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http://dx.doi.org/10.3839/jabc.2021.031

Effect of 850 nm near-infrared light emitting diode irradiation on the production of 5-aminolevulinic acid in Rhodobacter sphaeroides  

Mo, SangJoon (Medical Laser Research Center, Dankook University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.3, 2021 , pp. 217-223 More about this Journal
Abstract
5-aminolevulinic acid (ALA) is a representative photosensitizer used in numerous fields including cancer diagnosis and treatment. In this study, experiments were conducted to optimize the growth of Rhodobacter sphaeroides and production of ALA through LED irradiation of various wavelengths, addition of organic acid precursors of ALA, and changes in glucose concentration. After 72 h cultivation, the 850 nm wavelength LED irradiated at the same light intensity as the incandescent lamp increased the growth of R. sphaeroides and the production of ALA about 1.5- and 1.8-fold as compared with the control, respectively (p <0.0001 and p <0.0001). As a result of culturing R. sphaeroides by irradiating an LED with a wavelength of 850 nm after adding organic acid to the final concentration of 5 mM in culture medium, the production of ALA was increased about 2.8-fold in medium supplemented with pyruvic acid compared with the control (p <0.0001). In addition, the growth of the strain and the production of ALA were increased about 2.9- and 3.4-fold in medium supplemented with 40 mM glucose compared to the control which added only 5 mM pyruvic acid, respectively (p <0.0001 and p <0.0001). The yield of ALA per cell dry mass was about 1.4 folds higher than that of the control in 20 and 40 mM glucose, respectively (p <0.001). In conclusion, the growth of R. sphaeroides and production of ALA were increased by 850 nm wavelength LED irradiation. It also optimized the growth of R. sphaeroides and production of ALA through organic acid addition and glucose concentration changes.
Keywords
5-aminolevulinic acid; Near-infrared light emitting diode; Photosensitizer; Rhodobacter sphaeroides; Photodynamic therapy;
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1 Ma G, Ikeda H, Inokuchi T, Sano K (1999) Effect of photodynamic therapy using 5-aminolevulinic acid on 4-nitroquinoline-1-oxide-induced premalignant and malignant lesions of mouse tongue. Oral Oncol 35: 120-124. doi: 10.1016/S1368-8375(98)00066-9   DOI
2 Sasaki K, Tanaka T, Nishizawa Y, Hayashi M (1990) Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor. Appl Microbiol Biotechnol 32: 727-731   DOI
3 Germaine C-B, Sistrom WR, Stainer RY (1957) Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49: 25-68   DOI
4 Kang Z, Ding W, Gong X, Liu Q, Du G, Chen J (2017) Recent advances in production of 5-aminolevulinic acid using biological strategies. World J Microbiol Biotechnol 33: 200   DOI
5 Sasaki K, Watanabe M, Nishio N (1997) Inhibition of 5-aminolevulinic acid (ALA) dehydratase by undissociated levulinic acid during ALA extracellular formation by Rhodobacter sphaeroides. Biotechnol Lett 19: 421-424   DOI
6 Nishikawa S, Watanabe K, Tanaka T, Miyachi N, Hotta Y, Murooka Y (1999) Rhodobacter sphaeroides mutants which accumulate 5-aminolevulinic acid under aerobic and dark conditions. J Biosci Bioeng 87: 798-804. doi: 10.1016/S1389-1723(99)80156-X   DOI
7 Miyachi N, Tanaka T, Nishikawa S, Takeya H, Hotta Y (1998) Preparation and chemical properties of 5-aminolevulinic acid and its derivatives. Porphyrins 2: 342-347
8 Daniell MD, Hill JS (1991) A history of photodynamic therapy. Aust N Z J Surg 61: 340-348. doi: 10.1111/j.1445-2197.1991.tb00230.x   DOI
