• Title/Summary/Keyword: 6q24

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Lmbr1 Expression in Early Embryo Development Stages in White Leghorn and Chinese Silky

  • Zhang, Ying;Xu, Weizhuo;Li, Ning
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.1
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    • pp.7-12
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    • 2009
  • Lmbr1 is regarded as a key gene that controls the digital model formation in early developmental stages of the chicken. However, there are few reports of lmbr1 expression levels and tendencies in 4-toe and 5-toe chicken species. Therefore, the objective of this study was to compare the lmbr1 expression in White Leghorn (4-toe) and Chinese Silky (5-toe). Firstly, total RNA was extracted from 14 different embryonic development stages (HH3 to HH31) in White Leghorn and Chinese Silky. Secondly, dramatic gene expression changes of lmbr1 were monitored by RT-PCR, which indicated a general up-down-up tendency with subtle differences between these two species. Moreover, Q-PCR reactions were performed to quantitate the expression level of lmbr1 in the 14 selected developmental stages. These data demonstrated a first lmbr1 expression peak of 18.68 and 15.32, a lmbr1 expression trough of 6.61 and 1.80, and a second lmbr1 expression peak of 22.33 and 12.48 in White Leghorn and Chinese Silky, respectively. Finally, embryonic in situ hybridization analysis identified that lmbr1 expressed in the ectoderm in HH21, HH23 and HH24 developmental stages in both species.

Effects of Levels of L-Leucine Supplementation with Sub-optimal Protein in the Diet of Grower-finisher Broiler Chickens on Carcass Composition and Sensory Characteristics

  • Erwan, E.;Alimon, A.R.;Sazili, A.Q.;Yaakub, H.;Karim, R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.5
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    • pp.650-654
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    • 2011
  • An experiment involving 180 straight run one-day-Cobb broilers was conducted to evaluate the effect of supplementation of L-leucine with different levels of crude protein (CP) on carcass composition and sensory characteristics of broiler grower-finisher chickens. Six experimental diets comprising two levels of crude protein (CP) i.e., 20 and 20% with three levels of L-leucine i.e. 0, 0.5 and 0.67%, were offered to birds from 21-42 d of age. The birds were randomly divided into 36 experimental pens, 5 chickens in each pen, and there were 6 replicates under each diet. L-leucine supplementation did not affect the bone and lean, whereas fat was decreased (p<0.05) when L-leucine was added at 0.5%. Similarly, there were no significant differences (p>0.05) in the lean, fat and bone among chickens fed two levels of CP. No significant differences between dietary treatments were observed on any sensory characteristics affected by dietary L-leucine and CP. From this study, it is obvious that supplementation of up to 0.5% L-leucine reduced fat. However, other characteristics were not affected by supplementation of L-leucine. Similarly, reduction of body composition and sensory characteristics were not apparent on a diet low in CP.

The Factors related to Dyslipidemia and Hypertension among Male Office Workers (사무직 남성 근로자의 이상지질혈증과 고혈압 영향 요인)

  • Lee, Eun Kyung;Kim, Ok Soo
    • Korean Journal of Adult Nursing
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    • v.25 no.4
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    • pp.432-443
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    • 2013
  • Purpose: The purpose of this study was to investigate the factors related to dyslipdemia and hypertension among male office workers. Methods: This study was a cross-sectional survey. The sample was 204 male office workers aged 30 to 62 years without cardiovascular disease and not taking medication for hypertension and dyslipidemia. Data were collected from November, 2011 to March, 2012. Dyslipidemia was examined by checking serum lipid profiles. Results: Dyslipidemia was related to career (adjusted OR 1.06, 95% CI 1.03~1.51), time spent at desk (adjusted OR 1.25, 95% CI 1.03~1.51), job stress of Q25-49 (adjusted OR 2.72, 95% CI 1.24~5.93), inactivity (adjusted OR 6.86, 95% CI 2.62~17.93), and snack intake frequency (adjusted OR 1.57, 95% CI 1.03~2.38). Hypertension was related to career (adjusted OR 1.07, 95% CI 1.01~1.14), heavy drinking (adjusted OR 5.00, 95% CI 1.25~20.04), and snack intake frequency (adjusted OR 2.10, 95% CI 1.33~3.34). Conclusion: Work and lifestyle-related factors were associated with dyslipidemia and hypertension in male office workers. These findings suggest that lifestyle intervention and improvement of working conditions are integral parts of cardiovascular disease prevention.

