• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1606-1610
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• 2004

This study was. conducted to investigate the effects of $\delta$-aminolevulinic acid supplementation on growth. performance, nutrient digestibility and hematological changes in weaned pigs. Seventy five Duroc${\times}$Yorkshire${\times}$ Landrace pigs (7.21$\pm$0.02 kg average BW) were used in a 20 d growth assay. Dietary treatments included 1) NC (negative control; basal diet), 2) PC (positive control; NC diet + 0.1% Apramycin + 0.1% Oxytetracycline), 3) ALA0.l (NC diet+0.l% $\delta$-aminolevulinic acid), 4) ALA0.2 (NC diet+0.2% $\delta$-aminolevulinic acid) and 5) ALA + AB (PC diet+0.2% $\delta$-aminolevulinic acid). In entire experimental period, ADG (Average Daily Gain) was greater in pigs fed ALA + AB diet than in pigs fed NC diet (p<0.05). However, there was no significant difference in ADG for pigs fed PC, ALA0.l or ALA0.2 diets. Also, ADFI (Average Daily Feed Intake) and G/F (Gain/Feed) were not affected by treatments. For 20 d, fecal digestibilities of DM (Dry Matter) and N (Nitrogen) were higher (p<0.05) for pigs fed ALA + AB diet than for pigs fed NC and ALA0.l diets. Total protein concentration of serum was increased in ALA + AB treatment compared to NC and PC treatments (p<0.05). In iron concentration of serum, pigs fed $\delta$-aminolevulinic acid supplementation diets were greater than for pigs fed NC and PC diets (p<0.05). TIBC (Total Iron Binding Capacity) concentration of serum was increased in ALA + AB treatment compared to NC, PC and ALA0.l treatments (p<0.05). Pigs fed ALA + AB and ALA0.2 diets were higher hemoglobin (Hb) and hematocrit (HCT) concentration of blood than pigs fed NC and PC diets (p<0.05). RBC and WEC concentrations of blood were significantly higher (p<0.05) for the pigs fed ALA0.2 and ALA + AB diets than for pigs fed NC diet. Lymphocyte concentration of blood was increased in $\delta$-aminolevulinic acid supplementation treatment compared to NC treatment (p<0.05). In conclusion, $\delta$-aminolevulinic acid supplementation for weanling pigs had improved growth performance and nutrient digestibilities and had increased total protein, iron, hemoglobin and lymphocyte concentration of blood. Also, feeding for dietary $\delta$-aminolevulinic acid and antibiotic mixture supplementation may have more higher effective growth performance in weanling pigs.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.217-223
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• 2021

5-aminolevulinic acid (ALA) is a representative photosensitizer used in numerous fields including cancer diagnosis and treatment. In this study, experiments were conducted to optimize the growth of Rhodobacter sphaeroides and production of ALA through LED irradiation of various wavelengths, addition of organic acid precursors of ALA, and changes in glucose concentration. After 72 h cultivation, the 850 nm wavelength LED irradiated at the same light intensity as the incandescent lamp increased the growth of R. sphaeroides and the production of ALA about 1.5- and 1.8-fold as compared with the control, respectively (p <0.0001 and p <0.0001). As a result of culturing R. sphaeroides by irradiating an LED with a wavelength of 850 nm after adding organic acid to the final concentration of 5 mM in culture medium, the production of ALA was increased about 2.8-fold in medium supplemented with pyruvic acid compared with the control (p <0.0001). In addition, the growth of the strain and the production of ALA were increased about 2.9- and 3.4-fold in medium supplemented with 40 mM glucose compared to the control which added only 5 mM pyruvic acid, respectively (p <0.0001 and p <0.0001). The yield of ALA per cell dry mass was about 1.4 folds higher than that of the control in 20 and 40 mM glucose, respectively (p <0.001). In conclusion, the growth of R. sphaeroides and production of ALA were increased by 850 nm wavelength LED irradiation. It also optimized the growth of R. sphaeroides and production of ALA through organic acid addition and glucose concentration changes.