• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.64-64
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• 2020

우리나라의 고사리 재배면적은 2018년 기준 5,406ha 수준을 보이고 있으며 전체 산채류 재배면적에서 가장 높은 비중을 차지하고 있다. 고사리(Ferns)는 고사리 속(Pteridium spp.)에 속하는 양치식물의 총칭으로서 우리나라에는 22과 70속 272종이 분포되어 있는 것으로 보고되었다. 고사리의 어린 순에는 가식부 100g 당 칼슘 15.0mg, 칼륨 185.0mg 등이 함유되어 있으며 골다공증, 심혈관 질환 등에 효과가 있고 식이섬유로 인해 변비 예방에도 유용한 것으로 알려져 있다. 고사리 뿌리에서 추출한 전분은 중국 및 일본 등지에서 면류나 제과용으로 이용되고 있어 고사리 재배의 부가가치를 향상시킬 수 있는 방안으로 기대되고 있다. 본 연구에서는 고사리의 재배년차간에 뿌리 전분의 생산성과 이화학적 특성을 조사하기 위해 2020년 3월 26일 각 재배년차별로 굴취한 뿌리를 세척 후 고무망치로 파쇄하고 수침 방법을 사용해 추출한 전분의 이화학적 특성을 조사하였다. 재배년차간 고사리 뿌리의 생산성은 5년생과 7년생에서 3,000 kg/10a 이상으로 유의하게 높은 수량을 보였다. 세척한 고사리 뿌리를 24시간 sodiun metasulfite 4% V/W 용액에 수침하여 전분을 추출한 후 24시간 간격으로 3회에 걸쳐 진탕, 침전과 세척의 과정을 거친 후 조사한 전분 추출 수율은 뿌리 생체중 대비 6.8~7.6% 수준이었으며 재배년차간 차이는 없었다. 추출한 전분의 색도분석에서 L 값은 재배년차가 높아질수록 낮아지는 경향을 보였으나 a 값은 3.7~4.8, b 값은 13.5~15.9로서 뚜렷한 차이를 보이지 않았다. 고사리 뿌리전분의 호화특성에서 최고점도는 3년생 고사리 뿌리에서 추출한 전분이 557.4 RVU로 높은 값을 보였으나 재배년차간 통계적 유의성을 인정할 수는 없었으며, 최저검도에서는 5년생에서 추출한 전분이 269.9RVUfh 낮은 경향을 보였다. 또한 최대 점도 시간은 5.0~5.2hr로 재배년차간 차이는 없었으며 호화 저지온도는 67.0~68.9℃ 범위로 유의한 차이를 보이지 않았다. 고사리 뿌리에서 채취한 전분의 무기물 함량을 조사한 결과 황의 함량은 1년생에서 0.081%로 유의하게 높았으며, 인산 함량은 3년생에서 72mg/kg으로 가장 높았고 1년생에서 31mg/kg으로 가장 낮은 값을 나타냈다. 칼륨의 함량은 7년생에서 추출한 전분에서 376.3mg/kg으로 높았으나 재배년차 간 뚜렷한 경향을 보이지 않았으며 칼슘, 마그네슙 및 철분 함량에서는 5년생 전분에서 유의하게 높은 경향을 보였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.85-85
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• 2020