Fluctuations of Coastal Water Temperatures Along Korean and Japanese Coasts in the East Sea

  • KANG Yong-Q.;CHOI Seong-Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.6
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    • pp.351-360
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    • 1988
  • Based on historic data of monthly means of sea surface temperatures (SST) for 24 years $(1921\~1944) $ at 23 Korean and Japanese coastal stations in the East Sea (the Japan Sea), we analyzed spatio-temporal characteristics of coastal SST and SST anomalies. The means of SST at Korean coast are higher than those at Japanese coast of the same latitudes, and the annual range of SST at Korean coast are larger than those at Japanese coast. Empirical orthogonal function analysis shows that almost all $(96\%)$ of the SST fluctuations are described by simultaneous seasonal variations. The flurtuations of SST anomalies are small in the Korea Strait and large at the boundaries between the warm and told currents in the basin. The fluctuations of SST anomalies along Korean coast are correlated each other The same is true for SST anomalies along Japanese coast. However, there is only weak correlation between the SST anomalies at Korean coast and those at Japanese coast. Empirical orthogonal function analysis shows that $27\%$ of the coastal SST anomalies in the East Sea are described by simultaneous fluctuations, and $12\%$ of them are described by alternating fluctuations between Korean and Japanese coasts.

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Implementation and Design of Digital Instruments System using FPGA (FPGA를 이용한 디지털 계측 시스템의 설계 및 구현)

  • Choi, Hyun Jun;Jang, Seok Woo
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.2
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    • pp.55-61
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    • 2013
  • A field-programmable gate array (FPGA) is an integrated circuit designed to be configured by a customer or a designer after manufacturing. The FPGA configuration is generally specified using a hardware description language (HDL), similar to that used for an application-specific integrated circuit (ASIC) (circuit diagrams were previously used to specify the configuration, as they were for ASICs, but this is increasingly rare). Contemporary FPGAs have large resources of logic gates and RAM blocks to implement complex digital computations. In this paper, we implement a system of digital instrumentation using FPGA. This system consists of the trigger part, memory address controller part, control FSM part, Encoder part, LCD controller part. The hardware implement using FPGA and the verification of the operation is done in a PC simulation. The proposed hardware was mapped into Cyclone III EP2C5Q208 from Altera and used 1,700(40%) of Logic Element (LE). The implemented circuit used 24,576-bit memory element with 6-bit input signal. The result from implementing in hardware (FPGA) could operate stably in 140MHz.

A Content Analysis of Research on Infant/Child-Teacher Attachment in Korea : 1993-2010 (영유아-교사 애착에 관한 국내 연구 동향 : 1993년-2010년)

  • Chae, Jin-Young
    • The Korean Journal of Community Living Science
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    • v.21 no.4
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    • pp.517-528
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    • 2010
  • The number of young children who attend daycare center, preschool or kindergarten and are taken care of by teachers during a day has skyrocketed in Korea. The purpose of this study was to analyze the contents of the research on infant/child-teacher attachment in Korea. Thirty five studies (5 doctoral dissertations, 24 masters' theses, and 6 academic journal articles) published from 1993 to 2010 were analyzed in the matter of the publication period, the related variables, and methodology for analyzing the data. The findings are as follows: 1) More than two-thirds of the research were from masters' theses. The number of studies conducted in the late 2000s doubled compared to that in the 1990s. 2) Each study on infant/child-teacher attachment had at least one related variable regarding social, emotional, cognitive or physical development, but not language. The studies on infant/child-teacher attachment related to social development have rapidly increased in the late 2000s. 3) The majority of studies were conducted using quantitative analyses and Attachment Q-set version 3.0. Difference analysis and relational analysis were most frequently used in many studies. There was only one study which was analysed with the advanced statistical methodology. The implication for a future study was also discussed.

Genome-Wide Association Study Identifies Candidate Loci Associated with Platelet Count in Koreans

  • Oh, Ji Hee;Kim, Yun Kyoung;Moon, Sanghoon;Kim, Young Jin;Kim, Bong-Jo
    • Genomics & Informatics
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    • v.12 no.4
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    • pp.225-230
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    • 2014
  • Platelets are derived from the fragments that are formed from the cytoplasm of bone marrow megakaryocytes-small irregularly shaped anuclear cells. Platelets respond to vascular damage, contracts blood vessels, and attaches to the damaged region, thereby stopping bleeding, together with the action of blood coagulation factors. Platelet activation is known to affect genes associated with vascular risk factors, as well as with arteriosclerosis and myocardial infarction. Here, we performed a genome-wide association study with 352,228 single-nucleotide polymorphisms typed in 8,842 subjects of the Korea Association Resource (KARE) project and replicated the results in 7,861 subjects from an independent population. We identified genetic associations between platelet count and common variants nearby chromosome 4p16.1 ($p=1.46{\times}10^{10}$, in the KIAA0232 gene), 6p21 ($p=1.36{\times}10^{-7}$, in the BAK1 gene), and 12q24.12 ($p=1.11{\times}10^{-15}$, in the SH2B3 gene). Our results illustrate the value of large-scale discovery and a focus for several novel research avenues.

