• Physical Therapy Rehabilitation Science
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• 제2권1호
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• pp.12-20
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• 2013

Objective: To determine the energy consumed and muscle use during dance compared to different standard exercise devices. Design: Longitudinal study. Methods: Fifteen female subjects were evaluated to assess the energy cost and muscle activity during a 20 minute dance video compared to treadmill, elliptical track and bicycle ergometry. The later 3 forms of exercise were accomplished in four, 5 minute bouts at different intensities of exercise. Subjects were in the age range of 22-24 years old, were free of cardiovascular disease and did not have any neurological injuries. They were not sedentary and exercised at least twice a week. During the exercise, muscle activity was measured by the electromyogram recorded by surface electrodes on 6 muscle groups. A Cosmed metabolic cart was used to measure oxygen consumption during the exercise. Results: The aerobic dance video that was tested here was equivalent to a hard workout on any of the 3 exercise modalities. The dance routine was equivalent in terms of energy consumed to running at 225 watts of work or running for 20 minutes at a speed of 2 meters per second (4.47 miles per hour). Compared to the bicycle, it was equivalent to cycling at 112 watts for 20 minutes (2.25 kpm), and for the elliptical trainer, dance was equivalent to 435 watts. Concerning muscle use, the dance routine was the most balanced for upper, core and lower body muscles. Although the elliptical trainer was close, it required muscle less muscle use. Conclusion: A good dance video can be more effective than standard exercise equipment.

• Asian Pacific Journal of Cancer Prevention
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• 제15권12호
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• pp.5001-5007
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• 2014

The acetyltransferase inhibitor garcinol, a polyisoprenylated benzophenone, is extracted from the rind of the fruit of Garcinia indica, a plant found extensively in tropical regions. Anti-cancer activity has been suggested but there is no report on its action via inhibiting acetylation against cell proliferation, cell cycle progression, and apoptosis-inhibtion induced by estradiol ($E_2$) in human breast cancer MCF-7 cells. The main purposes of this study were to investigate the effects of the acetyltransferase inhibitor garcinol on cell proliferation, cell cycle progression and apoptosis inhibition in human breast cancer MCF-7 cells treated with estrogen, and to explore the significance of changes in acetylation levels in this process. We used a variety of techniques such as CCK-8 analysis of cell proliferation, FCM analysis of cell cycling and apoptosis, immunofluorescence analysis of NF-${\kappa}B$/p65 localization, and RT-PCR and Western blotting analysis of ac-H3, ac-H4, ac-p65, cyclin D1, Bcl-2 and Bcl-xl. We found that on treatment with garcinol in MCF-7 cells, $E_2$-induced proliferation was inhibited, cell cycle progression was arrested at G0/G1 phase, and the cell apoptosis rate was increased. Expression of ac-H3, ac-H4 and NF-${\kappa}B$/ac-p65 proteins in $E_2$-treated MCF-7 cells was increased, this being inhibited by garcinol but not ac-H4.The nuclear translocation of NF-${\kappa}B$/p65 in $E_2$-treated MCF-7 cells was also inhibited, along with cyclin D1, Bcl-2 and Bcl-xl in mRNA and protein expression levels. These results suggest that the effect of $E_2$ on promoting proliferation and inhibiting apoptosis is linked to hyperacetylation levels of histones and nonhistone NF-${\kappa}B$/p65 in MCF-7 cells. The acetyltransferase inhibitor garcinol plays an inhibitive role in MCF-7 cell proliferation promoted by $E_2$. Mechanisms are probably associated with decreasing ac-p65 protein expression level in the NF-${\kappa}B$ pathway, thus down-regulating the expression of cyclin D1, Bcl-2 and Bcl-xl.

• Wind and Structures
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• 제12권4호
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• pp.383-400
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• 2009

