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Sorbic Acid Contents Survey on Ham, Sausage and Dried Meat in Market (시판 햄류, 소시지류, 건조저장육류 중 소르빈산 함량조사)

  • 함희진;양윤모;윤은선
    • Journal of Food Hygiene and Safety
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    • v.18 no.1
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    • pp.30-32
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    • 2003
  • This study was performed to investigate the contents of nitrites in 450 meat products in Seoul from Jan. to Dec. in 2002. Sorbic acid contents of the samples were determined by Gas Chromatography. 67 samples of 450 (14.9%) were detected in sorbic acid contents. In each meat products, 65,6% (21/32) in dried meats, 17.6% (3/17) in bacon, 11.7% (27/231) in hams, 11.7% (13/111) in sausages, and 11.5% (3/26) in meat cu. The concentration range of Sorbic acid and its average content in each of the meat products are as follows: ND-1.21 g/ Kg and 0.34 g/kg in dried meat, ND-0.84 g/Kg and 0.12 g/Kg in bacon, ND-1.27 g/Kg and 0.074 g/Kg in ham, ND- 0.90 g/Kg and 0.077 g/Kg in sausage, and ND-0.20 g/Kg, 0.015 g/Kg in can meat. Together, these results demonstrated that the processed market meat products must be reinforced to supply for the citizens as safe foods.

Microbiological Studies of Foods - Microbial Load and Microflora of Dried File Fish Fillet - (식품(食品)의 미생물학적(微生物學的) 조사연구(調査硏究) - 시판(市販) 쥐치포의 미생물(微生物) 분포(分布) -)

  • Yoo, Jin Young;Chung, Dong Hyo;Kim, Jun Pyong
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.1-5
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    • 1985
  • File fish fillets collected from markets were analyzed for their microbial load and microflora, resulting in the level of aerobic mesophiles ranged from $3.5{\times}10^3$ to $1.1{\times}10^8CFU/g$, total coliforms from less than 2.3 to $4.6{\times}10^5MPN/g$, fecal coliforms from 1ess than 2.3 to $1.1{\times}10^5MPN/g$, Enterobacteriaceae from $3{\times}10$ to $4.4{\times}10^5CFU/g$ and fecal Streptococci from $1.6{\times}10^2$ to $7.6{\times}10^6CFU/g$ having Staphylococcus hominis, Staphylococcus hemolyticus, Micrococcus varians, Micrococcus luteus and Streptococcus cremoris as constituent microorganisms of aerobic mesophiles, Escherichia coli and Proteus rettgeri of Family Enterobacteriaceae, and Streptococcus faecium and Streptococcus avium of fecal Streptococci.

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Grazing on Bacteria and Algae by Metazoans in the Lake-river Ecosystem (River Spree, Germany)

  • Kim, Hyun-Woo;Joo, Gea-Jae;Walz, Norbert
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.111-115
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    • 2008
  • Direct effects of zooplankton grazing activities on the natural assemblage of bacterioplankton and algae were evaluated at monthly intervals, from June to October of 2000, in the middle part of the River Spree, Germany. We quantified bacterioplankton, algae, zooplankton abundance and measured carbon ingestion rates (CIRs) by zooplankton according to two zooplankton size classes: (i) micro zooplankton (MICZ), ranging in size from 30 to $150{\mu}m$ and including rotifers and nauplii, excluding protozoans and (ii) macrozooplankton (MACZ), larger than $150{\mu}m$ and including cladocerans and copepods. CIRs were measured using natural bacterial and algae communities in the zooplankton density manipulation experiments. Algae biomass (average${\pm}$SD: $377{\pm}306{\mu}gC\;L^{-1}$, n=5) was always higher than bacterial biomass ($36.7{\pm}9.9{\mu}gC\;L^{-1}$, n=5). Total zooplankton biomass varied from 19.8 to $137{\mu}gC\;L^{-1}$. Total mean biomass of zooplankton was $59.9{\pm}52.5{\mu}gC\;L^{-1}$ (average${\pm}$SD, n=5). Average MICZ biomass ($40.2{\pm}47.6{\mu}gC\;L^{-1}$ n=5) was nearly twofold higher than MACZ biomass ($19.6{\pm}20.6{\mu}gC\;L^{-1}$ n=5). Total zooplankton CIRs on algae (average${\pm}$SD: $56.6{\pm}26.4{\mu}gC\;L^{-1}\;day^{-1}$) were $\sim$fourfold higher than that on bacteria $(12.7{\pm}6.0{\mu}gC\;L^{-1}\;day^{-1})$. MICZ CIRs on bacteria $(7.0{\pm}2.8{\mu}gC\;L^{-1}\;day^{-1})$ and algae $(28.6{\pm}20.6{\mu}gC\;L^{-1}\;day^{-1})$ were slightly higher than MACZ CIRs. On average, MICZ accounted for 55.6 and 50.5% of total zooplankton grazing on bacteria and algae, respectively. Considering the MICZ and MACZ CIRs, the relative role of transferring carbon to higher trophic levels were nearly similar between both communities in the lake-river ecosystem.

