• The Plant Pathology Journal
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• v.17 no.3
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• pp.164-168
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• 2001

Rice black-streaked dwarf virus (RBSDV) and Maize rough dwarf virus (MRDV) are closely related viruses. Since the two viruses produce identical symptoms on maize, barley, and wheat, diagnosis of infected plants is difficult. Previously, we reported that partial cDNA clones of RBSDV S5 and S6 from the Japanese isolate (RBSDV-H) have lower sequence homology to MRDV than do cDNA clones from other genomic segments. In order to test whether cDNA clones of RBSDV-H S5 and S6 can be used for molecular diagnosis, RBSDV field isolates from Korea and from Hokkaido, Japan were tested in dot blot hybridizations probed with RBSDV-H S5 and S6 cDNA colnes. Hybridization with these probes was more intense against the RBSDV genome than against the MRDV genome. Therefore, RBSDV-H S5 and S6 cDNA clones can be used to differentiate between the two viruses. Furthermore, RBSDV-H S5 and S6 clones reacted more strongly against the viruses from stunted maize plants from Korean fields than to MRDV, indicating that RBSDV may be the causal disease agent in maize plants in Korea.

• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.259-266
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• 1987

A total of 129 clinical isolates of Staphylococcus species was characterized by the tests of coagulase production, haemagglutination, mannitol fermentation, DNase production and hemolysis. Ninety-nine out of them showed positive reactions to the tests, therefore they were identified as Staphylococcus aureus. The isolates showing positive reaction in haemagglutination test also showed 100% of tube coagulase positive reaction. The haemagglutination test was a reliable method for identifying Staphylococcus aureus in the clinical laboratory. S. aureus produced stronger hemolysis with human blood agar than with sheep blood agar. Antibiotic resistant S. aureus isolates(S-46, S-112, S-126) had 4 to 6 p]asmid DNA elements. The S-112 strain had 6 plasmid DNA elements(1.8, 2.2, 3.7, $26.3{\sim}50$, and 70 Mdaltons), the S-126 had 4 elements(2.6, 4.2, $4.6{\sim}60Md$), and the S-46 had 1 element(${\sim}100Md$). PPSA strain had 4 plasmid DNA elements(2.5, 4.2, $4.6{\sim}60Md$) and S. aureurs(ATCC) strain contained 9.4, 26.3 and ${\sim}50Md$ plasmid DNA elements.

• Journal of the East Asian Society of Dietary Life
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• v.13 no.6
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• pp.586-592
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• 2003

This research used High Performance Liquid Chromatography(HPLC) to analyze and compare the content and the pattern of chlorophyll to find the difference in quality, water temperature, and the stirring time between the three different types of Korean and Japanese powder green tea, which were currently on the market. Chlorophyll contents of Japanese-made medium quality tea (S5: 8.34mg/g), and low quality tea (S6: 8.23mg/g) were relatively high, and that of Korean-made medium quality tea (S2 ; 4.87mg/g) was lower. The water temperature of 85$^{\circ}C$ and 95$^{\circ}C$ were high except in Korean-made medium and low quality powder green tea (S2 and S3). Japanese-made powder tea required 60 seconds of stirring time and Korean-made tea required 15 seconds, but 30 seconds seem to be the ideal stirring time for both of them. Chlorophyll a, b, pheophytin a, b, and pheophorbide were confirmed from the chlorophyll pattern. There were 1.5∼2 times more of chlorophyll a in Japanese-made one compared to that of Korean-made one; also 1.5∼2 times more of pheophytin a in Korean-made one than Japanese-made one. It might be concluded that Japanese-made powder green tea was higher in quality.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.6
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• pp.180-189
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• 2012

