• Journal of Yeungnam Medical Science
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• v.10 no.2
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• pp.432-444
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• 1993

Multidrug resistance(MDR) phenotype is frequently observed in animal and human cancer cell lines selected for in vitro resistance to a single chemotherapeutic agent. It is characterized by the diminished drug accumulation and is related to the drug efflux mechanism in resistant cells. In the present study, adriamycin resistant cells(L1210-$AdR_6$ : $10^{-6}M$ adriamycin, $-AdR_5$ : $10^{-5}M$) and vincristine resistant cells (L1210-$VcR_7$ : $10^{-7}M$ vincristine, $-VcR_6$ : $10^{-6}M$) were produced from mouse lymphoblastic leukemia cell line L1210. Growth profiles of survived cells were observed for 5 days with MTT(thiazolyl blue) assay and resistance was compared with $IC_{50}$(drug concentration of 50% survival reduction in absorbance). Resistant cells proliferated more slowly than sensitive cell. Doubling times were 29.7hr in L1210, 68.7hr in L1210-$AdR_5$ and 58.2hr in $-VcR_6$. MDRs expressed as resistance factor were as follows, L1210-$AdR_5$ was 76.4 times for vincristine, L1210-$VcR_6$ was 96.4 times for adriamycin. The cell membrane proteins with three different M.W. were recognized to be related resistance, 220, 158, and 88 kd in L1210-$AdR_5$, 158, 140 and 88 kd in L1210-$VcR_6$ by SDS-PAG electrophoresis. Cell surface membrane proteins were identified by radio-iodination and autoradiogram, their molecular weights were 158, 72.8, and 42.4 Kd in L1210-$VcR_6$.

• Animal Bioscience
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• v.36 no.6
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• pp.851-860
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• 2023

Objective: This study aims to evaluate the target genes of gga-miR-20a-5p and the regulated immune responses in the chicken macrophage cell line, HD11, by the exosome-mediated delivery of miR-20a-5p. Methods: Exosomes were purified from the chicken macrophage cell line HD11. Then, mimic gga-miR-20p or negative control miRNA were internalized into HD11 exosomes. HD11 cells were transfected with gga-miR-20a-5p or negative control miRNA containing exosomes. After 44 h of transfection, cells were incubated with or without 5 ㎍/mL poly(I:C) for 4 h. Then, expression of target genes and cytokines was evaluated by quantitative realtime polymerase chain reaction. Results: Using a luciferase reporter assay, we identified that gga-miR-20a-5p directly targeted interferon gamma receptor 2 (IFNGR2), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase kinase kinase 5 (MAP3K5), and mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Moreover, the exosome-mediated delivery of gga-miR-20a-5p successfully repressed the expression of IFNGR2, MAPK1, MAP3K5, and MAP3K14 in HD11 cells. The expressions of interferon-stimulated genes (MX dynamin like GTPase 1 [MX1], eukaryotic translation initiation factor 2A [EIF2A], and oligoadenylate synthase-like [OASL]) and proinflammatory cytokines (interferon-gamma [IFNG], interleukin-1 beta [IL1B], and tumor necrosis factor-alpha [TNFA]) were also downregulated by exosomal miR-20a-5p. In addition, the proliferation of HD11 cells was increased by exosomal miR-20a-5p. Conclusion: The exosome-mediated delivery of gga-miR-20a-5p regulated immune responses by controlling the MAPK and apoptotic signaling pathways. Furthermore, we expected that exosomal miR-20a-5p could maintain immune homeostasis against highly pathogenic avian influenza virus H5N1 infection by regulating the expression of proinflammatory cytokines and cell death.

• Proceedings of the Safety Management and Science Conference
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• 2001.05a
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• pp.127-136
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• 2001

본 연구는 ISO 9001：1994에 기초한 TL 9000 요건： R 2.5 추가요구사항과 문서화 영향에 대해 언급하고 6가지 LCM 모델을 제시한다. 끝으로 ISO 9001 : 2000에 기초한 TL 9000 요건 : R 3.0 추가요구사항을 R 2.5의 대비표로 소개한다.

