• Title/Summary/Keyword: 62G05

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Anti-Melanogenic Effect of Oenothera laciniata Methanol Extract in Melan-a Cells

  • Kim, Su Eun;Lee, Chae Myoung;Kim, Young Chul
    • Toxicological Research
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    • v.33 no.1
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    • pp.55-62
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    • 2017
  • We evaluated the antioxidant activity and anti-melanogenic effects of Oenothera laciniata methanol extract (OLME) in vitro by using melan-a cells. The total polyphenol and flavonoid content of OLME was 66.3 and 19.0 mg/g, respectively. The electron-donating ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity, and superoxide dismutase (SOD)-like activity of OLME ($500{\mu}g/mL$) were 94.5%, 95.6%, and 63.6%, respectively. OLME and arbutin treatment at $50{\mu}g/mL$ significantly decreased melanin content by 35.5% and 14.2%, respectively, compared to control (p < 0.05). OLME and arbutin treatment at $50{\mu}g/mL$ significantly inhibited intra-cellular tyrosinase activity by 22.6% and 12.6%, respectively, compared to control (p < 0.05). OLME ($50{\mu}g/mL$) significantly decreased tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor-M (MITF-M) mRNA expression by 57.1%, 67.3%, 99.0%, and 77.0%, respectively, compared to control (p < 0.05). Arbutin ($50{\mu}g/mL$) significantly decreased tyrosinase, TRP-1, and TRP-2 mRNA expression by 24.2%, 42.9%, and 48.5%, respectively, compared to control (p < 0.05). However, arbutin ($50{\mu}g/mL$) did not affect MITF-M mRNA expression. Taken together, OLME showed a good antioxidant activity and anti-melanogenic effect in melan-a cells that was superior to that of arbutin, a well-known skin-whitening agent. The potential mechanism underlying the anti-melanogenic effect of OLME was inhibition of tyrosinase activity and down-regulation of tyrosinase, TRP-1, TRP-2, and MITF-M mRNA expression.

The Effects of Salting Levels and Drying Periods on Weight Loss and Nutritional Compositions of Dry-cured Ham under Controlled Ripening Condition (건염햄 제조시 소금수준과 건조기간이 제품 중량감소 및 영양적 특성에 미치는 영향)

  • Seong, Pil-Nam;Cho, Soo-Hyun;Kang, Geun-Ho;Kim, Jin-Hyoung;Park, Beom-Young;Jeong, Da-Woon;Jung, Jae-Hong;Jeong, Seok-Geun;Kim, Dong-Hoon
    • Journal of Animal Science and Technology
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    • v.54 no.1
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    • pp.35-41
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    • 2012
  • The aim of this study was to offer information about appropriate processing method for dry cured-ham with controlled ripening condition. In this study, three different treatments were performed: High salt group (HS), 18 hams were salted with 70 g $kg^{-1}$ salt (w/w) Middle salt group (MS), 18 hams were salted with 50 g $kg^{-1}$ salt Low salt group (LS), 18 hams were salted with 30 g $kg^{-1}$ salt. Also three drying periods were applied (180 days, 270 days and 360 days). The weight loss in HS was higher (5.62%) on curing step and in LS was higher (12.35%) on post-salting step compared to other groups. On fermentation stage, weight loss of HS was higher than that of LS (p<0.05). Weight loss on drying was increased as the drying period passes (p<0.05). Moisture contents were significantly (p<0.05) decreased and fat contents were significantly (p<0.05) increased in all treatment groups as drying period increased. The different drying periods affected fatty acid compositions on all salting levels; saturated fatty acid contents were increased (p<0.05) with more drying, whereas unsaturated fatty acid contents were decreased (p<0.05) as drying period increased.

A Study on the Contents of Selenium and Zine in Human Milk (모유의 셀레늄과 아연 함량에 관한 연구)

  • 양혜란
    • Journal of Nutrition and Health
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    • v.28 no.9
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    • pp.872-879
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    • 1995
  • The changes of selenium and zine contents of transitional milk and mature milk at 7 and 60 days postpartum were comparatively studied on 20 Korean lactating women. the selenium and zinc contents of transitional milk and mature milk were analyzed by atomic absorption spectrophotometer after wet-digestion. The mean selenium contents wer 12.2$\mu\textrm{g}$ /ι and 11.5$\mu\textrm{g}$/ι at 7 and 60 days postpartum, respectively. The mean zinc contents decreased significantly from 4.23mg/ι at 7 days to 1.62mg/ι at 60 days postpartum (p<0.05).

