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Effects of a Tetramethoxyhydroxyflavone on the Expression of Inflammatory Mediators in LPS-Treated Human Synovial Fibroblast and Macrophage Cells

  • Yoon, Do-Young;Cho, Min-Chul;Kim, Jung-Hee;Kim, Eun-Jin;Kang, Jeong-Woo;Seo, Eun-Hee;Shim, Jung-Hyun;Kim, Soo-Hyun;Lee, Hee-Gu;Oh, Goo-Taeg;Hong, Jin-Tae;Park, Joo-Won;Kim, Jong-Wan
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.686-694
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    • 2008
  • The inhibitory effects of 5,6,3',5'-tetramethoxy 7,4'-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1${\beta}$ or TNF-${\alpha}$ induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin $E_2$ in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1${\beta}$ and thus proliferates), revealing that p7F inhibited IL-1${\beta}$-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IkB degradation and NF-${\kappa}$B activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.

Molecular Theory of Plastic Deformation (Ⅲ)$^*$

  • Kim, Jae-Hyun;Ree, Tai-Kyue;Kim, Chang-Hong
    • Bulletin of the Korean Chemical Society
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    • v.2 no.3
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    • pp.96-104
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    • 1981
  • (1) The flow data of f (stress) and ${\dot{s}$ (strain rate) for Fe and Ti alloys were plotted in the form of f vs. -ln ${\dot{s}$ by using the literature values. (2) The plot showed two distinct patterns A and B; Pattern A is a straight line with a negative slope, and Pattern B is a curve of concave upward. (3) According to Kim and Ree's generalized theory of plastic deformation, pattern A & B belong to Case 1 and 2, respectively; in Case 1, only one kind of flow units acts in the deformation, and in Case 2, two kinds flow units act, and stress is expressed by $f={X_1f_1}+{X_2f_2}$where $f_1\;and\;f_2$ are the stresses acting on the flow units of kind 1 and 2, respectively, and $X_1,\;X_2$ are the fractions of the surface area occupied by the two kinds of flow units; $f_j=(1/{\alpha}_j) sinh^{-1}\;{\beta}_j{{\dot{s}}\;(j=1\;or\;2)$, where $1/{\alpha}_j\;and\;{\beta}_j$ are proportional to the shear modulus and relaxation time, respectively. (4) We found that grain-boundary flow units only act in the deformation of Fe and Ti alloys whereas dislocation flow units do not show any appreciable contribution. (5) The deformations of Fe and Ti alloys belong generally to pattern A (Case 1) and B (Case 2), respectively. (6) By applying the equations, f=$(1/{\alpha}_{g1}) sinh^-1({\beta}_{g1}{\dot{s}}$) and $f=(X_{g1}/{\alpha}_{g1})sinh^{-1}({\beta}_{g1}{\dot{s}})+ (X_{g2}/{\alpha}_{g2})\;shih^{-1}({\beta}_{g2}{\dot{s}})$ to the flow data of Fe and Ti alloys, the parametric values of $x_{gj}/{\alpha}_{gj}\;and\;{\beta}_{gs}(j=1\;or\;2)$ were determined, here the subscript g signifies a grain-boundary flow unit. (7) From the values of ($({\beta}_gj)^{-1}$) at different temperatures, the activation enthalpy ${\Delta}H_{gj}^{\neq}$ of deformation due to flow unit gj was determined, ($({\beta}_gj)^{-1}$) being proportional to , the jumping frequency (the rate constant) of flow unit gj. The ${\Delta}H_{gj}\;^{\neq}$ agreed very well with ${\Delta}H_{gj}\;^{\neq}$ (self-diff) of the element j whose diffusion in the sample is a critical step for the deformation as proposed by Kim-Ree's theory (Refer to Tables 3 and 4). (8) The fact, ${\Delta}H_{gj}\;^{\neq}={\Delta}H_{j}\;^{\neq}$ (self-diff), justifies the Kim-Ree theory and their method for determining activation enthalpies for deformation. (9) A linear relation between ${\beta}^{-1}$ and carbon content [C] in hot-rolled steel was observed, i.e., In ${\beta}^{-1}$ = -50.2 [C] - 40.3. This equation explains very well the experimental facts observed with regard to the deformation of hot-rolled steel..