This study was carried out to suggest an experiment based for selecting Sedum, which can adapt well with heat tolerance in extensive green roof system. The heat tolerance of Sedum subject to laboratory high temperature treatment and heat processing time were evaluated using electrolyte leakage, chlorophyll content and regrowth test, and the relation between soil water content and heat tolerance were researched. Logistic model of nonlinear regression analysis was used to evaluate the lethal temperatures that were predicted with the range of $45.0{\sim}48.1^{\circ}C$(soil water content 5%), $47.5{\sim}49.3^{\circ}C$(10%), $48.6{\sim}52.8^{\circ}C$(15%) in 6-hours high-temperature treatment. The higher the soil water content, the stronger the heat resistance property of Sedum. there is. The higher the treatment temperature, the lower the chlorophyll content, and the less the soil water content, the faster the chlorophyll decomposition. The order of hot-temperature resistance was S. reflexum>S. takevimense>S. middendorffianum>S. album>S. sieboldii>S. spurium when soil water content was 5%. The order of hot-temperature resistance was S. album>S. reflexum>S. spurium>S. takevimense>S. middendorffianum>S. sieboldii when soil water content was 15%. The more of soil water content, S. album, S. reflexum, S. spurium had stronger tolerant of hot temperature. These results were consistent with those from the regrowth test and the heat tolerance tested by electrolyte leakage evaluation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.5
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• pp.523-526
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• 2012

This study evaluated the effects of the number of feeding days and feeding rate on the growth of growing-out flounder Paralichthys olivaceus. Three replicated groups of fish ($141{\pm}1.9g$) were hand-fed a commercial feed under different feeding regimes for 9 weeks. Fish in group 6F-1S were fed to satiation twice daily for 6 consecutive days and starved for 1 day. Fish in groups 4F-1S and 3F-1S were fed to satiation twice daily for 4 and 3 days, respectively, and starved for 1 day. Fish in group 6F-90% were fed a diet of 90% of 6F-1S twice daily for 6 consecutive days and starved for 1 day. The weight gain, feed efficiency and daily feed intake of the fish groups in 4F-1S and 3F-1S were not significantly different from those of group 6F-1S. The weight gain and feed efficiency of fish in group 6F-90% were not significantly different from those of group 6F-1S. The results of this study suggest that the growth of growing-out flounder fed to satiation twice daily for 4 or 3 days after starving 1 day was similar to that of fish fed to satiation for 6 days, and that the proper feeding rate for growth could be lowered to 90% of satiation without growth suppression.

• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.210-212
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• 1987

한국산 모기에 대한 조사연구사업의 일환으로 1387년 6월 경기도 남양주군 광릉에 위치한 임업시험장 일대에서 야외채집을 실시한 결과 미기록종 Aedes bekkui(베꾸숲모기)를 채집하였기에 이를 보고한다. 성충의 특징은 기문하구(subspiracular area)에 작을 강모가 없는 점이고, 유충의 특징은 머리털 4-C와 6-C의 간격이 좁고 5,6-C의 크기와 모양은 서로 대등하며 6,7-C의 간격은 5,6-C의 간격보다 2배이상 넓다는 점이다. 또 꼬리틸 3-X는 단모로 되어 있고 2-X보다 상당히 길다. Tanaka의 검색표에 따르면 Aedes bekkui 성충의 특징은 아속 Edwardsaedes와 일치되지만 유충은 아속 Aedimorphus에 일치된다. 이는 Belkin이 처음 아속 Edwardsaedes를 확립할 때 유일하게 Aedes imprimens를 단모식 표본종(monotypic species)으로 이용하여 그 특징 열거에 있어 너무 단조로 왔기 때문이며 또한 일본에서 A. imprimens가 A. bekkui로 판명되기까지 자충의 특징만이 기록되어 왔기 때문이다. 그러므로 과거 한국에서 보고된 A. imprimens도 일본의 경우와 같이 A. bekkui의 오식종이었을 것으로 생각된다.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1331-1335
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• 2000

The WL6 strain isolated from Kimchi could not be made scientific name because it was identified as three species, i.e., Leuconostoc mesenternides ssp cremoris, Leu. mesenteroides ssp. dextranicum or Lactobacillus bifermentans when it was tested by API kit or Biolog system methods. The unidentifiable WL6 strain was finally reclassified as Lactobacillus bifermentans by genetic identification using two PCR-based specific sequence primer sets which were originated from homologous pepN and 16S rRNA genes.

