• Journal of Embryo Transfer
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• v.19 no.2
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• pp.113-119
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• 2004

The purpose of the present study was to examine whether collection time affects results of oocyte recovery from superovulated goats. Fiftyty-one mature Korean native goats, maintained in a pen under natural day length and fed hay ad libitum, were pretreated with progestagen impregnated CIDR for 10 days and then the goats were divided into two groups. One group of the goats received a single intramuscular injection of 1,000 IU PMSG on Day 8 of CIDR insertion. The other group of the goats received twice daily intramuscular injections of a total of 70 mg FSH for 3 days from Day 8 of CIDR. All the gonadotropin treated goats were injected with 10 mg $PGF_{2\alpha}$ on Day 8 and 400 IU hCG in the afternoon on Day 10. For oocyte recovery, donor goats were fasted 24 h before operation. Anesthesia was induced by intravenous injection of 2% xylazine(0.2 mg/kg body weight) and ketamin(11 mg/kg body weight). In vivo oocytes were recovered by follicle aspiration or oviduct flushing at 29 to 34, 35 to 40 and 41 to 50 h after hCG injection through mid-ventral incision. There was no significant difference in the mean number of CL and oocytes recovered. Oocyte collection at 29 to 40 h after hCG increased(P<0.05) the recovery rate of ovulated oocytes in oviducts compared to 41 to 50 h. The same results were also observed in the recovery of follicular oocytes. Oocyte grade was not affected by collection time. When oocytes were collected from follicular oocytes at 41 to 50 h after hCG, the recovery rate of Grade II oocytes was the lowest(P<0.05). From these results, it is suggested that oocyte recovery at 35 to 40 h after hCG will be successful for further use.

• Korean Journal of Ichthyology
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• v.13 no.3
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• pp.161-165
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• 2001

Artificial fertilization (dry method) of mature right-eye flounder, Limanda yokohamae (female : 25.1~30.4 cm in total length (TL), male : 24.5~28.5 cm in TL), obtained from the fish market in Dolsan-do, Yosu was performed in the Fisheries Exhibition. Hatched larvae and juveniles were reared to describe their morphological development. Newly hached larvae attained 3.13~3.42 mm in TL. Their eyes were yellowish brown and their mouth and anus were not yet opened. Three to six days after hatching the larvae attained 3.35~4.61 mm in TL. Their mouth and anus were open and the yolk sac was almost absorbed. Feeding activity increased as the mouth became larger. At 25 days, the larvae attained 5.47~5.91 mm in TL. The caudal notocord was flexed $45^{\circ}$ upward. At 35 days, the larvae attained 6.83~7.60 mm in TL. Rays of the dorsal and anal fins were formed, and the left eye was moved slightly to the right side of the head. At 55 days, the juveniles attained 9.38~11.73 mm in TL. The left eye was moved completely onto the right side. All of the fins had complete set of the fin rays, and the juveniles spent most of the time on the bottom resting on their blind side (D. 68~70 : A. 50~52 : P. 11: V. 6 : C. 18~19).

• Research in Plant Disease
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• v.8 no.3
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• pp.166-170
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• 2002

Powdery mildew of mug bean was found in Suwon on September 2000. Many white and powdery mycelial colonies appeared on leaves of the plants. Conidia with fibrosin bodies were ellipsoid to elongate-ellipsoid, 25~38$\times$16~22 ${\mu}{\textrm}{m}$ in size and farmed in chains. Conidiophores were erect on superfical mycelium, 67~100 ${\mu}{\textrm}{m}$ in length. Foot cells were cylindric with 37~65$\times$10~11 ${\mu}{\textrm}{m}$ in size. Appressoria were indisdinct. Germ tubes from conidia were simple, not branched. Dark brown cleistothecia were observed on the leaves of mung bean. Cleisto-thecia were globose or subglobose, 85~1130 ${\mu}{\textrm}{m}$ in size. Wall cells were irregularly polygonal with 15~45$\times$7~26 ${\mu}{\textrm}{m}$. Appendages were mycelioid, 6-11 per ascocarp, coloured, throught when mature or paler upwards, 1~7 septa, 192~223$\times$5~7 ${\mu}{\textrm}{m}$ in size. A single sun had in a cleistothecium and was ovoid or subglobose with 62~95$\times$50~67 ${\mu}{\textrm}{m}$ in size. Ascusspore had 8 spores in an ascus and rarely developed, mostly imatuna ellipsoid-ovoid with 16~24$\times$12~16 ${\mu}{\textrm}{m}$. On the basis of morphological characters of the conidial stage and teleo-morph, the fungus was identified as Sphaerotheca phaseoli.