Low rise building roofs can be subjected to large fluctuating pressures during a tropical cyclone resulting in fatigue failure of cladding. Following the damage to housing in Tropical Cyclone Tracy in Darwin, Australia, the Darwin Area Building Manual (DABM) cyclic loading test criteria, that loaded the cladding for 10000 cycles oscillating from zero to a permissible stress design pressure, and the Experimental Building Station TR440 test of 10200 load cycles which increased in steps to the permissible stress design pressure, were developed for assessing building elements susceptible to low cycle fatigue failure. Recently the 'Low-High-Low' (L-H-L) cyclic test for metal roofing was introduced into the Building Code of Australia (2007). Following advances in wind tunnel data acquisition and full-scale wind loading simulators, this paper presents a comparison of wind-induced cladding damage, from a "design" cyclone proposed by Jancauskas, et al. (1994), with current test criteria developed by Mahendran (1995). Wind tunnel data were used to generate the external and net pressure time histories on the roof of a low-rise building during the passage of the "design" cyclone. The peak pressures generated at the windward roof corner for a tributary area representative of a cladding fastener are underestimated by the Australian/New Zealand Wind Actions Standard. The "design" cyclone, with increasing and decreasing wind speeds combined with changes in wind direction, generated increasing then decreasing pressures in a manner similar to that specified in the L-H-L test. However, the L-H-L test underestimated the magnitude and number of large load cycles, but overestimated the number of cycles in the mid ranges. Cladding elements subjected to the L-H-L test showed greater fatigue damage than when experiencing a five hour "design" cyclone containing higher peak pressures. It is evident that the increased fatigue damage was due to the L-H-L test having a large number of load cycles cycling from zero load (R=0) in contrast to that produced during the cyclone.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.104-109
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• 2003

Enhancement of the production of benzoylformate reductase (BFR) was attempted under oxidative stress established by natural electron carriers. -lipoic acid (LA), flavin adenine dinucleotide (FAD), and ubiquinone (UQ) did not inhibit growth of E. faecalis when their concentrations were as high as $10{\mu}M$, while $H_2O_2$ and methyl viologen ($MV^2+$) inhibited the bacterial growth. BFR activity in the bacterial extract had increased rapidly after 1 h of cultivation after the addition of $4{\mu}M$ of natural electron carriers, and the activity was maintained during further cultivation. BFR activity of the cells treated with the natural electron carriers was $40\%$ higher than that of the control. In the presence of $4{\mu}M\;H_2O_2\;and\;MV^2+$, BFR activity increased, reaching the highest activity at about 5 h cultivation, and then decreased with further cultivation. It seems that natural electron carriers not only stimulate the induction of BFR, but also stabilize the enzyme. BFR was hardly affected by LA, FAD, and UQ, while $H_2O_2\;and\;MV^2+$ inactivated the crude enzyme. The decrease of BFR activity in the presence of $H_2O_2\;and\;MV^2+$ might be ascribed to inactivation of the enzyme by the oxidants.

• Elastomers and Composites
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• 제47권4호
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• pp.355-363
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• 2012

2,2-Bis(3-amino-4-hydroxyphenyl)hexafluoropropane 와 방향족 디이미드-디카르복실산을 thionyl chloride, triethyl amine 및 NMP 하에서 직접 중축합하여 trifluoromethyl group을 포함하는 일련의 poly(hydroxyamide)s(PHAs)를 제조 하였다. PHAs은 $35^{\circ}C$ DMAc 용액에서 0.54-0.96 dL/g의 고유점도를 보였다. 모든 PHAs은 여러 종류의 유기용매에 쉽게 용해되었으나, PBOs은 황산에 부분적으로 용해되는 것을 제외하고, 전혀 용해되지 않았다. PHAs은 열적고리화 반응에 의해서 흡열과 함께 PBOs로 전환되었다. PHAs의 최대 중량손실온도는 $559-567^{\circ}C$의 영역을 보였고, poly(benzoxazole imide)s(PBOs)은 47-59%의 비교적 높은 char 수득율을 보였다. PBOs의 Pyrolysis Combustion Flow Calorimeter(PCFC) 결과에서 12-19 W/g의 HRR 과 2.7-3.6 kJ/g의 total HR을 보였다. PBO 1의 HRR은 PBO 3 의 HRR (19 W/g)보다 37 % 낮은 12 W/g의 가장 낮은 값을 보였다.

• 생태와환경
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• 제50권4호
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• pp.441-451
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• 2017

