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Initial Control of Microorganism in Kimchi by the Modified Preparation Method of Seasoning Mixture and the Pretreatment of Electrolyzed Acid-Water (전해산화수 전처리 및 양념류 제조방법에 따른 김치의 초기 미생물 제어)

  • Park, Kee-Jai;Jung, Sung-Won;Park, Byoung-In;Kim, Young-Ho;Jeong, Jin-Woong
    • Korean Journal of Food Science and Technology
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    • v.28 no.6
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    • pp.1104-1110
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    • 1996
  • To lessen the initial level of microorganism in kimchi, the preparation method of seasoning mixture was modified and electrolyzed acid-water was substitute for washing water and brine water. Changes in the microbial counts of the red pepper powder-garlic mixture prepared with red pepper powder, garlic and 4.8% (w/w) water (tap water and electrolyzed acid-water) of manufactured kimchi showed that coliform count and E. coli count were reduced to 93% and 98%, of the initial level in the tap water added red pepper powder-garlic mixture and 97% and 99% in the electrolzed acid-water added mixture after 6 hours of mix. After 24 hours of mix, no E. coli was recovered in borth mixtures. Microbial levels were revealed as $2.4{\times}10^5\;CFU/g$ for total count, $2.3{\times}10^5\;CFU/g$ for Lactobacillus count. $1.0{\times}10^2{\;}CFU/g$ of coliform count and $10^1\;CFU/g$ of E. coli count in the seasoning mixture prepared by the general method, and $4.2{\times}10^5\;CFU/g$ of total count, $4.0{\times}10^5CFU/g$ of Lactobacillus count, $1.0{\times}10^3\;CFU/g$ of coliorm count and $4.0{\times}10^2\;CFU/g$ of E. coli count in the mixture propared by the modified method. During fermentation at $10^{\circ}C$, the levels of total and Lactobacillus counts in kimchi prepared by the modified method were significantly lower than those of kimchi were recovered in kimchi prepared by the modified method, whereas there were at the level of $10^1\;CFU/g$ in kimchi prepared by the general method. The pH and acidity of kimchi prepared by the modified method were 4.66 and 0.54%, respectively, whereas those in kimchi prepared b the general method were 4.51 and 0.70%, respectively. But after 14 days of fermentation significant differences were not observed in the changes of microbial, pH and acidity for both kimchi.

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A Study on Preparation of Water in Oil in Water (W1/O/W2) Emulsion Containing Titrated Extract of Centella asiatica (센텔라 아시아티카 정량추출물을 함유한 Water in Oil in Water (W1/O/W2) 에멀젼 제조에 관한 연구)

  • Seo, Dong Hoan;Lee, Hong Seon;Yoon, Jong Hyuk;Kim, Youn Joon;Byun, Sang Yo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.303-313
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    • 2015
  • Titrated extract of Centella asiatica (TECA), which is poorly soluble in water is well known for wound healing and anti wrinkle agent. This study was conducted to find the optimum condition for the preparation of water in oil in water ($W_1/O/W_2$) emulsion containing TECA. Solubility of TECA were measured by UV spectrophotometer. 2.55 g of TECA was dissolved in solution composed of dipropylene glycol (40.0 g), ethanol (20.0 g), and water (10.0 g). Factors affecting stability of the emulsions ($W_1/O$, $W_1/O/W_2$) was investigated. The optimum conditions for the preparation of $W_1/O$ emulsion was composed of dipropylene glycol : ethanol : water : TECA in a weight ratio of 40.0 : 20.0 : 10.0 : 2.5 for water phase and squalane : cetyl PEG/PPG-10/1 dimethicone : cetearyl alcohol in a weight ratio of 22.5 : 4.0 : 2.5 for oil phase. The optimum conditions for the preparation of $W_1/O/W_2$ multiple emulsion was composed of water : $W_1/O$ emulsion : polysorbate 80 : carbomer : triethanolamine in a weight ratio of 55.8 : 40.0 : 4.0 : 0.1 : 0.1.