9 Ackroyd R, Kelty C, Brown N, Reed M (2001) The history of photodetection and photodynamic therapy. Photochem Photobiol 74: 656-669. doi: 10.1562/0031-8655(2001)0740656THOPAP2.0.CO2   DOI
10 Dougherty TJ, Gomer CJ, Henderson BW, Jori G, Kessel D, Korbelik M, Moan J, Peng Q (1998) Photodynamic therapy. J Natl Cancer Inst 90: 889-905. doi: 10.1093/jnci/90.12.889   DOI
11 Huang J, Fraser ME (2016) Structural basis for the binding of succinate to succinyl-CoA synthetase. Acta Crystallogr D Struct Biol 72(Pt 8): 912-921. doi: 10.1107/S2059798316010044   DOI
12 Yang D-S, Park M-W, Lim, MJ Kim SJ, Shin Y, Park CS, Hyun Y, Kang D-K (2009) Optimizing the production of 5-aminolevulinic acid by recombinant Escherichia coli containing the Rhodobacter capsulatus hemA Gene. Kor J Microbiol Biotechnol 37: 153-159
13 Bourget CM (2008) An introduction to light-emitting diodes. Hort Sci 43: 1944-1946. doi: 10.21273/HORTSCI.43.7.1944   DOI
14 Carvalho AP, Silva S O, Baptista JM, Malcata FX (2011) Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Appl Microbiol Biotechnol 89: 1275-1288   DOI
15 Sistrom WR (1962) The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroids. J Gen Microbiol 28: 607-616. doi: 10.1099/00221287-28-4-607   DOI
16 Lou J-W, Zhu L, Wu M-b, Yang L-r, Lin J-p, Cen P-l (2014) High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties. J Zhejiang Univ Sci B 15: 491-499   DOI
17 Kim YU, Kim KS (1981) Studies on the isolation and the application of photosynthetic bacteria. J Kor Agr Chem Soci 24: 132-138
18 Hwang SY, Choi KM, Lim WJ, Hong BS, Cho HY, Yang HC (1992) Isolation of Rhodocyclus gelatinosus KUP-74 and characterization of δ-aminolevulinic acid production. J Korean Agric Chem Soc 35: 210-217
19 Kim D, Chang SY, Ahn JC (2008) Effect of growth improvement in photosynthetic bacteria as a function of 880 nm light emitting diode luminosity. J Exp Biomed Sci 14: 91-96
20 Mauzerall D, Granick S (1956) The occurrence and determination of delta-aminolevulinic acid and porphobilinogen in urine. J Biol Chem 219: 435-446   DOI
21 Brasen C, Schonheit P (2001) Mechanisms of acetate formation and acetate activation in halophilic archaea. Arch Microbiol 175: 360-368   DOI
22 Tangprasittipap A, Prasertsan P, Choorit W, Sasaki K (2007) Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides. Biotechnol Lett 29(5): 773-778   DOI
23 Bertling K, Hurse TJ, Kappler U, Rakic AD (2006) Lasers-an effective artificial source of radiation for the cultivation of anoxygenic photosynthetic bacteria. Biotechnol Bioeng 94(2): 337-345. doi: 10.1002/bit.20881   DOI
24 Yu T-H, Yi Y-C, Shih I-T, Ng I-S (2020) Enhanced 5-aminolevulinic acid production by co-expression of codon-optimized hemA Gene with chaperone in genetic engineered Escherichia coli. Appl Biochem Biotechnol 191(1): 299-312   DOI
25 Kim JK, Lee BK (2000) Mass production of Rhodopseudomonas palustris as diet for aquaculture. Aqua Eng 23: 281-293. doi: 10.1016/S0144-8609(00)00057-1   DOI
26 Sasaki K, Watanabe M, Tanaka T, Tanaka T (2002) Biosynthesis, biotechnological production and applications of 5-aminolevulinic acid. Appl Microbiol Biotechnol 58: 23-29   DOI
27 Neidle EL, Kaplan S (1993) Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes. J Bacteriol 175(8): 2292-2303. doi: 10.1128/jb.175.8.2292-2303.1993   DOI
28 Kamiyama H, Hotta Y, Tanaka T, Nishikawa S, Sasaki K (2000) Production of 5-aminolevulinic acid by a mutant strain of a photosynthetic bacteria. Seibutu-Kougaku 78: 48-55
29 Zhang SJ, Zhang ZX (2004) 5-aminolevulinic acid-based photodynamic therapy in leukemia cell HL60. Photochem Photobiol 79: 545-550. doi: 10.1111/j.1751-1097.2004.tb01274.x   DOI