triconutum, Dunaliella tertiolecta and Tetraselmis tetrathele could not ingested D-shaped larvae (shell length $65.0-100.0{\mu}m$) but ingested 97.3-99.7%, 43.3-99.3%, 48.5-99.3% after then larval stages, respectively. But Thalassiosira weissflogii was ingested 1.0-1.7% only at full grown stage. Over 50.0% ingestion cell size was D-shape stage larvae with smaller than mean $102.3{\mu}m$ in shell length could ingest phytoplankton with $4.6{\mu}m$ in both major and minor axis and up to $9.3{\mu}m$ in minor axis basis for larger than mean $158.3{\mu}m$ in shell length, respectively. At all larval stages, phytoplankton with larger than $10.0{\mu}m$ in both major and minor axis could not be ingested.

Review on The Measurement of Wood Shrinkage (목재수축률 측정에 대한 총설)

  • Lee, So Sun;So, Won-Tek;Jeong, Gi Young
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.6
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    • pp.746-756
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    • 2015
  • The goal of this study was to review the wood shrinkage published in Journal of The Korean Wood Science and Technology from 1976 to 2015. Previous studies reported that shrinkage from Larix kaempferi in the tangential, radial, longitudinal directions ranged from 4.21%-9.79%, 2.09%-4.67%, 0.17%-0.33%, respectively. When different drying methods including closed cylinder drying, oven drying, and room temperature drying were used, volumetric shrinkage of Dipterocarpus grandiflorus was different, ranging from 31.6% to 21.0%. With an increment of the drying temperature of $115^{\circ}C$, $120^{\circ}C$, $125^{\circ}C$, the shrinkage of Larix kaempferi did not show a consistent trend. When sample size of Pinus densiflora was increased from $20{\times}20{\times}20mm$ to $100{\times}100{\times}100mm$, the tangential, radial and volumetric shrinkage decreased 2.61%, 1.32%, 0.80%, respectively. When a caliper having a sensitivity of 0.01 mm was used to measure $20mm^3$ specimen from Cryptomeria japonica, the measurement error occurred 1.97% in the radial direction and 35.7% in the longitudinal direction. From the previous studies, wood shrinkage could be influenced by sample size, drying method and measurement technique.

Effects of Water-soaking and Mechanical and Chemical Scarifications on Seed germination of Hairy Vetch (Vicia villosa Roth) (헤어리베치 종자의 침지처리와 종피 파상 및 황산처리가 발아에 미치는 영향)

  • Kim, Min-Tae;Lee, Yong-Hwan;Jeon, Weon-Tai;Kim, Sook Jin;Yun, Da-Hae;Ku, Ja-Hwan;Song, Hana;Lee, Hyeon Bok;Seo, Myung Chul;Kang, Hang-Won
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.49-52
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    • 2013
  • Seed coat of hairy vetch is very thick and hard, and difficult to absorb water during germination. It requires much time that cotyledon comes out from seed coat. Therefore this experiment was carried out to increase the germination rate by enhancing water absorption through water-soaking of seed, chemical scarification by sulfuric acid, and mechanical scarification on seed coat. Water-soaking for 5 hours seemed to be highly effective in enhancing germination rate. Although not significant, water-soaking resulted in increase of germination rate by 9%. Effect of soaking temperature was not significant, but early germination rate was increased to be 5.0%, 31.7% and 48.3% at $10^{\circ}C$, $20^{\circ}C$ and $30^{\circ}C$, respectively. Mechanical scarification of seed coat led to a germination rate of 97% whereas intact seed showed that of 65%. Chemical scarification by sulfuric acid for 10min, 20min and 30min resulted in a germination rate of 76.7%, 74.7% and 96.0% respectively. It is clear that scarification increased germination rate.

Reappearance of the Phase Difference of Omega at Busan (부산지방에서의 오메가위상차의 재현성)

  • Ahn, Jang-Young
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.18 no.2
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    • pp.97-100
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    • 1982
  • The phase differences of the omega were observed continuously for the A-C pair and the A-E pair from Sept. 1, 1980 to Feb. 28, 1981 and from Sept. 20, 1981 to Feb. 82, 1982 at Fisheries University of Busan350$^{\circ}$ 07'.9N, 129$^{\circ}$ 06'.3E)In this paper we deal with the reappearance of these phase differences by annual, month, day As a results, annual eappearance are in good condition both pairs and daily reappearance in day and night are better than an hour around the sunset, and very bad reappearance is found by month.

