• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.33-45
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• 2011

Heat shock protein 90 (Hsp90) is ATPase-directed molecular chaperon and affects survival of cancer cell. Inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the cancer cell was reported. However, its role during oocyte maturation and early embryo development is very insufficient. In this study, we traced the effects of Hsp90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on meiotic maturation and early embryonic development in pigs. We also investigated several indicators of developmental potential, including structural integrity, gene expression (Hsp90-, cell cycle-, and apoptosis-related genes), and apoptosis, which are affected by 17-AAG. Then, we examined the roles of Hsp90 inhibitor on viability of primary cells in pigs. Porcine oocytes were cultured in the NCSU-23 medium with or without 17-AAG for 44 h. The proportion of GV arrested oocytes was significantly different between the 17-AAG treated and untreated group (78.2 vs 34.8%, p<0.05). After completion of meiotic maturation, the proportion of MII oocytes was lower in the 17-AAG treated group than in the control group (27.9 vs 71.0%, p<0.05). After IVF, the percentage of penetrated oocytes was significantly lower in the 17-AAG treated group (25.2%), resulting in lower normal pronucleus formation (2PN of 14.6%). Therefore, the inhibition of meiotic progression by Hsp90 inhibitor played a critical role in fertilization status. Porcine embryo were cultured in the PZM-3 medium with or without 17-AAG for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without 17-AAG. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that the number of containing fragmented DNA at the blastocyst stage increased in the 17-AAG treated group compared with control (7.5 vs 4.4, respectively). Blastocysts that developed in the 17-AAG treated group had low structural integrity and high apoptotic nuclei than those of the untreated control, resulting in decrease the embryonic qualities of preimplantation porcine blastocysts. The mRNA expressions of cell cycle-related genes were down-regulated in the 17-AAG treated group compared with control. Also, the expression of the pro-apoptotic gene Bax increased in 17-AAG treated group, whereas expression of the anti-apoptotic gene Bel-XL decreased. However, the expression of ER stress-related genes did not changed by 17-AAG. Cultured pESF cells were treated with or without 17-AAG and used for MIT assay. The results showed that viability of pESF cells were decreased by treatment of 17-AAG ($2{\mu}M$) for 24 hr. These results indicated that 17-AAG decreased cell proliferation and increased cell death. Expression patterns Hsp90 complex genes (Hsp70 and p23), cell cycle-related genes (cdc2 and cdc25c) and apoptosis-related genes (Bax and Bcl-XL) were significantly changed by using RT-PCR analysis. The spliced form of pXbp-1 product (pXbp-1s) was detected in the tunicamycin (TM) treated cells, but it is not detected in 17-AAG treated cells. In conclusion, Hsp90 appears to play a direct role in porcine early embryo developmental competence including structural integrity of blastocysts. Also, these results indicate that Hsp90 is closely associated with cell cycle- and apoptosis-related genes expression in developing porcine embryos.

• Journal of Life Science
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• v.9 no.4
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• pp.395-407
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• 1999

The queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1997 and reared under the various temperatures as 15$^{\circ}C$$\pm$1$^{\circ}C$, 2$0^{\circ}C$$\pm$1$^{\circ}C$, $25^{\circ}C$ $\pm$1$^{\circ}C$, 3$0^{\circ}C$$\pm$1$^{\circ}C$, 60%$\pm$5% of relative humidity and 14L : 10D, to investigate their ecological characteristics, as comparing with control(29$^{\circ}C$$\pm$1$^{\circ}C$, 60%$\pm$5% of relative humidity and red light). As a result, eggs were laid by foundation queen at 15$^{\circ}C$, but they didn't hatched out while at 2$0^{\circ}C$ eggs were laid by them and they hatched out. When a foundation queen laid eggs at $25^{\circ}C$ and they all were hatched out and normally grown. Finally they were developed to the normal colony. B. ignitus worker survived for 77days at 2$0^{\circ}C$, about 69days at $25^{\circ}C$, about 68days at 3$0^{\circ}C$ and about 63days at 29$^{\circ}C$(control). The first brood workers emerged in 25-27days from the egg which a foundation queen laid. At the various temperatures, the captive queens stand to lay eggs in about 18days at 15$^{\circ}C$, 15days at 2$0^{\circ}C$, 11days at $25^{\circ}C$, 11days at 3$0^{\circ}C$ and 4days in control. The first worker and the first drone from the egg cell of the collected queens appeared in about 27days and 72days at 2$0^{\circ}C$, 26days and 88days in $25^{\circ}C$, 24days and 65days at 3$0^{\circ}C$, 25days and 71days in control, respectively. In colony foundation, 33% of the collected queen at 2$0^{\circ}C$ and $25^{\circ}C$, 100% of the disposed queen at 3$0^{\circ}C$ and in control, and 67% of them at 3$0^{\circ}C$ and in control both produced new queens in 66days and 88days, respectively. The life span of the colony founded covered fro about 3 months at $25^{\circ}C$, 3$0^{\circ}C$ and in control. At lower temperatures, the life span of queen is shorter; 2 months at 15$^{\circ}C$ and 3 months at 2$0^{\circ}C$, respectively. A colony which normally developed, varied in size with rearing temperatures; about 20heads at 2$0^{\circ}C$, 482heads at $25^{\circ}C$, 330heads at 3$0^{\circ}C$ and 452heads in control. A foundation queen monthly oviposited 1.5egg cells at 15$^{\circ}C$, 3.0egg cells at 2 $0^{\circ}C$, 21.7egg cells at $25^{\circ}C$, 42.3egg cells at 3$0^{\circ}C$ and 47.0egg cells in control. As a colony developed in June and July, egg cells as well as daily average cells increased in number, as compared to those in May and August. Also, in June and July, interval(days) of egg laying is shorter than in May and August. Number of nectar pots during the colony development varied with rearing temperatures; 31pots at $25^{\circ}C$, 39pots at 3$0^{\circ}C$, 23pots in control. The emergence of new queen showed a distinctively different two patterns; early emerging type and late emerging type. Workers are rapidly increased from early in July to early in August at $25^{\circ}C$, but at 3$0^{\circ}C$ and in control, emergence of workers are remarkably increased from the middle of June and last until July. No new queen emerged at 15$^{\circ}C$, 2$0^{\circ}C$ and $25^{\circ}C$. New queens at 3$0^{\circ}C$ and in control emerged between late in June and early in July. Thus emergence of new queen was temperature dependent.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.649-658
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• 2003

Early introducing the ducks into the paddy field involves a variety of environmental changes such as new surroundings and contact with water, so that some free-ranged ducks show behavioral and physiological changes indicative of stress or die from not adapted for new circumstances. Moreover, the free-ranged ducks was tread on the rice plant, and required a great deal of labor and time for captured the ducks after finishing the introducing. The aim of the present study was to examine the effect of accustoming and imprinting of duckling on behavior patterns, body weight gain and yield of the rice plant in paddy fields. Three paddy plots were used as control (no imprinting and no contact with people), taming (imprinting and regular handling) and roughness (handling roughly and strike terror to ducks) plots. Right after introducing the ducks into the paddy field, eating and moving behaviour of taming plot ducks tended to be longer time spend than that of other treatments. However, eating and moving time tended to be longer in the control than that of other two treatments on the 2 weeks after. Flighting distance was not showed in the free-ranging period taming plot, but the control plot was significantly (P<0.05) longer than the roughness plot. The captured time of free-ranged ducks tended to be shorter in the order of taming, control and roughness plots. Body weight gains was not significant. The number of rice plants damaged by free-ranging ducks in the taming plot were significantly (P<0.05) less than that in the control plot, but the yield and yield components of the rice plant were not differ among 3 treatments. These results indicated that the imprinting or regular handling and related treatments of duckling was reducing badly damage of rice plants, captured time and labor of free-ranged ducks in paddy field, although the working behavior of ducks and yield ability of the rice plants were not affected.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.289-296
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• 2002

A broiler experiment was conducted to investigate the effect of supplementing yeast culture (Saccharomyces cerevisiae, Pichia pastoris) on the growth performance, small intestinal microflora and immune response in broiler chickens. One thousand hatched broiler chickens(Ross$^{(R)}$) were assigned to 6 treatments: control (basal diet), CTC; chlorotetracycline 100ppm, YC-SC; yeast culture(Saccharomyces cerevisiae) 0.3%, YC-PP; yeast culture(Pichia pastoris) 0.3%, RPPC-0.1; refined Pichia pastoris culture 0.1%, RPPC-0.3; refined Pichia pastoris culture 0.3%. There were no significant differences in growth, feed intake, feed efficiency and mortality among the treatments. However, chickens fed diets with yeast cultures showed numerically higher weight gain than those fed the control diets. Supplementation of yeast cultures and CTC improved feed efficiency and decreased mortality compared to control. Nutrient digestibilities were not affected by the dietary treatments. Total number of Lactobacilli in small intestine was higher while that of Cl. perfringens was lower with yeast culture treatments than control. Small intestine E. coli population of RPPC-0.3 treatment was significantly lower than that of the control. The serum IgG concentration tended to be higher in broilers fed yeast cultures than those fed the control and CTC diet. In conclusion, the supplementation of yeast culture products showed, although not significant but, numerical advantages in productivity and profile of microbial flora and serum IgG compared to the control and CTC supplementation.

