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Characterisation of fungal contamination sources for use in quality management of cheese production farms in Korea

  • Kandasamy, Sujatha;Park, Won Seo;Yoo, Jayeon;Yun, Jeonghee;Kang, Han Byul;Seol, Kuk-Hwan;Oh, Mi-Hwa;Ham, Jun Sang
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.6
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    • pp.1002-1011
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    • 2020
  • Objective: This study was conducted to determine the composition and diversity of the fungal flora at various control points in cheese ripening rooms of 10 dairy farms from six different provinces in the Republic of Korea. Methods: Floor, wall, cheese board, room air, cheese rind and core were sampled from cheese ripening rooms of ten different dairy farms. The molds were enumerated using YM petrifilm, while isolation was done on yeast extract glucose chloramphenicol agar plates. Morphologically distinct isolates were identified using sequencing of internal transcribed spacer region. Results: The fungal counts in 8 out of 10 dairy farms were out of acceptable range, as per hazard analysis critical control point regulation. A total of 986 fungal isolates identified and assigned to the phyla Ascomycota (14 genera) and Basidiomycota (3 genera). Of these Penicillium, Aspergillus, and Cladosporium were the most diverse and predominant. The cheese ripening rooms was overrepresented in 9 farms by Penicillium (76%), while Aspergillus in a single farm. Among 39 species, the prominent members were Penicillium commune, P. oxalicum, P. echinulatum, and Aspergillus versicolor. Most of the mold species detected on surfaces were the same found in the indoor air of cheese ripening rooms. Conclusion: The environment of cheese ripening rooms persuades a favourable niche for mold growth. The fungal diversity in the dairy farms were greatly influenced by several factors (exterior atmosphere, working personnel etc.,) and their proportion varied from one to another. Proper management of hygienic and production practices and air filtration system would be effective to eradicate contamination in cheese processing industries.

The flora of vascular plants in Gibaeksan Mt. County Park and mountains neighboring the park (기백산군립공원과 인접산지의 관속식물상)

  • PARK, Beom Kyun;SON, Dong Chan;KO, Sung Chul
    • Korean Journal of Plant Taxonomy
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    • v.50 no.2
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    • pp.166-198
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    • 2020
  • The flora of vascular plants in the Gibaeksan Mt. County Park and its neighboring mountains, located at the boundary between Geochang-gun and Hamyang-gun in Gyeongsangnam-do province in Korea, were surveyed for a total 46 times from April to September of 2011, in July of 2012, and from April of 2015 to August of 2018. The result of this survey revealed 659 taxa composed of 107 families, 346 genera, 583 species, 14 subspecies, 46 varieties and 6 forms. Among them, 25 taxa were endemic plants to Korea, and 18 taxa were rare and endangered plants of Korea. The floristic regional indicator plants including cultivated plants were 5 taxa of grade V, 5 taxa of grade IV, 29 taxa of grade III, 30 taxa of grade II and 38 taxa of grade I. Forty-three taxa of alien plants were found in this area. In addition, 500 taxa out of a total of 649 taxa were categorized by usage into eight groups, including among others an edible group containing 257 taxa, a medicinal group containing 206, a pasturing group containing 220, and an ornamental group containing 84, with some taxa belonging to more than one group. The flora of this surveyed area belongs to the southern province among the floral provinces of the Korean Peninsula.

A Survey on the Current Status of Safety and Health and of Safety Management Levels among Korean Native Cattle Farms (한우 농가의 농작업 안전보건 실태 및 안전관리 수준 조사)

