• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.321-324
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• 2002

In situ recovery of recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) was performed in transgenic Nicotiana tabacum cell suspension cultures. Aqueous two-phase systems (ATPS) were used to utilize its biocompatibility. Transgenic plant cells could be grown up to 15.7 g/L in normal medium and 18.6 g/L in ATPS composed of 6% (w/w) polyethylene glycol (PEG) 20,000 and 10% (w/w) dextran 2,000,000. It was proved that the A TPS was not harmful to cell growth. In addition, It is expected to recover hGM-CSF simultaneously with cell growth. Using this method, maximum hGM-CSF concentration at 1.64 ng/mL was obtained on day 3.

• Journal of Microbiology and Biotechnology
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• v.7 no.1
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• pp.62-67
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• 1997

To enhance the hyperproductive and low energy-consuming ethanol fermentation rate, the thermotolerant yeast S. cerevisiae RA-74-2 cells were immobilized. An efficient immobilization condition was proved to be $1.5{\%}$ (w/v) alginate solution, neutral pH and 20 h activation of beads. The fermentation characteristics and stability at various temperatures were examined as compared with free S. cerevisiae RA-74-2 cells. The immobilized cells had excellent fermentation rate at the range of pH 3-7 at 30-$42^{\circ}C$ in 15-$20{\%}$ glucose media. When the seed volume was adjusted to 0.12 (v/v) (6ml bead/50 ml medium), $11{\%}$ (w/v) ethanol was produced during the first 34 hand $12.15{\%}$ (w/v) ethanol [$95{\%}$ (w/v) of theoretical yield] during the first 60 h in $25{\%}$ glucose medium. In repetitive fermentation using a 2 litre fermentor, 5.79-$7.27{\%}$ (w/v) ethanol [76-$95{\%}$ (w/v) of theoretical yield] was produced during the 40-55 h in $15{\%}$ glucose media. These data suggested the fact that alginate beads of thermotolerant S. cerevisiae RA-74-2 cells would contribute to economic and hyperproductive ethanol fermentation at high temperature.

• Korean Journal of Poultry Science
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• v.4 no.1
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• pp.66-74
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• 1977

닭의 혈액상에 관한 발표는 많으나 아직 우리나라에서의 보고는 거의 없다. 이에 산난능력이 우수한 닭과 병계에 대한 앞으로의 혈액학적 연구를 위한 기초적자료를 얻고저 임상적으로 건강한 소위 한국 재내종(LCH)과 백색레그혼 (WLH)암탉의 정상 혈액학치 즉 적혈구, 백혈구, 혈색소, 헤마토크리프. 평균적혈구 용적, 평균 적혈구 혈색소량. 평균 적혈구 혈색소 농도 등을 조사하였다. 1 R.B.C.는 L.C.H.에서 3.18$\pm$0.10million per c$\mu$. mm였으며 W.L.H.는 3.23$\pm$0.05였다. 2. W.B.C.는 L.C.H에서 42.17$\pm$3.35, thousand per. c$\mu$mm였으며 W.L.H.는 35.64$\pm$2.89였다. 3. Hemoglobin치는 L.C.H.에서 11.24$\pm$0.30gm per 100$m\ell$였으며 WL.H.는 38.40$\pm$0.75였다. 4. Hematocrit치는 L.C.H.에서 34.00$\pm$0,98 percent였으며, W.L.H.는 33.40$\pm$0.75였다. 5. M.C.V.는 L.C.H에서 103.66$\pm$1.22 c$\mu$ 였으며, W.L.H.는 103.32$\pm$1.28이였다. 6. M.C.H,는 C.L.H.에서 34.19$\pm$0.41 $\mu$ $\mu\textrm{g}$9였으며. W.L.H,는 34.37$\pm$0.31이었다. 7. M.C.H.C는 L.C.H.에서 33.09$\pm$0.22 percent였으며, W.L.H.는 33.25$\pm$0.36 0.36이었다. (중략)

• KSBB Journal
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• v.19 no.6 s.89
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• pp.478-483
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• 2004

The optimum fermentation conditions of Propionibacterium acidipropionici were determined for the maximum production of organic acids. Three strains of P. acidipropionici, ATCC 25562, 4875, 4965 were selected to test the productivity of organic acids in the batch fermentation. Nutrients and environmental conditions on cell growth were defined by series of experiments, and the optimum amounts of peptone, yeast extract were determined to be $1.5\%$ (w/v), $0.75\%$ (w/v), respectively. The yields and productivity were highest at pH 6 among the ranges of $5.0{\sim}7.5$, and ATCC 4965 was determined to be the best strain compared to the others by getting total productivity of 0.29 g total acids/L/h. Approximately $1.60{\sim}2.34$ moles of propionic acid and $0.74{\sim}1.05$ moles of acetic acid were produced from 1.5 moles of glucose.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.4
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• pp.633-641
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• 1995

The effect of the gap width on conjugate heat transfer in the cylindrical annulus for an insulated tube has been studied numerically by the finite difference method. The parameters considered here are the Rayleigh number, Ra, the dimensionless insulated wall thickness, $W/D_i$ and the dimensionless gap width, S/W. As S/W increases, the mean wall temperature increases at the inside wall of annulus and decreases at the outside walls of annulus and the insulated tube at $S/W{\leq}0.5$, and then slightly increases at $Re=10^4$, $W/D_i=1.47$. The heat transfer rate decreases at $S/W{\leq}0.5$ and then increases apparently as S/W increases at $Re=10^4$, W/Df=1.47. Therefore, it is considered that $$S/W{\sim_=}0.5$$ is the optimum gap width for the effect of insulation at $Re=10^4$, $W/D_f=1.47$.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.34 no.3
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• pp.302-308
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• 1998

