• Journal of Korean Physical Therapy Science
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• v.7 no.2
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• pp.523-531
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• 2000

The purpose of this study was to investigate the Effect of stimulus in the Acupuncture point with Ultrasound and Electro-acupuncture on human's Cholinesterase activity. Pain is physiological symptoms in human body. Pain stimulation transference is according to Acetylcholine that is a matter of nerve stimulation. Cholinesterase analyze the Acetylcholine and Cholin acetylase compose the Acetylcholine. To identify of Cholinesterase activity, this study attempted to Electroacupuncture and Ultrasound for 20 person. Twenty subjects participating in this consisted of A group(difference of Cholinesterase's figure make use of Electroacupuncture with Zi-Shil), B group(difference of Cholinesterase's figure make use of Ultrasound with Zi-shil), C group(difference of Cholinesterase's figure make use of Eltroacupuncture and Ultrasound with Zi-shil). As a result the following conclusion was draun: 1. A group showed significantly decreased of Cholinesterase's figure make used of Electroacupuncture with Zi-Shil(the period is between pre-application and 20minute after application, between pre-application and mean of 20minute-30minute after application). 2. B group showed significantly decreased of Cholinesterase's figure make used of Ultrasound with Zi-Shil(the period is between pre-application and 3minute after application, between 3minute after application and 7minute after application, between preapplication and 7minute after application, between pre application and mean of 3minute-7minute after application). 3. C group not showed significantly decreased of Cholinesterase's figure make used of Elcetroacupuncture and Ultrasound with Zi-Shil(the period is between pre-application, between 20minute-Eletroacupuncture and 3minute-Ultrasound after application, between 30minute-Eletroacupuncture and 7minute-Ultrasound after application). The results of this study showed that decrease of Cholinestese' s figure make used of Elctroacupuncture and Ultrasound Zi-Shil. The result mean stimulation of Elctroacupuncture and Ultrasound decreased Acetylcholin' quantity. And decrease of Acetylcholin' quantity decreased Cholinesterase' figure.

• YAKHAK HOEJI
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• v.46 no.5
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• pp.313-319
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• 2002

Synthesis of 7$\beta$-[(Z)-2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxyimino)acetamido]-3-[[(3S, 5S)-5-[4-phenyl-5-(4-methylphenyl or 2-thiophenyl)-4H-l, 2, 4- triazol-3-yl]thiomethylpyrrolidin-3-yl]]thiomethyl-3-cephem-4-carboxylic acids (7a, 7b) were described. (2S, 4S)-4-acethylthio-2-[4-phenyl-5-(4-methylphenyl or 2-thiophenyl)-4 H-1, 2, 4-triazol-3-yl]thiomethyl-1-tert-butoxycarbonylpyrrolidines (4a, 4b) were prepared from trans-4-hydroxy-L-proline with (2S, 4R)-absolute configuration as starting material. 4-Phenyl-5-(4-methylphenyl or 2-thiophenyl)-4 H-l, 2, 4-triazol-3-thiols (2a, 2b) were prepared from p-toluic anhydride and 2-thiophene carboxylic acid hydrazide, respectively. p-Methoxybenzyl 7$\beta$-(Z)-2-(2-for-mamidothiazol-4-yl)-2-(1-tert-butoxycarbonylisopropylimino]acetamido-3-[[ (3S, 5S)-5-[4-phenyl-5-(4-methylphenyl or 2-thio phenyl)-4H-1, 2, 3-triazol-3-yl]thiomethyl-1- tert-butoxycarbonylpyrrolidin-3-yl]]thiomethyl-3-cephem-4-carboxylates (6a, 6b) were achieved by using p-methoxybenzyl ]7P-(Z)-2-(2-formamidothiazol-4-yl)-2-(tert-butoxycarbonylisopropylimino] acetamido-3-chloromethyl-3-cephem-4-carboxylate (5) and (2S, 4S)-4-acethylthio-2-[4-phenyl-5-(4-methyl phenyl or 2-thiophenyl)-4H-1, 2, 4-triazol-3-yl]thiomethyl-1-tert-butoxycarbonyl pyrrolidines (4a, 4b). Removal of formyl, Boc, and p-methoxybenzyl protecting groups were carried out by triflu oroacetic acid and anisole to give the target compounds.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.632-632
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• 2015

