• Radiation Oncology Journal
• /
• v.18 no.3
• /
• pp.200-204
• /
• 2000

Purpose : We already reported the results that aqueous extract of Korean ginseng roots showed a marked cytotoxicity. In this study, we investigated whether combined ginseng product with X-irradiation increase the cytotoxicity of tumor cells than X-irradiation or not. Materials and Methods : Fifty gram of Korean ginseng powder mixed with 1 L of distilled water was extracted with reflux flask under condition of $100^{\circ}C$ for 5 hrs. This aquaous ginseng extract was filtered, centrifuged and then was freezed under condition of $-90^{\circ}C$ for 16-18 hrs. The freezing extract was dried with freeze drier, and then diluted. X-irradiation was given to tumor cells by 6 MeV linear accelerator. The cytotoxicity of ginseng in vitro was evaluated from its ability to reduce the clonogenecity of fibrosarcoma (FSa II) cells. In X-irradiation alone group, each 2, 4, 6 and 8 Gy was given to tumor cells. In X-irradiation with ginseng group, 0.2 mg/mL of ginseng extract was exposed to tumor cells for 1 hour before X-irradiation. Results : The yield for 50 g of ginseng extract which was treated with freezing drier was 3.13 g($6.3\%$). Cytotoxicity In vitro was measured as survival fraction which was judged from the curve, at ginseng concentration of 0.001, 0.01, 0.1 and 1 mg/mL were $0.89\pm0.04$, $0.86\pm0.06$, $0.73\pm0.01$ and $0.09\pm0.02$, respectively. Survival fraction at X-irradiation alone of 2, 4, 6 and 8 Gy were $0.81\pm0.07$, $0.42\pm0.08$, $0.15\pm0.02$, $0.03\pm0.01$, respectively. But, suwival fraction in combined group of X-irradiation and ginseng (0.2mg/ml) at each same radiation dose were $0.28\pm0.01$, $0.18\pm0.03$, $0.08\pm0.02$, $0.006\pm0.002$, respectively (p<0.05). Conclusion : The yield for ginseng extract which was treated with freezing drier was $6.3\%$. Cytotoxicty of Fsa 11 in combined ginseng with X-irradiation group was increased than that of X-irradition alone group, and its enhancing effect seemed to be added.

• Journal of fish pathology
• /
• v.4 no.1
• /
• pp.1-11
• /
• 1991

Histopathological changes and methemoglobinemia of the eels (Anguilla japonica) reared at six culture farms in the vicinity of Y$\check{o}$su city were investigated under the conditions of $22.0{\sim}29^{\circ}C$, 4.33~7.33ppm of D.O., pH 6.1~8.2 and 0.03~10.1ppm of nitrite from December, 1989 to June, 1990. All of the eels showed no any abnormal state in action, color and blood, but the eels reared in pH 6.0 had planty of mucus and stickiness on their body surface. Methemoglobin levels were 1.03~9.86% of total erythrocyes in Dec. and 5.05~25.69% in June, and poikilocyte levels were 5.8~30.17% in Dec. and 8.67~74.45% in June. These results indicate the fact that the water management of the culture farms was worse in summer than in winter, even though not good in winter. Hypertrophy was found in the gill, hepatic and kidney tissue of the eels in all the culture farms. In March and June, Fish of all culture farms appeared the detachment of gill lamella epithelium cells, pyknosis and sinusoids of nucleus in the hepatic tissue and hemosiderosis in the kidney tissue. The trend of increase in methemoglobin levels appeared in the fish of culture farms with high nitrite concentration, which didn't appear equally in all the culture farms. However, in the culture farms of pH 6.0, methemoglobin and poikilocyte levels were increased independently of concentration of nitrite, and degeneration in the tissue of the gill, liver and kidney was also appeared.