A Study On the Operating Room Nurses' Performances of Duties and Their Fatigue (수술실간호사의 직무수행과 피로에 관한 연구)

  • Park, Jeong Sook
    • Korean Journal of Occupational Health Nursing
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    • v.6 no.2
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    • pp.110-127
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    • 1997
  • The aim of this study is to present the basic datum for the promotion of effectiveness and improvement of nursery tasks, by grasping the situation of operating room nurses tasks and the degree of their fatigue. For these researches, 70 nurses out of the operating rooms of 3 university-affiliated hospitals-two in Seoul and one in Pusan, were chosen at random by a non-probability sampling method. These researches were done from April 14 to April 26 in 1997 by questionairing method. The questionaire was composed of 30 items, which asks the examinees of their physical, mental, and neurosensory symptoms, with 10 items respectively. The reliability of the research instrument was turned out very high with Cronbach's ${\alpha}=.9376$. The datum were electronically processed using Statistics Program for Social Sciences(SPSS). The analysis of datum in this study has a general character, in which the demosociological character and the special ex-officio character was calculated by frequency and percentage. The situation of tasks in operating rooms was calculated by frequency and percentage. The fatigue of operating room nurses was calculated by average and standard deviation. To compare the fatigue with regard to the character of operating room nurse's tasks, t-test and F-test(ANOVA) were used after the character of variations, and the variations at the level of P<.05 which might have some meaning was verified after the fact with Duncan's Multiple Range(DMR). The results of this research are as follows : 1) The nurses working in operating rooms show their fatigue in three fields-in Group I physical symptoms 3.28, in Group III neurosensory symptoms 2.85, and in Group II mental symptoms 2.73, which shows I Dominant type (general type). 2) They complain, in Group I they are "feeling the heavy legs," 3.28 and in Group II they "occasionally forget soon what to do," 3.09. and in Group III, they "feel lumbago," 3.47, which is the highest rate of the three. The highest rate results from the character of their tasks, in which they have to move rapidly the heavy appliances and do their jobs standing many hours, especially wearing heavy radiation protector. 3) As to transportation, subway using group feel the greatest fatigue, 3.18(F=4.315, P=.008). 4) As to department, nurses in the orthopedic's surgery part feel the greatest fatigue, 3.26(F=2.040, P=.050). 5) As to the change of physical symptoms, the group answering that they found physical abnormality after working in operating rooms show the greatest fatigue, 3.12(t=-3.13, P=.003). 6) At to the general circumstances, the group answering that they receive insufficient consideration on their physical abnormality in their department, show the greatest fatigue, 3.10(F=3.200, P=0.47). 7) As to the relation with superior officers, the group answering that their superior officer has an impetuous temperament, show great fatigue, 3.11(F=4.855, P=.011). 8) As to the time of feeling fatigue, the fatigue reaches the highest point 1~2 hours after operations, 3.04(F=2.703, P=.046). 9) When they feel fatigue after scrub nurse duties, they feel the greatest fatigue 2 hours after the duties, 3.09( F=2.841, P=.038). 10) As to the operation instruments, when they use complex instruments borrowing from the outside in addition to the basic instruments, their fatigue becomes the greatest, 3.09(F=7.831, P=.000). 11) As to the kind of operation, when they participate in orthopedic's surgery operations, they feel the greatest fatigue, 3.18(F=4.362, P=.000). With the above results, it is proved that the degree of operating room nurses' fatigue is considerably high. So it may be concluded that the measure for lessening the fatigue should be find immediately, not on the level of personal matters but on the level of hospital nursing administration.