• Journal of Applied Biological Chemistry
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• v.54 no.4
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• pp.279-283
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• 2011

Toll-like receptors (TLRs) play an important role in induction of innate immune responses. The activation of TLRs triggers inflammatory responses that are essential for host defense against invading pathogens. Phenethyl isothiocyanate (PEITC) extracted from cruciferous vegetables has an effect on anti-inflammatory therapy. Dysregulated activation of nuclear factor-${\kappa}$B (NF-${\kappa}$B), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) has been shown to play important roles in the development of certain inflammatory disease. To evaluate the therapeutic potential of PEITC, NF-${\kappa}$B activation and COX-2 and iNOS expression induced by lipopolysaccharide (LPS, TLR4 agonist), polyinosinic-polycytidylic acid (Poly[I:C], TLR3 agonist), 2 kDa macrophageactivating lipopeptide (MALP-2, TLR2 and TLR6 agonist) or oligodeoxynucleotide 1668 (ODN1668, TLR9 agonist) were examined. PEITC inhibits the activation of NF-${\kappa}$B induced by LPS or Poly[I:C] but not by MALP-2 or ODN1668. PEITC also suppressed the iNOS expression induced by LPS or Poly[I:C]. However, PEITC did not suppress COX-2 expression induced by LPS, Poly[I:C], MALP-2, or ODN1668. These results suggest that PEITC has the specific mechanism for antiinflammatory responses.

• The Korean Journal of Pharmacology
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• v.18 no.2
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• pp.15-25
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• 1982

It was undertake to investigate the factors involved in the micro thrombus formation in the plasma from the patients with cerebrovascular disease(CVD) and the in vitro actions of sodium nitroprusside on the platelet aggregate formation. 1) The microthrombus formation in the plasma from CVD was significantly enhanced, in comparison with that from the healthy volunteers. 2) Both lipid peroxide and cathepsin D in the plasma from CVD were higher than those levels from the healthy volunteers. 3) Whereas the platelets from healthy individuals showed less aggregation activity in response to ADP in the second phase those from CVD revealed the enhanced aggregating response to ADP. 4) When the bovine basilar artery, rabbit aorta and human umbilical artery were pretreated with $K^+-free$ PSS, ouabain, 13-hydroperoxylinoleic acid(13-HPLA) and cadmium they markedly enhanced the platelet aggregability respectively. 5) Platelet aggregation induced by $K^+-free$ PSS-treated bovine basilar artery was decreased by sodium nitroprusside in a dose-dependent manner, but not by either hydralazine. 6) Both dibutyryl cyclic AMP and 8-bromo cyclic GMP had the inhibitory action on the platelet aggregation. However, the latter had more prominent action than former. The antiaggregating effect by sodium nitroprusside was antagonized by pretreatment with methylene blue, but not by hemoglobin. These results provide the evidences for the therapeutic use of sodium nitroprusside in the emergency of cerebrovascular disease and in remains the further study of the clinical therapy with it.

• The Korean Journal of Pesticide Science
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• v.17 no.2
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• pp.126-132
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• 2013

The objectives of this study were to provide the basic data of the residue of deltamethrin in the stream water. Deltamethrin was treated on side of Ban-Suk stream and Juk-Dong ditch for hygienic purpose by air spray. The drift concentration of deltamethrin was investigated with different wind speed condition on Ban-Suk stream (A), and the change of residue with time course on Juck-dong ford (B). Also we found the residual change of deltamethrin until 48 hour in Yu-Seong stream confluence (C) where two streams join. Maximum residues of A were $0.17{\mu}g/L$ (5 min, 200 m) at strong wind speed and $4.42{\mu}g/L$ (0 min, 25 m) at moderate wind speed according to different wind velocity. Residues of B were $0.15{\sim}0.26{\mu}g/L$ (0~480 min) after spraying, and decreased to a non-detected level after 720 min. Residues of C were $0.15{\mu}g/L$ (0 min), $0.11{\mu}g/L$ (1 min) and $0.10{\mu}g/L$ (12 hr) after spraying, and no residues were detected in any other samples. From these results, it is concluded that deltamethrin residues in water should be rapidly diluted into stream water and affected negligible toxic effect to stream ecosystem.