Clinical Findings of Genotypes in Korean Patients with Glycogen Storage Disease Type Ia (한국인 당원병 제 Ia형에서 유전형의 임상 양상)

  • Ko, Jae Sung;Yang, Hye Ran;Kim, Jong Won;Seo, Jeong Kee
    • Clinical and Experimental Pediatrics
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    • v.48 no.8
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    • pp.877-880
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    • 2005
  • Purpose : Glycogen storage disease type Ia(GSD Ia) is an autosomal recessive disorder caused by the deficiency of glucose-6-phosphatase(G6Pase). The aim of the study was to investigate the spectrum of G6Pase gene mutations and relationship between genotype and clinical findings in Korean patients with GSD Ia. Methods : Genomic DNA was extracted from peripheral leukocytes of 20 patients with GSD Ia. The five exons of G6Pase gene were amplified and PCR products were directly sequenced. The frequency of short stature, hypoglycemia, hypercholesterolemia, hyperuricemia, hypercalciuria, nephrocalcinosis and hepatic adenoma was compared between 727G>T homozygotes and 727G>T compound heterozygotes. Results : A total of 5 different mutations were identified. The most common mutation was the 727G>T with an allele frequency of 80%. All patients were either homozygous(12/20) or heterozygous(8/20) for the 727G>T mutation. G122D was found in 3 patients, P178A in 1, G222R in 2, and S339R in 2. There was no difference in the frequency of short stature, hypoglycemia, hypercholesterolemia, hyperuricemia, nephrocalcinosis, and hepatic adenoma between 727G>T homozygotes and heterozygotes. Conclusion : Diagnosis of GSD Ia can be based on clinical and biochemical abnormalities combined with mutation analysis instead of enzymatic diagnosis that requires liver biopsy. Homozygosity for the 727G>T does not seem to alter the disease phenotype as compared with the heterozygous state.

Physico-chemical Properties of Soils Developed on the Different Topographies in Korea (우리나라 농경지토양(農耕地土壤)의 지형별(地形別) 이화학적(理化學的) 특성(特性))