  • Kim, Insoo;Lee, Kyung-Suk;Kim, Hyo-Cher;Chae, Hye-Seon;Kim, Kyungsu;Choi, Dong-Phil
    • Journal of Environmental Health Sciences
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    • v.43 no.1
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    • pp.42-54
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    • 2017
  • Objectives: The present study was conducted to investigate farm work environments among farmers and examine the level of management of safety and health, and to subsequently produce study result to serve as foundational data for the development of guidelines on safety and health as part of the improvement of farming work environments among farmers raising Korean native cattle. Methods: The present study conducted a survey on farm work environments and the management of safety and health with 407 farmers engaged in Korean native cattle farming in selected regions in eight provinces. It also visited 10 farmers to verify the current status of farm work. Results: The survey results showed that 16.4% of the respondents experienced safety-related accidents due to farm work. The locations of the accidents were inside the cattle shed (71.4%) and facilities outside the cattle shed (19.6%). The types of accident showed collision with animals (35.7%), collision or contact with obstacles (27.1%), and musculoskeletal accidents due to heavy object handling (12.9%). The causes of the accidents were cattle (38.3%), cultivators and tractors (25.4%), facility tools in cattle sheds (9.0%), and slippery floors (6.0%). The damaged areas were hand (21.0%), spine (19.8%), lower limb (18.5%), and foot (17.3%). A self-diagnostic survey on respiratory diseases showed that 11.5% of the respondents experienced respiratory-related symptoms. The survey on safety and health during farm work showed that wearing personal protective equipment and response to emergency situations, which were needed to prevent safety-related accidents, were relatively low compared to the level of recognition of the need and awareness of safety issues. Furthermore, the field survey identified the current status of safety and health issues such as prevention management of collision accidents with cattle, how to handle heavy objects, and wearing of personal protective equipment. Conclusions: The present study identified safety-related accidents and problems in the management of safety and health among Korean native cattle farmers. In order to address the problem, it is necessary to not only provide guidelines on safety and health management which are appropriate to the characteristics of Korean native cattle farming work, but also to study the development of personal protective equipment.

The Relationship Between of Marriage Migrant Women's Oral Health Belief and Self-Efficacy on Oral Health Awareness (결혼이주여성의 구강건강신념 및 자기효능감과 구강건강인식도와의 관련성)

  • Ka, Kyung-Hwan;Moon, Seon-Jeong;Ku, In-Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.12
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    • pp.8487-8497
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    • 2015
  • The purpose of this study was to determine the relationship between of oral health belief and self-efficacy on oral health awareness for marriage migrant women's in the Yeongnam region and provide basic data that could help develop programs necessary to improve oral health awareness and change attitudes; for this purpose, a self-administered questionnaire based on interview was used in marriage migrant women's using eight multicultural centers in Daegu and North and South Gyeongsang Provinces from October 1 to December 15, 2013 and 256 copies were finally analyzed. The statistically analysis was performed using SPSS 18.0 and AMOS 7.0, with the statistical significance level set at p<.05. As for the effects of oral health belief and self-efficacy on oral health awareness, the experience of scaling was affected by seriousness(${\beta}$=.568) among the factors of oral health belief(p<.05), oral health status was affected by sensitivity(${\beta}$=-.391)(p<.01), and oral health concern was affected by sensitivity(${\beta}$=-.183), seriousness(${\beta}$=.172), usefulness (${\beta}$=.224), and self-efficacy(${\beta}$=.237)(p<.01). It is necessary to analyze the effects of oral health belief and self-efficacy on oral health awareness, make positive efforts to develop preventive oral health management and oral health education programs, and make fundamental policies for improving oral health in multicultural families so that marriage migrant women's can make efficient oral health management.

Analysis of Consumers' Present Use and Future Demand of Traditional Korean Liquors (전통주에 대한 소비자의 이용 현황 및 요구도 분석)

