• Title/Summary/Keyword: 4D-Var

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Cytotoxic and Anti-oxidant Constituents from the Aerial Parts of Aruncus dioicus var. kamtschaticus

  • Zhao, Bing Tian;Jeong, Su Yang;Vu, Viet Dung;Min, Byung Sun;Kim, Young Ho;Woo, Mi Hee
    • Natural Product Sciences
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    • v.19 no.1
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    • pp.66-70
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    • 2013
  • Ten compounds (1 - 10), palmitic acid (1), 10-nonacosanol (2), pentacosan-1-ol (3), phytol (4), ${\beta}$-sitosterol (5), ${\beta}$-sitosterol-3-O-${\beta}$-D-glucopyranoside (6), 2,4-dihydroxycinnamic acid (7), hyperoside (8), uridine (9) and adenosine (10), were isolated from the n-hexane and EtOAc-soluble fractions of the aerial parts of A. dioicus var. kamtschaticus (Rosaceae). The structures of these compounds were elucidated on the basis of spectroscopic evidence. All compounds (1 - 10) were isolated for the first time from this plant. Cytotoxicity of 1 - 10 against Jurkat T (T-lymphocytic leukemia cells), HeLa (Human cervical epitheloid carcinoma cells), MCF-7 (Human breast cancer cells), and HL-60 (Human promyelocytic leukemia cells) cell lines was measured. Compound 6 showed good cytotoxicity against HL-60 cell line with $IC_{50}$ value of 8.13 ${\mu}g/mL$. In addition, compounds 7 and 8 exhibited antioxidant activity with $IC_{50}$ values of 16.30 and 12.42 ${\mu}g/mL$, respectively.

Analysis of Marker Components of Fermented Opuntia ficus-indica var. saboten Stem Extracts (유산균 발효에 의한 손바닥선인장 줄기추출물의 지표물질 함량 변화 분석)

  • Shin, Dong Won;Lee, Sang Ho;Lee, Soyeon;Han, Eun Hye
    • Analytical Science and Technology
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    • v.31 no.6
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    • pp.219-224
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    • 2018
  • The fruit and stem of Opuntia ficus-indica var. aboten (OFS), a native plant of Jeju Island, are considered a safe food source. Moreover, stem extracts have been previously reported to possess a variety of biological effects (e.g. anti-inflammatory and anti-oxidant, including the ability to partially ameliorate cognitive impairment), suggesting that this plant may have utility as a functional food. The present study investigated whether fermentation by lactic acid bacteria enhances the biological effects of OFS extracts. The acetylcholinesterase (AChE) inhibitory activity of fermented or non-fermented OFS extracts was evaluated, and the content of marker components dihydrokaempferol (DHK) and quercetin-3-methyl ether (3-MeQ) was analyzed using high-performance liquid chromatography. Fermented (relative to non-fermented) OFS extracts exhibited improved AChE inhibitory activity ($IC_{50}=28.35 mg/mL$), with AChE inhibitory activity resulting from fermentation by L. plantarum ($IC_{50}=12.56mg/mL$) exceeding that resulting from fermentation by L. fermentum ($IC_{50}=17.71mg/mL$). Furthermore, fermented (relative to non-fermented) OFS extracts exhibited a 16.7 % increase in DHK content, and 3-MeQ content of OFS extracts fermented by L. plantarum and L. fermentum increased by 28.6 % and 21.4 %, respectively. Therefore, OFS stem extract AChE inhibitory activity, as well as DHK and 3-MeQ content, was enhanced by fermentation with Lactobacillus spp. This suggests that fermented OFS extracts may contribute to prevention or improvement of cognitive impairment. These data are anticipated to be useful in the development of enhanced-efficacy OFS products.

Physiological Response of Young Seedlings from Five Accessions of Diospyros L. under Salinity Stress

