• 한국식품영양과학회지
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• 제34권1호
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• pp.1-7
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• 2005

DPPH radical 소거 활성은 추출물의 농도가 증가함에 따라 높게 나타났으며, 추출용매별 radical 소거활성은 거의 유사한 경향으로 나타났다. 프로폴리스 추출물의 첨가농도가 증가함에 따라 환원력이 증가하는 경향을 나타내었으며, 특히 50% 에탄을 추출물이 가장 강한 환원력을 나타내었다. 유지에 프로폴리스 추출물을 첨가하여 과산화물가, TBA가 및 산가를 측정한 결과 95% 에탄을 추출물이 가장 높은 산패 억제능을 나타내었다. 프로폴리스 추출물의 농도가 증가함에 따라 아질산염 소거 활성이 증가하였으며, 물추출물 500 $\mu$g을 첨가하였을 때 90%의 가장 높은 활성을 나타내었다. 프로폴리스의 각종 추출물을 이용하여 항균활성을 측정한 결과 50%에탄을 추출물에서 가장 높은 활성을 나타내었으며, 특히 Bacillus subtilis균과 Bacillus cereus균과 같은 그램 양성균에서 높은 활성을 나타내었다. 프로폴리스의 각종 추출물을 이용하여 1, 10 및 100 $\mu$g/mL 농도로 각각 첨가한 후 A549(폐암세포)에 대한 in vitro에서의 세포독성을 조사한 결과 70% 에탄을 추출물 100 $\mu$g/mL를 첨가하였을 때 62.63%의 가장 강한 세포독성을 나타내었으며,95% 에탄올 추출물, 물추출물 및 50% 에탄을 추출물 순으로 나타났다.

• 한국태양에너지학회 논문집
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• 제31권6호
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• pp.66-71
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• 2011

Various single solvents were tested to find the effective solvent for the extraction of algae oil from wet-form Chlorella minutissima. In the case of single solvents, their extractabilities for algae oil were increased with their polarity because the water in wet algae cell is to form a solvent shell around the lipids. Based on these results, the wet-form algae samples were treated with a polar alcohol solvent and then a nonpolar solvent was added in algae residue. In the algae oil extraction by ethanol/n-hexane, total lipid contents were 40-50% and composition of triglyceride in extracted oil was 46.50%. Considering solvent toxicity of conventional solvent mixture such as chloroform and methanol for algae oil extraction, the ethanol/n-hexane system was identified as the effective one for the oil extraction from wet-form Chlorella minutissima.

• 한국식품저장유통학회지
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• 제7권2호
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• pp.177-183
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• 2000

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83$^{\circ}$C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.

• 한국수산과학회지
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• 제56권6호
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• pp.833-840
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• 2023

In this study, the antioxidant and anti-skin aging properties of the Korean marine algae Ishige foliacea were investigated. Solvent extracts from I. foliacea were prepared with 70% ethanol, 80% methanol, and water. The extraction yields of various solvent extracts ranged from 9.55% to 35.12%. In terms of antioxidant activity, the ethanol extract showed the highest ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activity, nitrite oxide scavenging activity, reducing power, and FRAP (ferric reducing antioxidant power). Regarding anti-skin aging activity, evaluation of the skin whitening and anti-wrinkle activities revealed that the methanol, water, and ethanol extracts possessed the highest tyrosinase (IC₅₀=0.98 mg/mL), elastase (IC₅₀=0.15 mg/mL), and collagenase (IC₅₀=0.06 mg/mL) inhibitory activities, respectively. These results suggest that I. foliacea holds potential as an antioxidant and anti-skin aging substance in food and cosmetic materials.

• 산업기술연구
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• 제27권B호
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• pp.161-167
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• 2007

The objetives of this study are to set up optimum extraction temperature, time and organic solvent for propolis extraction, to investigate chemical properties, and to develop health foods from propolis preparation. In this study, ethanol and ultrasonic extracts method performed to optimum extraction temperature was at 60, $20^{\circ}C$, optimum extraction time was at 12, 4 hours and optimum extraction amount of solvent was at 20, 15 times of propolis weight. When various ethanol solutions were used, whereas flavonoid content was highest in 70, 80% aqueous ethanol, respectively. So the ultrasonic extracts method used gave better results than the ethanol extracts method in this work. Extraction of propolis with etanol and ultrasonic extracts method was performed by using the water and various concentrations of aqueous ethanol as solvent. Sensitivity of propolis samples to Staphylococcus aureus was investigated and the results were shown. Samples of water extract did not inhibit microbial growth, where as 50% aqueous ethanol extract the largest inhibitory zone for Staphylococcus aureus, then decreased inhibition with increasing ethanol concentrations.

• 한국재료학회지
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• 제16권9호
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• pp.533-537
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• 2006

The effects of binary solvent mixtures with various ratios of toluene and ethanol on the properties of slurries and green sheets were investigated. Viscosity of slurry was changed by varying the ratio of solvent mixture which affected the solubility of binder. The relative solvency behavior of a solvent mixture could be predicted with the Hildebrand solubility parameter(${\delta}$) and hydrogen bonding index( ${\gamma}$). The minimum viscosity, the best dispersion of binder, was reached at the composition of toluene:ethanol=4:6, which corresponded to our forecast. The mechanical properties of green sheets related to evaporation of solvents were influenced by the composition of the solvent mixture. At the azeotrope the skin was formed on a drying cast during the drying process because of fast evaporation. At a range of concentrations over 50wt% toluene, green sheets could not be fully dried at low temperature due to excessive toluene. The mechanical properties of green sheets were excellent at the azeotrope-like composition of toluene:ethanol=4:6 which has a little excess of toluene over the azeotrope.