껍질째 먹는 청포도 품종인 '샤인머스켓'은 망고포도라고 불릴 정도로 단맛이 강하고 풍부한 머스켓향을 가지는 것이 특징이다. 또한 포도알이 크고 무핵처리에 의해 씨가 제거되어 소비자 만족도가 높아 최근 각광받고 있는 포도 품종이다. 샤인머스켓에 대한 국내연구는 주로 재배조건에 따른 과실특성 위주로 보고되고 있어, 본 연구는 소비가 늘고 있는 샤인머스켓의 기능성에 대한 기초자료를 제시하고자 부위별 추출조건에 따른 총폴리페놀, 총플라보노이드 함량 및 항산화 활성을 조사하였다. 샤인머스켓은 2019년 11월에 경북 상주지역에서 생산된 것으로, 과피와 과육의 2가지로 나누어 동결건조하였으며, 추출용매(증류수, 에탄올, 메탄올)와 추출용매농도(30%, 50%, 70%, 100%) 조건에 따라 시료 무게대비 10배의 용매를 가한 후 초음파방법으로 2시간 추출하였으며, 이를 2회 반복하였다. 부위별 총폴리페놀과 총플라보노이드 함량은 과육보다 과피에서 유의적으로 높게 나타났으며, 항산화 활성 또한 과피에서 월등히 높은 값을 나타냈다. 추출조건에 따른 생리활성 물질의 함량과 항산화 활성은 과육과 과피에서 다르게 나타났는데, 과육의 경우 메탄올 추출 시 활성이 높게 나타난 반면, 과피는 에탄올 추출 조건에서 활성이 더 높게 나타났다. 과육의 경우, 70% 메탄올 추출 조건에서 가장 높은 활성을 보였으며, 총폴리페놀 0.99 mg CE/g dw, 총플라보노이드 0.31 mg CE/g dw, DPPH 라디칼 소거능 2.94 mM TE/g dw, ABTS 라디칼 소거능 6.82mM TE/g dw, ORAC 11.40 mM TE/g dw, FRAP 4.92 mM Fe(II)/g dw이었다. 이와 달리 과피는 30% 에탄올 추출 조건에서의 활성이 유의적으로 높게 나타났으며, 총폴리페놀 10.23 mg CE/g dw, 총 플라보노이드 5.22 mg CE/g dw, DPPH 라디칼 소거능 44.72 mM TE/g dw, ABTS 라디칼 소거능 123.25 mM TE/g dw, ORAC 208.38 mM TE/g dw, FRAP 96.24 mM Fe(II)/g dw이었다. 이상의 결과를 볼 때, 껍질째 샤인머스켓을 섭취하는 것은 기능성 측면에서 유익하며, 추후 부위를 나누지 않은 샤인머스켓을 대상으로 추출조건 설정 및 기능성 평가에 대한 연구를 진행할 예정이다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.11a
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• pp.72-73
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• 2023

As containerized maritime transport began in earnest, the size of container ships has steadily increased, and recently, the operation of 24,000 TEU-class vessels has become regular. However, concerns about the efficiency and productivity of such mega container ships from a port operational perspective have continued to be raised. The 10th Busan International Port Conference requested an in-depth study on the trends of container ship enlargement by analyzing the order status of ultra-large container ships from major global liners. Generally, the factor that drives the upsizing of ships is the realization of economies of scale that lowers transportation costs per TEU, which leads to a higher level of cost reduction per unit transportation compared to the increase in fuel consumption due to transporting large amounts of cargo with a single ship. However, it is necessary to examine whether this trend of container vessel enlargement is feasible for port operations. To this end, this study compares and analyzes the productivity and efficeiency of different ship sizes to evaluate the effect of ship size on port operations.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.4
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• pp.524-531
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• 2015

Antioxidant activities and in vitro anticancer effects of bamboo salt doenjang on HT-29 human colon cancer cells were studied. 3Y3B-D (three-year fermentation using three-time baked bamboo salt doenjang), 3Y9B-D (three-year fermentation using nine-time baked bamboo salt doenjang), 6Y3B-D (six-year fermentation using three-time baked bamboo salt doenjang), and 6Y9B-D (six-year fermentation using nine-time baked bamboo salt doenjang) were compared to C-D (commercial doenjang) and 3B-S (cooked soy beans prepared using three-time baked bamboo salt). There were no differences between experimental groups in pH, amino-type nitrogen, or ammonia-type nitrogen levels. 6Y9B-D showed the highest antioxidative effect, followed by 6Y3B-D, 3Y9B-D, and 3Y3B-D, in order. 6Y9B-D showed the highest total polyphenol concentration. 6Y9B-D showed the highest anticancer effect, as determined by MTT assay, as well as levels of the pro-inflammatory cytokines including TNF-${\alpha}$, IL-6, iNOS, and COX-2, followed by 6Y3B-D, 3Y9B-D, and 3Y3B-D, in order. From the results above, 6Y9B-D showed the highest antioxidative and anticancer effects, followed by 6Y3B-D, 3Y9B-D, 3Y3B-D, C-D, and 3B-S.

• Clinical and Experimental Pediatrics
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• v.48 no.4
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• pp.395-400
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• 2005

Purpose :Rotavirus is one of the most important causes of nosocomial infections among children. The aim of this study is to determine the risk of nosocomial rotavirus infections and to evaluate the effectiveness of breast-feeding and probiotics in the prevention of nosocomial rotavirus infections. Methods : This study was carried out on admitted children without diarrhea between March 1, 2003 and February 29, 2004. Three hundred ninety patients aged 4 days to 13 years during this study were available. We examined the feces of all children for rotavirus by latex agglutination on admission, during hospital, and after discharge, to see whether they developed diarrhea or not. Results : Nosocomial rotavirus infections was significantly increased with children under 12 months of age(P=0.008). The monthly attack rate was great between December and March(P=0.046). Prolonged hospital stay was associated with an increased attack rate of nosocomial rotavirus infections (P=0.003). The risk of nosocomial rotavirus infections was not associated with the number of roommates and whether or not they were breast-fed or fed on probiotics. Conclusion : Nosocomial rotavirus infections are significantly more likely to occur in children under 12 months of age, admitted between December and March, and with prolonged hospital stays. Prompt identification and isolation of children with nosocomial rotavirus infections, even without diarrhea, may decrease rates of nosocomial rotavirus infections.