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Physicochemical Characteristics and Antioxidant Activities of Mulberries by Greenhouse and Open Field Cultivation in Maturation Degrees (시설 및 노지 재배한 오디의 숙기별 이화학적 품질 및 항산화 활성)

  • Lee, Ji Young;Hwang, In Guk;Park, Bo Ram;Han, Hye Min;Yoo, Seon Mi;Han, Gui Jung;Park, Jong Tae;Kim, Ha Yun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.10
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    • pp.1476-1483
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    • 2015
  • This study was conducted in order to compare changes in the quality characteristics and antioxidant activity of mulberries depending on the degree of ripeness between greenhouse (GH) and open field (OF) cultivation. The mulberries were divided into five degrees of ripeness. Quality characteristics such as pH, acidity, antioxidant activity, and contents of free sugar, organic acid, polyphenols, flavonoids, and anthocyanins were investigated. pH level increased slightly while acidity decreased depending on the degree of ripeness. Fructose and glucose were detected in mulberries as the major free sugar. Fructose and glucose increased rapidly during ripening. Citric acid was major acid and decreased considerably during ripening. Contents of total polyphenols, flavonoids, and anthocyanins increased considerably in the last stage of ripening. Contents of polyphenols, and flavonoids in mulberry from OF were higher than from GH. DPPH and ABTS radical scavenging activities increased rapidly in mulberries of the last degree. Contents of functional components were not greatly different between GH and OF, whereas content of free sugar in GH was higher than that in OF.

Quality characteristics of the enhanced beef using winter mushroom juice

  • Choi, Yun-Sang;Jo, Kyung;Lee, Seonmin;Yong, Hae In;Jung, Samooel
    • Journal of Animal Science and Technology
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    • v.62 no.3
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    • pp.396-408
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    • 2020
  • This study investigated the quality properties of enhanced beef, manufactured by injecting the beef with a brine containing winter mushroom juice powder (WMJP). The enhanced beef was manufactured by injecting the eye of round with brine (15% by green weight). Four treatments consisted of control (no injection of brine) and three enhanced beef, EBS (brine containing 5 g sodium chloride per kg beef), EBW 0.2 (brine containing 5 g sodium chloride and 2 g WMJP per kg beef), and EBW 0.5 (brine containing 5 g sodium chloride and 5 g WMJP per kg beef), were tested. The effect of enhancement or WMJP on the quality properties of beef was evaluated during storage at 4℃ for 1, 5, and 10 days. Total aerobic bacteria counts between the control and the enhanced beef, and among EBS, EBW 0.2, and EBW 0.5 were not significantly different after any storage period (p > 0.05). The pH of beef was not different between the control and the enhanced beef, and among enhanced beef at 1 and 5 days of storage (p > 0.05). However, it was higher in the enhanced beef than control, and EBW 0.2 and EBW 0.5 had higher pH than EBS after 10 days of storage (p < 0.05). The enhanced beef showed a high total loss at all storage days (p < 0.05). There were no differences in total loss among enhanced beef after any storage period (p > 0.05). The enhanced beef had no consistent differences in L*, a*, and b* values with control during storage, however, EBW 0.5 showed high color stability. The hardness of the enhanced beef was significantly lower than that of the control after 10 days of storage, although the values were lower at all storage stages. EBS 0.5 had the lowest thiobarbituric acid reactive substance (TBARS) value among cooked beef of all treatments at all storage days. The enhanced beef received higher scores in all sensory properties than control, and no negative effect of WMJP was found in the sensory quality of the enhanced beef. The use of winter mushroom juice can result in quality improvement in enhanced beef.