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Enhancing Classification Performance of Temporal Keyword Data by Using Moving Average-based Dynamic Time Warping Method (이동 평균 기반 동적 시간 와핑 기법을 이용한 시계열 키워드 데이터의 분류 성능 개선 방안)

  • Jeong, Do-Heon
    • Journal of the Korean Society for information Management
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    • v.36 no.4
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    • pp.83-105
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    • 2019
  • This study aims to suggest an effective method for the automatic classification of keywords with similar patterns by calculating pattern similarity of temporal data. For this, large scale news on the Web were collected and time series data composed of 120 time segments were built. To make training data set for the performance test of the proposed model, 440 representative keywords were manually classified according to 8 types of trend. This study introduces a Dynamic Time Warping(DTW) method which have been commonly used in the field of time series analytics, and proposes an application model, MA-DTW based on a Moving Average(MA) method which gives a good explanation on a tendency of trend curve. As a result of the automatic classification by a k-Nearest Neighbor(kNN) algorithm, Euclidean Distance(ED) and DTW showed 48.2% and 66.6% of maximum micro-averaged F1 score respectively, whereas the proposed model represented 74.3% of the best micro-averaged F1 score. In all respect of the comprehensive experiments, the suggested model outperformed the methods of ED and DTW.

Attitude to Reproductive health, Value of Marriage and Children of University students (대학생의 생식건강에 대한 태도, 결혼관 및 자녀관)

  • Kim, Hyun;Hong, YoungSun
    • The Journal of Korean Society for School & Community Health Education
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    • v.14 no.1
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    • pp.13-24
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    • 2013
  • Background & Objectives: The aim of the study was to investigate characteristics and relationship among University students' attitude to reproductive health, value of marriage and children Methods: With a descriptive survey design, a self-report study was conducted and collected 470 responses from university students in Chungcheongnam-Do. Descriptive statistics, t-test, ANOVA, and Pearson's correlation coefficients were used to analyze data. Results: It was found that there were significantly different in attitude to reproductive health according to the following variables: age(F=2.91, p<.05), college(F=2.49, p<.05), sexual experience(t=7.54, p<.001). value of marriage was significantly different according to gender(t=-7.05, p<.001), college(F=4.42, p<.05), plan to marriage(t=-5.58, p<.001) and plan to having children(t=-4.30, p<.001). Value of children was significantly different according to gender(t=4.52, p<.001), plan to marriage(t=-4.30, p<.001) and plan to having children(t=6.33, p<.001). Attitude to reproductive health was significantly correlated with value of marriage(t=6.33, p<.001), value of marriage was significantly correlated with value of child(r=.224, p<.01). Conclusion: These results suggest that education for improving attitude to reproductive health, value of marriage and child are necessary to overcome low fertility. In addition, it is needed to further research and at the national level policy.

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Dependence of the 1/f Noise Characteristics of CMOSFETs on Body Bias in Sub-threshold and Strong Inversion Regions

  • Kwon, Sung-Kyu;Kwon, Hyuk-Min;Kwak, Ho-Young;Jang, Jae-Hyung;Shin, Jong-Kwan;Hwang, Seon-Man;Sung, Seung-Yong;Lee, Ga-Won;Lee, Song-Jae;Han, In-Shik;Chung, Yi-Sun;Lee, Jung-Hwan;Lee, Hi-Deok
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.13 no.6
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    • pp.655-661
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    • 2013
  • In this paper, the 1/f noise characteristics of n-channel MOSFET (NMOSFET) and p-channel MOSFET (PMOSFET) are analyzed in depth as a function of body bias. The normalized drain current noise, $S_{ID}/I_D{^2}$ showed strong dependence on the body bias in the sub-threshold region for both NMOSFET and PMOSFET, and NMOSFET showed stronger dependence than PMOSFET on the body bias. On the contrary, both of NMOSFET and PMOSFET do not exhibit the dependence of $S_{ID}/I_D{^2}$ on body bias in strong inversion region, although the noise mechanisms of two MOSFETs are different from each other.