• Journal of Korean Academy of Nursing
• /
• v.27 no.2
• /
• pp.364-376
• /
• 1997

This study was made to analyse the nursing professional attitudes and mental health of clinical nurses in order to promote good mental health and positive attitudes. The 150 subjects obtained from 3 hospitals in Seoul completed a self-report questionnaire. Demographic data was collected in addition to data about nursing professional attitudes and mental health. The data was analyzed by SAS, t-test, scheffe's test, pearson's correlation and the GLM procedures. The results of the data analysis as follows : 1) Nursing professional attitudes were positive (MS=3.76) 2) Mental health was shown to be satisfactory (MS=0.65) 3) The relationship between nursing professional attitudes and mental health of the subjects were shown to be significant according to the professional education level with depression(r=-0.19, p=0.03/sup */), anxiety(r=-0.23, p=0.01/sup **/), hostility(r= -0.19, p=0.03/sup */), phobicanxiety(r=-0.18, p=0.04/sup */), but there was a significant difference according to the pursit of social profit with anxiety(r=-0.17, p=0.03/sup */). There was a significant difference according to autonomy with somatization(r=-0.23, p=0.01/sup **/), obsessive-compulsive(r=-0.22, p=0.01/sup **/), depression(r=-0.20, p=0.02/sup */), anxiety(r=-0.17, p=0.05/sup */). Finally, There was a significant difference according to ethics with hostility(r=-0.17, p=0.05/sup */). 4) According to nursing professional attitudes and demographic fators there was a significant difference in the professional position(t=2.01, p=0.05/sup */), work location(f=4.99, p=0.01/sup **/), length of employment (f=3.66, p=0.03/sup **/). 5) The nursing professional attitudes and occupational factors showed a positive correlation with the anticipation of length of employment (t=2.00, p=0.05/sup */). 6) According to Mental health and dmographic factors there was a significant correlation with the professional position(t=-2.21, p=0.03/sup */). 7) According to mental health and occupational factors there was a significant difference correlation. Based on the study results, the following recommendation are made : 1) Nursing education curriculum should be developed for the continuous promotion of mental of professional nurses. 2) Follow up study of identification of the variables which positively influence professional nurses attitudes.

• Journal of Aquaculture
• /
• v.16 no.3
• /
• pp.159-164
• /
• 2003

Effect of the benthic echiurid Urechis unicinctus juveniles on the chemical characteristics of organically enriched sediment, as well as the individual survival rate and growth in various sediments, was investigated in this study to ascertain the effectiveness of U. unicinctus in the bioremediation of organically polluted sediment. The content of acid volatile sulfide (AVS) in the sediment of three coastal areas was 0.4±0.1 mg/gㆍdry sediment in Seonso, 0.1±0.01 mg/gㆍdry sediment in Myo-do, 0.02±0.01 mg/gㆍdry sediment in Dolsan-do, respectively. 500 juveniles with average body length of 1.2±0.3 mm were bred in these sediments. After 30 days, the AVS content of three places decreased to 0.29±0.3, 0.08±0.01 and 0.0l±0.01 mg/gㆍdry. After 60 days, the content was 0.23±0.03, 0.0l±0.01, 0.0l±0.01 mg/gㆍdry. The AVS content in the control (sediment from Seonso without juveniles) was 0.39±0.75 mg/gㆍdry sediment (after 30 days) and 0.45±0.01 mg/gㆍdry sediment (after 60 days). At the same time, the chemical oxygen demand (COD) and total ingition loss (TIL) of these sediments showed the same trend with that of AVS. Furthermore, 60 days later, the survival rate and growth of juveniles were 71.2%, 11.1±l.0 mm in Seonso, 89.8%, 12.0±1.3 mm in Myo-do and 81.6%, 11.9±1.9 mm in Dolsan-do. It was suggested that U. unicinctus can survive better in less polluted environment. Under different U. unicinctus juvenile density (100, 300, 500, 700, 900 individuals), after 60 days cultivation, the AVS content decreased from 0.4±0.1, 0.4±0.1, 0.4±0.3, 0.4±0.3, 0.4±0.3 mg/gㆍdry to 0.4±0.2, 0.3±0.2, 0.2±0.1, 0.2±0.1, 0.2±0.1 mg/gㆍdry. Therefore, the higher the density of juveniles, the greater the effect in improving the sediment. In comparison to different initial AVS concentration (0.40±0.01, 0.35±0.01, [I can't find a reference to this number above], 0.29±0.02, [I can't find a reference to this number above], 0.18±0.03, [I can't find a reference to this number above], 0.06±0.02 mg/gㆍdry sediment [I can't find a reference to this number above] and the control 0.0±0.0[I can't find a reference to this number above]), after 30 days cultivation with 500 juveniles, the AVS content was 0.29±0.03, 0.19±0.0, 0.15±0.01, 0.12±0.02, 0.05±0.0mg/gㆍdry and 0(control). After 60 days cultivation, the content was 0.23±0.03, 0.12±0.4, 0.10±0.01, 0.18±0.03, 0.06±0.02 mg/gㆍdry and 0 (control). Use % decrease in AVS content to state: The impact of U. unicinctus on the sediments was greater with higher concentrations of AVS. The results of this study indicate that U. unicinctus is effective in the biological treatment of sediments polluted by AVS and other organics.

