Three mild mutant strains of tobacco mosaic virus (TMV) were isolated from Nicotiana tabacum var. Samsun incubated at $38^{\circ}C$ for 10 days after inoculation with a wild type of TMV-OM strain. They were designated into Tg 5272, Tw 227 and Tw 333. All mild strains could be distinguished from TMV-OM by their reactions on different indicator plants. The mild strains induced the mild mottling without distinct symptoms, whereas the wild strain produced severe mosaic, rugose and stunting on tobacco and red pepper plants. Tw 227 and Tw 333 produced smaller necrotic spots than those of Tg 5272 and TMV-OM on N. glutinosa and Datura stramonium. The former two strains also produced ring spots and mosaic on Gomphrena globosa compared with necrotic spots by the latter strains. Three mild strains were serologically identical to TMV-OM. Their physical properties were thermal inactivation point $80-85^{\circ}C$, dilution end point between $10^{-4}\;and\;10^{-6}$, and longevity in vitro 7days or longer. Ultraviolet absorption spectra of purified preparations of the mild strains and TMV-OM were identical, with a minimum at 247nm, a maximum at 260nm, and a slight shoulder at 290nm. Electrophoresis of the strains in polyacrylamide-agarose gel showed that all the strains formed one major band and two minor bands, except for one minor band of Tw 333. However, when sodium dodecyl sulfate was added to the purified viruses before electrophoresis, each strain formed only one major band.
Journal of The Korean Society of Grassland and Forage Science
/
v.20
no.4
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pp.237-242
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2000
This study was conducted to investigate the effects of cutting frequency on dry matter yield and nutritive value between heading and headless varieties of sorghumxsudangrass hybrid in 1998. Treatments were var. TE hay grazer(heading type) and Jumbo(headless type) as main plot, and 1, 2, and 3 cutting times per a year as sub plot. Although plant height of sorghumxsudangrass hybrid was no significant difference among treatments, headless type was very slightly higher than that of heading type, and both of variety decreased with 1, 2, and 3 cutting times in order. Also, dry matter yield of sorghum~sudangrass hybrid was no significant difference between heading and headless type. Dry matter yield of first cutting plot in heading type and in headless type was the higher than those of second and third cutting plot. Acid detergent fiber and neutral detergent fiber of headless type were slightly higher than that of heading type. Acid detergent fiber of sorghumxsudangrass hybrid was highest at first cutting plot in both varieties. Neutral detergent fiber of second cutting plot in heading type and first cutting plot in headless type were the highest respectively. In vitro dry matter digestibility of headless type was very slightly higher than that of heading type. And in vitro dry matter digestibility of second cutting plot was the highest in both varieties. In vitro digestible dry matter of sorghumxsudangrass hybrid was no significant difference among treatments. The results demonstrated that although there was not significant difference, cultivation of headless type and first cutting plot per a year were very good for nutritive value and DM yield of sorghumxsudangrass hybrid as summer forage crops in Korea. (Key words : Heading, Headless type, Cutting frequency, Sorghum X Sudangrass Hybrid)
Presently, there is increasing demand for geriatric nursing care due to increasing proportion of elderly population and extended age. And, of particular importance in deforming the effectiveness of nursing care given to the elderly is the attitude of nursing personnel toward the elderly patient. This study was intendent to investigate the attitudes of baccalaureate nursing students toward the elderly, and to investigate any possible correlation between these attitudes and the following variables: the students' levels (freshman, sophomore, junior, senior), their preferences for var-ious fields of specialization in nursing after graduation, their clinical experiences with geriatric-patients, their personal experience with elderly family members, their educational experience of the elderly's health problems and geriatric nursing, and their experience with relatives and acquaintances over 60 years of age. Therefore, the purpose of this study was to compile basic data about the attitudes of nursing students toward the elderly in order to contribute to the improvement of geriatric nursing. The subjects for this study were 259 nursing students: 163 in the College of Nursing at E-womans University., and 96 in the Department of Nursing in K. College. Data was collected from the 11th of Sep., to the 8th of Oct., 1981, using the Tuckman-Lorge Attitude Questionnaire which contained 131 items excepting 6 items. The attitudes toward the elderly was scored by the use of mean and standard deviation. The relations between attitudes toward the elderly and six independent vairables were analysed by T-test. The results which were obtained are as follows : 1. The attitude score of nursing students was M=71.67 S.D= 16.18. Nursing students agreed with 54.8% of the stereotypic statements, and they had stereotypic attitudes toward the elderly relatively. (P<0.01). 2. There was a significant difference between the attitudes of nursing students toward the elderly, and sophomores had a less stereotypic attitudes than juniors and seniors (P<0.01). 3. There was significant difference between the attitudes toward the elderly and the preferences for various fields of specialization in nursing after graduation, and nursing students those who wanted maternity fields had a more stereotypic attitudes than others (P<0.05, p<0.01). 4. There was no significant difference between the attitudes toward the elderly and the clinical experience with the geriatric patients (P>0.05). 5. There was no significant difference between the attitude3 toward the elderly and the personal experience with elderly family members (F>0.05). 6. There was no significant difference between the attitudes toward the elderly and the educational experience of the elderly's health problems and geriatric nursing (P>0.05). 7. There was no significant difference between the attitudes toward the elderly and the experience with relatives and acquaintances over 60 years of age (P>0.05).