A STUDY ON THE CHANGES OF THE ROOT SURFACE PLAQUE FLORA AND GINGIVAL CREVICULAR FLUID ANTIBODY TITERS AFTER ANTIMICROBIAL VARNISH TREATMENT (치근면에 항균 varnish 처치 전후의 치태 세균 및 치은열구액내 항체수준 변화에 관한 연구)

  • Do, Jeong-Wook;Kwon, Hyuk-Choon
    • Restorative Dentistry and Endodontics
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    • v.18 no.2
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    • pp.341-356
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    • 1993
  • In the prevention of root surface caries, antimicrobial therapy for the control of subgingival and supragingival plaque is seriously considered as a long term suppression of pathogenic microflora. Recently, varnishes containing antimicrobial agents have been developed to control the supragingival microflora. The purpose of this study was to determine the antimicrobial effects of 20% chlorhexidine varnish and 2.6% silane fluoride varnish with sealant. In clinical experiments, 12 subjects were selected from the periodontally treated patient and divided into 3 groups. After a dental prophylaxis, the subjects were treated with single application of placebo varnish (group I), 20% chlorhexidine varnish (group II), and 2.6% silane fluoride varnish (group III). Root surface plaque samples were taken before (baseline) and one, two, four, and 8 weeks after the treatments. Microbiological examinations of root surface plaque were performed with culture study and indirect immunoflorescence (I.I.F.) study, and immunological examination of gingival crevicular fluid antibody titers was performed with ELISA study. The results were as follows: 1. Pathogenic microflora on the root surface including S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus were 24 - 37% on I.I.F. study. 2. S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus of the root surface plaque was significantly reduced from 1 week to 8 weeks after antimicrobial varnish treatment, but showed generally increasing tendency in control group. 3. Gingival crevicular fluid antibody titers were significantly reduced from 1 or 2 weeks to 4 weeks after antimicrobial varnish treatment.

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The Relation between Obesity among Middle School Students and Total Cholesterol, Blood Glucose and Blood Pressure (일 중소도시 중학생의 비만과 총콜레스테롤, 혈당, 혈압간의 관계)

  • Kim Eun Young
    • Journal of Korean Public Health Nursing
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    • v.19 no.1
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    • pp.5-17
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    • 2005
  • This research was conducted to determine the actual condition of obesity and obese students' total cholesterol. blood glucose and blood pressure, and to analyze the relationship between the obesity rate and total cholesterol. blood glucose and blood pressure among the students at 7 middle schools in A city in Kyoung ki-do. The results were as follows: 1. Among 12,148 student participants in this investigation. students of normal weight range were $91.5\%$ and obese students were $8.5\%$, breaking down to mildly obese of $4.3\%$ $(boys,\;5.4\%;\;girls,\;3.0\%)$. moderately obese of $3.4\% (boys,\;4.6\%;\;girls,\;2.1\%)$ and severely obese of $0.7\%(boys,\;0.9\%;\;girls,\;0.5\%)$, The gender difference was statistically significant $(x^2=111.5830. p=.0001). 2. To analyze the 1.027 obese students. the average of total cholesterol was 166.9mg/dl, 171.0mg/dl and 182.1mg/dl in the mild, moderate and severe obesity groups, respectively. The results became more significant with increasing obesity rate (F=10.06, p=.0001). The average of systolic blood pressure (SBP) was 121.9mmHg, 123.2mm, and 127.5mm, respectively. The results became more significant with increasing obesity rate (F=6.29, p=.0019). The average of diastolic blood pressure (DBP) was 74.4mmHg. 76.0mmHg. and 78.4mmHg, respectively. The results became more significant with increasing obesity rate (F=8.15. p=.0003). The average of blood glucose was 83.3mg/dl, 84.5mg/dl, and 82.3mg/dl, respectively. There was no significant difference with obesity rate. 3. There were significant correlations between obesity rate and cholesterol(r=.11288, p=.0003), between obesity rate and DBP(r=.14209, p=.000l). and between obesity rate and SBP(r=.14081. p=.0001). However, there was no significant correlation between obesity rate and blood glucose (r=.00655, p=.8339).