• Korean Journal of Ichthyology
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• v.20 no.4
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• pp.263-271
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• 2008

The maturity and spawning of Korean flounder Glyptocephalus stelleri were investigated using samples randomly collected in the East Sea of Korea from May 2005 to September 2008. Gonadosomatic index began to increase in December and reached a maximum between March and May. After spawning it began to decrease from June. The spawning period was March to June, and the main spawning period was April to May. Annual reproductive cycles of this species can be divided into four successive stages; immature stage (July~November), maturing stage (December~February), mature stage (March~April) and spent stage (May~June). The fecundity ranged from 15,146 eggs at 24.1 cm (TL) to 101,491 eggs at 38.1 cm (TL). The relationship between total length (TL) and fecundity (F) can be expressed as $F=0.0004TL^{3.449}$ ($R^2=0.663$), with F increasing with TL. The TL at 50% group maturity ($TL_{50%}$) was estimated to be 25.6 cm.

• Korean Journal of Ichthyology
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• v.28 no.4
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• pp.239-248
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• 2016

The habitat, age and spawning characteristics of the Korean eastern spined loach, Iksookimia yongdokensis, were investigated to obtain baseline data for its ecological characteristics in the Chuksancheon (stream), Yeongdeok-gun, Gyeongsangbuk-do, Korea. The difference between the habitats of juveniles (0+) and 1~4 year old loaches was that juveniles mainly inhabited sandy bottoms (substratum particle size $0.6{\pm}1.44\;cm$) with very slow velocities of water current ($2.4{\pm}2.09\;cm/sec$) and low depths ($29.2{\pm}7.57\;cm$), whereas 1~4 year old loaches inhabited pebble and cobble bottoms (substratum particle size 5.5~6.1 cm) with relatively fast velocities of water current (3.8~4.6 cm/sec) and deep depths (50~70 cm). The age groups for I. yongdokensis (female) estimated by the frequency distribution of total length in the spawning season (July) indicated that the 35~64 mm is 1-year old, the 65~94 mm group is 2-year old, the 95~119 mm is 3-year old, and the 120~145 mm is more than 4-year old. The sex ratio (♂/♀) was 0.69, and females were 10~30 mm larger than males. The lamina circularis at the base of the pectoral fins in males, a secondary sexual characteristic, formed at the 13th month after hatching. The spawning season according to the gonadosomatic index (GSI) was June to July with water temperatures of $20{\sim}25^{\circ}C$. The average number of eggs was $2,292{\pm}618$, with a diameter of $1.46{\pm}0.02\;mm$ for mature eggs.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.1
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• pp.50-55
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• 1986

To get the basic information about ginseng seedling production, yields of ginseng seedling and soil physico-chemical components in 32 fields of Yang-Jik and 19 fields of Ban-Yang-Jik nursery were investigated. Germination rate is $78.5{\pm}3.0%$, $77.8{\pm}2.8%$ in Yang-Jik and Ban-Yang-Jik. Rate of number of mature seedlings to number of seeds planted is $62{\pm}13.5%$, $53{\pm}12.6%$ in Yang-Jik and Ban-Yang-Jik. Rate of number of available seedlings to number of seeds planted (Rate of available seedling) is $42{\pm}12.5%$, $26{\pm}12.1%$ in Yang-Jik and Ban-Yang-Jik. The number of available seedlings per Kan (Kan means $180{\times}90cm$ area) is $627{\pm}187$, $368{\pm}182$ in Yang-Jik and Ban-Yang-Jik. Rate of number of unusable seedlings to number of mature seedlings is 34%, 51% in Yang-Jik and Ban-Yang-Jik. It showed the negative correlation between amount of coarse sand and stem height, and central leaf length, on the other hand, possitive correlation between amount of extremely fine sand, and stem height, and central leaf length respectively. Rate of available seedling was positively correlated with amount of medium, fine and extremely fine sand, whereas negatively correlated with amount of coarse sand in Yang-Jik. Root weight per seedling was positively correlated with amount of medium, fine and extremely fine sand. Contents of organic matter, available phosphate and potassium in Ban-Yang-Jik were higher than those of Yang-Jik, but content of lime was higher in Yang-Jik compared to that of Ban-Yang-Jik. Possitive correlations were showed between leaf area per seedling and content of organic matter, and between stem height and content of lime in Yang-Jik. Root weight per seedling was positively correlated with content of organic matter and magnesium in Ban-Yang-Jik, but there is no correlation between any of soil chemical components and root weight in Yang-Jik.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.563-574
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• 1986