2016년 8월 19일부터 12월 31일까지 현장원수와 퇴적물이 주입된 현장 배양장치 실험을 통해 환경요인의 영향에 따른 식물플랑크톤 기능그룹(phytoplankton functional group, PFG)의 천이 특성을 연구하였다. 본 연구에서 확인된 식물플랑크톤은 총 27속 50종이었으며, 분류군별로는 녹조류 30종, 규조류 11종, 남조류 7종, 편모조류 2종으로 나타났다. 실험구에서 총 25속 47종(녹조류 30종, 규조류 9종, 남조류 6종, 편모조류 2종), 대조구에서 총 23속 45종(녹조류 26종, 규조류 10종, 남조류 7종, 편모조류 2종)의 식물플랑크톤이 각각 확인되었다. 식물플랑크톤 기능그룹(PFG)은 총 19개(B, C, D, F, G, H1, J, K, Lo, M, MP, N, P, S1, $T_B$, $W_0$, X1, X2, Y)로 확인되었으며, 실험구와 대조구에서는 H1을 제외하고 18개의 PFG가 모두 출현하였다. 생체 부피(biovolume)에 기초했을 때 $W_0$, J 및 M 그룹은 원수만 주입된 대조구와 비교하여 원수와 퇴적물이 주입된 실험구에서 영양염의 내부순환(internal cycling)의 영향으로 더 높은 생체부피를 보이면서 뚜렷한 천이 양상을 나타내었다. PCA 분석 결과에 기초할 때 PFG의 천이 양상은 영양염, 수온 및 조도 등의 주요 환경인자들과의 상관성에 따라 뚜렷한 차이를 나타내었다. 결론적으로 본 연구에서 PFG를 적용하여 주어진 환경조건에서 식물플랑크톤 적응 및 천이(성장) 특성을 확인할 수 있었으며, PFG는 향후 국내 수계의 식물플랑크톤 천이를 이해하기 위한 새로운 방법이 될 수 있을 것으로 생각된다.

• 한국유기농업학회지
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• 제25권2호
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• pp.373-386
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• 2017

본 시험은 전국의 광역친환경 농업단지 중에서 모범적으로 친환경 농업을 실천하고 있는 3곳(장흥, 순천, 옥천)의 벼 농가를 대상으로 시기별로 양분 분석을 하였다. 장흥지역 농가만이 경축자원 순환농업을 실천하고 있는 것으로 관찰되었다. 광역친환경 벼 농업단지의 3~9월 사이의 시기별 토양 pH는 5.5~6.7의 분포를 보였고, EC는 $0.4{\sim}1.0dS\;m^{-1}$으로 나타났다. 시기별 토양 전질소는 순천 농가에서 높았고 칼륨은 옥천 농가에서 낮은 수준을 보였다. 시기별 토양 암모늄태 질소는 봄철 비료 시용으로 6월 전후에 상승하였고, 장흥 농가에서 가장 높은 수준을 보이다가 이후 크게 감소하였다. 수량과 수확지수가 가장 높았던 옥천 농가에서 ha당 연간 조수입이 다른 농가보다 세배 가까이 높은 수준을 보였다. 비교적 많은 비료를 투입한 옥천 농가에서 시기별 양분수지가 높았고, 순천 농가의 ha당 칼륨 수지는 9월에 약 -60 kg까지 관찰되어 칼륨 결핍에 따른 생산성 저하에 일부 영향을 주었을 것으로 추정되었다. 시기별 전질소와 인산수지는 장흥 농가에서 가장 낮은 수준을 보였고 벼 낟알의 질소이용효율도 가장 높아서 광역친환경 농업단지의 추천할 만한 사례 농가로 평가되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10847-10853
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• 2015

Background: Lapatinib, a dual tyrosine kinase inhibitor that interrupts the epidermal growth factor receptor (EGFR) and HER2/neu pathways, has been indicated to have significant efficacy in treating HER2-positive breast cancer. However, acquired drug resistance has become a very serious clinical problem that hampers the use of this agent. In this study, we aimed to screen small molecule drugs that might reverse lapatinib-resistance of breast cancer by exploring differentially expressed genes (DEGs) via a bioinformatics method. Materials and Methods: We downloaded the gene expression profile of BT474-J4 (acquired lapatinib-resistant) and BT474 (lapatinib-sensitive) cell lines from the Gene Expression Omnibus (GEO) database and selected differentially expressed genes (DEGs) using dChip software. Then, gene ontology and pathway enrichment analyses were performed with the DAVID database. Finally, a connectivity map was utilized for predicting potential chemicals that reverse lapatinib-resistance. Results: A total of 1, 657 DEGs were obtained. These DEGs were enriched in 10 pathways, including cell cycling, regulation of actin cytoskeleton and focal adhesion associate examples. In addition, several small molecules were screened as the potential therapeutic agents capable of overcoming lapatinib-resistance. Conclusions: The results of our analysis provided a novel strategy for investigating the mechanism of lapatinib-resistance and identifying potential small molecule drugs for breast cancer treatment.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제7권1호
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• pp.61-73
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• 2004

