This study was carried out to develop of environmental risk assessments and the biosafety guide for Vitamin E enhanced transgenic soybean at LMO (Living Modified Organism) isolation field. In LMO quarantine area of National Institute of Agricultural Sciences, insect species diversities and population densities on vitamin E enhanced transgenic soybean and non-GM soybeans (Willams 82 and Seoritae) were investigated. A total of 17,717 individuals of 77 species from 8 orders were collected in LMO isolation field. In three type soybeans field, total of 5,250 individuals in Vitamin E enhanced transgenic soybean, 5,510 individuals in Willams 82, and 6,957 individuals in Seoritae were collected, respectively. There was no difference between the population densities of insect pests, natural enemies and other insects on Vitamin E enhanced transgenic soybean and Willams 82, while natural enemies density on Seoritae was higher than on Vitamin E enhanced transgenic soybean, but insect pests density on Vitamin E enhanced transgenic soybean was higher. These results provided the insects diversity for risk assessment survey of Vitamin E enhanced transgenic soybean and suggested that the guideline could be useful to detect LMO crops.
Kim, Hyunji;Noh, SeongYu;Jeong, Hyun-Gi;Moon, Jeongsuk;Shin, Yuna;Lee, Kyung-Lak;Lee, Su-Woong;Lee, Jae-Kwan
Korean Journal of Ecology and Environment
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v.51
no.4
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pp.311-321
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2018
The purpose of this study is to investigate effect of stream channel naturalness on ecological health by using environmental factors and Fish Assessment Index (FAI) in the Han River of South Korea. These samples and data have been released from the research project titled Stream/River Ecosystem Survey and Health Assessment, which was conducted in 444 sites in the Han river watershed from 2008 to 2016. All samples were classified into five groups according to a degree of morphological changes of stream. Water chemistry analyses indicated a decline in water quality by decreasing stream channel naturalness, it is assumed that channelized stream was vulnerable to aquatic pollution compared to the natural meandering stream. In the result of frequency of dominant species, sensitive species and insectivore such as Zacco koreanus, Rhynchocypris kumgangensis and Pungtungia herzi were frequently dominated in the natural meandering stream while tolerant species and omnivores such as Carassius auratus and Cyprinus carpio were more dominated in the channelized streams. The FAI in the channelized stream shows decline to average of $46{\pm}25$ compared with that of the natural meandering stream ($80{\pm}20$). The decrease in FAI was highly influenced by changes in matrixes of fish assemblage structure such as number of sensitive species (M3), portion of omnivores (M5) and insectivores (M6). Moreover, annual average FAIs from 2008 to 2016 were significantly correlated with water chemistry, especially TN, TP and BOD ($r^2=0.59$, p<0.0001). Taken together, all the results suggest that the stream channelization could negatively impact on the water quality and fish assemblage structure, leading to degradation in aquatic ecosystem health.
Dongrae hot spring belongs to the residual magma type and has a long history of bathing since the Silla dynasty in Korea. Due to long development of hot spring water, it is expected that the amount of hot spring water in Dongrae hot spring has been changed. In this study, long-trem water level data of Dongrae hot spring were examined for recognizing the change of the hot spring. By the fluctuation analysis of the hot spring water level from January 1992 to July 2018, the maximum and minimum annual drawdowns of no. 27 well were 137.70 and 71.60 meters, respectively, with an average drawdown of 103.39 m. On the other hand, the maximum and minimum annual drawdowns of no. 29 well were 137.80 and 71.70 meters, with an average drawdown of 103.49 m. Besides, drawdown rate became bigger in recent years. As a result of analyzing autocorrelation of the two wells, the correlation coefficient ranged from 0.919 to 0.991, showing seasonal groundwater level fluctuation. The cross correlation analysis between water level and precipitation as well as water level and hot spring discharge resulted in the correlation coefficients of -0.280 ~ 0.256 and 0.428 ~ 0.553, respectively. Therefore, using Dongnae hot-spring water level data from 1992 to 2018, the Mann-Kendall test and Sen's test showed that the continuous decline of water level was mainly caused by the pumping of the hot spring water among various reasons.
