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Pharmacokinetic Study of Pyrazinamide Related to the Mechanism of the Renal Excretion (Pyrazinamide의 신배설기전에 관한 약동학적 연구)

  • Choi, Eung-Sang;Shin, Sang-Goo;Lee, Sun-Hee;Choi, Cheol-Hee;Kim, Yong-Sik;Lim, Jung-Kyoo;Park, Chan-Woong
    • The Korean Journal of Pharmacology
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    • v.23 no.1
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    • pp.1-7
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    • 1987
  • The renal handling and tissue distribution of pyrazinamide were studied after administration of single dose intravenous injection for 15 min or constant infusion in New Zealand White rabbits. Peak pyrazinamide serum concentration ranged from 57.3 to $105.0{\mu}g/ml$ ($mean{\pm}SD;83.0{\pm}17.8$). The mean half-life of the a phase was $0.143{\pm}0.047$ hr while the ${\beta}$ phase ranged from 1.66 to 3.25 hr($mean{\pm}SD;2.38{\pm}0.57$). The mean steady-state volume of distribution in non-compartmental model was $0.935{\pm}0.362\;L/kg$ Excretion ratio of pyrazinamide was dramatically reduced from 1.02 to 0.30 when unbound serum pyrazinamide concentration was increased from 6.04 to $60.9\;{\mu}g/ml$. The urine flow dependency of renal clearance of pyrazinamide was demonstrated in steady-state serum concentration. The tissue/serum concentration ratio of pyrazinamide was highest in kidney and lowest in skeletal muscle among the tissues examined. The results suggested that a large fraction of pyrazinamide filtered by glomerulus and secreted by renal tubule was reabsorbed and this tubular reabsorption of pyrazinamide might be greatly influenced by urine flow.

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Clove Characteristics of Bulbs of Introduced Garlic Strains (도입(導入) 마늘구(球)의 인편(鱗片) 특성(特性))

  • Lee, Woo Sung;Lee, Ha Yoon
    • Current Research on Agriculture and Life Sciences
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    • v.9
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    • pp.1-9
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    • 1991
  • Bulbs of two introduced garlic cultivars, Sanghae-joseng and Spain strain, were purchased in a market and anatomical characteristics of them were investigated according to the weight of individual bulbs. The materials used were planted and grown in the field. Bulbs harvested were classified according to weight and their anatomical characteristics were recorded. 1. In both cultivars the larger the bulb, the more flat the bulb shape. 2. The bigger the bulbs were, the larger the number of cloves per bulb was, Spain strain had more cloves per bulb than Sanghae-joseng in the same size bulbs. 3. In Sanghae-joseng, more cloves were formed in outer layer than in inner layer in the bulbs produced in 1990. But more cloves were formed in inner layer in the bulbs of 35 g or smaller produced in 1991. In Spain strain, more cloves were formed in inner layer in the bulbs of 40 g or more produced in 1990. But more cloves were formed in outer layer in the bulbs produced in 1991 regardless of bulb size. In both cultivars, the number of primary cloves and total number of cloves was very small in both cultivars, but it was a little bigger in Sanghae-joseng than in spain strain. 5. In the bulbs produced in both 1990 and 1991 of Spain strain and in bulbs produced in 1990 of Sanghae-joseng weight of cloves in outer layer was significantly heavier than that in outer layer, the trend was not conspicuous in 1991 produce of Sanghae-joseng. 6. In both cultivars clove size range was narrow in small bulbs and wide in large bulbs. In distribution of cloves in size, medium ones were most frequent, and the frequency of large cloves increased with increased size of bulbs. Sanghae-joseng produced larger cloves than Spain strain in the same size cloves.

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Changes in Protease and Formonitrogen of Salted Fish and Shellfish (1) (젓갈 성숙과정(成熟過程)에 있어 Protease 및 Formonitrogen의 변화(變化)에 관(關)한 연구(硏究)(1))