The author examined the response of Yoroi-mebaru, Sebastes hubbsi [MATSUBARA] to the underwater attracting lamps (0.5 W, 0.8 W, 1 W) line in the experimental water tank (550 L x 58 W x 73 H cm). The attracting rate investigated in accordance with the intervals of lighting and putting out hour (1, 5 minutes) when each of the underwater attracting lamps was gradually switched off after they were switched on all at once. The results are a follows: 1. Distribution rates of fish in the illuminated area was 35.7 % in case of 1 minute interval, and 50.3 % in case of 5 minutes interval. 2. Mean distribution rate of fish at the illuminated section: \circled1 Distribution rates at interval of 1 minute were 9.8 % in 1 W, 6.5 % in 0.8 W and 5.1 % in 0.5 W, respectively. \circled2 Distribution rates at interval of 5 minutes were 15.6 % in 1 W, 8.5 % in 0.5 W and 6.1 % in 0.8 W, respectively. 3. Attracting rates of the last section showed a little increasing as illuminating time elapsed. A difference of attracting rates according to lighting source in 5 minutes interval were bigger than that in 1 minute interval. 4. Attracting rates of fish in only last section switched on: \circled1 Attracting rates at interval in case of 1 minute were 30.0 % in 0.8 W, 16.0 % in 1 W and 8.0 % in 0.5 W, respectively. \circled2 Attracting rates at interval in case of 5 minutes were 56.0 % in 1 W, 30.7 % in 0.5 W and 10.7 % in 0.8 W. respectively.

• Korean Journal of Crystallography
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• v.6 no.2
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• pp.93-97
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• 1995

Re(≡NC6H5)(PPh3)2CI3, I, reacted with, 1,2-bis(diphenylphosphino)ethane (DPPE) to give fac-Re(≡NC6H5)(DPPE)CI3, II. The product has been characterized by 1H-NMR, elemental analysis, and X-ray crystallography. II Crystalizes in the monoclinic space group Pc, with cell parameters a=11.083(3)Å, b=10.930(1)Å, c=14.081(2)Å, β=108.37(2)°, Z=2. Least-squares refinement of the structure led to a R(wR2)factor of 0.0254(0.0607) for 2888 unique reflections of I>2σ(I) and for 352 variables.

• Applied Chemistry for Engineering
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• v.8 no.4
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• pp.582-588
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• 1997

Synthetic reaction of TAME, ETBE, and MTBE compounds used largely for gasoline octane number enhancer to prevent air pollution was investigated using heteropolyacid catalyst in a fixed bed flow reactor. In the synthetic reaction of TAME, ETBE and MTBE, after hetero atom being replaced with poly atom, the activity of the catalyst, $H_4SiW_{12}O_{40}$ with coordinated bond with W and an hetero atom of Si was the highest among the catalysts tested. Also the activity depended upon the metals replaced which are related to the catalyst acidity. $FeHPW_{12}O_{40}$ and $K_3PM_{o12}O_{40}$ catalysts showed high activity in TAME synthesis, while they were not effective in ETBE and MTBE synthesis. In this study catalysts showing high activity were selected and mixed with equal weight combination of $H_4SiW_{12}O_{40}$ and $Sr_2SiW_{12}O_{40}$ ;$H_4SiW_{12}O_{40}$ and $NaH_2PW_{12}O_{40}$ ; $Fe_{1.5}PW_{12}O_{40}$ and $Mg_2SiW_{12}O_{40}$ ; $Mg_2SiW_{12}O_{40}$ and $Ba_2SiW_{12}O_{40}$. The mixed heteropolyacid catalysts showed higher TBA conversion rate and better selectivity of ETBE and MTBE than the single catalysts.

• Journal of Pharmaceutical Investigation
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• v.29 no.1
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• pp.1-5
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• 1999

The bioavailabilities of baicalin in water, oil, w/o and o/w emulsion were evaluated in rats. The dissolution rate of baicalin in o/w emulsion was smaller than those of w/o form in dilute hydrochloric acid solution (pH 1.2) and in PBS (pH 6.8). The absorption rate of baicalin from w/o emulsion was smaller than that of o/w emulsion in the different parts of rat intestine of the rats. Following oral administration in rats, the $C_{max}$ of baicalin from water phase, oil phase, o/w wand w/o emulsion were 2.11, 0.61, 1.57, and $1.35\;{\mu}g/ml$, respectively. The relative bioavailability of w/o emusion was 129 % when it was compared with water phase. This result suggests that the improvement of bioavailability for baicalin in w/o emulsion might be practically available.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.139-146
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• 2004

A bacterium producing alkaline cellulase was isolated from soil, leaf mold and compost, and was identified as alkalophilic Bacillus sp. HSH-810 by morphological, cultural and biochemical determination. The optimum cul-ture condition of Bacillus sp. HSH-810 for the growth and alkaline cellulase production was $30^{\circ}C$ and pH 10.0. The maximum alkaline cellulase production was obtained when 1.0%(w/v) CMC, 0.5%(w/v) peptone, 0.02%(w/v) $CaCl_2$ and 0.02(w/v) $CoCl_2$ were used as carbon source, nitrogen source and mineral source, respectively. The optimum pH and temperature of the enzyme activity were pH 10.5 and $50^{\circ}C$, respectively. This enzyme was fairly stable in the pH range of 6.0-13.0 and at $50^{\circ}C$. For the effect of surfactants, the activity of alkaline cellulase was stable in the presence of sodium-$\alpha$-olefin sulfonate (AOS), sodium dodecyl sulfonate (SDS), Tween 20 and Tween 80, but inhibited by the presence of 0.1 linear alkyl-benzene sulfonate (LAS) sig-nificantly.