• Journal of Aquaculture
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• v.16 no.3
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• pp.135-141
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• 2003

Physiological responses (cortisol, glucose, lactic acid, osmolality and hematology) of olive flounder (Paralichthys olivaceus) to stressors associated with confinement and subsequent transport were investigated. Specimens were subjected to confinement stress for 3 h, prior to transport for 15 h. Two different size cohorts of the fish, large (839.6$\pm$162.7 g) and small (98.2$\pm$14.8 g), were used. Experimental cohorts of the fish were divided into 3 groups for blood sampling: group A, sampled at the beginning of confinement and 3 h before transport (BT, -3 h), after confinement and at the beginning of transport (BT, 0 h), 3 h after transport had begun (AT, 3 h), and after 15 h transport (AT, 15 h); group B, sampled at BT, 0 h, at AT, 3 h, and at AT, 15 h; and, group C, sampled at AT, 3 h, and at AT, 15 h. In the cohort of large fish, plasma cortisol levels of the A group were increased over time, from 4.2 ng/ml (BT,-3 h), to 92.0 ng/ml (BT, 0 h), 118.5 ng/ml (AT, 3 h) and 105.5 ng/ml (AT, 15 h). A similar pattern was evident in the B group, in which cortisol increased from 47.5 ng/ml (BT, 0 h) to 53.5 ng/ml (AT, 15 h); and, for the C group, in which cortisol increased from 43.5 ng/ml (AT, 3 h) to 71.5 ng/ml (AT, 15 h). Glucose levels of the A group also were significantly increased, from 39.5 mg/dl (BT,-3 h), to 121.0 mg/dl (BT, 0 h),298.0 mg/dl (AT, 3 h) and 260.5 mg/dl (AT, 15 h). Lactic acid levels increased markedly during transport, from less than 1 mmol/L (BT, 0 h) to 12.0 mmol/L (AT, 15 h). Plasma osmolality increased from 405.5 mOsm/kg (BT, -3 h, for group A) to values near 500 mOsM/kg subsequent to confinement and transport. In the small-size cohort, plasma cortisol, glucose, lactic acid and osmolality levels showed similar but less pronounced trends than those observed for the large-size cohort. This research provides baseline data on cortisol, glucose, lactic acid, osmolality and hematological responses to confinement and transport, which should be useful to aquaculturists working with olive flounder and to scientists studying other flatfish species.

• Journal of Veterinary Clinics
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• v.14 no.2
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• pp.201-207
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• 1997

This study was carried out to investigate the changes of blood chemical values in 61 crossbred calves at 1 day, 1 week, 2 weeks, 4 weeks and 9 weeks of age, respectively. The blood chemical values of calves with diarrhea were compared with those of normal calves, and blood chemical values were compared for groups of calves with weight gain divided into four ranges; up to 0.9 kg/wk and over 7.0 kg/wk. The results obtained in this study show that the mean values and standard deviation of red blood cell (RBC) and packed cell volume (PCV) of normal calves at 1 day of age (806.7$\pm $56.5 10$^{4}$/$\mu$l and 33.8$\pm $5.2 %) decreased at 1 week of age and increased at 2 and 4 weeks of age. The PCV of normal calves at 9 weeks of age (32.6$\pm $2.3 %) was significantly lower than that at 2 and 4 weeks of age (p<0.05). Total protein of normal calves at 1 day of age (6.8$\pm $0.5 g/100 ml) decreased to 6.4$\pm $0.6 g/100ml at 1 week and increased to 7.0$\pm $ 0.7 g/100 ml at 2 weeks. After 2 weeks of age, total protein tended to decrease gradually to 9 weeks of age. Glucose of normal calves at 9 weeks of age (78$\pm $4 mg/100 ml) was significantly lower than that at 1 week (110$\pm $8 mg/100 ml) (p<0.01). At all ages, RBC, white blood cell (WBC), PCV and fibrinogen of calves with diarrhea were higher than those of normal calves. RBC and PCV of calves with diarrhea at 1 week of age (929.7$\pm $39.7 10$^{4}$/$\mu$ and 42.3$\pm $0.4 % were significantly higher than those of normal calves at 1 week (786.4$\pm $80.9 10$^{4}$/$\mu$l and 32.2$\pm $3.8 %) (p< 0.05), which suggest that diarrhea at this age can be dangerous. The weekly weight gain was an average of 4.2 kg/wk from birth to 9weeks. RBC, PCV, fibrinogen, total protein and vitamin E of calves with weight gain more than 4.0kg/wk. From the above results we obtained eledata support the importance of providing high quality feed from 4 weeks to 9 weeks of age for satisfactory weight gain of calves.