  • Kim, Ji-Young;Park, Geum-Soon
    • Korean journal of food and cookery science
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    • v.30 no.1
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    • pp.41-50
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    • 2014
  • The purpose of this research was to exam the recognition and use for traditional Korean liquors and to consider the problems of traditional liquor businesses and the plans for its popularization. This study was conducted with 411 adults of 20 years old and above, all of whom lived in the Daegu and Gyeongbuk Provinces. According to the survey, females were higher than males(49.6%) by 50.4%, and ages 20 was the highest. With regards to what kind of liquor they enjoyed, the consumers enjoyed soju the most, followed by beer and traditional Korean liquors. The favorite liquor of the men was soju, followed by beer and traditional Korean liquor, and the women mostly liked beer, followed by soju and wine. An important-performance analysis (IPA) was performed for 17 attributes of traditional Korean liquor and identified the targets for product management strategies, including 'Variety', 'Functionality', 'Extended Recognition' and 'Healing hangovers'. The recognition of traditional Korean liquor was high in the order of takju, Fruit wine, Chongju, Yakju, Distilled soju, and Distilled liquor. As a result, the developing solid concepts of marketing strategy are required and may be achieved by understanding the consumer preferences and demands of traditional Korean liquors.

Occurrence of Pome Fruit Viruses on Pear Trees (Pyrus pyrifolia) in Korea (국내에서 발생하는 배나무 바이러스병)

  • Cho, In-Sook;Kim, Dae-Huyn;Kim, Hyun-Ran;Chung, Bong-Nam;Cho, Jeom-Doeg;Choi, Gug-Seoun
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.326-330
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    • 2010
  • Three pome fruit viruses, Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASPV) and Apple stem pitting virus (ASGV) were detected in pear trees (Pyrus pyrifolia) using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in Ansung, Naju and Ulsan provinces of Korea. Infection rate of three viruses was 35.2% from 452 leaf samples of the three cultivars of pear trees. Also, each of three viruses was detected by reverse transcription polymerase chain reaction (RT-PCR) for a limited number of samples. Infection rate of three viruses was 86.3% from 233 leaf samples of the three pear cultivars. The virus infection rates by RT-PCR were much higher than ELISA. ASGV was prevailing on pear with 74.2%, whereas ASPV and ACLSV were found in 34.8% and 0.4% of tested samples, respectively. Symptoms caused by ASGV showed black spots of infected Niitaka cultivar leaves. The ACLSV, ASPV and ASGV isolates showed 83~94% sequence identity at a nucleotide level to other pome fruit virus isolates when analyzed by NCBI BLAST. Pome fruit viruses occurring in pear were ACLSV, ASPV and ASGV. This is the first report of pear trees infected ASPV in Korea.

Traits of Agro-meteorological Disasters in 20th Century Korea (20세기 한국의 농업기상재해 특징)

  • 심교문;이정택;이양수;김건엽
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.4
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    • pp.255-260
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    • 2003
  • Recently, both climate change and unusual meteorological disasters are becoming a more frequent and serious threat to agricultural production. Destruction of the stabilizing base of agricultural productivity in Korea is a concern. This study provides basic information for stabilizing agricultural production by clarifying and analyzing the features of agro-meteorological disasters which have occurred recently in Korea. The occurrence of meteorological disasters has increased rapidly since the 1940s. A 19-fold increase in occurrence is noted over the past 60 years from 1941 to 2000. Meteorological disasters occurred mostly in August, then in July, and least often in October, In terms of regional occurrences, the frequency of meteorological disasters was the highest in Gangwon (751 times) and in Jeonnam (703 times) provinces, and the lowest in Jeju (459 times) province for the 97 years from 1904 to 2000. Agro-meteorological disasters which caused the most serious damage to cropland were rain storms and typhoons for the 10 years from 1991 to 2000, and they occurred 52 and 18 times during this period, respectively. Agro-meteorological disasters occurred mainly during the summer season (from June to September) when major crops are cultivated in Korea.