Creep Characterization of Type 316LN and HT-9 Stainless Steels by the K-R Creep Damage Model

  • Kim, U-Gon;Kim, Seong-Ho;Ryu, U-Seok
    • Journal of Mechanical Science and Technology
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    • v.15 no.11
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    • pp.1463-1471
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    • 2001
  • The Kachanov and Rabotnov (K-R) creep damage model was interpreted and applied to type 316LN and HT-9 stainless steels. Seven creep constants of the model, A, B, $textsc{k}$, m, λ, ${\gamma}$, and q were determine d for type 316LN stainless steel. In order to quantify a damage parameter, the cavity was interruptedly traced during creep for measuring cavity area to be reflected into the damage equation. For type 316LN stainless steel, λ= $\varepsilon$R/$\varepsilon$* and λf=$\varepsilon$/$\varepsilon$R were 3.1 and increased with creep strain. The creep curve with λ=3.1 depleted well the experimental data to the full lifetime and its damage curve showed a good agreement when r=24. However for the HT-9 stainless steel, the values of λ and λf were different as λ=6.2 and λf=8.5, and their K-R creep curves did not agree with the experimental data. This mismatch in the HT-9 steel was due to the ductile fracture by softening of materials rather than the brittle fracture by cavity growth. The differences of the values in the above steels were attributed to creep ductilities at the secondary and the tertiary creep stages.

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Purification and Enzymatic Characteristics of Myrosinase from Korea Cabbage (배추 Myrosinase의 정제 및 효소학적 특성)

  • 심기환;강갑석;서권일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.4
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    • pp.563-569
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    • 1995
  • Myrosinase from Korean cabbage(Bogdoli) was purified and its enzymatic properties were investigated. Myrosinase from the Korean cabbage was purified by DEAE Bio-Gel Sepharose, Concanavalin-A, and Mono-Q column chromatography and exhibited a 55KD molecular weight with a single band on the gel of SDS-PAGE. The enzyme was purified about 21-fold compared to its crude enzyme and a specific activity of purified enzyme was 15, 120units/mg. Optimum pH of the myrosinase was 7.0 in both phosphate and Tris-HCl buffer solutions, the enzyme was stable at pH 6.5~7.0. Optimum temperature of enzyme was 37~38$^{\circ}C$. The enzyme activity was significantly inhibited by Cu2+ and Hg2+, but enhanced by ascorbic acid, resulting in a maximum activity at 1mM ascorbic acid. Among the ascorbic acid analogues, dehydro-ascorbic acid did not affect, whereas others showed a little effect on the enzyme activity, but less than ascorbic acid itself. Reducing agents such as 2-mercaptoethanol and dithiothreitol had no effect on the enzyme activity, but the enzyme activity was enhanced when 2-mercaptoethanol was mixed with ascorbic acid.

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New Approaches to Xerostomia with Salivary Flow Rate Based on Machine Learning Algorithm

  • Yeon-Hee Lee;Q-Schick Auh;Hee-Kyung Park
    • Journal of Korean Dental Science
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    • v.16 no.1
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    • pp.47-62
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    • 2023
  • Purpose: We aimed to investigate the objective cutoff values of unstimulated flow rates (UFR) and stimulated salivary flow rates (SFR) in patients with xerostomia and to present an optimal machine learning model with a classification and regression tree (CART) for all ages. Materials and Methods: A total of 829 patients with oral diseases were enrolled (591 females; mean age, 59.29±16.40 years; 8~95 years old), 199 patients with xerostomia and 630 patients without xerostomia. Salivary and clinical characteristics were collected and analyzed. Result: Patients with xerostomia had significantly lower levels of UFR (0.29±0.22 vs. 0.41±0.24 ml/min) and SFR (1.12±0.55 vs. 1.39±0.94 ml/min) (P<0.001), respectively, compared to those with non-xerostomia. The presence of xerostomia had a significantly negative correlation with UFR (r=-0.603, P=0.002) and SFR (r=-0.301, P=0.017). In the diagnosis of xerostomia based on the CART algorithm, the presence of stomatitis, candidiasis, halitosis, psychiatric disorder, and hyperlipidemia were significant predictors for xerostomia, and the cutoff ranges for xerostomia for UFR and SFR were 0.03~0.18 ml/min and 0.85~1.6 ml/min, respectively. Conclusion: Xerostomia was correlated with decreases in UFR and SFR, and their cutoff values varied depending on the patient's underlying oral and systemic conditions.