Total Phenols, Flavonoid Contents, and Antioxidant Activity of Spirodela polyrhiza Extracts (부평초 추출물의 페놀 및 플라보노이드 함량 분석 및 항산화 활성)

  • Song, Won-Yeong;Choi, Jeong-Hwa
    • Journal of Life Science
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    • v.27 no.2
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    • pp.180-186
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    • 2017
  • We investigated the antioxidant activities of water and ethanol extracts from Spirodela polyrhiza (SP) through in vitro assays. The total phenolic contents of SP water and ethanol extracts were 52.75-293.4 and 60.12-398.4 mg/g, respectively. The total flavonoid content of SP ethanol extract (38.25-159.4 mg/g) was higher than that of SP water extract (38.25-67.75 mg/g). The water and ethanol extracts from SP scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2'-azino-di-2-ethyl-benzothia-zoline sulfonate (ABTS) radical in a dose-dependent manner in the concentration range of $100-2,500{\mu}g/ml$. The DPPH radical scavenging activity of the SP ethanol extract (2.87%-59.5%) was higher than that of the water extract (4.12%-81.52%). The $IC_{50}s$ of the DPPH radical scavenging activity of water and ethanol extracts were 2,100 and $1,034{\mu}g/ml$ respectively. The ABTS radical scavenging activities of SP water and ethanol extracts were 8.30%-83.16% and 13.11%-8.34% respectively. The $IC_{50}s$ of the ABTS radical scavenging activity of SP water and ethanol extracts were 798.7 and $457.1{\mu}g/ml$, respectively. The reducing power activities of SP water and ethanol extracts were 0.055-1.122 and 0.140-1.428, respectively ($500-4,000{\mu}g/ml$). The soybean lipoxygenase (SLO) radical scavenging activities of SP water and ethanol extracts were 157.7%-168.0% and 148.0%-169.4%, respectively. These results suggest that the water and ethanol extracts of SP may be useful as a potential antioxidant.

Algae removal by Loess and coagulant (황토와 응집제에 의한 조류 제거)

  • 양상용;구연봉;최지혁;이인선;신찬기;유재근
    • Journal of environmental and Sanitary engineering
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    • v.12 no.3
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    • pp.127-130
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    • 1997
  • The removal of algae was conducted by loess, aluminum sulfate and PAC on a laboratory scale. The loess was consists of organic matter 1.4%, T-N $289{\mu}g/g$, T-P $17{\mu}g/g$, $Al841.2{\mu} g/g$, Fe $592.7{\mu}g/g$, Ca $10.6{\mu}g/g$, Mg $85.5{\mu}g/g$ and Mn $6.6{\mu}g/g$. Test water was dominated by Microcystis aeruginosa. When test water was mixed with 0.01, 0.05. and 0.1 g/$\ell$ of the loess in 5 minutes, after settled in 1 hour, the removal of chl-a was 2, 22, and 36% respectively. The removal of chl-a was 69%, 70% in pH 4.5 and 9.0 and above 92% between pH 5.0 and 8.0 after the $2mg/{\ell}$ of aluminum sulfate was added. When the $2mg/{\ell}$ of PAC and $0.05g/{\ell}$ of loess were added to test water together, the removal of chl-a was 95-99% in pH 4.0-8.0, 60% in pH 9.0, and 18% in pH 10.0. The removal of chl-a was higher when loess, aluminum sulfate and PAC was used together than used alone.

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The experimental study for high ammonia nitrogen removal using Bardenpho process with Methanol addition (메탄올주입에 의한 Bardenpho공법에서의 고농도 암모니아성 질소 제거에 관한 실험적 연구)

  • Lee, Byonghi
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.2
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    • pp.34-40
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    • 1999
  • Aerobic night-soil treatment effluent containing high concentration of ammonia nitrogen was treated to remove nitrogen using Bardenpho process with Methanol addition. The objective of this study was to investigate the feasibility of complete nitrogen removal at three different HRTs such as 6.25d, 5d, and 3.75d, respectively. At each HRT, the nitrogen removal efficiencies are 92%, 99% and 97% and the required amount of methanol are 3.05gMeOH/gN, 2.75gMeOH/gN, and 3.38gMeOH/gN, respectively. Specific nitrification rates are decreased proportional to HRT and are $0.022gNH_4^+-N/g\;MLVSS{\cdot}day$, $0.0332gNH_4^+-N/g\;MLVSS{\cdot}day$ and $0.051gNH_4^+-N/g\;MLVSS{\cdot}day$ and specific denitification rate are decreased proportional to HRT and are $0.0210g\;N/gMLVSS{\cdot}day$, $0.0330g\;N/gMLVSS{\cdot}day$ and $0.0525g\;N/gMLVSS{\cdot}day$, respectively.