• Korean Journal of Veterinary Research
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• v.46 no.1
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• pp.7-12
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• 2006

We have studied pharmacokinetics of a new anti-human immunodeficiency virus (HIV) agent VP-0501 and its amino acid prodrug VP-0501AL which is designed to improve oral bioavailability. After oral administration at 100 mg/kg dose in rats (n = 4), VP-0501 was not detectable in plasma (<50 ng/ml), while after the administration of VP-0501AL, VP-0501 was quantitatively detected, at least for 8 hrs, with Cmax of ca. $2.5{\mu}g/ml$ and AUC of $8hr^{\ast}{\mu}g/ml$. When VP-0501 was intravenously administered at 50mg/kg, this compound appeared at a marginal level in plasma with AUC of $2hr^{\ast}{\mu}g/ml$, $t_{1/2}$ of 2 hr, $C_0$ of $0.7{\mu}g/ml$, and MRT of 3 hr. On the other hand, with intravenous VP-0501AL at the same dose, both the prodrug VP-0501AL and its metabolite VP-0501 appeared comparatively at higher level in the plasma: pharmacokinetic parameters of VP-0501AL including $Vd_{\beta}$, AUC, $t_{1/2,{\beta}}$, $C_0$, $CL_{tot}$, and MRT were ca. 2 L/kg, $70hr^{\ast}{\mu}g/ml$, 2 hr, $180{\mu}g/ml$, 0.7 L/hr/kg, and 1 hr, respectively. These results demonstrate that attachment of amino acid alanine to VP-0501 is an effective approach for improvement of its oral bioavailability. Therefore, VP-0501AL is expected to become a new highly bioavailable and potent anti-AIDS drug candidate/lead compound.

• The Korean Journal of Physiology
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• v.2 no.2
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• pp.11-20
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• 1968

Maximal oxygen uptake was measured in thirty-three secondary school girls by means of the treadmill test. Eighteen middle school girls aged 14.0 (range: $13.0{\sim}15.9$) years and fifteen high school girls aged 16.9 (range: $16.0{\sim}18.0$) years served as subjects. Maximal treadmill run lasted for 2 minutes and 20 seconds and the expired air was collected in a Douglas bag through a J-valve during the last one minute period. In general, absolute values of various measurements in the high school girls were greater than those of the middle school girls. When values were expressed on the body weight or lean body weight basis, however, work capacity of middle school girls was superior to that of the high school girls. The detailed results are as follows: 1. In middle school girls maximal oxygen uptake was 1.78 l/min., 47.4 ml/kg body weight, 12.3 ml/cm body height, and 61.7ml/kg lean body mass. In high school girls maximal oxygen uptake was 1.93 l/min., 39.7ml/kg body weight, 12.3 ml/cm body height, and 51.2 ml/kg LBM. Although the absolute value of maximal oxygen uptake was greater in high school girls than in middle school girls, values expressed on the body weight basis showed the reverse trend, namely, values of the middle school girls was greater than those of the high school girls. 2. The ratio of maximal to resting oxygen uptake was 8.8 in the middle school girls and was 10.2 in the high school girls. 3. Maximal pulmonary ventilation in the middle school girls was 55.3 l/min. and 66.1 l/min. in the high school girls. The ratio of maximal to resting pulmonary ventilation was 10.2 in the middle school girls and 10.1 in the high school girls. 4. The correlation between body weight and maximal oxygen uptake was relatively high, namely, r=0.79 both in middle and high school girls. The correlation coefficient between body weight and maximal pulmonary ventilation was a little less that of between maximal oxygen uptake and showed a value of r=0.60 both in middle and high school girls. The lean body mass was a poor reference of maximal oxygen uptake or maximal pulmonary ventilation as compared to body weight. The correlation between maximal oxygen uptake and maximal pulmonary ventilation was high and the coefficient of correlation in middle school girls was 0.927 and in high school girls it was 0.856. 5. Maximal ventilation equivalent was 30.9 liters in middle school girls and 33.9 liters in high school girls. This indicated that no hyperventilation was induced during the maximal of oxygen uptake exercise period as related to the maximal oxygen uptake. 6. Heart rate reached to the peak value within 1.5 minutes after beginning of maximal oxygen uptake run and remained at the same peak plateau level throughout the entire running period. Heart rate decreased steeply on cessation of running and subsided slowly thereafter. The maximal heart rate was 184 beat/min. in middle school girls and 189 beat/min. in high school girls. 7. Maximal oxygen pulse was 9.4 in middle school girls and 9.9 ml/beat in high school girls.