A Comparison Study on Satisfaction with Motivation-Hygiene Factors between Technology and Other Teachers in Gwangju City and Chon-nam Province (광주.전남 지역의 중등학교 기술 담당 교사의 동기-위생요인 간 만족도 및 타 교과 교사와의 비교 연구)

  • Jang, Nae-Chan;Kim, Seong-Il
    • 대한공업교육학회지
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    • v.34 no.2
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    • pp.230-247
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    • 2009
  • This study was performed to investigate and analyze technology teachers' and other subject teachers' satisfactions with motivation-hygiene factors in order to provide informations for their job motivation at secondary schools. The data were collected from 216 technology teachers and other subject teachers in Gwangju City and Chon-nam Province and analyzed and tested at p<.05 or more by employing t-test through SPSS program. The average satisfaction levels of the teachers were M = 3.85 with compounded motivation factors and M = 3.38 with compounded hygiene factors. The former is higher the latter. In the satisfaction levels of individual factors responsibility was first, work itself second, achievement third, and human relation, job safety, growth opportunity, recognition, supervision skill subsequently went down. The satisfaction levels of these factors were higher than neutral(M = 3.00). On the other hand, the satisfaction levels of work environment and payment were a little lower than neutral. Technology teachers were more satisfied than other subject teachers with responsibility and human relation factors among 10 motivation and hygiene factors. They had more positive perception than the others with these two. Generally, these teachers groups presented almost the equal satisfaction level with motivation and hygiene factors. The data were collected from technology teachers and other subject teachers in Gwangju City and Chon-nam Province and thus these might not be applicable to teachers in other cities and provinces.

Isolation and Development of Quantification Method for Cyanidin-3-Glucoside and Cyanidin-3-Rutinoside in Mulberry Fruit by High-Performance Countercurrent Chromatography and High-Performance Liquid Chromatography

  • Choi, Soo-Jung;Jeon, Heejin;Lee, Chang Uk;Yoon, Shin Hee;Bae, Soo Kyung;Chin, Young-Won;Yoon, Kee Dong
    • Natural Product Sciences
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    • v.21 no.1
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    • pp.20-24
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    • 2015
  • Cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside (C3R) were isolated by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (1 : 3 : 1 : 5 : 0.01, v/v) to give pure C3G (34.1 mg) and C3R (14.3 mg) from 1.5 g crude mulberry fruit extract. Using the pure C3G and C3R, a reliable high-performance liquid chromatography (HPLC) method was developed and validated to determine the C3G and C3R contents in mulberry fruit. C3G and C3R were separated simultaneously using an Eclipse XDB-C18 column ($4.6{\times}250mm$ I.D., $5{\mu}m$) coupled with a photodiode array detector (PDA). The gradient elution of the mobile phase consisting of acetonitrile (0.5% formic acid) and water (0.5% formic acid) was applied (1.0 mL/min), and the detection wavelength was 520 nm. The calibration curves of C3G and C3R showed good linearity (both with $r^2=0.9996$) in the concentration range $15.625-500{\mu}g/mL$, and the relative standard deviations (RSD%) of intra- and inter-day variability were in the ranges 2.1 - 8.2% and 4.1 - 17.1%, respectively. The accuracies were ranged 96.5 - 102.6% for C3G and C3R, respectively. The developed HPLC method was used to determine the contents of C3G and C3R in newly harvested mulberry from eight different provinces of Korea.

Analysis on Conflict Minerals and Its U.S. Policy (분쟁광물과 미국의 관련 정책분석)

  • Park, Sung-Won;Kim, Seong-Yong;Kim, You-Dong
    • Economic and Environmental Geology
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    • v.47 no.3
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    • pp.255-263
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    • 2014
  • Conflict minerals refer to minerals mined in conditions of armed conflict, especially as in the eastern provinces of the Democratic Republic of the Congo. The common conflict minerals are cassiterite, wolframite, coltan(columbite-tantalite ore), and gold, which are mined and extracted from the Eastern Congo. These minerals are essentially used in the manufacture of a variety of devices, including consumer electronics. To end the violent conflict in the Democratic Republic of the Congo (DRC) and in surrounding countries, it is necessary to block the supply route of conflict minerals which has been partially financed by the exploitation and trade of conflict minerals. The Dodd-Frank Wall Street Reform and Consumer Protection Act, passed into law in July 2010 and it contains requirements that U.S. companies report to the Securities and Exchange Commission(SEC) on the origin of conflict minerals and show due diligence of OECD. The goal of the act is to cut direct and indirect funding of armed groups engaged in conflict.