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Deepest Dormancy Period in Strawberry 'Reiko' and Time for Starting Insulation in Semi-forcing With Lighting in Daegu Area ('여홍' 딸기의 대구지방 휴면최심기와 반촉성 보온개시기)

  • Lee, Woo-Sung;Mun, Jong-Hak;Kim, Seung-Ki;Lee, Ha-Yoon
    • Horticultural Science & Technology
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    • v.19 no.4
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    • pp.488-494
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    • 2001
  • Seedlings of strawberry, 'Reiko' were planted in plastic pots of 24 cm in diameter in the middle of September in 1995, 1996, and 1997. Ten plants were transferred to greenhouse with artificial illumination at 5-day interval from late September to late January. The deepest dormancy of 'Reiko' in Daegu area was found to be on November 5, October 25, and October 31 in 1995, 1996, and 1997, respectively. The time for moving plants to make the plants grow in semi-dormant state in the greenhouse occurred between Nov. 10 and Nov. 20 in both 1995 and 1996, and between Nov. 10 and Nov. 30 in 1997. The time passing approximately 100-200 cumulative hours of exposure to below $5^{\circ}C$ canopy temperature from the peak dormancy, which is required for breaking dormancy of 'Reiko', was between November 16 and 25 in 1996, and November 24 and December 5 in 1997. 'Reiko' yielded high every year when seedlings were moved into greenhouse during the period of October to November. The plants gave good response to forcing culture under lighting. The right time for moving plants into greenhouse in semi-forcing culture appeared to be the time passing 50-150 cumulative hours of exposure to below $5^{\circ}C$ canopy temperature from the peak dormancy.

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A Study on Characteristics of Surface Water and Soil in Wangdungjae Wetland Located at Chiri-Mountain (지리산 왕등재 습지의 지표수 수질 및 토양 환경조사)

  • Kim, Jong-Oh;Lee, Chang-Ho;Ji, In-ju
    • Journal of Wetlands Research
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    • v.3 no.1
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    • pp.61-73
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    • 2001
  • This study was performed to survey the characteristics of surface water and soil in Wangdungjae wetland located at Chiri-Mountain. The results of survey summarized as follows; 1. The physico-chemical characteristics of surface water such as pH, temperature, and DO were in the range of 6.02-6.39, $13.5-24.3^{\circ}C$ and 3.81-9.97 mg/L, respectively. Also, the organic concentrations such as BOD and COD were in the range of 1.3-1.61 mg/L and 3.55-9.97 mg/L, respectively. The water quality of five different sampling sites showed the similar characteristics. 2. The physico- chemical characteristics of soil showed the different properties with the soil sampling depth. According to increasing sampling depth, cation exchange capacity (CEC) and electric conductivity (EC) increased but pH decreased. 3. The future survey and researches on surface water and soil environments are needed to preserve the Wangdungjae wetland at Chiri-mountain marsh.

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Characterization of Tomato spotted wilt virus from Paprika in Korea

  • Choi, Gug-Seoun;Kim, Jeong-Soo;Choi, Jang-Kyung;Kim, Jae-Hyun
    • The Plant Pathology Journal
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    • v.20 no.4
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    • pp.297-301
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    • 2004
  • A Tomato spotted wilt virus (TSWV-KP) was isolated from Paprika (Capsicum annuum var. grossum) showing necrosis spot on the leaves and malformation of the fruit in Yesan, Korea. The virus infected Chenopodium amaranticolor, C. quinoa, Petunia hybrida, Nicotiana glutunosa, Gomphrena globosa, and Physalis floridana. Ten plants including tomato were observed to have systemic TWSV-KP infection. The virus produced necrosis or necrotic ring spots on the inoculated leaves and mosaic, vein necrosis or death on the upper leaves of Datura stramonium, N. clevarandii, N. rustica, and N.tabacum cvs. Thin sections of the infected leaf tissue contained spherical to oval particles, a characteristic of a Tospovirus. The virion contained three molecules of genomic RNAs, which were approximately 9.0, 4.9 and 3.0 kb. The nucleocapsid (N) protein of the purified virion migrated as a single band with molecular weight of about 29 kDa in SDS-PAGE. The N gene of TSWV-KP showed 96.5-97.2% and 97.7-98.5% identities to the three different TSWV isolates of Genbank Database at the nucleotide and amino acid, respectively.

EFFECTS OF PHOSPHORUS AND CALCIUM ON FEED INTAKE AND YIELD AND COMPOSITION OF MILK OF HOLSTEIN COWS

  • Morse, D.;Head, H.H.;Wilcox, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.2
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    • pp.231-237
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    • 1994
  • Three concentrations of P (0.33, 0.43 and 0.54%) and two concentrations of Ca (0.60 and 0.97%) in ration dry matter were evaluated for effects on dry matter intake and on milk yield and composition using 24 Holstein cows. Cows were arranged in a $3{\times}2$ factorial experiment as an incomplete randomized block design with three 28-day periods. Each cow consumed at least one ration with each concentration of Ca. Dry matter intake, yield of 3.5% Fat Corrected Milk, and milk composition were not affected by concentration of P, but milk yield was greater when lowest concentration of P was fed (22.8 vs. 22.1 kg/day; p<0.07). Cows fed rations containing 0.60% Ca had greater milk (22.7 vs. 21.9 kg/day; p<0.02) and 3.5% Fat Corrected Milk yields (p<0.03) and slightly greater protein content than when fed 0.97% Ca. Dietary Ca:P ratios between 1.1:1 and 2.9:1 had no effect on dry matter intake, milk yield, or composition. Concentrations of P in plasma were within the normal range for all rations. Because cows had high dry matter intake, mean daily intakes of both P and Ca were greater than required for their level of milk yield.