• Korean Journal of Human Ecology
• /
• v.8 no.1
• /
• pp.33-48
• /
• 2005

The purpose of this is to agar powder addition to retard retragradation for making Backsulgi. Backsulgi is manufactured promptly by adding ingredients having agar powder and then observation of sensory and texture characteristics. Then Backsulgi determinded in storing at the temperatures of 20 and Celsius degrees for 0, 24, 48 and 72 hours, respectively. The results from this study are as follow; 1. Moisture absorption of control show 34.4%, compared with, Backsulgi with agar powder added 3%, 6%, 9%, 12%, 15% show higher 37.03%, 39.28%, 41.72%, 43.5%, 44.70%, respectively. 2. In terms of chromaticity, lightness of control show $70.68{\pm}7.248{\sim}61.25{\pm}0.90$, the longer time is and much agar powder Backsulgi show lower and 15% agar powder added Backsulgi show $51.53{\pm}0.921{\sim}54.715$. Then redness(a) of control show $-0.70{\pm}0.14$, compared with 15% agar powder added Backsulgi show $-0.32{\pm}0.047$. 15% agar powder Backsulgi show $9.03{\pm}0.313$ higher in yellowness than control $5.47{\pm}0.40$. 3. Control and agar powder Backsulgi was shown pH 6.1~6.3, the longer time is, all Backsulgi was shown pH 5.5~5.9 and much agar powder Backsulgi was shown an weakly acidity. 4. Swelling and pore ratio of control was shown 85.36% and 118.32%, compared with, agar powder added Backsulgi was higher a rate of increase. 5. For a sensory evaluation, the longer time is, adding agar powder rice cake is more perfected at 20$^{\circ}C$ in times if smooth, inner moisture, chewiness, after selling, overall quality. 6. Determination of texture characteristics by a reometer shows that Backsulgi with agar powder added change more in hardness that controls, indicating that effect of retarding retrogadation is higher when storage time is longer. 7. The correlation of hardness, springness between sensory and instrumental evaluation showed significantly positive function.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.103-103
• /
• 2003

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were $-14.8 \pm 33.9%$ (P<0.05) of the number before transplantation, however, the ratio increased slightly to $13.6 \pm 56.3%$ in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

• Korean Journal of Food Science and Technology
• /
• v.23 no.4
• /
• pp.400-404
• /
• 1991

Protease was purified from Aspergillus niger propagated on katsuobushi. The optimal pH and temperature of the enzyme were 7.2 and $45^{\circ}C$, respectively. The enzyme was stable at $pH\;5{\sim}8$ and at below $40^{\circ}C$. Enzyme activity was promoted by $K^{-}\;and\;Fe^{2+}$, whereas it was inhibited by $Hg^{2},\;Zn^{2},\;Mn^{2}\;and\;Cd^{2}$. The acidic, basic and neutral amino acid compositions were found to be 22.63, 13.57 and 63.80%, respectively. The content of nonpolar, poler and sulfur-containing amino acids were 39.72, 20.03 and 9.53% respectively, and aspartic and glutamic acids were abundant. The molecular weight was 42,000 and isoelectric point was estimated pH 5.6.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.34 no.12
• /
• pp.9-17
• /
• 2006