Journal of the Korean Society of Environmental Restoration Technology
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v.10
no.2
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pp.84-96
/
2007
The purpose of this study was to select herbaceous plants that can be used for presenting various views and biodiversity on the extensive rooftop greening. Experiment plots were constructed in July 2005 on the rooftop of the Administration Building in the Seoul Women's University. For this experiment, planters were used to design rooftop greening. The size of a planter is 500mm${\times}$500mm${\times}$100mm and each planter has the water storage plate in the lower part of it. The soil was constructed by mixing pearlite, vermiculite, cocopeat, and leaf mold in the ratio of 6 : 2 : 1 : 1. The plot was divided into the watered plot and the dry plot. Since each plot was constructed 2 times, finally 4 planters were constructed in total. One hundred species were used for the experiment and 9 plants per species were planted in each planter. Plants were organized according to types of plants and the experiment used 86 native herbaceous plants, 6 herbs, and 8 foreign plants. The plots were monitored once a month, from July to November 2005. The length and width of plants were tape-measured and covering rate was calculated by CAD program. "SPSS 10.1" was used for a statistical analysis. The result showed no significant difference between the watered plots and the dry plots. In cases of some plants, there were statistically significant differences between the watered planter and dry planter such as follows : Astilbe chinensis and Polygonatum odoratum which are shade plants were measured as the highest value on the watered pots, and Aquilegia buergeriana, Chrysanthemum zawadskii, Calendula arvensis and Gypsophila cerastioides D.Don which are sunny plants were measured as the highest value on the dry plots. According to the final analysis of the data collected and observed for growth condition during the first year of the research, 51 species including Prunella vulgaris var. lilacina and Veronica linaiaefolia in native herbaceous plant, 5 species with Lavandula angustifolia in herbs, and 3 species with Lantana camara and Muscari armeniacum in foreign plants showed the highest growth condition. In conclusion, it is suggested that various plants including sedums could be effectively used for extensive rooftop greening to improve landscape(a view) of the rooftop and increase ecological values.
We investigated the flora of Ibamsan Mt. and Baekamsan Mt., and discuss here important vascular plants found on these mountains. We created a plant list using voucher specimens collected from Ibamsan Mt. and Baekamsan deposited at CNU. There are 769 taxa in Ibamsan Mt. and Baekamsan Mt., consisting of 116 families, 403 genera, 687 species, 16 subspecies, 58 varieties, and 8 forms. Twenty-four endemic plants of Korea were found, including Coreanomecon hylomeconoides Nakai and Eranthis byunsanensis B. Y. Sun. There are 15 rare plants on Ibamsan Mt. and Baekamsan as designated by the Korea Forest Service, including Iris koreana Nakai and Chamaegastrodia shikokiana Makino & F. Maek., as well as 14 red-list plants as designated by the Ministry of the Environment, including Lycoris chinensis var. sinuolata K. Tae & S. C. Ko ex K. Tae & S. C. Ko and Cymbidium macrorhizon Lindl. In total, there are 133 taxa of floristic regional indicator plants, including four level V taxa, such as Iris koreana Nakai; ten level IV taxa, such as Lindera sericea (Siebold & Zucc.) Blume; 28 level III taxa, such as Botrychium japonicum (Prantl) Underw.; 22 level II taxa, including Hylomecon vernalis Maxim.; and 69 level I taxa, including Dryopteris erythrosora (D. C. Eaton) Kuntze. There are 37 invasive plants, including Rumex acetosella L. and Symphyotrichum pilosum (Willd.) G. L. Nesom.
Kim, Seung-Tae;Kim, Won-Hee;Kim, Young-Jin;Huh, Kun-Yang;Lee, Eun-Kyung;Park, Pil-Man
Korean Journal of Breeding Science
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v.41
no.4
/
pp.552-555
/
2009
A new standard rose cultivar "Snow Day" was bred from the cross between pink standard cultivar "Saphia" and pink standard cultivar "Bridal Beauty" at the National Institute of Horticulture & Herbal Science. The cross was made in 2003 and "Snow Day" was finally selected in 2008 after evaluating characteristics for three years from 2006 to 2008. A white standard cultivar, "Snow Day" grows vigorously and resistant to powder mildew. The major characteristics of this cultivar are $152stems/m^2/year$ in yield, 81.8 cm in length of cut flower, 11.3 cm in flower diameter, 29.2 in petal number, and 13.7 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference for this cultivar is relatively higher than that for the control cultivar, "Tineke".