  • Hyeon, Geun-Soo;Park, Chang-Seo;Jung, Sug-Jae;Moon, Joon
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.4
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    • pp.271-279
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    • 1989
  • Mean values representing the particle size distribution and some chemical properties for the cultivated soils were obtained from the analysis results of the typical profiles, which were described by the detailed soil maps throughout Korea. Analysis results of 5,215 soil samples (3,075 for paddy and 2,140 for upland) were available for the determination of mean values. The results are under. 1. Paddy topsoil contained 20.4% for clay, 5.8 for pH, 2.6% for organic matter, 10.4me/100g for exchangeable K, and 89ppm for available $P_2O_5$. Upland topsoil did 17.3% for clay, 5.5 for pH, 1.8% for organic matter, 9.lme/100g for CEC, 0.29me/100g for exchangeable K, and 103ppm for availabal $P_2O_5$. 2. Soil properies for paddy were markedly influenced by the reliefs. Topsoil contained 21.4% for clay, 6.0 for pH, 2.2% for organic matter, 10.8me/100g for CEC, 0.39me/100g for exchang-cable K and 57ppm for available $P_2O_5$ on the fluvio-marine plain, 15.3%, 5.7, 2.0%, 8.6me/100g, 0.17me/100g and 76ppm on the alluvial plain, 18.8%, 5.9, 2.7%, 10.4me/100g, 0.19me/100g and 80ppm on the valleys and fans, 25.0%, 5.7, 2.5%, 11.5me/100g, 0.26me/100g, 0.27me/100g and 141ppm on the moutain foot slopes, respectively. 3. Soil Properties for upland, also, were markedly influenced by the reliefs. Topsoil contained 5.5% for clay, 5.7 for pH, 1.1% for organic matter, 4.7me/100g for CEC, 0.17me/100g for exchangeable K and 50ppm for available $P_2O_5$ on the fluvio-marine plain, 10.3%, 5.5, 1.4%, 7.6me/100g, 0.26me/100g and 160ppm on the alluvial plain, 13.9%, 5.4, 1.8%, 9.3me/100g, 0.24me/100g and and 184ppm on the valleys and fans, 29.8%, 5.3, 2.1%, 11.2me/100g 0.40me/100g and 58ppm on the alluvial plain, 20.0%, 5.7, 2.7%, 11.4me/100g, 0.32me/100g and 116ppm on the mountain foot slopes, and 24.6%, 5.3, 1.8%, 10.2me/100g, 0.28me/100g and 51ppm on the rolling and Hill. 4. All chemical properties did not reach the ideal value for maximizing land capability. 5. Organic matter, exchangeable cations and available $P_2O_5$ were not normally distributed. Intervals of one and two standard deviations about mean of an approximately normal distribution were calculated.

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Syntheses and Biological Activities of Uridine Nucleoside Derivatives (Uridine Nucleoside 유도체의 합성과 생물 활성)

  • Bong-Hun Lee;Jang-Su Park;Shin-Won Kang
    • Journal of Life Science
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    • v.9 no.1
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    • pp.63-68
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    • 1999
  • Many nucleoside compounds such as 5-halogen substituted uridine, 5'-amino-5'-deoxyuridine conjugates of amino acid, peptide, and penicillin G, 5'-monophosphate uridine derivatives and 5'-monophosphate uridine-fatty acid derivatives were chemically synthesized and their antifungal, antibacterial, and antitumor activities were tested. 5-Bromo-2',3'-O-isopropylideneuridine(6) inhibited the growth of Trichophyton rubrum at $0.2{\mu}$g/ml of MIC. 5'-Amino-5'-deoxyuridine-penicillin G(19), 5'-amino-5'-deoxyuridine-cyclo(Phe-Asp)(20), and 5-iodo-5'-amino-5'-deoxyuridine- penicillin G(22) had antibarterial activity(MIC was $6.25{\mu}$g/ml against S. aureus) and the latter two nucleoside compounds were the most antitumor derivatives(their $IC_{50}$ against L5178Y murine lymphoma cell was $6.5{\mu}$g/ml).