An experimental study was carried out to examine the behavior of the unsteady boundary layer. An NACA 0012 airfoil with aspect ratio of 2.7 was set vertically in a test section, which is sinusoidally pitched about the quarter chord. The oscillating amplitude is from -6$^{\circ}$ to +6$^{\circ}$ and the mean angle of attack is 0$^{\circ}$. Surface mounted probes (Glue-on probes) were employed to measure the surface flow of the boundary layer. Measurements were made at free-stream velocities of 1.98, 2.83, and 4.03m/s, and the corresponding Reynolds numbers based on the chord length were 2.3$\times$104, 3.3$\times$104 and 4.8$\times$104, respectively. The reduced frequency is fixed as 0.1 in all cases. The results show that the surface position of minimum shear stress and of boundary layer break-down can be discerned in the Reynolds number between 2.3$\times$104 and 3.3$\times$104.

• Journal of fish pathology
• /
• v.10 no.2
• /
• pp.177-186
• /
• 1997

To establish effectively for isolation and cultivation and cultivation of scuticociliata, we tried to culture scuticociliata using culture medium(P2Y1-DW medium). The number of scuticociliata reached $1.50{\times}10^3$ cells/ml at the temperature of $25^{\circ}C$ in 3 days, but $1.03{\times}10^2$ cells/ml at $15^{\circ}C$. In P1Y1-S medium growth reached $2.6{\times}10^4$cells/ml in 3 days, but the number in DW medium was the lowest with $2.0{\times}10^2$cells/ml. In all these media, the number decreased after 5 days. As the method of the parasite`s division, after attaching the anterior part of the body, its shape gradualy changed to a western pear-like appearance. The parasite completely divided into two cells, then freely swam and became an adult cell, size of $40{\mu}m$ in size within about 3 hours from attaching.

• Journal of Nutrition and Health
• /
• v.43 no.6
• /
• pp.628-637
• /
• 2010

To determine vitamin A and E intakes and their food sources, dietary intakes were collected by three consecutive 24-hour recalls from 192 adults living in Seoul and Gyeonggi Province, Korea. The mean vitamin A, retinol and ${\beta}$-carotene intakes were $1240.1{\pm}1101.1\;{\mu}g$ retinol equivalent/day ($693.3{\pm}563.2\;{\mu}g$ retinol activity equivalent/day), $182.6{\pm}149.5\;{\mu}g$/day and $5443.3{\pm}6365.5\;{\mu}g$/day, respectively. Only 9.4% of the subjects consumed less than the Korean Estimated Average Requirement for vitamin A. The mean vitamin E intake was $6.03{\pm}2.54\;mg$ ${\alpha}$-tocopherol equivalent/day. The ${\alpha}$-tocopherol and ${\gamma}$-tocopherol intakes were $4.83{\pm}2.03$ and $5.57{\pm}3.41\;mg$/day, respectively. Most of the subjects (93.8%) consumed less than the Korean Adequate Intake for vitamin E. The major food sources of vitamin A were sweet potato, carrot, red pepper powder, spinach, and citrus fruit, and the top 30 foods provided 91.5% of total Plant foods provided 81.0% and animalderived foods 10.5% of the vitamin A intake from the top 30 foods. The major food sources of vitamin E were soybean oil, red pepper powder, Ramyeon (cup noodles), spinach, and egg. The top 30 foods provided 78.0% of total vitamin E intake. Plant foods provided 61.3% and animal-derived foods 15.9% of the vitamin E intake from the top 30 foods. In conclusion, the vitamin A intake of the Korean adults in this study was ge-nerally adequate, but the vitamin E intake of many subjects was inadequate. Therefore, nutritional education may be of benefit to Korean adults to increase their vitamin E intake.