• Tuberculosis and Respiratory Diseases
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• v.57 no.4
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• pp.311-319
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• 2004

Background : It has been well known that bronchiectasis (BE) is a predisposing condition for pulmonary NTM infection, whereas there are some suggestions that BE, especially nodular BE, may be a result of NTM pulmonary infection. This retrospective study was done to investigate the prevalence of NTM pulmonary infection in the patients with BE. Methods : Eight hundred sixty-six patients, who underwent chest CT and sputum AFB examination and had BE detected by chest CT at Asan Medical Center in 2002, were included in this study. They were divided into Group I (bilateral BE, especially in RML, lingular or both lower lobes; 134), Group II (BE accompanied with fibrocavitary lesions commonly found in tuberculosis, usually both upper lobes; 233) and Group III (except Group I, II; 499) according to the radiological findings. Group I was subdivided into Group I+ (62) or Group I- (72) according to the presence or absence of centrilobular nodules, respectively. The sputum AFB examination, clinical and radiological findings were analyzed and compared between groups. Results : The number of patients who had at least one positive NTM culture was significantly higher in Group I+ compared with others (p<0.05); 24.2% in Group I+, 6.9% in Group I-, 9.9% in Group II, 6.0% in Group III and 4.1% in control. The number of patients who had true NTM infection defined by ATS guideline was higher in Group I+ (5, 8.1%) compared with others (p<0.05). In all groups, M. avium-intracellulare comlex was the most common isolates. Conclusion : Even though true NTM pulmonary disease was more prevalent in the patients with nodular BE, especially located in RML, left linguar, or both lower lobes, only a small population of the patients with nodular BE met the ATS diagnostic criteria for NTM pulmonary disease. The other patients in nodular BE group may have subclinical stage of NTM infection or completely different diseases from NTM infection. Long-term clinical studies are needed to clarify this issue.

• Journal of Nutrition and Health
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• v.53 no.3
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• pp.288-302
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• 2020

Purpose: This study examined the factors related to skipping breakfast in high school girls. Methods: A survey of a total of 581 high school girls residing in Incheon was conducted. The subjects were classified into 2 groups according to the breakfast frequency: "breakfast skipping" group (eating breakfast ≤ 2 times/week, n = 250) and "breakfast eating" group (eating breakfast ≥ 3 times/week, n = 331). This study compared the general characteristics, lifestyles, interest in weight control and beauty care, food habits, the reasons and symptoms of skipping breakfast, as well as the attitude toward breakfast between the 2 groups. Results: The mean age of the subjects was 16.5 years old. Approximately, 33.9%, 33.6%, and 32.5% of the subjects were in first grade, second grade, and third grade, respectively, with no significant difference between the 2 groups. The breakfast skipping group showed a high interest in weight control, and beauty care than the breakfast eating group. The breakfast skipping group consumed more afternoon snacks and late-night snacks, and ate dinner irregularly. Although the breakfast skipping group experienced the negative effects of skipping breakfast, they showed low recognition for the importance or role of breakfast. The most common reason for skipping breakfast was lack of time, which appears to be related to the late wake-up time and the time spent on make-up or beauty-care. Conclusion: Skipping breakfast by high school girls was related to a high interest in beauty care, irregular meals and low attitude toward the importance of breakfast for health and nutrition. Therefore, it is necessary to conduct nutrition education to change the perceptions and attitudes of female adolescents regarding the importance of breakfast by recognizing that breakfast can affect not only balanced nutrition and health, but also healthy physical beauty.