• Molecules and Cells
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• v.24 no.3
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• pp.316-322
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• 2007

Glutaredoxins (Grxs), also known as thioltransferases (TTases), are thiol oxidoreductases that regulate cellular redox state in a variety of organisms. In the budding yeast Saccharomyces cerevisiae, Grx1 and 2 are cytosolic dithiol Grxs, while Grx3, 4 and 5 are monothiol Grxs. A gene encoding a new monothiol Grx, Grx6, was cloned from the genomic DNA of S. cerevisiae by PCR. Its DNA sequence contains 1,080 bp, and encodes a putative protein of 203 amino acid residues containing Cys-Phe-Tyr-Ser at the active site. Grx6 is similar to other monothiol Grxs in the same organism and to Grx3 in the fission yeast Schizosaccharomyces pombe. and its predicted three-dimensional structure resembles that of S. pombe Grx3. S. pombe cells harboring plasmid pFGRX6 containing the Grx6 gene had about 1.3-fold elevated Grx activity in the exponential phase, and grew better than the control cells under some stressful conditions. Synthesis of ${\beta}$-galactosidase from a Grx6-lacZ fusion gene in S. pombe was enhanced by potassium chloride, aluminum chloride and heat ($37^{\circ}C$) treatment. S. pombe cells harboring plasmid pFGRX6 had elevated ROS levels whereas S. pombe cells harboring extra copies of Grx3 had reduced ROS levels.

• Journal of dental hygiene science
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• v.12 no.4
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• pp.310-319
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• 2012

In this study, 1,495 cases of computerized data collected as disease entities of oral and maxillofacial injuries among patient visiting S university hospital located in Seoul in 2009 were analyzed and following results were obtained. It was found that the injury incidence in male was greater than in female (1.49:1), it occurred most frequently in the age of 7~14 years old (19.6%), and its most frequent cause was falling down (25.9%). Injuries occurred in other place including beach and open-air (24.7%) most frequently, often developed in anterior teeth (43.2%) and posterior teeth (43.1%), their incidence was relatively higher between 15:00~17:00 (10.4%) and 19:00~23:00 (7.1%) O'clock. In the relationship between causes of the injury and the diagnosis based on the international classification of disease), falling, fall, impingement, violence, traffic accident, sports and own making accident caused most frequently lip and oral open injuries (S01.5), open fracture of tooth (S02.51), close fracture of tooth (S02.50), close fracture of mandible (S02.60), close fracture of tooth (S02.50), respectively. From the above results, it is necessary to understand general characteristics of oral and maxillofacial injuries and to consider their trends for the establishment of systematic complement policies and for the performing safety training and public relations activities.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.192-198
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• 2012

Experiments were conducted to investigate the optimum concentration of nutrient solution in hydroponics for strawberry 'Ssanta' bred at Gyongsangbuk-do Agricultural Research & Extension Services. Nutrient solutions for strawberry, which made by Yamazaki, were supplied EC (Electrical Conductivity) 0.6, 0.8, 1.2, and $1.8dS{\cdot}m^{-1}$ after planting on cocopeat medium during experiment period. Growth of shoot of strawberries did not show statistical differences among treatments. Fruit length showed the longest in EC $0.8dS{\cdot}m^{-1}$ in all clusters. In the second flower cluster, fruit length showed longer in EC 0.8 and $1.2dS{\cdot}m^{-1}$ than EC 0.6 and $1.8dS{\cdot}m^{-1}$. In the third flower cluster, it showed the longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$, followed by 0.6 and $1.8dS{\cdot}m^{-1}$. The longest was in EC $0.8dS{\cdot}m^{-1}$ and the shortest in EC $1.8dS{\cdot}m^{-1}$ in the fourth flower cluster. Fruit diameter did not show significant differences among treatments, but longest in EC 0.8 and $1.2dS{\cdot}m^{-1}$ in all clusters. The heaviest mean fruit weight appeared in EC $0.8dS{\cdot}m^{-1}$ in all flower clusters. And heavier in EC $1.2dS{\cdot}m^{-1}$ in the second and third clusters. Also the weight was significantly light in plants grown in EC 0.6 and $1.8dS{\cdot}m^{-1}$ in the second and third cluster. Soluble solids of fruit was the highest in EC $0.6dS{\cdot}m^{-1}$ in all clusters. As the results, we came to the conclusion that the optimum EC for strawberry 'Ssanta' was EC $0.8{\sim}1.2dS{\cdot}m^{-1}$ in this experiment.