• 한국작물학회지
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• 제42권6호
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• pp.739-747
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• 1997

본 실험은 lipoxygenase-1 효소 검정을 위한 관련요인 구명과 새로운 검정방법의 활용성을 확립함으로써 무두취 콩품종 육성사업에 도움이 될 수 있는 자료를 얻고저 수행되었다. 1. 기질용액의 완충제로서 Tris나 potassium borate가 효과적이었고 그중 0.1M potassium brate가 lipoxygenase-1의 검정에 있어 가장 효과적이었으며 적정 pH의 범위는 pH 8.5~9.0 이었다. 2. 기질인 linoleic acid의 적정 농도는 2mM이었고 기질용액은 4$^{\circ}C$ 냉장고에서 10일 정도 실온상태에서 4일 정도까지 안전하게 보관하면서 사용할 수 있었다. 3. 콩 종실의 lipoxygenase-1 유무 여부의 확인을 위한 가장 적당한 검정방법은 종피와 배가 제거되고 얇게 자른 반립의 종자를 1.5ml크기의 plastic tube에 넣고 기질용액(0.1M potassium borate (pH 9.0), 0.1% Tween-20, 2mM linoleic acid)을 1ml 첨가해 주고 15분 경과 후 포화된 KI 용액이 5% 함유된 15% acetic acid와 1% 전분용액을 각각 100$\mu$l씩 첨가한 뒤 4시간 후에 상층액의 발색반응을 관찰하는 것으로 판명 되었다. 4. Lipoxygenase-1이 존재하는 종실의 상층액에서는 보라색 발색반응을 보였고 lipoxygenase-1이 결핍된 종실의 상층액에서는 우유빛이 나타났으며 lipoxygenase-2와 3의 간섭효과는 없었다. 보라색 발색은 4시간부터 24시간까지 안정하였고 발색용액(KI와 starch)첨가한 뒤에는 plastic tube를 흔들림없이 유지시켜야 했다.

• 분석과학
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• 제34권4호
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• pp.153-159
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• 2021

In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 ㎍/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 ㎍/mL and 0.08-0.26 ㎍/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권4호
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• pp.442-446
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• 2010

본 논문에서는 대량의 사진 데이터베이스에 대한 효과적인 사진 검색을 위하여 브라우징을 지원하는 인덱싱 방법을 제안하였다. 제안한 방법은 대표적인 거리 공간 인덱싱 방법인 M-트리를 기본 구조로 하였다. 그러나 M-트리는 pruning을 통한 검색의 효율성에 초점을 맞추었으며 브라우징을 직접적으로 고려하지 않는다. 따라서 본 논문에서는 M-트리의 노드 선택 방법, 노드 분할 방법 및 노드 분할 조건을 브라우징에도 적합하도록 변경하였다. 제안한 방법을 적용하여 실험한 결과 노드 응집도와 클러스터링 정확도에서 각각 2배와 1.5배의 향상을 나타내었으며, 검색 성능도 약 2배정도 빨라지는 효과를 확인하였다.

• Transactions on Electrical and Electronic Materials
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• 제8권4호
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• pp.182-187
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• 2007

The use of the pulsed electro acoustic (PEA) method allowed us to perform the direct observations of spatio-temporal charge distributions in Electric double layer capacitors (EDLCs) based on polarizable nanoporous carbonaceous electrode. The negative charge density became the maximum, about $205C/m^3$ at the region where was near to collector layer in EDLCs for case $V_{DC}=2.5V$, while the positively charged density became the maximum, about $61.1C/m^3$ at the region where it was located around the cathode layer. The performance of the best sample was found to be better in terms of the charge density (Cs) and specific energy ($E_s$) with a maximum value of ${\sim}8.4F/g$ and 26 Wh/kg. The $C_s$ obtained from the PEA method agreed well with that from the energy conversion method. The PEA measurement used here is a very useful method to quantitively investigates the spatio-temporal charge distribution in EDLCs.

• 대한가정학회지
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• 제35권4호
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• pp.159-172
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• 1997

The purpose of this study was to investigate the slacks pattern making on the movement -fitness of the hip region. The experimental items were divided into the 5 lower limb movements(M1-M5) and the 12 revision pattern constructions of slacks. This study was done by clothing pressure test sensory evaluation test and the difference shape-transformation of wearing-slacks. The summarized finding resulted from experiments and investigation are suggested as follows: First by the clothing pressure tested by lower limb movements the clothing pressure score of the rabbit leap movement(M3) was the highest of all lower limb movements and the order of it in the another movement was the sit on knee(M4) the sit on chair(M2) the noble sitting(M5) from the highest to the lowest. And in comparision of clothing pressure tested by revision pattern to cover the extend of crotch-length 20。 inclined back-line construction method (CA4) showed the lowest. And in comparision of clothing pressure tested by revision pattern to cover the extend of crotch-length 20。 inclined back-line construction method (CA4) showed the lowest. And also for a role to cover hip circumference extendign CC4(1/5 hip.1cm longrightarrow linked back -line) method showed the lowest. Second by the sensory evaluation test based on the movement and revision patterns it was found that the functional factor score of the rabbit leap movement was the lowest in all movement however the score of revision patterns were higher than basic pattern. Third by the test to show difference in the shape-transformation of wearing-slacks on chair(M2) also need to be analyzed. The ration of the shape-transformation of the knee region showed the least value in the increased patterns of inclined angle of back-line(CA) and the differential methods of back-line inclined pattern making. But that of the hem-line did not show remarkable difference.

