• Development and Reproduction
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• v.22 no.1
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• pp.39-53
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• 2018

The purpose of this study is to investigate the early life history of the Rhodeus fish, Rhodeus uyekii and R. ocellatus, in the Nakdong River to use as the preliminary data for the systematic study. The embryos used in the study were fertilized eggs (embryo) and larvae after artificial fertilization. The long diameter of the eggs of the R. uyekii was 3.39-3.97 mm (average $3.68{\pm}0.41mm$, n=30) and the short diameter was 1.36-1.55 mm (average $1.45{\pm}0.13mm$, n=30). The long diameter of the eggs of the R. ocellatus was 2.53-2.71 mm (average $2.62{\pm}0.12mm$, n=30) and the short diameter was 1.47-1.60 mm (average $1.53{\pm}0.09mm$, n=30). Hatching time was 48 hours for the R. uyekii and 50 hours for the R. ocellatus given that the average water temperature was $21.5^{\circ}C$. The hatched larvae were 4.95-5.00 mm (average $4.98{\pm}0.04mm$, n=5) for the R. uyekii and the total length was 3.66-3.69 mm (average $3.67{\pm}0.02mm$, n=5) for the R. ocellatus. R. uyekii was found to be 15.5-15.8 mm at total length (average $15.6{\pm}0.21mm$, n=5) on the 56 days after hatching with the number of dorsal fins being ⅲ-9, anal fins ⅲ-10, ventral fins ⅲ-5. The R. ocellatus was found to be 15.8-16.0 mm (average $15.9{\pm}0.14mm$, n=5) at total length on the 58 days with the number of dorsal fins being ⅲ-11, anal fins ⅲ-12 and ventral fins ⅲ-5 where the number of all fin stalks reached maximum.

• Korean Journal of Remote Sensing
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• v.4 no.1
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• pp.49-60
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• 1988

Principal Component Analysis (PCA) was applied for regional geological mapping to a multivariate data set of the Landsat TM data in the heavily vegetated and topographically rugged Chungju area. The multivariate data set selection was made by statistical analysis based on the magnitude of regression of squares in multiple regression, and it includes R1/2/R3/4, R2/3, R5/7/R4/3, R1/2, R3/4. R4/3. AND R4/5. As a result of application of PCA, some of later principal components (in this study PC 3 and PC 5) are geologically more significant than earlier major components, PC 1 and PC 2 herein. The earlier two major components which comprise 96% of the total information of the data set, mainly represent reflectance of vegetation and topographic effects, while though the rest represent 3% of the total information which statistically indicates the information unstable, geological significance of PC3 and PC5 in the study implies that application of the technique in more favorable areas should lead to much better results.

• Bulletin of the Korean Chemical Society
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• v.18 no.3
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• pp.305-310
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• 1997

The synthesis and characterization of the platinum heterocyclic carboxaldehyde thiosemicarbazone complexes [NC5H4CRNNC(S)NHR'PtCl] (R=H, R'=CH3(1); R=CH3, R'=CH3(2); R=CH3, R=H(3)) and diphenylphosphinophenyl carboxaldehyde thiosemicarbazone complexes [Ph2PC6H4CHNNC(S)NHRPtCl] (R=CH3(5); R=iC3H7(6); R=Ph(7)) are described. Compounds 1-3 were prepared by reaction of Pt(SEt2)2Cl2 with 2-acetylpyridine-4-alkylthiosemicarbazone in the presence of NEt3. Compounds 5-7 were prepared using Pt(SEt2)2Cl2 in toluene with diphenylphosphinophenyl carboxaldehyde alkylthiosemicarbazone. The compounds have been characterized by microanalysis, NMR (1H, 13C, 31P) spectroscopy, and single-crystal X-ray diffraction. X-ray single crystal diffraction analysis reveals that compound 5 is a mononuclear platinum compound with P,N,S-coordination mode.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.305-311
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• 1988

The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168 $spr^{r}$ chromosome at the frequency of $1.0\times 10^{-5}-5.0\times 10^{-7}$ and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin ($50{\mu}$g/ml) and spectinomycin ($100{\mu}$g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.

• Animal Bioscience
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• v.37 no.4
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• pp.609-621
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• 2024

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

• Animal Systematics, Evolution and Diversity
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• v.11 no.2
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• pp.207-221
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• 1995

Electrophoretic analysis was performed to determine the interspecific genetic relationships of the genus Raja collected in Korea waters. We confirmed 5 species [Raja (Okamejei) meerdervoortii Bleeker, R.(O).acutispina Ishiyama, R.(O) kenojei Muller et Henle, Raja (Dipturus) kwangtungensis Chu, and R.(D.) pulchra Liu] inhabting in Korea. As results of starch gel electrophoresis, 24 genetic loci were obtained from 13 enzymic and non-enzymic proteins, and allele frequencies of each locus were calculated . Even thought their various dorsal color patterns, the mean genetic similarity among 4 conspecific populations of R.(O) kenojei showed highly close genetic relationships (S=0.966) R.(O) kenojei and R.(D) kwangtungensis which belong to different subgenus , revealed subspecific level of differentiation (S=0.829). R. (Diptuuus ) kwangtungensis was genetically much closer to Raja(Okamejei) species than to Raja (Dipturus) species group. R.(O) acutispina and R.(D) kwangtungensis were unrecorded species to Korea.