• Proceedings of the Safety Management and Science Conference
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• 2001.05a
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• pp.117-126
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• 2001

본 연구는 TL 9000 성과지표 : R 2.5 및 이를 QUEST FORUM Web Site에서 등록하기 위한 절차 및 Data Flow를 소개한다. 끝으로 TL 9000 성과지표 : R 3.0 및 Product Category 중 R 2.5에서 개정된 주요부분을 소개하며 향후 성과지표 개발 가이드라인에 대해 언급한다.

• Animal Bioscience
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• v.37 no.7
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• pp.1156-1167
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• 2024

Objective: MicroRNAs (miRNAs) are endogenous non-coding RNAs that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. However, the precise functional mechanism of its regulation of fat metabolism is not fully understood. Methods: We identified bta-miR-365-3p, which specifically targets the 3' untranslated region (3'UTR) of the FK506-binding protein 5 (FKBP5), and verified its mechanisms for regulating expression and involvement in adipogenesis. Results: In this study, we found that the overexpression of bta-miR-365-3p significantly decreased the lipid accumulation and triglyceride content in the adipocytes. Compared to inhibiting bta-miR-36 5-3p group, overexpression of bta-miR-365-3p can inhibit the expression of adipocyte differentiation-related genes C/EBPα and PPARγ. The dual-luciferase reporter system further validated the targeting relationship between bta-miR-365-3p and FKBP5. FKBP5 mRNA and protein expression were detected by quantitative real-time polymerase chain reaction and Western blot. Overexpression of bta-miR-365-3p significantly down-regulated FKBP5 expression, while inhibition of bta-miR-365-3p showed the opposite, indicating that bta-miR-365-3p negatively regulates FKBP5. Adenosine 5'-monophosphate (AMP)-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway is closely related to the regulation of cell growth and is involved in the development of bovine adipocytes. In this study, overexpression of bta-miR-365-3p significantly inhibited mRNA and protein expression of AMPK, mTOR, and SREBP1 genes, while the inhibition of bta-miR-365-3p expression was contrary to these results. Overexpression of FKBP5 significantly upregulated AMPK, mTOR, and SREBP1 gene expression, while inhibition of FKBP5 expression was contrary to the above experimental results. Conclusion: In conclusion, these results indicate that bta-miR-365-3p may be involved in the AMPK/mTOR signaling pathway in regulating Yanbian yellow cattle preadipocytes differentiation by targeting the FKBP5 gene.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.220-220
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• 2017

Soybean (Glycine max (L.) Merill) is a very outstanding material crop with high protein and oil contents. We conducted this study to evaluate forage value and usability of soybean varieties as livestock forage. Three soybeans cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were evaluated for forage use in this study, and Kwangpyeongok and Yeongwoo were used as check forage corn and rice, respectively. The whole soybean plants were harvested at the R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing forage usability and quality. Days to harvesting of the R5 stage-OT93-26 was the shortest among the tested crops while that of Yeongwoo was 122 days. The fresh and dry matter yields of all 3 soybeans were greater at R6 stage than at R5. Crude protein of the soybean cultivars harvested had a higher compared to the rice and corn regardless of the harvesting stage. Contents of crude fiber, neutral detergent fiber and acid detergent fiber of Yeongwoo had the lowest whereas Pungwon harvested at R5 were the highest. Among the soybeans, digestible dry matter, dry matter intake, and relative feed value of R6-harvested Geomjeongsaeol and Pungwon were high more compared to those at the R5-harvested, but in case of OT93-26 those at R6 stage were inversely measured rather than those at R5 stage. In conclusion, soybean can be used as s forage with high nutritive value for livestock. Moreover, Geomjeongsaeol can be applied to develop new forage soybean varieties with high nutritive value, and R6 stage is the optimum harvesting stage in yield and quality of the tested soybeans more than R5.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.219-222
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• 2007

A total of the 261 Phytophthora infestans isolates collected from 2003 to 2005 in Korea were investigated for their physiological race composition. Among the isolates, we detected 18 physiological races and the dominant races were R0.1.3.5.6.10.11 and R0.1.3.5.6.7.10.11 with frequencies of 18.4% and 11.4%, respectively. All of the P. infestans races carried multiple virulence genes and showed virulence to the potato resistance genes R1, R3, R5, R6, R7, R10 and R11, but not to R8 and R9. Therefore, it is likely that the physiological races of P. infestans were diverse in Korea.