기후변화로 정상적인 물 순환 체계가 변화되면서, 건천화로 하천의 정상정인 기능이 어렵게 될 것이다. 농업용 저수지의 둑 높이기 사업으로 수자원의 저류공간이 증가되었고, 확보된 수자원을 기후변화에 대응해 하천유지유량으로 활용 할 수 있게 되었다. 본 연구에서는 기후변화 시나리오 RCP 8.5를 기반으로 임고 저수지의 둑 높이기 사업 전과 후의 공급 가능한 하천유지유량을 평가하였다. 하천유지유량의 공급은 일별 일정량을 공급하도록 하여 이수안전도 90%를 만족하도록 설정하였고, 상시공급과 갈수시 공급으로 구분하여 분석한 결과는 다음과 같다. 첫째, 둑 높이기 사업 전에 하천유지유량의 공급 없이 용수공급 능력을 분석한 결과, 2025s기간, 2055s기간, 2085s기간의 이수안전도는 각각 100%, 93.3%, 96.7%로 분석되어 농업용수의 공급 능력은 충분한 것으로 분석되었다. 둘째, 미래 2025s기간, 2055s기간, 2085s기간에 대하여 둑 높이기 사업 전, 농업용수 공급과 함께 하천유지유량을 상시 공급하였을 경우에는 각각 일별로 3.7천$m^3$, 1천$m^3$, 1.2천$m^3$ 공급 가능하였고, 갈수시만 공급 하였을 경우에는 각각 일별로 7천$m^3$, 2.2천$m^3$, 3.5천$m^3$ 공급 가능한 것으로 분석되었다. 셋째, 미래 2025s기간, 2055s기간, 2085s기간에 대하여 둑 높이기 사업 후, 농업용수 공급과 함께 하천유지유량을 상시 공급하였을 경우에는 각각 일별로 10.5천$m^3$, 4.5천$m^3$, 7천$m^3$ 공급 가능하였고, 갈수시만 공급 하였을 경우에는 각각 일별로 16.6천$m^3$, 7천$m^3$, 12.2천$m^3$ 공급 가능한 것으로 분석되었다. 결론하여 농업용수 공급능력이 충분한 임고 저수지는 둑높이기 사업 후 확보된 수자원을 하천 유지유량으로 공급하면, 증고사업 전보다 상시공급일 경우에 2.8-5.8배, 갈수시 공급일 경우에 2.4-3.4배 많은 양을 공급 할 수 있는 것으로 나타났다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.5
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• pp.403-412
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• 1990

Soybean grain, the main source of protein and lipid, has been used for various purposes in our food resources. Therefore, to abtain and supply protein in safety, soybean must be considered as more and more important and it must be made many efforts in breeding for good quality and high protein. Contents of seed protein of the recommeded soybean cultivars in Korea were above 40 persents. Soybean seed protein was divided four fractions by solubility differences. Contents of fraction-albumines, globulines, prolamines and glutelines- were 7.59%, 71.43%, 1.32%, and 9.11% to total protein in Jangyeobkong. The major components of amino acids were glutamic acid, aspartic acid and arginine in order, cystein, methionine, and tyrosine were the minor components in soybean seed protein. Soybean seed protein is constituted mostly of 11s and 7s proteins. Contents of methionine and cystein, sulfur containing amino acid, in 11s protein has already identified higher than 7s protein's The yields of soybean curd were positively correlated with the soluble protein contents of the soybean varieties.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.314-321
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• 2015

Agar-hydrolyzing bacteria were isolated from the coastal sea water of Jeju Island. One isolate, designated as S4, was selected for further study. The S4 cells were Gram-negative and rod-shaped with smooth beige surfaces and single polar flagellum. Cells were grown at $15-42^{\circ}C$, 0.5-5% (w/v) NaCl, between pH 6.0 and 9.0, and in media containing 0.5-5% (w/v) NaCl. The G+C content was 49.93 mol%. The major fatty acids (>15%) were $C_{18:1}{\omega}7c$, $C_{16:0}$ and Summed feature 3 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}$ 2-OH). Based on 16S rRNA sequencing and biochemical and chemotaxonomic characteristics, the strain was designated as Vibrio sp. S4. In liquid culture supplemented with 0.1% agar the cell density and agarase activity reached a maximum level in 72 h, while agarase activity in the culture without agar was negligible, implying agarose expression is induced by agar. The optimum pH and temperature for the extracellular crude agarase of S4 were 7.0 and $45^{\circ}C$, respectively. However, it also exhibited 98.6% and 87.6% at $40^{\circ}C$ and $50^{\circ}C$, respectively, of the maximum activity seen at $45^{\circ}C$. The crude agarase hydrolyzed agarose into (neo)agarotetraose and (neo)agarohexaose.