Studies of Magnetic Properties of Ni-Zn-Cu Ferrite with Low Loss and High Permeability (저손질, 고투자율을 갖는 Ni-Zn-Cu ferrite의 자기적 특성 연구)

  • 김용복;고재귀
    • Journal of the Korean Magnetics Society
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    • v.8 no.2
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    • pp.62-66
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    • 1998
  • We have studied on the magnetic properties of the specimen with additives Bi$_2$O$_3$and$V_2O_5$ that sintered at 900 $^{\circ}C$ for 4 hours for sybthesizing optimal Ni-Zn-Cu ferrite. Curie temperature rises from 240 $^{\circ}C$ to 270 $^{\circ}C$ as Ni contents increase. Magentic maximum induction$(B_m)$ increases from 2650 G to 3300 G, 3500 G in the specimens with $V_2O_5$ and Bi$_2$O$_3$resectively. On the contrary coercive force $(H_c)$ lowers to 2.05 Oe~1.05 Oe. Permeability all increase in the specimen with additives. In the specimen with additive Bi$_2$O$_3$, we have obtained the low relative loss factor of $6.3{\times}10^{-5}~7.84{\times}10^{-5}$ in the range of 1MHz due to increase of resistivity in grain boundary. In the specimen with additive $V_2O_5$ in spite of increase permeability relative loss factor increase of due to decrease of Q-value.

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The Study on Automatic Speech Recognizer Utilizing Mobile Platform on Korean EFL Learners' Pronunciation Development (자동음성인식 기술을 이용한 모바일 기반 발음 교수법과 영어 학습자의 발음 향상에 관한 연구)

  • Park, A Young
    • Journal of Digital Contents Society
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    • v.18 no.6
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    • pp.1101-1107
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    • 2017
  • This study explored the effect of ASR-based pronunciation instruction, using a mobile platform, on EFL learners' pronunciation development. Particularly, this quasi-experimental study focused on whether using mobile ASR, which provides voice-to-text feedback, can enhance the perception and production of target English consonants minimal pairs (V-B, R-L, and G-Z) of Korean EFL learners. Three intact classes of 117 Korean university students were assigned to three groups: a) ASR Group: ASR-based pronunciation instruction providing textual feedback by the mobile ASR; b) Conventional Group: conventional face-to-face pronunciation instruction providing individual oral feedback by the instructor; and the c) Hybrid Group: ASR-based pronunciation instruction plus conventional pronunciation instruction. The ANCOVA results showed that the adjusted mean score for pronunciation production post-test on the Hybrid instruction group (M=82.71, SD =3.3) was significantly higher than the Conventional group (M=62.6, SD =4.05) (p<.05).

Water Requirement of Maize According to Growth Stage (노지재배 옥수수의 생육시기별 물 요구량 구명)

  • Eom, Ki-Cheol;Park, So-Hyun;Yoo, Sung-Yung
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.1
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    • pp.16-22
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    • 2013
  • Water is the most important resource for the maximum water use efficiency and yield of maize. Water has to be applied moderately based on the water requirement of maize. Crop water requirement (WR) is a function of the potential evapo-transpiration (PET) and crop coefficient (Kc). PET can be estimated by the climate data measured at the weather station in the production region. Kc was measured by the NIAST (RDA) through lysimeter experiments. In this study, the growth stage of maize was divided into five ones (G-1: Apr. 25 ~ May 20, G-2: May 21 ~ Jun. 20, G-3: Jun. 21 ~ Jul. 20, G4: Jul. 11 ~ Jul. 25, G5: Jul. 26 ~ Aug. 20). The average PET during maize growing season of the 45 areas was 2.85 mm $day^{-1}$. The highest water requirement was at the G-3 stage among the maize growth stages. The mean water requirement (MWR) according to growth stage was 1.74 ~ 2.42 (average 2.02), 2.99 ~ 4.21 (average 3.41), 3.82 ~ 5.25 (average 4.41), 3.05 ~ 4.31 (average 3.48), and 2.62 ~ 3.49 (average 3.01) mm $day^{-1}$ in the G-1, G-2, G-3, G-4 and G-5 stage, respectively. The total water requirement (TWR) according to growth stage was 45.37 ~ 63.04 (average 52.56), 92.54 ~ 130.59 (average 105.77), 76.46 ~ 105.09 (average 88.14), 45.73 ~ 64.67 (average 52.20), and 68.25 ~ 90.75 (average 78.33) mm in the G-1, G-2, G-3, G-4 and G-5 stage, respectively.