• Journal of the Korean Chemical Society
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• v.38 no.10
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• pp.769-773
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• 1994

Higher order structure of 5S rRNA isolated from Xanthomonas celebensis was examined using angiogenic extracted from milk. Angiogenin cleaved exclusively 3' P-O bonds on the far sides of pyrimidines in the single-stranded sequences of 5S rRNA. Whereas angiogenin acted only on the loop d of 5S rRNA at pH 7.0 in the presence of 10 mM $Mg^{2+}$, it acted on all the loops (a, b, c and d) except loop e in the absence of $Mg^{2+}$. In the absence of $Mg^{2+}$, bonds $U_{74}$-$G_{75}$, $U_{77}$-$A_{78}$ and $U_{103}$-$A_{104}$ were highly susceptible to the action of angiogenin both at pH 7.0 and at pH 3.5. On the other hand, at pH 3.5 in the absence of $Mg^{2+}$ angiogenin strongly cleaved the bond $C_{17}$-$G_{18}$ of loop a and the bond $U_{55}$-$G_{56}$ of loop b. The results lead us to the following conclusion. First, angiogenin can be used as one of the probes for the tertiary structure analysis of 5S rRNA. Second, the structure of loop d of 5S rRNA is variable depending on the concentrations of $Mg^{2+}$ and $H^{1+}$.

• Journal of Energy Engineering
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• v.15 no.3 s.47
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• pp.166-173
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• 2006

R134a is considered as an alternative refrigerant to R22 for air conditioners. An experimental investigation was made to study the characteristics of the heat transfer and pressure drop for R134a flowing in a fin-and-tube heat exchanger used for commercial air-conditioning units. Experiments were carried out under the conditions of inlet refrigerant temperature of $60^{\circ}C$ and refrigerant mass fluxes of $150,\;200,\;and\;250\;kg/m^{2}s$. The inlet air has dry bulb temperature or $35^{\circ}C$, relative humidity of 40% and air velocity varying from 0.68 to 1.6 m/s. Experiments show that air velocity decreased by 5.9% is needed for R134a than that of R22 while pressure drop for R134a was $18.1{\sim}20.4%$ higher than that of R22 for the degree of subcooling $5^{\circ}C$. The results are useful in designing more compact and effective condensers for various refrigeration and air conditioning systems using refrigerant R134a.

• Journal of radiological science and technology
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• v.8 no.2
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• pp.47-63
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• 1985

This paper was aimed to study the effects of Cobalt-60 gamma ray irradiation on Albino rat blood cells and the activity of enzymes were measured using blood cell auto analyzer (Cell-Dyn 900) and enzyme autoanalyzer (Gilford 3500) respectively. Cobalt-60 gamma rays those are grouped into 200R, 400R, 600R, 800R, 1,000R, 1,200R, 1,300R, 1,400R, 1,500R and 1,600R to the rats of male and female and measured the numeral varieties of blood corpuscles of the rats and the active varieties of enzyme of acid, alkaline phosphatase (ACP, ALP), lactic acid dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase. (GPT) those came out after 1, 3, 5 or 7 days. The results are summarized as follows: 1. In both case of male and female, the numeral varieties of the erythrocytes, in the 200R group of irradiation showed the minimum value in the 3 days group and a tendency of increase in the 5 and 7 days groups, while the numeral varieties of the leucocytes in every groups of irradiation showed a tendency of a rapid decrease after a day. The numeral varieties of the blood platelet in both case of male and female didn't show generally any great change in a day and 3 days groups, but showed a rapid decreasing appearance after the 5 days. 2. In both case of male and female, the activities of ACP were on the decrease gradually after 3 days of the irradiation and the activities of ALP were on the decrease after 5 days. Similarly in both case of mate and female, the activities of LDH showed the decrease after 3 and 5 days, and the phenomenous of GOT showed an appearance of increase in a day, but decreased after 3 days. As for the GPT activities couldn't find any great change in the male rats in comparison with the control groups, but in the female rats a tendency of the increase in 600R, 800R, 1,000R groups after 3 days. 3. In the case of the irradiation of high quantity of ray more than 1,200R, all rats died after 4 days, and by the irradiation of 1,600R all rats died after 3 days, and it showed the sensitive response of a living body to the irradiation of high quantity.