Incidence of the Fusarium wilt caused by F. oxysporum f. sp. vasinfectum of sesame (var. Kwangsan) was remarkably influenced by seeding date and mean air temperature in the field of two or three year's continuous cropping with sesame in 1983 and 1985. Sesame were seeded on six different dates from April 20 to July 5. Air temperature was checked daily at the meteorological station near the experimental field. Low($16{\sim}20^{\circ}C$) and high temperature($20{\sim}25^{\circ}C$) periods were provisionally devided, based on every ten-day mean daily temperature during field experiment for last ten years, which corresponded to before and after June 15 in Jinju, Gyeong-nam. Infection rates were 83.7%, 68.2% and 59.4% in the plants grown for 55 days (seeding date: April 20), 40 days(May 5) and 25 days(May 20) under low temperature. On the other hand, infection rates were below 3% in those plots seeded during high temperature period. The longer the growth period exposed to low temperature, the higher was infection rates. It is interesting to note that 40 days old seedling or older are prone to severe infection compared to the younger ones, in higher temperature of $20{\sim}25^{\circ}C$. Therefore, seedlings in vegetative growth stage are less prone to infection than these in reproductive growth stage. The result showed that air temperature during sesame growth was one of the most important factor affecting the incidence of Fusarium wilt. This suggested that sesame crop, which is of tropical origin, has been predisposed to Fusarium wilt, when the plants were exposed to low temperature of $16{\sim}20^{\circ}C$.
The present study was undertaken to classify and describe the spring naturalized plant communities in old-Andong city by the methodology of the ZM school of phytosociology. As a result, the vegetation was classified into the six communities and two subcommunities: A. Bromus tectorum community A-a. Bromus japonicus subcommunity, A-b. Melica onoei subcommunity; B. Poa pratensis community; C. Rumex crispus community; D. Oenothera lamarckiana community; E. Ambrosia artemisiifolia var. eiatior community; F. Rumex conglomeratus community. The total taxa of the naturalized plant communities consisted of 31 families, 86 genera, 114 species and 13 varieties. Of which the taxa of the naturalized plant species consisted of 8 families, 21 genera, 25 species and 1 variety. The result of Bray-Curtis ordination revealed that the plots suveyed were arranged according largely to the vegetation units of' the communities. Also the interspecific affinity was examined by an analysis of interspecific association and the main component species in the communities were divided into two groups. The naturalized rate was higher in the communitise affected by strong human impacts, while was lower in the communities affected relatively less by human impacts. On the other hand the seasonal changes of the communities and the naturalized rate between the spring and the autumn were investigated. Many naturalized communities present in Spring were replaced by the other native ruderal communitues in the Autumn. The naturalized rate based on the dominance was largely decreased over from the spring to the Autumn.
This study conducted health assessment and multivariate vegetation analysis using the riparian vegetation index in 30 sites of the Geumgang mainstream and Mihocheon to obtain practical data on the river management of the Geumgang. The result showed that the number of plant communities was 54. The flora was 75 families, 185 genera, 243 species, 2 subspecies, 21 varieties, 2 varieties, and 268 taxa. The riparian vegetation index was 38.3 (3.3; G-D1 ~ 66.7; G-U2, G-U4, and G-M3), and the health of the rivers in this area was evaluated as normal (grade C). The health of rivers was the highest in the upper stream of Geumgang mainstream and lowest in the downstream of Geumgang mainstream. The relationship between riparian vegetation index and chlorophyll-a content was low. The riparian vegetation was divided into five groups of Digitaria ciliaris colony group, Salix gracilistyla colony group, Erigeron annuus colony group, the group dominated by Humulus japonicus, Salix koreensis, Miscanthus sacchariflorus, and Phragmites japonica colonies, and the group dominated by Conyza canadensis and Echinochloa crusgalli var. echinata colonies. They had the similar health conditions. The CCA analysis showed that the environmental factors affecting the distribution of vegetation were physical factors such as vegetation area, artificial structure area, waterway area, branch width, channel width, and bank height and the biological factors such as the number of species. As such, it is necessary to maintain the health condition through continuous monitoring where the health condition is high and to apply active measures such as ecological restoration where the health condition is low.
To discover new functional materials using edible plants, antioxidant activities of methanol extracts from various parts of seven wild vegetables were investigated in vitro. Total polyphenol contents, determined by Folin-Denis method, varied from 16.74 to $130.22{\mu}g/mg$. Radical-scavenging activities of methanol extracts were examined using ${\alpha},\;{\alpha}-diphenyl-{\beta}-pirrylhydrazyl$ (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay. Inhibition effects on peroxidation of linoleic acid determined by ferric thiocyanate (FTC) method and on oxidative degradation of 2-deoxy-D-ribose in Fenton-type reaction system were dose-dependent. Athyrium acutipinulum Kodama (leaf and rood), Achyranthes japonica (Miq.) Nakai (seed), and Solidago virga-aurea var. gigantea Nakai (root) showed relatively high antioxidant activities in various systems.
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