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Characteristics of Memory Windows of MFMIS Gate Structures (MFMIS 게이트 구조에서의 메모리 윈도우 특성)

  • Park, Jun-Woong;Kim, Ik-Soo;Shim, Sun-Il;Youm, Min-Soo;Kim, Yong-Tae;Sung, Man-Young
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.07a
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    • pp.319-322
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    • 2003
  • To match the charge induced by the insulators $CeO_2$ with the remanent polarization of ferro electric SBT thin films, areas of Pt/SBT/Pt (MFM) and those of $Pt/CeO_2/Si$ (MIS) capacitors were ind ependently designed. The area $S_M$ of MIS capacitors to the area $S_F$ of MFM capacitors were varied from 1 to 10, 15, and 20. Top electrode Pt and SBT layers were etched with for various area ratios of $S_M\;/\;S_F$. Bottom electrode Pt and $CeO_2$ layers were respectively deposited by do and rf sputtering in-situ process. SBT thin film were prepared by the metal orgnic decomposition (MOD) technique. $Pt(100nm)/SBT(350nm)/Pt(300nm)/CeO_2(40nm)/p-Si$ (MFMIS) gate structures have been fabricated with the various $S_M\;/\;S_F$ ratios using inductively coupled plasma reactive ion etching (ICP-RIE). The leakage current density of MFMIS gate structures were improved to $6.32{\times}10^{-7}\;A/cm^2$ at the applied gate voltage of 10 V. It is shown that in the memory window increase with the area ratio $S_M\;/\;S_F$ of the MFMIS structures and a larger memory window of 3 V can be obtained for a voltage sweep of ${\pm}9\;V$ for MFMIS structures with an area ratio $S_M\;/\;S_F\;=\;6$ than that of 0.9 V of MFS at the same applied voltage. The maximum memory windows of MFMIS structures were 2.28 V, 3.35 V, and 3.7 V with the are a ratios 1, 2, and 6 at the applied gate voltage of 11 V, respectively. It is concluded that ferroelectric gate capacitors of MFMIS are good candidates for nondestructive readout-nonvolatile memories.

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Changing Trends of Climatic Variables of Agro-Climatic Zones of Rice in South Korea (벼 작물 농업기후지대의 연대별 기후요소 변화 특성)

  • Jung, Myung-Pyo;Shim, Kyo-Moon;Kim, Yongseok;Kim, Seok-Cheol;So, Kyu-Ho
    • Journal of Climate Change Research
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    • v.5 no.1
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    • pp.13-19
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    • 2014
  • In the past, Korea agro-climatic zone except Jeju-do was classified into nineteen based on rice culture by using air temperature, precipitation, and sunshine duration etc. during rice growing periods. It has been used for selecting safety zone of rice cultivation and countermeasures to meteorological disasters. In this study, the climatic variables such as air temperature, precipitation, and sunshine duration of twenty agro-climatic zones including Jeju-do were compared decennially (1970's, 1980's, 1990's, and 2000's). The meteorological data were obtained in Meteorological Information Portal Service System-Disaster Prevention, Korea Meteorological Administration. The temperature of 1970s, 1980s, 1990s, and 2000s were $12.0{\pm}0.14^{\circ}C$, $11.9{\pm}0.13^{\circ}C$, $12.2{\pm}0.14^{\circ}C$, and $12.6{\pm}0.13^{\circ}C$, respectively. The precipitation of 1970s, 1980s, 1990s, and 2000s were $1,270.3{\pm}20.05mm$, $1,343.0{\pm}26.01mm$, $1,350.6{\pm}27.13mm$, and $1,416.8{\pm}24.87mm$, respectively. And the sunshine duration of 1970s, 1980s, 1990s, and 2000s were $421.7{\pm}18.37hours$, $2,352.4{\pm}15.01hours$, $2,196.3{\pm}12.32hours$, and $2,146.8{\pm}15.37hours$, respectively. The temperature in Middle-Inland zone ($+1.2^{\circ}C$) and Eastern-Southern zone ($+1.1^{\circ}C$) remarkably increased. The temperature increased most in Taebak highly Cold zone ($+364mm$) and Taebak moderately Cold Zone ($+326mm$). The sunshine duration decreased most in Middle-Inland Zone (-995 hours). The temperature (F=2.708, df=3, p= 0.046) and precipitation (F=5.037, df=3, p=0.002) increased significantly among seasons while the sunshine duration decreased significantly(F=26.181, df=3, p<0.0001) among seasons. In further study, it will need to reclassify agro-climatic zone of rice and it will need to conduct studies on safe cropping season, growth and developing of rice, and cultivation management system etc. based on reclassified agro-climatic zone.