Journal of agricultural medicine and community health
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v.46
no.2
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pp.78-88
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2021
Objectives: This study aimed to investigate health risk awareness pertaining to fine dust exposure and the use of face masks in farmers, as well as their attitude toward education regarding fine dust-related respiratory disease prevention. Methods: In total, 295 farmers were interviewed in a survey using a structured questionnaire to obtain data on general characteristics, farming-related characteristics, health risk awareness pertaining to fine dust exposure, attitude toward education on fine dust-related respiratory disease prevention and the use of face masks. This study was analyzed the correlation between the perceived susceptibility to fine dust exposure and willingness to participate in education on fine dust-related respiratory disease prevention. Results: The mean score for perceived susceptibility to fine dust exposure was 3.8 (out of 5), and the participants were highly willing to receive education on fine dust-related respiratory disease prevention. In Multiple response analysis of reactions to exposure to fine dust generated during work, 221 participants responded that they practiced at least one preventive action; participants gave a positive response to "wearing masks" (56.1%), "personal hygiene, such as hand washing." (52.9%). In terms of education methods, 94 (33.6%) participants preferred to learn online or via text messages. Conclusions: The significant correlation between the perceived susceptibility to fine dust exposure and willingness to participate in education on fine dust-related respiratory disease prevention shows the importance of promoting education on prevention. The results of this study can help understand as reference for education on fine dust-related respiratory disease prevention.
In this study, to reduce the production cost of poly(3-hydroxyalkanoates) (PHA), optimal cell growth and PHA biosynthesis conditions of the isolated strain Pseudomonas sp. EML8 were established using waste frying oil (WFO) as the cheap carbon source. Gas chromatography (GC) and GC mass spectrometry analysis of the medium-chain-length PHA (mcl-PHAWFO) obtained by Pseudomonas sp. EML8 of WFO indicated that it was composed of 7.28 mol% 3-hydrxoyhexanoate, 39.04 mol% 3-hydroxyoctanoate, 37.11 mol% 3-hydroxydecanoate, and 16.58 mol% 3-hydroxvdodecanoate monomers. When Pseudomonas sp. EML8 were culture in flask, the maximum dry cell weight (DCW) and the mcl-PHAWFO yield (g/l) were showed under WFO (20 g/l), (NH4)2SO4 (0.5 g/l), pH 7, and 25℃ culture conditions. Based on this, the highest DCW, mcl-PHAWFO content, and mcl-PHAWFO yield from 3-l-jar fermentation was obtained after 48 hr. Similar results were obtained using 20 g/l of fresh frying oil (FFO) as a control carbon source. In this case, the DCW, the mcl-PHAFFO content, and the mcl-PHAFFO yields were 2.7 g/l, 62 wt%, and 1.6 g/l, respectively. Gel permeation chromatography analysis confirmed the average molecular weight of the mcl-PHAWFO and mcl-PHAFFO to be between 165-175 kDa. Thermogravimetric analysis showed decomposition temperature values of 260℃ and 274.7℃ for mcl-PHAWFO and mcl-PHAFFO, respectively. In conclusion, Pseudomonas sp. EML8 and WFO could be suggested as a new candidate and substrate for the industrial production of PHA.