• 한국식품영양과학회지
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• 제40권2호
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• pp.316-320
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• 2011

미나리 에탄올 추출물과 용매분획물에 대한 항산화물질과 항산화활성을 분석한 결과 미나리 에탄올 추출물의 총 폴리페놀 함량은 37.50 mg GA eq/g이었으며, 에틸아세테이트 층에서 240.61 mg GA eq/g로 높게 나타났다. 총 플라보노이드 함량은 에탄올 추출물에서 26.50 mg catechin eq/g 이었지만 에틸아세테이트 층에서는 105.57 mg catechin eq/g의 함량을 나타내었다. DPPH법에 의한 항산화활성의 $IC_{50}$ 값은 에탄올 추출물에서 1.07 mg/mL이었으며, 에틸아세테이트 분획물에서는 0.08 mg/mL로 높았다. 총 항산화력은 에탄올 추출물에서 34.48 mg AA eq/g이었으며, 에틸아세테이트 층에서는 382.00 mg AA eq/g으로 높았다. 환원력은 1 mg/mL의 농도에서 에탄올 추출물은 0.45이었지만, 에틸아세테이트 층에서는 0.75로 높았다. Ferrous ion chelating 효과는 에탄올 추출물에서 32.01%로 높았고 용매 분획물에서는 오히려 감소하거나 없는 것으로 나타났으며 hydroxyl 라디칼 소거능의 $IC_{50}$은 에틸아세테이트 분획물에서 $26.71\;{\mu}g$/mL로 가장 높았다.

• Applied Biological Chemistry
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• 제48권1호
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• pp.89-92
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• 2005

삼백초 지상부의 추출조건을 설정하고자 추출용매 및 시간에 따른 이화학적 특성을 살펴보았다. 추출용매 조건은 40% 에탄올을 사용하여 추출할 때로 나타났으며, 이때, 총수율, 전자공여능, 총페놀함량, 총플라보노이드 함량은 각각 13.50%, 83.50%, 2.60 mg/ml 및 2.09 mg/ml으로 나타났다. 최적의 추출 시간은 4시간으로 나타났으며, 이때, 총수율, 전자공여능,총폐놀함량, 총플라보노이드 함량은 각각 12.79%, 71.13%, 2.83 mg/ml 및 2.16 mg/ml으로 나타났다. 삼백초 지상부를 40% 에탄을 추출물로 4시간 추출하였을때, quercetin 함량은 360.13 mg/100 g으로 나타났으며, quercetin 함량은 1379.54 mg/100 g으로 나타났다. 삼백초 지상부를 이용하여 추출조건을 설정한 결과 40% 에탄올을 이용하여 4시간 추출하는 것이 가장 적합한 것으로 나타났다.

• 대한의생명과학회지
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• 제11권4호
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• pp.517-523
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• 2005

The purpose of this study was to determine the effect of rat intestinal a-glucosidase inhibitor; methanol $(80\%)$, ethanol $(80\%)$ and water extract of Schizandra chinensis in Korea (KS: Schizandra chinensis in Korea) and China (CS: Schizandra chinensis in China). When the final concentration was 1 mg/ml for each sample (KS and CS), methanol extract of KS ($IC_{50}$ 1.62 mg/ml) showed $46.8\%$, ethanol extract of KS ($IC_{50}$ 1.48 mg/ml) showed $47.4\%$, water extract of KS ($IC_{50}$ 1.72 mg/ml) showed $46.3\%$ and methanol extract of CS ($IC_{50}$ 8.35 mg/ml) showed $13.3\%$, ethanol extract of CS ($IC_{50}$ 8.05 mg/lml) showed $16\%$, water extract of CS ($IC_{50}$ 8.37 mg/ml) showed $11.54\%$ of inhibitor for p-nitrophenyl $\alpha-D-glucopyranoside$ (pNPG) $\alpha-glcosidase$ activity, respectively. And the contents of total phenol, flavonoid of Schizandra chinensis were measured. When the final concentration was 1mg/ml for each sample (KS and CS), total phenol and flavonoid in KS were higher than CS, respectively. The order superoxide dismutase (SOD) activity $IC_{50}$ values of each solvent extracts of KS were: 2.006 mg/ml methanol extract, 2.304 mg/ml ethanol extract and 2.5 mg/ml water extract, which were higher than that of each solvent extracts CS as: 2.881 mg/ml methanol extract, 3.085 mg/ml ethanol extract and 3.190 mg/ml water extract.

• Journal of Forest and Environmental Science
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• 제36권1호
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• pp.62-66
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• 2020

Ethanol fermentation of the enzymatic hydrolysates from the products pretreated using 1-ethyl-3-methyl-imidazolium acetate ([EMIM]Ac) and its co-solvents with dimethylformamide (DMF) was conducted using Saccharomyces cerevisiae (D452-2). The optical density change due to the yeast cell growth, the consumption amount of monosugars (glucose, xylose), the concentration of acetate, and ethanol production yield were investigated. The co-solvent system lowered inhibition of the growth of the cells. The highest concentration of glucose (7.8 g/L) and xylose (3.6 g/L) was obtained from the enzymatic hydrolysates of the pretreated product by pure [EMIM]Ac. The initial concentration of both monosugars in the enzymatic hydrolysates was decreased with increasing fermentation time. Ethanol of Approximately 3 g/L was produced from the enzymatic hydrolysates by pure [EMIM]Ac and co-solvent with less than 50% DMF.