  • Wei, Ping;Yang, Yong;Fang, Ming;Wang, Fei;Chen, Hejie
    • Horticultural Science & Technology
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    • v.34 no.4
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    • pp.564-577
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    • 2016
  • Salinity stress limits plant cultivation in many areas worldwide; however, persimmon (Diospyros spp.) has high tolerance to salt. Five accessions of Diospyros [three of Diospyros lotus (accession numbers 824, 846, and 847); one of Diospyros kaki var. sylvestris (869); and one of Diospyros virginiana (844)] were chosen for analysis of salinity stress. We compared the effects of salt stress on plant growth, relative water content (RWC), malondialdehyde (MDA), electrolyte leakage (EL), hydrogen peroxide content ($H_2O_2$), and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT; peroxidase, POD; and ascorbate peroxidase, APX) in leaves of healthy potted seedlings from each of the five accessions after salt treatment for 25 days. Salt stress affected the growth of plants in all five accessions, with all three D. lotus accessions showing the most severe effect. Salt stress increased membrane lipid peroxidation in all accessions, but a stronger increase was observed in the three D. lotus accessions. Moreover, accumulation of $H_2O_2$ was faster in salt-sensitive D. lotus compared to salt-tolerant D. virginiana 844. The activities of all antioxidant enzymes increased in D. virginiana 844 and in D. kaki var. sylvestris 869; the activities of SOD, CAT, and APX were at similar levels in D. virginiana 844 and D. kaki var. sylvestris 869, but POD activity was stimulated to a greater extent in D. virginiana 844. The activities of all antioxidant enzymes (except POD) decreased in D. lotus 824 and increased (except for SOD) in D.lotus 846. The activities of SOD and APX decreased in D. lotus 847, whereas POD and CAT activities both increased. Relative water content decreased significantly in D. lotus. No significant changes in lipid peroxidation or relevant antioxidant parameters were detected in any of the accessions in controls treated with 0.0% NaCl. D. virginiana 844 had higher antioxidant capacity in response to salinity compared to other persimmon rootstocks. These results indicate that changes of these key physiological variables are related to salinity resistance in different accessions of persimmon.

Studies on the Cracking of Nitrocellulose Clear Lacquer Coasted Films on Solid Woods of Quercus acutissima and Betula platyphylla var. japonica (상수리나무와 자작나무 소재(素材)에 대한 Nitrocellulose Clear Lacquer 도막(塗膜)의 할열(割裂)에 관(關)한 연구(硏究))

  • Kim, Hyun-Joong;Lee, Phil-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.17 no.1
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    • pp.55-68
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    • 1989
  • This experiment was carried out to investigate the crack of coated Nitrocellulose Lacquer on flatand edge-grained boards of Quercus acutissima, ring-porous wood, and Betula platyphylla var. japonica, diffuse-porous wood, by variations of moisture contents at 7, 13 and 21%. Cold check system was used as an accelerating method for crack development, in which one cycle of the system consisted of 10 replications of each unit cycle, $60^{\circ}C$ for 4hr followdd by $-20^{\circ}C$ for 4hr. The analysis of Nitrocellulose Lacquer characteristics was made by means of water permeation measurement, F.T.I.R. spectroscopy, N.M.R. spectroscopy, gel permeation chromatography, gas chromatography, and D.S.C. The results obtained were as follows: 1. The number of cracks increased with the increasing moisture content of board. 2. The crack of coated film on flat-grained board was fewer in number than on edge-grained board. 3. The crack occurred in Quercus acutissima was more numerous on edge-grained board but less frequent on flat-grained board compared with that in Betula platyphylla var. japonica, respectively. 4. The cold crack vertically developed to the grain both in Quercus acutissima and Betula platyphylla var. japonica. 5. Water permeability in intermediate coated film was lower than in under and top coated film, but the difference was not confirmed between under and top coated film.

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Antihepatotoxic effect of Heat-treated Allium victorialis var. platyphyllum in $CCl_4-induced$ Rats and the Gas Chromatographic Analysis of Volatile Sulfur Substances