• The Korean Journal of Physiology
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• v.2 no.2
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• pp.1-10
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• 1968

Maximal oxygen consumption measurements were performed on 15 middle school boys (age: mean 14.0, range: $13{\sim}16$ years) and 14 high school boys (age: mean 17.4, range: $16{\sim}19$ years). General body build was greater in the high school boys and absolute values of body height, body weight, skinfold thicknesses, maximal oxygen uptake, and maximal pulmonary ventilation followed the same trend. Considered on the basis of body build, however, the values of high school boys were not always greater than those of middle school boys. The following results were obtained. 1. Maximal oxygen consumption in middle school boys was 2.11 l/min., 53.7ml/kg b. weight, 13.9 ml/cm body height, and 63.7 ml/kg LBM. In high school boys the values were: 2.86 l/min., 52.7 ml/kg b.wt., 17.5 ml/cm b. height, and 57.9 ml/kg LBM. Thus, middle school boys were superior to high school boys on body weight and lean body mass basis. They were also superior to the European boys of the same age. 2. The ratio of maximal oxygen uptake to resting value was 9.7 in middle school boys, and 10.8 in high school boys. 3. Maximal pulmonary ventilation in middle school boys was 58.0 l/min., and 84.0 l/min. in high school boys. The ratio of maximal ventilation to resting value was the same as oxygen uptake, namely, 9.7 in middle school boys and 10.7 in high school boys. 4. Ventilation equivalent in middle school boys was 27.5 and 29.3 in high school boys. These values represent values of untrained male subjects. 5. Maximal heart rate in high school boys reached to 193 beat/min. and is 2.9 times that of resting heart rate. 6. Maximal oxygen pulse in high school boys was 16.6 ml/beat and was same as that of untrained subject. 7. Correlation between body weight and maximal oxygen consumption in middle school boys was r=0.570, and r=0.162 in high school boys. Correlation between lean body mass in middle school boys was r=0.499, and r=0.158 in high school boys. Interrelation between body weight and maximal pulmonary ventilation was poor. 8. The differences between trained and untrained subjects were discussed.

• The Korean Journal of Pharmacology
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• v.24 no.1
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• pp.125-134
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• 1988

A single i.v. dose of streptozotocin (65 mg/kg) given to rats has produced a marked hyper-glycemia (>500 mg serum glucose/dl). Since the Atractylodis Rhizoma is known to have hypo-glycemic action, the water extracts of Atractylodis Rhizoma (ARWE) was given to the streptozotocin-induced (SZ) hyperglycemic rats. To determine whether ARWE has the anti-hyperglycemic effects, two different daily doses of ARWE (i.e.0.2 g/kg and 2.0 g/kg) were given orally to the SZ rats for up to 8 days. Thereupon, serum levels of glucose, insulin, amylase and cholesterol were determined on days 1, 3 and 8, following the initial and repeated daily administrations of ARWE. On day 8, glycogen content and glucose-6-phosphatase activity in the liver were assayed. Results showed that ARWE decreased the serum glucose levels, which had been markedly elevated by the SZ pre-treatment. In support of this, the serum insulin level, which had been quickly lowered by the SZ pre-treatment $(20{\mu}U/ml)$, was quickly elevated in the ARWE dose dependent manner that, at 2.0 g/kg ARWE, the serum insulin level was increased $(20{\mu}U/ml)$ above the normal level $(42{\mu}U/ml)$. Also, the serum amylase level, which was steadily decreasing after the SZ pre-treatment, was restored to the normal level folowing 8 day of ARWE (2.0 g/kg) treatment. Hepatic glycogen content and glucose-6-phosphatase activity, which decreased and increased, respectively in the 52 treatment group, were restored toward the normal level in SZ plus ARWE group.

• Journal of Animal Science and Technology
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• v.44 no.1
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• pp.69-74
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• 2002

This study was conducted to investigate the milk quality produced from dairy farms in Jeju province and to analyze the variety of pathogenes and the number of somatic cells in the milk. Data were collected from 262 lactating cows from 8 farms of three regions and the results obtained are summarized as follows. 1. The average daily milk yields and milk fat contents from June and October were 22.3 kg and 3.7%, respectively. 2. The average number of bacterial counts in raw milk was 1.5${\times}10^4$/ml, but that in farm A was over 3.0${\times}10^4$/ml. 3. The somatic cell counts were 2.5${\times}10^5$/ml in average and those in farm G and H were higher than those in other farms. Their distribution in 262 lactating cows from June to October was as follows; less than 2.0${\times}10^4$ /ml in 68.8% of cows, 2.0-5.0${\times}10^4$/ml in 18.8% of cows and more than 5.0${\times}10^4$/ml in 12.4% of cows. 4. Of the 113 isolates (compartments of cow udder) from mastitic milk, Staphylococcus aureus was found in 47, Streptococcus ogalatiae in 17 and Bacillus in 12. 5. The average monthly income of the farmers was 407 thousand won/head, and that in farm A or E was higher than that of farm H (456-475 thousand won vs. 314 thousand won) In conclusion, to improve income dairy farmers should reduce the somatic cell counts in the milk and mastitis infection through regular disinfection and inspection.