  • Suh, Myung-Jah
    • Journal of Nutrition and Health
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    • v.6 no.2
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    • pp.45-56
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    • 1973
  • Salted fish and shellfish have been widely used in Korea from olden times as side-dishes, although the processes and methods of pickling have varied depending on localities. The common raw materials for these subsidiary food articles include anchovy, shrimp, yellow corvina, oysters, octopus, top-shell, shellfish, pollack roe and pollack intestines. It must be pointed out here, however, that the salted stuffs now marketed locally are highly unscientific and unsanitary in the way they are processed and sold, and this has prompted this writer to undertake a study on these native food articles. The following findings have been obtained from this study on the changes in Formonitrogen and Protease Activity, effected by the density of salt and the degree of storing temperature, of the pickles of cedfish gills, codfish intestines, pollack intestines, shellfish, oysters, cuttle fish and octopus. 1) Codfish Gills The Protease Activity of the pickled codfish gills was greater in the groups of lesser doses of salt and higher degrees of storing temperature. The same was true in the case of Formonifrogen, too. The Formonitrogen of the pickled codfish gills was larger in the groups of lesser salt and higher teimperature. 2) Codfish Intestines The amount of Formonitrogen of the pickled codfiah intestines became greater, as time went by, in the groups of lesser salt than those of larger doses of salt, with the speed of its formation getting faster as the storing temperature rose from $5^{\circ}C$ to $15^{\circ}C$ and $30^{\circ}C$. The Protease Activity was also greater in the groups of lesser salt and higher temperature. The group, stored at 10% salt and $30^{\circ}C$, rotted in five days. 3) Pollack Intestines The amount of Formonitrogen of the pickled pollack intestines was greater in the groups of lesser amount of salt and higher degrees of storing temperature. The Protease Activity of the pickled pollack intestines began decreasing from the 11th day after the pickling in the groups stored at colmparatively high degrees of temperature$(15^{\circ}C-30^{\circ}C)$, while that of the group stored at $5^{\circ}C$ kept rising. The effects of the amount of salt were little. The group stored at 15% salt and $30^{\circ}C$ started rotting on the 13th day while that stored at $30^{\circ}C$ decayed on the 7th day. The group stored at 20% salt and $30^{\circ}C$ rotted on the 9th day. 4) Oysters The amount of Formonitrogen of the pickled oysters became greater as the storing temperature rose and the doses of salt were lowered. The Protease Activity was not affected at any measurable degree by the density of salt in the group stored at $5^{\circ}C$, but became greater as the storing temperature rose to $15^{\circ}C$ and $30^{\circ}C$. The group stored at 10% salt and $30^{\circ}C$ rotted on the 5th day while that stored at 20% salt and $30^{\circ}C$ on the 13th day. 5) Shellfish The amount of Formonitrogen of the pickled shellfish became greater, as time went by, it the groups of lower consistency of salt than the groups of higher density of salt, although the decay of the former groups was faster than the latter groups. The density of salt best fitted for the pickling appeared to be about 20% with the storing temperature to be $15^{\circ}C$, at which the pickled stuff became most tasty on the 7th day. The oysters stored in three groups at $5^{\circ}C$, $15^{\circ}C$ and $30^{\circ}C$ respectively showed the greatest Protease Activity alike at 0% of salt, but the activity declined as the density of salt increased. The Protease Activity of each group rose for the first 11 days after the pickling, but began declining from the 13th day onward, with the groups of higher temperature retaining higher Protease Activity than the groups of lower temperature. 6) Cuttlefish Both the amount of Formonitrogen and the degree of Protease Activity of the pickled cuttlefish were greater in the groups of lower density of salt and higher degree of storing temperature. The oysters pickled at 10% salt and $15^{\circ}C$ degenerated on the 13th day while that of 10% salt and $30^{\circ}C$ deteriorated on the 7th day. 7) Octopus Both the Formonitrogen and the Protease Activity of the pickled octopus were greater in the groups of lower density of salt, but as time went by, the Protease Activity in all groups dwindled after a climbing. In general, the Formonitrogen and the Protease Activity of the pickled oysters became greater as the storing temperature got higher. One group stored at 10% salt and $15^{\circ}C$ rotted in 13days while another group stored at $30^{\circ}C$ decayed in 7 days.

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Evaluation of the radiopacity and cytotoxicity of resinous root canal sealers (레진계 근관충전실러의 방사선 불투과성 및 세포 독성에 대한 평가)

  • Kim, Chang-Kyu;Ryu, Hyun-Wook;Chang, Hoon-Sang;Lee, Byung-Do;Min, Kyung-San;Hong, Chan-Ui
    • Restorative Dentistry and Endodontics
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    • v.32 no.5
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    • pp.419-425
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    • 2007
  • The aim of this study was to evaluate the radiopacity and cytotoxicity of three resin-based (AH 26, EZ fill and AD Seal), a zinc oxide-eugenol-based (ZOB Seal), and a calcium hydroxide-based (Sealapex) root canal sealers. Specimens, 10 mm in diameter and 1 mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the Scion image software. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of each material was determined in immortalized human periodontal ligament (IPDL) cells. The results demonstrated that EZ fill was the most radiopaque sealer, while Sealapex was the least radiopaque (p < 0.05). AH 26, AD Seal and ZOB Seal presented intermediate radiopacity values. All the materials evaluated, except for Sealapex, presented the minimum radiopacity required by ISO standards. The cell viabilities of resin-based root canal sealers were statistically higher than that of other type of root canal sealers through the all experimental time. Further, EZ fill showed statistically lower cell viability in 24 and 48 hours compared to AD Seal and in 72 hours compared to all other resin-based root canal sealers. However, there was no correlation between the radiopacity and cytotoxicity of three resin-based root canals sealers (p > 0.05). These results indicate that resin-based root canal sealer is more biocompatible and has advantage in terms of radiopacity.