• 전력전자학회논문지
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• 제17권4호
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• pp.359-367
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• 2012

In this paper, the input split type series-parallel hybrid electric vehicle (SPHEV) is established and the interpretation of the dynamic characteristics in four kinds of HEV modes, such as electric vehicle mode, engine mode, hybrid mode, and regeneration mode, is described. For this research, the forward-facing approach simulation method is chosen, which is useful for vehicle dynamic analysis. The rating of each powertrain component is designed based on energy-based concept and electrical peaking hybrid (ELPH) method. Finally, the designed powertrain is evaluated with the developed PSiM simulator and simulation results are shown.

• 소성∙가공
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• 제17권4호
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• pp.257-262
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• 2008

The control of wire temperature is very important in the fine wire drawing process. The wire speed should be increased, and the wire temperature should be dropped as much as possible. Up to now, the process design of wire drawing process depends on the experiences of experts. In this study, a wire drawing process design method was proposed to increase the productivity. The proposed method of this study includes the pass schedule and the design of a multi pass wire drawing machine. A pass schedule was performed based on the calculation of the wire temperature. Also, a new multi pass wire drawing machine was manufactured to apply the designed pass schedule. Through the wire drawing experiment, the effectiveness of the proposed process design method was evaluated. The final drawing speed was increased from 1,100m/min to 2,000m/min without deterioration of final drawn wire.

• 대한화학회지
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• 제54권3호
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• pp.283-286
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• 2010

지르코늄의 부유-분광 광도의 측정에 대한 감도가 좋고 재현성 있는 방법이 기록되었다. 그 방법은 지르코늄과 자일렌오렌지(XO)착물 형성에 기초하였고 그것은 격렬한 진탕에 의해 수용액 상과 n-헥산의 표면에 떠있게 된다. 수용액과 n-헥산을 폐기함에 따라 분리된 깔대기의 벽에 있던 흡착된 착물은 작은 부피의 메탄올 용액으로 해리되어진다. 그리고 그것의 흡광도는 429 nm에서 측정되었다. PH, HCl, XO의 농도, n-헥산 부유 용매의 부피, 지속시간과 진탕시간과 같은 다른 변수의 효과가 연구되었다. 보정곡선은 0.9991의 상관계수를 갖는 지르코늄의 7 ~ 120 ng/mL의 범위에서 선형이었다. 검출한계(LOD)는 58 ng/mL이었다. 지르코늄의 50과 110 ng/mL 의 7개의 분할척정에 대한 상대표준편차(RSD)는 4.4와 3.0%였다. 이 방법은 물샘플에서 지르코늄의 측정에 성공적으로 적용되었다.

• 대한화학회지
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• 제57권5호
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• pp.568-573
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• 2013

A selective and sensitive method for simultaneous determination of copper in blood by adsorptive differential pulse cathodic stripping voltammetry is presented. The procedure involves an adsorptive accumulation of Cu(II)-ETSC (4- ethyl-3-thiosemicarbazide) on a hanging mercury drop electrode, followed by a stripping voltammetry measurement of reduction current of adsorbed complex at about -715 mV. The optimum conditions for the analysis of copper (II) ion are : pH 10.3, concentration of 4-ethyl-3-thiosemicarbazide $3.25{\times}10^{-6}$ M and an accumulation potential of -100 mV. The peak current is proportional to the concentration of copper over the range 0.003-125 ng/mL with a detection limit of 0.001 ng/mL and an accumulation time of 60 s. Moreover, with the use of the proposed method, there is a considerable improvement in the detection limit, the linear dynamic range and the deposition time, compared with the methods of adsorptive stripping voltammetry for the determination of copper. The developed method was validated by analysis of whole blood certified reference materials.

• 한국식품영양과학회지
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• 제24권1호
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• pp.133-140
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• 1995

This investigation was undertaken for the purpose of studying the changes of pectic substances in alcohol insoluble solids(AIS) extracted from cucumbers pickled in salt. The preparation methods were brining the cucumbers in 10% NaCl solution (bolied, method A), 20% NaCl solution(bolied, method B) and 20% NaCl solution(cool to $25^{\circ}C$ after boiling, method C). The composition ratio of hot water soluble pectin(HWSP) in AIS decreased during brining, while that of 0.4% sodium hexametaphosphate soluble pectin(NaSP) increased remarkably. The contents of Ca and Mg in AIS increased regardless of preparation methods used. By DEAE-cellulose chromatography, HWSP from fresh and brined cucumbers were divided into six fracitons. 0.2M peak was the main fraction in fresh cucumber, whereas 0.05M and 0.2M peak were the main fraction in pickled cucumbers. On the other hands, NaSP from fresh and brined cucumbers were divided into five fractions and 0.4M peak was the main fraction. However, the composition ratios of galacturonic acid and neutral sugar of 0.4M fraction in brined cucumbers were higher than those of fresh cucumber.