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.291-299
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• 1997

In order to establish optimal dosage schedules and withdrawal times for sulfamethazine(SMZ) in pigs, pharmacokinetic and tissue distribution experiments were conducted in pigs. For comparative purposes, tissue depletion kinetics are also studied in rats. From three pigs administered with SMZ i.v., the pharmacokinetic profile of SMZ in two pigs was adequately described by a one-compartment open model whereas that in one pig was patterned after a two-compartment open model. Volume of distribution(Vd) was 0.48~0.57 L/kg and biological half-life($t_{1/2}$) was 11.8-16.8 h. From three pigs dosed with SMZ p.o., pharmacokinetic profile was explainable with a one-compartment open model. Time to reach maximum SMZ concentration in serum (Tmax) was 2.8 h, 3.2 h and 7.5 h. Elimination half-life was 2.8-7.5 h. The descending order in concentration of SMZ was plsama > kidney > liver > lung > heart > pancreas > spleen > duodenum > ileum > brain > adipsoe tissue from three pigs sacrificed at 5h, 29h and 54h after the administration of SMZ, p.o.. The protein binding of SMZ in pigs was 55.2%($2.5{\mu}g/ml$), 71.5% ($5{\mu}g/kg$) and 71.5%($10{\mu}g/ml$). The mean systemic bioavailability (F) of SMZ p.o. was 49.1 %. Meanwhile the pharmacokinetic profile of SMZ in rats was adequately described by a one-compartment open model. Absorption of SMZ p.o. in the rat was very rapid. In conclusion, the oral optimal dosage regimen of SMZ for pigs was the initial dose of 45.7 mg/kg followed by the maintenance dose of 30.2 mg/kg for high specific pathogens to SMZ. The time to reach below the stipulated residual allowable concentration (0.1 ppm) was calculated 93 h after oral administration of 200 mg/kg recommended by manufactureres.

• The Korean Journal of Physiology
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• v.1 no.1
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• pp.91-101
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• 1967

The maximal oxygen intake (MOI) was determined in 54 males (23 nonathletes, 10 basketball players, 8 hand ball players, 7 weight lifters and 6 long distance runners) and in 49 females (24 non-athletes, 16 basketball players and 9 volley ball players) by using a treadmill method outlined in Part I of the present investigation and the following results were obtained: (1) The maximal aerobic work capacity varied from the lowest value of 1,008 kg-m/min in female non-athletes to the highest value of approximately 2,000 kg-m/min in male basketball players and long distance runners. Values of other groups varied from 1,400 kg-m/min in male non-athletes and female athletes to 1,800 kg-m/min in male hand ball players. (2) The MOI per unit body weight varied from the lowest value of 41.3 ml/min/kg in female non-athletes to the highest value of 67.6 ml/min/kg in male long distance runners. Values of other groups were in the order of 47 to 55 ml/min/kg. (3) The heart rate during maximal aerobic work performance varied from the lowest value of approximately 180 per min in female basketball players and male long distance runners to the highest value of 190 or above in nonathletes of both sexes. (4) An estimate of oxygen debt as measured during 15 minutes following the maximal work was 3.841 in female non-athletes, 4.681 in female athletes, 5.561 in male non-athletes and 6.321 in male athletes. These results indicate that the MOI per unit body weight as well as the oxygen debt of Korean non-athletes were comparable to, while corresponding values of Korean athletes were considerably lower than, those of other countries such as Japan, the United States of America and Europe.

• Proceedings of the ESK Conference
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• 1997.04a
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• pp.39-49
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• 1997

The objective of this study was to make comparison of the psychophysical MAWLs for different manual materials handling tasks. Lifting activities with four different lifting frequencies (2, 5, 8, 11 lifts/min) for one lifting range from floor to 76cm height were studied. The oxygen consumption rate and heart rate were measured or recorded while subjects were lifting their MAWLs. Psychophysical MAWL decreased from 22.38 kg to 7.48 kg, while th oxygen consumption rate with the MAWL increased from 717.8 ml/min to 1114.7 ml/min as the frequency increased from 2 to 11 Lifts/min. Heart rate also increased from 104.5 to 120.7 bpm. It was found that the relationship between MAWL and frequency can be described best by the exponentaial function with the R-sq value 0.9865 for this study. The percent ratio of oxygen consumption rate with the MAWL and PWC ranged from 28% to 43%.