  • Park, Hee-Juhn;Jung, Hyun-Ju;Lim, Sang-Cheol;Jung, Won-Tae;Kim, Won-Bae;Park, Kwang-Kyun;Lee, Jin-Ha;Choi, Jong-Won
    • Natural Product Sciences
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    • v.11 no.2
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    • pp.67-74
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    • 2005
  • The ethanolic extracts of the leaves and bulbs of Allium victorialis var. platyphyllum (Liliaceae) collected from Daegwallyoung (D) and Ullung Island (U) in Korea were obtained using three different extracting methods. The first extracts, DL-1 DB-1, UL-1 and UB-1, were obtained from leaves (L) and bulbs (B) dried at $90^{\circ}C$, respectively, and the second extracts, DL-2, DB-2, UL-2 and UB-2, were obtained by extracting the leaves and bulbs of fresh plant parts. The third extracts DL-3, DB-3, UL-3 and UB-3 were obtained by incubating leaves and bulbs at $36^{\circ}C$. The six extracts obtained from A. victorialis var. platyphyllum at Daegwanllyoung (cultivated site) were orally administered to examine for a possible antihepatotoxic effect in $CCl_4-induced$ rats. DL-1 exhibited the most pronounced effect. The extracts inhibited serum ALT, AST, SDH, ${\gamma}-GT$, ALP and LDH activities elevated by $CCl_4$ injection and attenuated decreased glutathione S-transferase, glutatione reductase and ${\gamma}-glutamylcysteine$ synthetase activities and a decreased hepatic glutathione. However, the extracts obtained from Ullung Is. (native site) were less active than the extracts from Daegwallyoung, suggesting that A. victorialis var. platyphyllum from the cultivated site is more useful for functional food than of native site. These results also suggest that the antihepatotoxic effect is due to a higher content of hepatic glutathione. Gas chromatography of the twelve extracts showed significantly different sulfides, disulfides or trisulfides contents belonging to volatile sulfur substances (VSS). Nine components were identified on the basis of their mass spectra, namely, dimethyl disulfide, dimethyl trisulfide, diallyl disulfide, dipropyl disulfide, allyl methyl sulfide, allyl methyl trisulfide, 2-vinyl-4H-1,3-dithiin, 3,4-dihydro-3-vinyl-1,2-dithiin, and allithiamine. Extract DL-1 had the highest VSS content. Dried plant materials contained larger amounts of the VSSs than other extracts, and the leaves contained larger amount than the bulbs. These results suggest that heat treatment increases the antiheaptotoxic ability of A. victorialis var. platyphyllum by increasing the proportion of VSSs.

Development of a Control Algorithm for a Static VAR Compensator Used in Industrial Networks

  • Spasojevic, Ljubisa;Papic, Igor;Blazic, Bostjan
    • Journal of Power Electronics
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    • v.14 no.4
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    • pp.754-763
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    • 2014
  • In this paper a method for the development of a static VAR compensator (SVC) control algorithm is presented. The proposed algorithm has been designed with the objective of eliminating the negative impact of electric arc furnaces on the power system. First, a mathematical model of the proposed SVC controller in the d-q synchronous rotating coordinate system is developed. An analysis under dynamic and steady state conditions is also carried out. The efficiency of the presented controller is demonstrated by means of computer simulations of an actual steel-factory network model. The major advantages of the proposed controller are better flicker compensation, increased ability to regulate voltage and the need for only one-point network measurements.

Comparison of Fragrance and Chemical Composition of Essential Oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis) (곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 향기성분 비교)

  • Yeon, Bo-Ram;Cho, Hae Me;Yun, Mi Sun;Jhoo, Jin-Woo;Jung, Ji Wook;Park, Yu Hwa;Kim, Songmun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.12
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    • pp.1758-1763
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    • 2012
  • This study was conducted to compare fragrance and volatile chemicals of essential oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis). Essential oils were extracted by steam distillation of leaves of Gom-chewi (GC) and Handaeri Gom-chewi (HGC), after which samples were collected by solid-phase micro extraction and the compositions of the essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The yields of the essential oils in GC and HGC were 0.12% and 0.04%, respectively, and the threshold levels of the essential oils in GC and HGC were 0.01% and 0.1%, respectively. There were 19 constituents of the essential oil of Gom-chewi: 14 carbohydrates, 4 alcohols, and 1 acetate, and the major constituents were L-${\beta}$-pinene (36.02%), D-limonene (25.64%), ${\alpha}$-pinene (24.85%) and ${\beta}$-phellandrene (5.39%). In the essential oil of HGC, 25 constituents were identified: 17 carbohydrates, 4 alcohols, 3 acetates, and 1 N-containing compound, and the major constituents of HGC were D-limonene (39.74%), L-${\beta}$-pinene (35.43%) and ${\alpha}$-pinene (11.94%). The minor constituents of HGC were ${\rho}$-cymene, ${\gamma}$-muurolene, ${\gamma}$-cadinene, germacrene D, ingol 12-acetate and butyl 9,12,15-octadecatriene and nimorazole were not identified in the GC essential oil. Overall, the results showed that the fragrance and chemical compositions of essential oils in GC and HGC differed, suggesting that both essential oils could be used for the development of perfumery products.