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The Effect of Wood Extract as a Water-Soluble Fertilizer in the Growth of Lactuca sativa

  • JUNG, Ji Young;HA, Si Young;YANG, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.4
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    • pp.384-393
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    • 2021
  • Recently, due to environmental and toxicity issues, there has been increasing attention on research regarding natural products that can reduce the use of chemical fertilizers. Wood extracts derived from the biorefining process contain various fertilizer ingredients. HPLC analysis revealed that wood extract contains approximately 5.2% hemicellulosic sugar. The growth of lettuce (Lactuca sativa) upon treatment with wood extract (extract obtained from steam-exploded pine) or water-soluble fertilizers containing different nutrients was analyzed in this study. After two weeks, the growth characteristics of lettuce as affected by wood extract or water-soluble fertilizers were significantly different. The effect of water-soluble fertilizers containing ascorbic acid, magnesium sulfate, citric acid, potassium nitrate, amino acids, or seaweed extract was less desirable than that of wood extracts regarding plant height (18.6 cm), number of leaves (10), leaf length (14.1 cm), shoot fresh wight (9.8 g/plant), root fresh weight (0.8 g/plant) and shoot dry weight (0.6 g/plant). The plant height, number of leaves, leaf length, shoot fresh wight, root fresh weight, shoot dry weight of water-soluble fertilizers containing wood extract were significantly different compared to the control (plant height :13.5 cm, number of leaves : 7, leaf length : 9.4 cm, shoot fresh wight : 5.3 g/plant, root fresh weight : 0.7 g/plant, shoot dry weight : 0.4 g/plant, root dry weight : 0.07 g/plant). From these results, it was concluded that wood extract can be used as a potential water-soluble fertilizer to increase the yield of leafy vegetables.

Water Requirement of Twist Peppers in Greenhouse (온실 재배 꽈리고추의 필요수량)

  • 윤용철
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.42 no.4
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    • pp.59-66
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    • 2000
  • This study was carried out to investigated the water requirement of twisted sweet peppers which are cultivated in a green house. The meteorological conditions during the experiment period was close to that of normal year except the temperature and relative humidity. The growth status was improved with the increased saturation ratio. The range of the variation of daily water requirement were very large. The peak consumption occurred in the early August. And the higher saturation ratio resulted in higher water requirement. The total water requirement were about 57.180g/d/plant for pot with 100% (P100) of saturation , about 38.700g/d/plant for pot with 80%(P80) of saturation , about 23,720g/d/plant for pot with 60%(P60) of saturation, and about 53, 390g/d/plant for field cultivation in the green house, respectively. The water requirement was correlated with average ambient temperature and growing status, while no significant correlation were found between water requirement and minimum relative humidity or intensity of solar radiation. And the higher correlation was shown as the saturation ratio was increased. The transpiration coefficients of twisted sweet pepper were 378.0g/g for field cultivation in the green house, 363.3g/g for P100, 338.7g for P80 which was the smallest among pot cultivation , and 472.1g/g for P60 , respectively.