• Journal of the Korean Chemical Society
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• v.46 no.1
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• pp.46-51
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• 2002

The recognition and cleavage of 5S rRNA from P. alcaligenes by metallopeptides to the form $Ni(II){\cdot}Gly$-Gly-His(Arg)COOH and $Cu(II){\cdot}Gly$-Gly-His(Arg)COOH were investigated. The results of RNA cleavage analyses suggest that metallopeptides selectively target the unpaired or unstably paired bases of stem-loop structure of 5S rRNA. The selectivity of metallopeptides was little affected by the species of metal ion, Ni(II) or Cu(II). When the result of cleavage by metallopeptides was compared with that of by metal complexes M(II)CR, the recognition by metallopeptides was more selective and structure specific. The cleavage data by metallopeptides and other metal complexes were used to probe the secondary structure of 5S rRNA from P. alcaligenes.

• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.921-932
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• 2021

LINC01272 is a long non-coding RNA (lncRNA) that has been considered as a biomarker for many diseases including lung squamous cell carcinoma. Here, we investigated the function and mechanism of LINC01272 on lung cancer (LC). The differential expression of LINC01272 in LC and normal samples was analyzed by GEPIA based on the data from TCGA-LUAD database, as survival prognosis was analyzed through Kaplan-Meier Plotter. LINC01272 overexpression plasmid and miR-7-5p mimic were transfected into A549 and PC-9 cells. LINC01272, miR-7-5p and cardiolipin synthase 1 (CRLS1) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction. Cell viability was detected through MTT assay. Cell multiplication was evaluated by cell formation assay. Cell apoptosis was assessed through flow cytometry assay. Through bioinformatics, the target miRNA of LINC01272 and downstream genes of miR-7-5p were predicted. The targeting relationship was tested by dual luciferase reporter analysis. CRLS1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) and cleaved caspase-3 protein levels were detected through western blot. LINC01272 was downregulated in LC and low LINC01272 expression had poor prognosis. In A549 and PC-9 cells, LINC01272 inhibited cell viability and multiplication and induced apoptosis. LINC01272 negatively regulated miR-7-5p and CRLS1 was a target of miR-7-5p. MiR-7-5p reversed the effect of LINC01272 on viability, multiplication, apoptosis and expression of miR-7-5p and CRLS1 as well as apoptosis-related factors (Bcl-2, Bax and cleaved caspase-3). LINC01272 suppressed cell multiplication and induced apoptosis via regulating the miR-7-5p/CRLS1 axis in LC.

• Journal of Sericultural and Entomological Science
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• v.9
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• pp.43-47
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• 1969

This experiment was carried out to investigate the effect of gamma ray on the eggs of later stage in late fall rearing season. The results are summarized as follows: 1. The duration of larval stage was simmilar to that of control in any dose. The silkworm growth was uniform in does of 4,000 gamma ray, but in does of 8,000r 16000r was irregular. There were not malformed silkworms but many small silkworm. 2. Mortality of silkworms was very high 8,000r and over; virus diseases of 87.6 per cent, flacherie of 5.9 per cent and complication of 6.5 per cent. 3. The more the dose of gamma ray became, the lighter the weight full grown silkworm and matured silkworm became. 4. The uniformness of cocoons was registered in dose of 2,000r and the cocoons were irregular or small in dose of 16,000r

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.116-124
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• 2017

This experiment was conducted to evaluate feed value and usability of soybean varieties as livestock forage. In this study, three soybean cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were harvested at R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing feed value of soybean. Days to R5 stage harvest of OT93-26 among the three soybean cultivars was 55 days and the shortest while Pungwon took 103 days to reach at R6 stage. The R6-harvested soybeans had higher dry matter (DM) yields and crude protein (CP) content than the R5-harvested. However, both DM and CP were the highest in the R6-harvested Geomjeongsaeol. Contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) of Pungwon harvested at R5 were the highest whereas the R6-harvested Geomjeongsaeol had the lowest. Digestible dry matter (DDM), dry matter intake (DMI), and relative feed value (RFV) of the R6-harvested Geomjeongsaeol and Pungwon were higher than those of the R5-harvested, but in case of OT93-26, those at R6 stage were low rather than those at R5 stage. However, soybean could be used as alternative forage with high feed value for livestock. Taken together, Geomjeongsaeol could be used for developing new forage soybean varieties with high feed value, and R6 would be the optimum harvesting stage for yield and quality of forage soybean.