The structure of gonads, gametogenesis and reproductive cycle of the jackknife clams, Solen strictus and Solen gordonis were investigated mainly by histological observation. The first species used were monthly sampled at the coastal area of Dadaepo, Pusan, Korea and Naechodo, Kunsan, Korea for one year from February 1982 to January 1983. The second species were monthly sampled at the sand beach of Dadaepo, Pusan, Korea, from February 1982 to January 1983. Sexualities of Solen strictus and Solen gordonis are dioecious, and these species are oviparous. The gonads are irregularly arranged from the subregion of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary was composed of a number of small ovarian sacs and the testis was composed of several testicular lobuli which from the tubular structure. Early multiplicating oogonium was about $10{\mu}m$ in diamater. Nucleus and nucleolus, at that time, were distinct in appearance. Each of the early growing oocytes made an egg-stalk, connected to the germinal epithelium of the ovarian sac. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed in the ovarian sacs in the early development stages. With the further development of gonad, these tissue and cells gradually disappeared. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells function as nutritive cells in the formation and development of the early stage germ cells. Mature oocytes were free in the lumen of ovarian sacs and gradually become round or oval. Ripe oocyte was about 80 to $90{\mu}m$ in diameter. With the further development of testis, each of the testicular lobuli formed stratified layers composed of spermatogonia, spermatocytes, spermatids and spermatozoa in groups on the germinal epithelium. After spawning, the gonad gradually degenerated, and disorganized completely. Then new differentiated tissues were rearranged next year. The annual reproductive cycle of those species could be classified into five stages; multiplicative, growing, mature, spent, degenerative and resting stage. It seems that the spawning season is closely related to the water temperature, and the spawning of Solen strictus occurs from June to July at above $20^{\circ}C$ in water temperature. The peak spawning season appeared in June at Dadaepo and in July at Kunsan, The spawning of Solen gordonis occurs from May to June with the peak spawning season in June. Percentages of the first maturity in female of Solen strictus ranging from 5.1-6.0 cm and 7.1-8.0 cm in shell length were $50\%$ and $100\%$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.5
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• pp.449-470
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• 1984

The cercaria of Bacciger herengulae which is parasitized on the gonad of Solen strictus was investigated in order to reveal its entire life history. The area covered for the study was in the vicinity sea of Naechodo, the estuary of the Kum river in the western coast of Korea during the period of 1980-1983. Morphology and development as well as infection rates of sporocyst and cercaria within Solen strictus were examined. For accomplishing the objectives of this study, an artificial infection experiment and some investigations on the second intermediate host, the final host and the growing stages were also studied in both laboratory and natural habitat of Solen strictus. According to the study, it was revealed that the first intermediate hosts were Meretrix lusoria, Solen strictus, Tapes japonica and Laternula limicola, the second intermediate host was Palaemon (Exopalaemon) carinicauda and the final hosts were Konosirus punctatus and Harengula zunasi. A mature sporocyst which was found in the gonad of Solen strictus was $4.0-4.3{\times}0.2-0.21\;mm$ insize, and the cercaia with 27 pairs of setae, each seta consisting of 6 tufts, was $270{\times}147{\mu}m$ in body size and $550{\times}52{\mu}m$ in tail size. Oral sucker($52{\times}42{\mu}m$), pharynx, vental sucker and two testese were obviously seen within the cercaria. The excretory vesicles of cercaria were in V-shape and the flame cell were formula was expressed as 2[(3+3)+(3+3)]=24. The infection of cercaria in the first intermediate host, Solen strictus, was found throughout the year regardlless of the water temperature, and its mean infection rate was $9.67\%$ during the study period. The infection rate fluctuated with temperature, the highest being $28.0\%\;at\;28.0^{\circ}C$ water temperature in July and the lowest $2.4\%\;at\;19.5^{\circ}C$ in October, and it increased in proportion to the shell length on the host. But cercaria was not detected at below 4.0 cm in size of the host. Mature cercariae were found 6 months from May to October when water temperature was above $19.5^{\circ}C$. On the other hand, when water temperature was below $19.5^{\circ}C$, only immature cercariae and sporocysts were found. The cercariae were active for 35 hours and survived for 71 hours at $20^{\circ}C$, and 29 and 34 hours at $25^{\circ}C$ respectively, whereas the cercariae were inactive at less than $20^{\circ}C$ in water temperature. Cercaria, from Solen strictus, approached shrimp of 1-3 cm in body length as its second host. Then, it began to intrude in to the muscle of shrimp after 2-3 hours. The infected cercaria formed cyst after 7-8 hours, and became mature metacercaria. $420{\times}310{\mu}m$ in size, 15 days afer infection. The infection rate of metaceria to shrimp in the laboratory was highest, at $25^{\circ}C$ being $61\%$ and at $20^{\circ}C\;17%$. The infection rate of metacearia in shrimp was highest in the first abdominal segment, followed by cephalothorax, the second, and fifth abdominal segments, and in that order. Also, the infection rate of metacercaria in wild shrimp was high $9.6-11.1\%$ at $26.5^{\circ}C$ in June, and low $1.56-2.5\%$ at $28-29.5^{\circ}C$ from July to August. The infected shrimp with metacercaria was experimentally fed to Konosirus punctatus in the laboratory in order to know its final host. The metacercaria developed into the adult worm, $440-520{\times}310-360{\mu}m$ in size, within the intestine of Konosirus punctatus 20 days after infection. The adult worm was oval shape and $20-24{\times}11-20{\mu}m$ in size. The infection rate of adult worm to Konosirus punctatus and Harengula zunasi ranged 87.3 to $100\%$, the mean being $95.2\%$, regardless of the body length of their hosts. The infection rate was $100\%$ in June and July, but it decreased in September and October. The size and body structure of the trematode observed during the present study were well agreed with those ievestigated by Yamaguti(1938), thus, it may be concluded that the adult worm it identified as Bacciger harengulae.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.1-8
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• 2012