The Early Vegetational Succession of he Burned Area in Dangji-Dong (당지동의 산화적지의 초기식생천이)

  • Kim, Woen;Jeong Ho Suh;Chong Un Ri
    • The Korean Journal of Ecology
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    • v.6 no.4
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    • pp.237-242
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    • 1983
  • This is an investigation on the secondary vegetation and succession in the mixed forests destroyed by the severe crown fires on April 8, 1982. The results are summarized as follows: The floristic compositions in the investigated area are composed of 63 kinds of kinds of vascular plants. The biological type shows $ H-D_1, 4-R_5-e$, which is supposed to make a progress to the $ H-D_1-R_5-e$ type. Dominant species are Spodiopogon sibiricus (100.00)-Carex humilis var. nana (70.52)-Miscanthus sinensis var. purpurascens (61, 06)-Lespedeza maximowiczii (57.53). Lespedexa maximowiczii is prevailing as the dominant species in this area. The species diversity(H) and eveness index(e) show 3.00 and 0.74 respectively, so that the species are various and shows uniform distribution comparatively in these communities. Degree of succession(DS) is 479 in the investigated area, the value of which is comparatively higher than that of Chungcheongbuk-do area at the second year afer fires. After the crown and trunk of trees were scorched by the crown fires, the pine tree (Pinus densiflora) died and the other species could be survivd after sometimes. It some that Quercus serrata. Q. dentata, Maackia amurensis, Lespedez cyrtobotrya, Platycarya strobilacea and Lindera glauca are the fire-resistant plants.

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Photochemical Efficiency and psbA Gene Expression of Crinum Leaves under Natural Environmental Stress in Winter (겨울철 저온스트레스에 의한 문주란의 광합성효율과 psbA 유전자의 발현양상)

  • O, Sun Ja;Go, Seok Chan
    • Journal of Environmental Science International
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    • v.13 no.4
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    • pp.359-365
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    • 2004
  • The change of chlorophyll fluorescence parameters, O-J-I-P transients and psbA gene expression were investigated in the leaves of Crinum asiaticum var. japonicum on the natural condition in winter, in order to elucidate physiological responses of photosystem II (PS II) activity to winter stresses. The photochemical efficiencies of PS II, Fv/Fm, were significantly low in winter, contrary to its high value in summer. The values of I -qN and I-qP were lower in midday than at dawn or night both in summer and winter, although their decrease in midday was less in winter than in summer. In the O-J-I-P transients, the fluorescence intensity of J, I, P-step decreased remarkably depending on temperature drop in winter. And the D I reaction center protein of PS II decreased in late winter more than in early winter, concomitantly with relatively high content of description products of psbA gene in midday. These results indicate that low temperature in winter causes irreversible damage to PS II and subsequently leads to cell death.

Georeferencing of Primary Species Occurrence Data and Necessity of Data Quality Control - A Case Study of Two Varieties of Ox-Knee, Achyranthes bidentata Blume - (1차종발생자료를 응용한 지리참조연산표준화 및 자료 품질 관리의 필요성 - 쇠무릎과 털쇠무릎의 적용 사례 -)

  • Chang, Chin-Sung;Chang, Kae Sun;Ahn, Yong-Sup;Kim, Hui
    • Journal of Korean Society of Forest Science
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    • v.101 no.2
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    • pp.185-194
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    • 2012
  • The purpose of this contribution is to develop the framework of a methodology for identifying potential errors in georeferencing and in an application of it using specimens of Ox-Knee, Achyranthes bidentata Blume in Korea. At infraspecific level, uncertainty of identification showed that 41% of A. bidentata var. japonica and 28% of var. bidentata were misidentified, suggesting that the uncertainty level was independent of the reliability of experts' identification. For georeference specimen records, 71 specimens out of total 303 were selected and utilized as occurrence data: Uncertainty was 32.4 km at maximum and was 0.1297 km at minimum (mean = 4,055 m, s.d. = 5,772 m). Var. japonica is common throughout most of the southeastern Korea and west coastal areas, while var. bidentata has been found as far north as Gyeonggi and Gangwon provinces. We modelled the potential distribution of two varieties using Bioclim approach in Korea based on several environmental factors. Our results indicated the most important region for var. japonica lies the west coast ranges and southern area, while for Chungcheongnam-do of potential high diversity occurs for var. bidentata. This study shows that the major factors to determine the distribution patterns of two varieties were thermal factors, rather than precipitation. The Bioclim model using geocode and georeferencing data makes the information increasingly useful and reliable. To improve data quality, it requires full management from data collection to final databases including data cleaning.