• Journal of dental hygiene science
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• v.5 no.2
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• pp.63-70
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• 2005

The relation among the variables of dental hygienists' both personal protection and infection prevention education was investigated in order to identify the necessity of realization of personal protection and utilize in the development of educational programs. The study tools included structured questionnaire and in-home interview. Data analysis included frequency and t-test, and the relations among the variables were as below: (1) Dental hygienist's maskwearing was most well observed in Kyonggi-do (90.0%) by locations, and in the dental hospitals (84.8%) by working places, which was statistically significant. (2) Glove-wearing was most well observed in the university and the higher graduates population (59.1%) by academic background, and in the dental hospital (73.9%) by working place, which was statistically significant. (3) Protection glasses-wearing was most well observed in Guns, Ups, and Myons (82.4%) by locations, which was statistically significant. (4) Uniforms were hand-laundered at home in the 40.9% of the university and the higher graduates, which was statistically significant. (5) About infection management, 67.0% of 2-year college graduates and 71.7% of the respondents from dental hospitals had been educated during their college days, which was statistically significant. (6) About the necessity of infection education, 77.3% of the university graduates responded it is very necessary, which was statistically significant.

• Tuberculosis and Respiratory Diseases
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• v.62 no.5
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• pp.406-416
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• 2007

Background: Single nucleotide polymorphisms (SNPs), which consist of a substitution of a single nucleotide pair, are the most abundant form of genetic variations occurring with a frequency of approximately 1 per 1000 base pairs. SNPs by themselves do not cause disease but can predispose humans to disease, modify the extent or severity of the disease or influence the drug response and treatment efficacy. Single nucleotide polymorphisms (SNPs), particularly those within the regulatory regions of the genes often influence the expression levels and can modify the disease. Studies examining the associations between SNP and the disease outcome have provided valuable insight into the disease etiology and potential therapeutic intervention. Traditionally, the genotyping of SNPs has been carried out using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), which is a low throughput technique not amenable for use in large-scale SNP studies. Recently, TaqMan real-time PCR chemistry was adapted for use in allelic discrimination assays. This study validated the accuracy and utility of real-time PCR technology for SNPs genotyping Methods: The SNPs in promoter sequence (-37 and -524) of lung cancer suppressor gene, RRM1 (ribonucleotide reductase M1 subunit) with the genomic DNA samples of 89 subjects were genotyped using both real-time PCR and PCR-RFLP. Results: The discordance rates were 2.2% (2 mismatches) in -37 and 16.3% (15 mismatches) in -524. Auto-direct sequencing of all the mismatched samples(17 cases) were in accord with the genotypes read by real-time PCR. In addition, 138 genomic DNAs were genotyped using real-time PCR in a duplicate manner (two separated assays). Ninety-eight percent of the samples showed concordance between the two assays. Conclusion: Real-time PCR allelic discrimination assays are amenable to high-throughput genotyping and overcome many of the problematic features associated with PCR-RFLP.

• The Korean Journal of Nuclear Medicine Technology
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• v.18 no.2
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• pp.68-72
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• 2014

Purpose The nuclear medicine examination, there is a difficulty to carry out the inspection of both on the day of residual isotope due to the half-life. In this study, by studying the mutual influence and $^{18}F$-FDG of $^{99m}TcO_4{^-}$, I would like to explain the matters to be considered in the case of performing the same day. Materials and Methods With the NEMA-1994 Phantom, and experiments were performed 3 times. Create a 1: 4 Background ratio HOT and the $^{99m}TcO_4{^-}$ The first experiment: After underwent SPECT in INFINIA (GE Healthcare, MI, USA), and were injected with $^{18}F$-FDG 37 MBq in the Background area, 13 once for 60 minutes under the same conditions was time Scan. Create a 1: 4 Background ratio HOT and the $^{18}F$-FDG second is: The Scan in PET/CT Discovery 600 (GE Healthcare, MI, USA), and 148 MBq after injection $^{99m}TcO_4{^-}$ the Background area, once for 60 minutes, 6 under the same conditions was time Scan. Create a 1: 4 Background ratio HOT and the $^{18}F$-FDG experiments las, increments of 296 MBq and 148 MBq the 1 Bed Scan after $^{99m}TcO_4{^-}$, was 1 Bed Scan under the same conditions. Non BKG area and HOT, I was measured comparing the Total Counts and SNR or CNR. Results Showed a significant difference in the ratio CNR of enforcement during SPECT $^{18}F$-FDG is, (p>0.05). The $^{99m}TcO_4{^-}$ was no significant difference between the SNR ratio of PET / CT at the time of the effective date (p<0.05). I got the results $^{99m}TcO_4{^-}$ that reduce the Total Counts of PET / CT scan. Conclusion If you make a PET / CT scan, may affect the test using the $^{99m}TcO_4{^-}$ up to 12 hours, when it is performed before the $^{99m}TcO_4{^-}$, does not affect the SNR and SUV, PET / CT scan I reduced the detection efficiency. The inspection of day, we'd like to recommend a way to complement the detection efficiency to increase the inspection time of PET / CT in move forward the inspection using the $^{99m}TcO_4{^-}$.