A Study on pH and Soluble Reactive Phosphorus (SRP) from Litter Using Various Poultry Litter Amendments During Short-Term: A Laboratory Experiment (다양한 깔짚 첨가제 이용이 단 기간 깔짚내 pH와 수용성 인에 미치는 영향에 관한 연구)

  • Choi, In-Hag;Yi, Seong-Joon;Kim, Chang-Mann
    • Journal of Environmental Science International
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    • v.17 no.2
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    • pp.233-237
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    • 2008
  • The objective of this study was conducted to evaluate the effects of poultry litter amendments on pH and soluble reactive phosphorus (SRP) in poultry litter. Two laboratory studies were conducted for 42 d in Exp. 1 and for 10 d in Exp. 2, respectively. The poultry litter was treated with various amendments which included 4 g fly ash and 4 g $AlCl_3\;(AlCl_36H_2O)/100g$ litter in Exp. 1 and 4 g alum$(Al_2(SO_4){_3}\;14H_2O)$, 8 g alum, 8.66 g liquid alum, and 17.3 g liquid alum/100 g litter in Exp. 2; untreated litter served as controls. There were no differences in pH between control and T1(4 g fly ash) and SRP contents between T1(4 g fly ash) and T2(4 g $AlCl_3$) in Exp. 1. A significant difference in pH and SRP contents in Exp. 2 was observed among all treatments(P< 0.05). In experiment 1, T1(4 g fly ash) and T2(4 g $AlCl_3$) at 42 d decreased SRP in litter by 47.1% and 62.6% of that from litter alone, respectively. In experiment 2, T1(4 g alum), T2(8.66 g liquid alum), T3(8 g alum), and T4(17.3 g liquid alum) treatments at 10 days reduced SRP contents by up to 36.2%, 62.9%, 87.0%, and 83.9%, respectively, when compared with the controls. Decrease in SRP contents was chiefly associated with reduction in litter pH. These results indicate that use of various litter amendments to limit P solubility has potential and should be pursued as a means of reducing soluble reative phosphorus during short term.

Lesser known indigenous vegetables as potential natural egg colourant in laying chickens

  • Abiodun, Bolu Steven;Adedeji, Aderibigbe Simeon;Abiodun, Elegbeleye
    • Journal of Animal Science and Technology
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    • v.56 no.5
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    • pp.18.1-18.5
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    • 2014
  • Background: A six-week study involving two hundred and fifty (250) Harco Black layer birds at point of lay was conducted to investigate the effects of potential natural colorant on performance and Egg quality traits. The birds were assigned to five (5) dietary treatments, each containing supplements either of control, Baobab Leaf (BL), Waterleaf (WL), Red Pepper (RP), Canthaxanthin (CTX) at 40 g/kg feed and 50 mg/kg feed of natural and commercial colorants, respectively. Results: Performance records shows that there was no significant (p > 0.05) difference in feed intake across the supplements of Red pepper, Water leaf, Canthaxanthin and control diet, however, birds fed Baobab leaf treatment had a significantly lower (p < 0.05) feed intake value (94.07 g) when compared with other treatments. Body weight gain and Hen Day Production were not significant influenced (p > 0.05) by the dietary treatments, although laying hens fed Baobab leaf supplement had lowest mean HDP of 48.80%, while birds fed Red pepper and Water leaf supplement had an average value of 52.79%. There was no significant effect (p > 0.05) of colorants on egg external traits, compared with the control; birds fed Canthaxanthin treatment had higher mean egg weight (51.79 g), egg length (4.55 g), egg breadth (3.29 g); Red pepper treatment had highest mean shell thickness (0.29 g), however these differences were not significant (p > 0.05). Yolk height, Albumen height, Yolk index, and Haugh unit were not significantly affected (p > 0.05) across treatments. Yolk width was lowest (p < 0.05) in Baobab leaf treatment (2.54 cm); Red pepper, Water leaf and Canthaxanthin (2.89 cm, 2.62 cm and 2.89 cm respectively) were not significantly (p > 0.05) different from the control (2.73 cm). Yolk colour score was significantly highest (p < 0.05) in Red pepper treatment (7.50); Water leaf, Baobab leaf and Canthaxanthin ranged between 2.25-3.31 on the DSM yolk colour fan, Control treatment had the lowest yolk colour score (p < 0.05) of 1.31. Conclusion: The study showed Red pepper as a worthy alternative to commercial yolk colorant. Water leaf and baobab are not good substitutes for canthaxanthin as a yolk colourant.