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Comparison of in vitro Antimicrobial Activities of Tc-99m Infecton and Ciprofloxacin (Tc-99m Infecton과 Ciprofloxacin의 생체외 항균력 비교)

  • Kim, Sung-Min;Bom, Hee-Seung;Song, Ho-Chun;Jeong, Hwan-Jeong;Kim, Ji-Yeul;Shin, Jong-Hee
    • The Korean Journal of Nuclear Medicine
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    • v.35 no.1
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    • pp.75-80
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    • 2001
  • Purpose: There was little evidence that Tc-99m labeled ciprofloxacin (Infecton) located inside of bacteria. Antimicrobial activity of Infecton could be an indirect evidence of its location. We compared in vitro antimicrobial activities of Infecton and ciprofloxacin. Materials and methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Infecton and ciprofloxacin against three standard strains of bacteria, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were measured using modified broth macrodilution techniques and pour plate methods, respectively. Data were expressed as mean${\pm}$SE (range). Results: MICs of Infecton and ciprofloxacin were $1.12{\pm}0.20\;(0.8{\sim}1.6){\mu}g/ml\;and\;0.35{\pm}0.04\;(0.2{\sim}0.4){\mu}g/ml$ for S. aureus, $0.03{\pm}0.005\;(0.025{\sim}0.05){\mu}g/ml\;and\;0.011{\pm}0.001\;(0.006{\sim}0.012){\mu}g/ml$ for E. coil, and $0.96{\pm}0.16\;(0.8{\sim}1.6){\mu}g/ml)\;and\;0.56{\pm}0.098\;(0.4{\sim}0.8){\mu}g/ml$ for P. aeruginosa, respectively. MBCs of Infecton and ciprofloxacin were $2.56{\pm}0.39\;(1.6{\sim}3.2){\mu}g/ml\;and\;0.88{\pm}0.2\;(0.4{\sim}1.6){\mu}g/ml$ for S. aureus, $0.04{\pm}0.05\;(0.025{\pm}0.05){\mu}g/ml\;and\;0.02{\pm}0.01\;(0.025{\sim}0.05)\;{\sim}g/ml$ for E coli, and $2.24{\pm}0.39\;(1.6{\sim}3.2){\mu}g/ml\;and\;1.44{\pm}0.16\;(0.8{\sim}1.6){\mu}g/ml$ for P. aeruginosa, respectively. Conclusion: Although both MICs and UBCs of Infecton were higher than those of ciprofloxacin, all three standard bacterial strains were sensitive to Infecton. It could be an indirect evidence that Tc-99m Infecton be a specific imaging agent for bacterial infection.

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Study of Trans Fatty Acids and Saturated Fatty Acids in Child-favored Foods (어린이 기호식품 중 트랜스지방 및 포화지방 실태조사)

  • Yoon, Tae-Hyung;Lee, Sung-Min;Shin, Hee-Jun;Lee, Soo-Yeon;Hong, Jin;No, Ki-Mi;Park, Kyoung-Sik;Leem, Dong-Gil;Lee, Kwang-Ho;Jeong, Ja-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1562-1568
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    • 2011
  • We studied the amounts of trans fatty acids and saturated fatty acids in child-favored foods. Confectioneries, breads, donuts and hamburgers were included in this study. The total fatty acid content and the amount of each type of fatty acid were determined by gas chromatography with a flame-ionized detector. Confectioneries were shown to have the highest content of crude fat. The mean content was 24.2${\pm}$6.9 (range: 4.6~41.1) g/100 g food. The mean content of crude fat in donuts, bread and hamburgers was 23.9${\pm}$5.8 (range: 14.1~39.5) g/100 g food, 15.7${\pm}$7.9 (range: 1.4~30.0) g/100 g food, and 9.5${\pm}$3.4 (range: 4.5~18.5) g/100 g food, respectively. Bread had the most trans fatty acids at 1.3 g/100 g food. This result inferred that manufacturers have tried to reduce the trans fat content. The mean content of saturated fatty acids in confectioneries, donuts, bread, and hamburgers was 11.6${\pm}$4.8 (range: 2.0~22.7) g/100 g food, 11.2${\pm}$4.0 (range: 4.8~23.2) g/100 g food, 6.9${\pm}$4.1 (range: 0.6~15.4) g/100 g food, 3.0${\pm}$1.0 (range: 1.0~5.8) g/100 g food, respectively. This content depended on crude fat. The composition of fatty acids varied according to the oil and fat used in the manufacturing process of each food, and the natural content. Foods that were fried in vegetable oil tended to be especially low in saturated fat.