Jeon, Sung-Wook;Kim, Kwang-Ho;Lee, Gwan-Seok;Seo, Bo Yoon;Kim, Ji Eun;Kang, Wee Soo;Cho, Jum Rae
Korean Journal of Environmental Biology
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v.38
no.4
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pp.586-593
/
2020
This study was conducted to investigate the biological characteristics of Phanuromyia ricaniae (Hemiptera: Platygastroidea), an egg parasitoid of Ricania sp. (Hemiptera: Ricaniidae) as a biological control agent to control Ricania sublimata. The developmental period of P. ricaniae was 3.8 days for eggs, 11.1 days for larvae, and 16.3 days for pupae at 25℃, and 3.4 days for eggs, 7.8 days for larvae, and 15.3 days for pupae at 30℃. Except for the larval stage, the developmental periods were not significantly different, but the egg-to-pupa period at 30℃ was significantly shorter than that at 25℃. P. ricaniae emerged at the photophase in 24 hours, but not at the scotophase. A higher emergence rate (34.5%) was seen from 10:00 to 12:00 after the lights were turned. The female-to-male ratio of P. ricaniae was 3:1. The longevity of P. ricaniae adults was 49.0 days for females and 44.0 days for males at 20℃, 27.6 days for females and 28.4 days for males at 25℃, and 18 days for females and 14.0 days for males at 30℃. Its longevity at a low temperature (20℃) was longer than that at higher temperatures (25 and 30℃). Adult females laid eggs during all days except from 00:00-02:00 (scotophase time). The ovipositional distribution rate was 26.1% from 20:00 to 22:00, which was the peak, and the next peak was 15.7% at 10:00 to 12:00. P. ricaniae showed arrhenotokous parthenogenesis in which unfertilized eggs develop into males. Therefore, the results suggest that P. ricaniae may be a biological control agent for R. sublimata.
Kim, Jae Kyung;Jang, Dong Cheol;Kang, Ho Min;Nam, Ki Jung;Lee, Mun Haeng;Na, Jong Kuk;Choi, Ki Young
Journal of Bio-Environment Control
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v.30
no.1
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pp.1-9
/
2021
This study was conducted to examine the changes of photosynthesis, growth, chlorophyll contents and functional material contents in light intensity and EC concentration of wild baby leaf vegetable, Indian lettuce (Lactuca indica L. cv. 'Sunhyang') in DFT hydroponics. The cultivation environment was 25±1℃ of temperature and 60±5% of relative humidity in growth system. At 14 days after sowing, combination effect of light intensity (Photosynthetic Photon Flux Density (PPFD 100, 250, 500 µmol·m-2·s-1) and EC level (EC 0.8, 1.4, 2.0 dS·m-1) of nutrient solution was determined at the baby leaf stage. The photosynthesis rate, stomatal conductance, transpiration rate and water use efficiency of Indian lettuce increased as the light intensity increased. The photosynthesis rate and water use efficiency were highest in PPFD 500-EC 1.4 and PPFD 500-EC 2.0 treatment. The chlorophyll content decreased as the light intensity increased, but chlorophyll a/b ratio increased. Leaf water content and specific leaf area decreased as light intensity increased and a negative correlation (p < 0.001) was recognized. Plant height was the longest in PPFD 100-EC 0.8 and leaf number, fresh weight and dry weight were the highest in PPFD 500-EC 2.0. Anthocyanin and total phenolic compounds were the highest in PPFD 500-EC 1.4 and 2.0 treatment, and antioxidant scavenging ability (DPPH) was high in PPFD 250 and 500 treatments. Considering the growth and functional material contents, the proper light intensity and EC level for hydroponic cultivation of Indian lettuce is PPFD 500-EC 2.0, and PPFD 100 and 250, which are low light conditions, EC 0.8 is suitable for growth.