This study was carried out in 2010 to investigate the utilization and forage quality of native grasses, such as $Phragmites$ $communis$ which might reduce the cost of feeding domestic cattle. The regions surveyed were the Haenam ranch in Haenam, Pyeongchang, Wonju, and Yeonggwang. In Haenam, yearly silage production harvested from 300 ha was 2,000 MT (7 MT/ha). All of those round bale silages were self-consumed in that region, and marketing price was 50,000~55,000 won per roll (110 won/kg). $Phragmites$ $communis$ of 150 cm in length contained 8.4% crude protein (CP) with relative feed value (RFV) 71.9 and 60.1% in vitro dry matter digestibility (IVDMD), which was somewhat more favorable than forage quality of rice straw. The silage quality of $Phragmites$ $communis$ varied greatly by the time of harvest (CP 4.7~6.4%, RFV 62.2~78.9, and IVDMD 41.9~53.9%), even with the same district and of the same original forage materials. Generally, the quality of $Phragmites$ $communis$ silage of was poorer than that of sorghum ${\times}$ sudangrass hybrid. In Pyeongchang, forage quality of $Phragmites$ $communis$ was decreased from 13.9% to 7.6% in CP, 90.7 to 76.1 in RFV, and 72.9% to 54.7% in IVDMD, as plant was getting mature, from 79 cm to 117 cm, 121 cm or to 142 cm in length. In Wonju, the quality values of $Phragmites$ $communis$ of 130 cm in length were 8.5% CP, 82.3 RFV and 70.2% IVDMD, while those of matured grasses of 220 cm in length were lower (10.2% CP, 65.1 RFV and 48.9% IVDMD), but this was a little more favorable than quality of rice straw. In Yeonggwang, feeding $Phragmites$ $communis$ was tried in a Hanwoo feed, but stopped due to low profitability. In conclusion, the overall quality of most native grasses including $Phragmites$ $communis$ in this survey was poor. Therefore, we recommend that $Phragmites$ $communis$ and native grasses should be harvested on June or July to obtain richer forage quality in forage values than rice straw.

• The Korean Journal of Malacology
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• v.30 no.2
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• pp.107-116
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• 2014

Gonadal development and maturation mechanism were studied on the freshwater marsh clam Corbicula papyracea (Heude), which is the endangered species in Korea. The specimens were collected in the rearing ponds and waterway of NFRDI in Gapyeong-gun, Gyeonggi-do from January to December 2004, and then investigated by condition factor, relative growth, gonadal development phases and gonad histological characters based on 30-50 individuals every month. Comparing with the freshwater marsh clam, C. papyracea is small, light olive brown shell and violet interior. The hermaphrodite individuals of C. papyracea take an internal fertilization and fertilized eggs are stored in the foster-sack in the gills, then the hatched juveniles are released outside after an ovoviviparous process. The average water temperature of inhabit area was in range of $1.8-27.0^{\circ}C$ and usually took great effects on the gonad maturation of C. papyracea. The condition factor ranged from 0.14 to 0.21 throughout the year, which was the lowest during winter season (December-February), and gradually increased to the highest value of 0.21 in May. The ratio of meat weight to total weight was 25.9-38.7%, indicating the similar trend with condition factor. The highest values of condition factor and the ratio of meat weight appeared 1-2 months later than gonadosomatic index reached the peak value, it was probably because that the ovoviviparous eggs would spend a long period before hatching from the foster-sack in the gills. To synthesize the characters of meat weight, condition factor and gonad development by histological study, reproductive cycle of C. papyracea could be divided into five successive stages: multiplicative stage (December to February), growing stage (February to May), mature stage (June to August), spawning stage (August to November), recovery stage (November to December). The smallest shell length of matured C. papyracea was 12.6 mm, and individuals, larger than 16 mm, was formed the nursery in the gills.