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Acute and Chronic Toxicity of heavy Metals to Daphnia magna (물벼룩(Daphnia magna)에 대한 중금속의 급성 및 만성 독성)

  • Jeong, Jae-Won;Cha, Mi-Seon;Jo, Sun-Ja;Lee, Sang-Jun
    • Journal of Environmental Science International
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    • v.10 no.4
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    • pp.293-298
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    • 2001
  • The toxicity values heavy metals were evaluated by immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange or water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3~1.4 and DO also increased. 2) The $E_iC_{50}$ values of immobilization to Daphnia magna in artificial fresh water were $0.030mg/{\ell}(Cu),\;0.054mg/{\ell}(Cd),\;0.12mg/{\ell};(Cr),\;0.74mg/{\ell}(Pb),\;3.4mg/ {\ell}(As)$ and the $NOE_iC$ values were $0.010mg/{\ell}(Cu),\;0.018mg/{\ell}(Cd),\;0.010mg/{\ell}(Cr),\;0.10mg/{\ell}(Pb),\;and\;$1.8mg/{\ell}(As)$. 3) The $E_rC_{50}$ values of reproductive impairment to Daphnia magna were $13.8\mu\textrm{g}/{\ell}(Cu),\;2.9\mu\textrm{g}/{\ell}(Cd),\;15.5\mu\textrm{g}/{\ell}(Cr),\;61.7\mu\textrm{g}/{\ell}(Pb),\;759\mu\textrm{g}/{\ell}(As)$, and $NOE_rC$ values were $0.95\mu\textrm{g}/{\ell}(Cu),\;$0.54\mu\textrm{g}/{\ell}(Cd),\;1.2\mu\textrm{g}/{\ell}(Cd),\;$7.4\mu\textrm{g}/{\ell}(Pb),\;110mu\textrm{g}/{\ell}(As)$. The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of marterials.

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Desalination of geothermal water by membrane distillation

  • Gryta, M.;Palczynski, M.
    • Membrane and Water Treatment
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    • v.2 no.3
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    • pp.147-158
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    • 2011
  • Membrane distillation process was used for desalination of hot (333 K) geothermal water, which was applied in the plant producing heating water. The investigated water contained 120 g salts/$dm^3$, mainly NaCl. The mineral composition was studied using an ion chromatography method. The obtained rejection of solutes was closed to 100%, but the small amounts of $NH_3$ also diffused through the membrane together with water vapour. However, the composition of obtained distillate allowed to use it as a makeup water in the heating water system. The geothermal water under study was concentrated from 120 to 286 g NaCl/$dm^3$. This increase in the solution concentration caused the permeate flux decline by a 10-20%. The geothermal water contained sulphates, which was subjected to two-fold concentration to achieve the concentration 2.4-2.6 g $SO{_4}{^{2-}}/dm^3$ and the sulphates then crystallized in the form of calcium sulphate. As a results, an intensive membranes scaling and the permeate flux decline was observed. The XRD analysis indicated that beside the gypsum also the NaCl crystallites were deposited on the membrane surfaces. The fresh geothermal water dissolved the mixed $CaSO_4$ and NaCl deposit from the membrane surface. This property can be utilized for self-cleaning of MD modules. Using a batch feeding of MD installation, the concentration of geothermal water was carried out over 800 h, without significant performance losses.

Evaluation of Adsorption Characteristics of Radioactive Iodine (I-131) for Various Materials of Granular Activated Carbon (GAC) (입상활성탄 재질별 방사성 핵종(I-131) 흡착 특성 평가)

  • Park, Hong-Ki;Son, Hee-Jong;Yeom, Hoon-Sik;Kim, Young-Jin;Choi, Jin-Taek;Ryu, Dong-Choon
    • Journal of Environmental Science International
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    • v.24 no.9
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    • pp.1123-1129
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    • 2015
  • This research was performed by means of several different virgin granular activated carbons (GAC) made of each coal, coconut and wood, and the GACs were investigated for an adsorption performance of iodine-131 in a continuous adsorption column. Breakthrough behavior was investigated that the breakthrough points of the virgin two coals-, coconut- and wood-based GACs were observed as bed volume (BV) 7080, BV 5640, BV 5064 and BV 3192, respectively. The experimental results of adsorption capacity (X/M) for iodine-127 showed that two coal- based GACs were highest (208.6 and $139.1{\mu}g/g$), the coconut-based GAC was intermediate ($86.5{\mu}g/g$) and the wood-based GAC was lowest ($54.5{\mu}g/g$). The X/M of the coal-based GACs was 2~4 times higher than the X/M of the coconut-based and wood-based GACs.