Mass balance of the phosphorus and nitrogen in variable input concentration and fertilization in cropping rice (수도재배에서 유입수의 농도와 시비량의 변화에 의한 질소, 인의 Mass Balance(지역환경 \circled3))

  • 황하선;윤춘경
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2000.10a
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    • pp.623-628
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    • 2000
  • This study were conducted to evaluate the mass balance of phosphorus and nitrogen with cropping in experimental pot where the mass of input concentration, and fertilization were variable. Four treatments include CSWNF, TWCF, SWNF. And these cases were compared to the control case of tap water irrigation with conventional fertilization (CONTROL). Fertilization were following conventional fertilization , N : P : K = 11kg : 7kg : 8kg. Input water loading was CSWNF (N:48.7g ,P:3.6g), TWCF(7.8g, 0.6g), SWHF(38.8g ,2.9g), TWNF(38.8g, 2.9g ) and CONTROL(0g ,0g) The result is nitrogen decrease rate; TWCF(19.2%), SWHF(14.9%), CSWNF(9.2%) and SWCF(5.6%). phosphorous decrease rate ; TWCF (10%), SWHF(3.7%), SWCF(0.9%) and CSWNF(0.3%).

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Analysis of Manganese Contents in 30 Korean Common Foods (한국인 상용식품 중 30종류 식품의 망간 함량 분석)

  • 최미경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1408-1413
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    • 2003
  • This study was conducted to analyze manganese contents of Korean common foods. Contents of manganese in 30 foods were analyzed by ICP spectrometer. And daily manganese intake through 30 common foods was calculated using analysis data of this study and daily food intakes cited from report on 1998 national health and nutrition survey. The average manganese contents of foods analyzed were 949.6 $\mu\textrm{g}$ for rice, 236.1 $\mu\textrm{g}$ for Korean chinese cabbage kimchi, 27.2 $\mu\textrm{g}$ for citrus fruit, 2.6 $\mu\textrm{g}$ for milk, 214.6 $\mu\textrm{g}$ for radish root, 40.0 $\mu\textrm{g}$ for apple, 60.4 $\mu\textrm{g}$ for persimmon, 13.9 $\mu\textrm{g}$ for pork, 9.5 $\mu\textrm{g}$ for beef, 638.3 $\mu\textrm{g}$ for soybean curd, 184.0 $\mu\textrm{g}$ for radish kimchi, 56.0 $\mu\textrm{g}$ for pear, 18.4 $\mu\textrm{g}$ for beer, 11.3 $\mu\textrm{g}$ for egg, 9.5 $\mu\textrm{g}$ for carbonated beverage, 345.0 $\mu\textrm{g}$ for bread, 50.7 $\mu\textrm{g}$ for soju, 270.3 $\mu\textrm{g}$ for potato, 236.1 $\mu\textrm{g}$ for sweet potato, 91.2 $\mu\textrm{g}$ for ramyeon, 32.5 $\mu\textrm{g}$ for onion, 68.0 $\mu\textrm{g}$ for nabak kimchi, 538.2 $\mu\textrm{g}$ for soybean sprout, 112.5 $\mu\textrm{g}$ for welsh onion, 336.7 $\mu\textrm{g}$ for rice cake, 589.9 $\mu\textrm{g}$ for Korean chinese cabbage, 430.4 $\mu\textrm{g}$ for somyeon, 144.3 $\mu\textrm{g}$ for pumpkin, 3.0 $\mu\textrm{g}$ for yoghurt, and 614.4 $\mu\textrm{g}$ for spinach per 100 g of each food. The daily manganese intake through 30 common foods of Koreans in 1998 was 3420.7 $\mu\textrm{g}$. Major sources of dietary manganese were rice, kimchi, and soybean curd. Especially, rice supplied 68.1% of total dietary manganese intake through 30 common foods. Further studies are required to establish database and RDA of manganese.