Journal of the Korean Applied Science and Technology
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v.37
no.6
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pp.1517-1527
/
2020
This study is about the UV protection effect and water resistance of a multiple emulsion (W/O/W) sunblock cream applied with emotional engineering and reports an actual industrial case. Multiple emulsion system of sunblock cream has the characteristics of changing to a W/O type that is soft and moist when applied, and has excellent water resistance after absorption. Multiple emulsion cream is a highly functional sunblock cream that has both moisture and water resistance. It is a stable milky white cream with a viscosity of 36,000 cps. The organic sunscreen used for the sunscreen was ethylhexylmethoxycinnamate and bisethylhexyloxyphenolmethoxyphenyltriazine. Hexagonal zinc oxide and titanium dioxide that block both UVB and UVA were used. As a result of measuring the UV protection effect by the in-vitro method, the UV protection effect (SPF) is 78.9 for multiple emulsion cream, 76.7 for W/O cream, and 71.3 for O/W cream. It was found that the blocking effect was different. This obtained the highest effect value in the multiple emulsion. As a clinical (in-vivo) result of the UV protection effect, the SPF value representing the UV protection effect of the sunblock cream developed with a multiple emulsion system was 85.7, and the PA-value that blocks the UVA area was 26.5, and ++++. It was found that it has a corresponding high blocking effect. As a result of the water resistance test, the W/O/W formulation had a high waterproofing resistance of 93.8% even after 4 hours, W/O had 75.4%, and O/W had a low water resistance of 25.3%. In the results of the HUT test, it was found in the order of multiple emulsion sun block cream > hydrophilic cream > lipophilic cream. Based on the research results of this multiple emulsion, it is expected to be highly active as a sunblock cream dedicated to outdoor activities by improving the feeling of use, UV protection index, and water resistance. Therefore, in this study, a multiple emulsion system of sunblock cream is developed and has a characteristic that changes to a W/O type that has a soft and moist feeling when applied, and has excellent water resistance after absorption.
Journal of The Korean Society of Grassland and Forage Science
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v.41
no.4
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pp.250-258
/
2021
This study was conducted to investigate the effect of feeding systems on feed intake, weight gain, and fawn performance (Cervus canadensis) and estimation of grazing intensity in Elk doe at pasture. A sixteen Elk doe about 236.2 kg were randomly assigned to two feeding treatments. The treatment consisted of a barn feeding system (BF) and grazing at pasture (GR), and pasture was mainly composed of tall fescue, orchard grass, and Kentucky bluegrass. The moisture content of pasture was 19.51~22.61%, which was similar during experimental periods. The crude protein content was significantly higher from June to July (p<0.05). The contents of neutral detergent fiber and acid detergent fiber ranged 53.65~60.18%, and 26.08~29.10%, respectively. There were no significant differences between feeding systems on supplementary feed intake, but the roughage and total dry matter intake showed significant differences between treatment groups (p<0.05), except for May. In August, roughage intake was dramatically decreased in the GR group due to summer environmental changes. On the other hand, the higher intake of roughage in September might be related to nutrient intake for mammals. There was no difference in body weight between treatment groups, but the fawn performance was significantly higher in the GR group (p<0.05). These results might be suggested that grazing elk doe might positively affect fawn growth. However, it is considered that BF might increase the deer weaning rate during the parturition period, since the lower weaning rate in the GR group compared to the BF group. The grazing intensity of Elk doe was increased from May to July and decreased in August, which was influenced by pasture productivity. The average grazing intensity of Elk doe was found to be 15 heads/ha, which might be controlled by supplementary feeding. Further studies needed that mixed sowing methods and fertilization management in old grazing pastures for improved pasture productivity.
Validation of satellite data products is critical for subsequent analysis that is based on the data. Particularly, performance of ocean color products in turbid and shallow near-land ocean areas has been questioned for long time for its difficulty that stems from the complex optical environment with varying distribution of water constituents. Furthermore, validation with ship-based or station-based measurements has also exhibited clear limitation in its spatial scale that is not compatible with that of satellite data. This study firstly performed validation of major GOCI-II products such as remote sensing reflectance, chlorophyll-a concentration, suspended particulate matter, and colored dissolved organic matter, using the in-situ measurements collected from ship-based field campaign. Secondly, this study also presents preliminary analysis on the use of drone images for product validation. Multispectral images were acquired from a MicaSense RedEdge camera onboard a UAV to compensate for the significant scale difference between the ship-based measurements and the satellite data. Variation of water radiance in terms of camera altitude was analyzed for future application of drone images for validation. Validation conducted with a limited number of samples showed that GOCI-II remote sensing reflectance at 555 nm is overestimated more than 30%, and chlorophyll-a and colored dissolved organic matter products exhibited little correlation with in-situ measurements. Suspended particulate matter showed moderate correlation with in-situ measurements (R2~0.6), with approximately 20% uncertainty.
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