• Journal of Korean Neurosurgical Society
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• v.63 no.6
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• pp.707-716
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• 2020

Objective : The function of B7H3, a member of the B7 family of proteins, in neuroblastoma (NB) remains poorly characterized. Here we examine the expression pattern of B7H3 in clinical NB specimens and characterize the phenotype of B7H3 knock-down in NB cell line. Methods : Immunohistochemical (IHC) staining was carried out to assess the expression of B7H3 in clinical NB specimens. Survival association was analyzed using five Gene Expression Omnibus (GEO) datasets (GSE85047, GSE45480, GSE62564, GSE16476, GSE49710). Clonogenic survival and flow cytometry were performed after B7H3 knockdown to assess the cellular proliferation and cell survival in vitro. Impact of B7H3 silencing on NB growth was examined in vivo using the SH-SY5Y xenograft model. Results : On IHC staining, B7H3 was widely expressed in clinical NB specimens. Analysis of the transcriptional profiles of five GEO datasets clinically annotated NB specimens revealed that decreased B7H3 expression was associated with improved overall survival. B7H3 knockdown suppressed the proliferation of the SH-SY5Y NB model in vitro and in vivo. Cell cycle analysis revealed that B7H3 silencing induced G1/S arrest. This arrest was associated with the suppression of E2F1 expression and induction of Rb expression. Conclusion : Our results demonstrate that B7H3 expression correlate with clinical survival in NB patients. Preliminary studies suggest that B7H3 may mediate the G1/S transition.

• Archives of Pharmacal Research
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• v.23 no.1
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• pp.50-53
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• 2000

Diarylheptanoids, (5S)-1,7-bis-(3,4-dihydroxyphenyl)-5-hydroxyheptane-3-one (1, hirsutanonol), $(5S)-1,7-bis-(3,4-dihydroxyphenyl)-heptane-3-one-5-O-{\beta}-D-xylopyranoside (2, oregonin)$, $(5R)-1,7-bis-(3,4-dihydroxyphenyl)-heptane-5-O-{\beta}-D-xylopyranoside (3)$, and $(5R)-1,7-bis-(3,4-dihydroxyphenyl)-heptane-5-O-{\beta}$-D-glucopyranoisde$ (4) were isolated from the leaves of Alnus hirsuta Trucz. The structures of these compounds were identified based on the spectral and physicochemical data.

• Natural Product Sciences
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• v.11 no.3
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• pp.170-173
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• 2005

Compounds of (+)-5,5'-dimethoxylariciresinol (1) and (3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one (2) were isolated from an EtOAc extract of the whole plant of Sasa borealis (Hack.) Makino (Gramineae) for the first time in the present investigation. The structures of compounds were identified by analysis of spectral data including 1D- and 2D-NMR spectra as well as by comparison of their data with the published values. These compounds have never been isolated previously from the family Gramineae.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.1131-1132
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• 2008

In this paper, we developed UHF RFID R/W system using AT91SAM7S256(ARM chip), UHF RFID R/W module (WJ7090) and wireless LAN(IEEE 802.11.a/b). And we developed a transmission/receiving packet which is send to UHF R/W module in AT91SAM7S256. In order to show the usefulness of UHF RFID R/W system, we executed a performance test. The developed UHF RFID R/W system shows better performance for reading of RFID tag and data transmission through wireless LAN.

• Natural Product Sciences
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• v.20 no.2
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• pp.86-90
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• 2014

Phytochemical investigation of the 80% MeOH extract from the leaves of Hosta longipes resulted in the isolation of sixteen compounds (1 - 16). The structures of the compounds were elucidated by spectroscopic methods to be methyl 10,10-dimethoxydecanoate (1), methyl 10-hydroxy-8E,12Z-octadecadienoate (2), methyl coriolate (3), trans-phytol (4), phytene-1,2-diol (5), phyton (6), (3S,5R,6S,7E,9R)-7-megastigmene-3,6,9-triol (7), (3S,5R,6S,9R)-3,6,9-trihydroxymegastigman-7-ene (8), shikimic acid (9), p-coumaramide (10), trans-N-p-coumaroyltyramine (11), cis-N-coumaroyltyramine (12), tryptophan (13), thymidine (14), adenosine (15), and deoxyadenosine (16). Compound 1 was synthesized, but not yet isolated from natural source, and compounds 2-16 were isolated for the first time from this plant source.