• Korean Journal of Weed Science
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• v.16 no.2
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• pp.81-87
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• 1996

Seeds of rice, cv. Ilpoom, and barnyardgrasses(Echinochloa crus-galli, vars. oryzicola, crux-galli, and praticola) were sown for a characterization of their responses to temperature during emergence under a dry direct-seeded condition. A laboratory-made aluminum block apparatus for emergence-temperature control conferred a linear continuous temperature gradient from 10 to $30^{\circ}C$ to the seeds from cooling to heating ends of the apparatus. The lowest temperature for emergence was $12.3^{\circ}C$ for rice cv. Ilpoom, and $11.0^{\circ}C$ for the three varieties of Echinochloa spp.. Percent emergence of rice increased sharply with an increase in temperature by ca. $20^{\circ}C$, then leveled-off, while those of barnyardgrasses increased almost linearly with temperatures up to $30^{\circ}C$. In rice the time required for emergence after seeding was shortened exponentially with increased temperature while those for barnyardgrasses were shortened almost linearly from 11 to $30^{\circ}C$. The temperature-response characteristic of rice in emergence-speed was almost the same among those for the 1st emergence, emergence by 25, 50, 75%, or average emergence time. At $13^{\circ}C$, $346.7^{\circ}C$ days of accummulated temperature(26.67 days) were required for the 1st emergence in rice while 131.7, 136.0, and $138.7^{\circ}C$ days(10.13, 10.46, and 10.67 days) were required for the 1st emergence in E. spp., vars. crus-galli, praticola, and oryzicola, respectively. Greater cold tolerance and increasingly faster emergence of barnyardgrasses than rice below $20^{\circ}C$ seem to render the barnyardgrasses as much more competitive than rice at lower temperatures.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.9 no.2
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• pp.201-219
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• 2014

The Republic of Korea in the real economy to a knowledge economy, and a center of creativity and imagination in the creative economy is changing the paradigm. As the core of creating economic, creative industries with the technology and information play an important role in the industry individuals. In order to solve the problem of the polarization of the economy and high youth unemployment rate of Korea, to recognize the role of the creative industries, as objection part, dimensions pan-national and one creative companies in industries of Mobile Apps various policies that support has been promoted. Support these policies to be able to contribute to the establishment of the success of mobile apps one-person creating company, we performed this study targeting one-person company that creates mobile apps area, we conducted a demonstration study of success factors, and thus more effective and efficient in an attempt to seek out support measures. In this study, we derive a research 4 hypothesis about the success factors of one creative enterprise through literature discussion, a study was made on the basis of empirical data of one-person company that creates mobile apps. The results of the analysis, first, if the development rate of the mobile application technology is fast and a new competition associated product is appeared, it was possible to find a tendency to be higher at the performance quantitative companies. Second, if the founder is a founding for the benefit and rewarding work and come to terms with the risk, it was possible to discover tends to be higher achievement quantitative. Third, if one-person company select a target market with capture intensively, it was possible to find a tendency for higher qualitative results. Fourth, it could be found that the reliability of the contact frequency of the network related performance business environment these characteristics enterprise management strategy and act as a significant modulatory effect. Provision of information relating to management and entrepreneurship education to be one creative enterprise is required, these results suggest that there is a provision continuing need for the opportunity to be able to meet and network and reliable variety have. In this study, to take advantage to promote the elimination measures that can increase the likelihood of success of the company of institutions to support one company that creates knowledge-based, such as in the field of mobile application.

• Journal of Energy Engineering
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• v.23 no.4
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• pp.61-72
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• 2014

This paper analyzed transition pathways toward a low carbon society in Korea to meet global $2^{\circ}C$ climate target. Lower economic growth, industrial structure change, enhance of energy demand management, decarbonization of power sector, and replacement of low carbon fuel could reduce greenhouse gas (GHG) emission from fuel combustion in 2050 by 67% against in 2011, or by 74% against in BAU (Business-As-Usual). Lower economic growth contributes to 13% of cumulative emission reduction relative to BAU, industrial structure change 9%, enhance of energy demand management 72%, decarbonization of power sector 5% and replacement of low carbon fuel 1% respectively. Final energy consumption in 2050 needs to be reduced to 50% relative to 2011, or to 41% relative to BAU. Nuclear, coal and renewable energy represent 31%, 40%, 2% respectively among electricity generation in 2011, but 38%, 2%, 32% in 2050. CCS represents 23% of total generation in 2050. Emission intensity of electricity in 2050 was decreased to 19% relative to 2011, or to 24% relative to BAU. Primary energy in 2050 was decreased to 64% compared to 2011, or to 44% compared to BAU. Final energy consumption, primary energy supply and GHG emission from fuel combustion from 1990 to 2011 increased by 176%, 197%, 146%. Radical change from historical trend is required to transit toward a low carbon society by 2050. Appropriate economic growth, structural change to non-energy intensive industries, energy technology research, development and deployment (RD&D) in terms of enhancement of energy efficiency and low carbon energy supply technologies, and fuel change to electricity and renewable energy are key instruments.