목 적: 담즙울체를 포함한 간기능 이상은 총정맥영양법을 시행받는 환자에게서 자주 나타나는 합병증 중 하나이며 이를 해소하기 위한 방법의 하나로서 주기성 정맥영양법이 있으나 소아에서는 이에 대한 연구가 미흡한 상태이다. 본 연구에서는 소아에서 총정맥영양법의 합병증으로 생긴 간기능 이상의 발생초기에 주기성 정맥영양법을 시행하여 그 유용성을 평가하고자 하였다. 방 법: 연구대상은 미숙아를 제외한 유소아로 총정맥영양법에 의한 담즙울체 및 간효소치의 증가를 보이고 혈역학적, 대사적으로 안정되었으며 주기성 정맥영양법이 간기능 이상을 보이는 초기에 시도되었던 12례의 환아들이었다. 주기성 정맥영양법의 중단시간은 3개월 미만에서는 2시간 이하, 그 이상에서는 4시간으로 하였다. 환아들의 의무기록지를 후향적으로 검토하였고 진단명, 지속성과 주기성 정맥영양법의 시행기간과 방법, 대사성 혹은 감염성 합병증 유무, 병행된 경관영양유무 및 정맥영양법을 시행하기 전후의 혈청 총 빌리루빈과 간효소치의 변화를 비교하였다. 결 과: 1) 환아들의 정중연령(median age)은 주기성 정맥영양법 시작시 4세(생후 2개월에서 17세)였고 기저질환은 만성가성장폐색증 4례를 비롯하여 다양하였다. 2) 동일 예에서는 대부분에서 투여된 단백과 포도당, 지방의 투여량에 차이가 없었다(p<0.05). 주기성 정맥영양법을 시행하는 동안 1례에서 잘 조절되지 않는 고혈당이 나타나 포도당의 감량이 필요하였으나 다른 대사성 및 정맥관의 감염과 관련된 합병증은 없었다. 3) 혈청 총빌리루빈은 총 5례에서 상승하였고 주기성 정맥영양법 시행 후 전 증례에서 정상화되었다(p<0.05). 주기성으로 전환시 혈청 빌리루빈은 3.6 mg/dL였으며 정상화된 때까지 걸린 기간은 8일이었다. 4) AST/ALT의 상승은 12례에서 모두 관찰되었고 주기성 정맥영양법 시행 후 전례에서 정상화되었다. 주기성으로 전환시 혈청 ALT는 200 IU/L였으며 주기성 정맥영양 시행 후 정상화된 때까지 걸린 기간은 34일이었다. 결 론: 저자들은 장기간의 총정맥영양법으로 인해 발생한 간기능 이상을 보인 유소아에서 주기성 정맥영양법으로의 조기전환이 효과적인 치료적 대안이 될 수 있으며 부작용이 적은 안전한 방법임을 관찰하였다. 향후 주기성 정맥영양법은 총정맥영양을 시행받는 소아들에서 간기능 이상을 방지하고 삶의 질을 높이기 위한 일차적인 방법으로 더욱 연구되어야 할 것으로 생각된다.

• 대한약침학회지
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• 제18권2호
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• pp.7-18
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• 2015

Objectives: Lawsone (1,4 naphthoquinone) is a non redox cycling compound that can be catalyzed by DT diaphorase (DTD) into 1,2,4-trihydroxynaphthalene (THN), which can generate reactive oxygen species by auto oxidation. The purpose of this study was to evaluate the toxicity of the phytomarker 1,4 naphthoquinone and its metabolite THN by using the molecular docking program AutoDock 4. Methods: The 3D structure of ligands such as hydrogen peroxide ($H_2O_2$), nitric oxide synthase (NOS), catalase (CAT), glutathione (GSH), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH) and nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) were drawn using hyperchem drawing tools and minimizing the energy of all pdb files with the help of hyperchem by $MM^+$ followed by a semi-empirical (PM3) method. The docking process was studied with ligand molecules to identify suitable dockings at protein binding sites through annealing and genetic simulation algorithms. The program auto dock tools (ADT) was released as an extension suite to the python molecular viewer used to prepare proteins and ligands. Grids centered on active sites were obtained with spacings of $54{\times}55{\times}56$, and a grid spacing of 0.503 was calculated. Comparisons of Global and Local Search Methods in Drug Docking were adopted to determine parameters; a maximum number of 250,000 energy evaluations, a maximum number of generations of 27,000, and mutation and crossover rates of 0.02 and 0.8 were used. The number of docking runs was set to 10. Results: Lawsone and THN can be considered to efficiently bind with NOS, CAT, GSH, GR, G6PDH and NADPH, which has been confirmed through hydrogen bond affinity with the respective amino acids. Conclusion: Naphthoquinone derivatives of lawsone, which can be metabolized into THN by a catalyst DTD, were examined. Lawsone and THN were found to be identically potent molecules for their affinities for selected proteins.