Analysis of sodium content of representative Korean foods high in sodium from home meal, foodservice, and restaurants (가정식, 급식, 외식 고나트륨 한식 대표 음식의 나트륨 함량 분석)

  • Jiang, Lin;Lee, Yeon-Kyung
    • Journal of Nutrition and Health
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    • v.50 no.6
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    • pp.655-663
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    • 2017
  • Purpose: The aim of this study was to analyze the sodium contents of representative Korean foods high in sodium and develop them as a fundamental resource for setting the standard amount of sodium in foods. Methods: A total of 480 representative Korean foods high in sodium were collected from households, foodservice establishments, and restaurants in four regions (Capital region, Chungcheong & Gangwon Province, Gyeongsang province, and Jeolla province) and analyzed their sodium content. Results: The sodium content (per 100 g) of food categorized by food group was highest in kimchi and braised food (p < 0.001), and the sodium content of each food was highest in stir-fried anchovies (1,896.6 mg) followed by braised burdock and lotus roots (820.6 mg) and picked onions (809.3 mg) (p < 0.001). However, the sodium content per portion of food was highest in steamed short ribs (1,429.6 mg) from home meals (p < 0.001), Bibimbap, (1,074.1 mg) and steamed short ribs (838.8 mg) from foodservice (p < 0.001), spicy beef soup (3,273.2 mg), and pollack stew (2,930.2 mg) from restaurants (p < 0.001). The sodium content per 100 g and portion of soups and stews from restaurants was significantly higher than that of home meals and foodservice meals (p < 0.001), and the sodium content per portion of steam dishes and stir-fried dishes from home meals was significantly higher than that of foodservice meals (p < 0.05). Conclusions: These results suggest that developing and using low-sodium soybean sauces & soybean paste and kimchi is needed in an effort to reduce sodium intake. In addition, determination of the sodium contents of representative Korean foods known to be high in sodium is expected to be useful in establishing guidelines for reduction of sodium content.

Early Changes after Death of Plaice, Paralichthys olivaceus Muscle -6. Effect of Killing Methods on Morphological Changes of Myofibrills and Histological Changes of Muscle- (넙치 (Paralichthys olivaceus)육의 사후조기변화 -6. 치사 방법이 근원섬유의 형태학적 및 육의 조직학적인 변화에 미치는 영향-)

  • CHO Young-Je;LEE Nam-Geoul;KIM Yuck-Yong;KIM Jae-Hyun;LEE Keun-Woo;KIM Geon-Bae;CHOI Young-Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.4
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    • pp.327-334
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    • 1994
  • This study was undertaken to clarify the effect of killing methods on the morphological and histological changes of plaice, Paralichthys olivaceus muscle at early stage after killing. Killed samples by the three different methods were stored at $5^{\circ}$, and the changes in breaking strength of muscle, morphological observation of myofibrils and histological observation of extracellular spaces through storage were monitored. Samples killed by electrifying in sea water showed the maximum value of breakin strength immediately after killing and then it dropped significantly(p<0.05) until 2.5hrs passed. Breaking strength of samples killed by spiking at the head instantly and dipping in sea water including anesthetic rose steadily over 10hrs and 15hrs after killing, respectively. In myofibrills prepared from dorsal muscles immediately after spiking at the head instantly, A-band, H-band, I-band, and Z-line in sarcomere were clearly distinguishable each other. Due to muscle contraction by electrical stimulation, it was impossible to distinguish H-band from I-band observed in sarcomere immediately after killing for samples killed by electrifying. But, in the cases of samples killed by spiking and dipping, H-band could be observed dimly until 10hrs and 15hrs storage. No extracellular space was observed among muscle cells immediately after spiking at the head instantly. Samples killed by spiking at the head instantly and dipping in sea water including anesthetic showed extracellular spaces among all muscle cells after 15hrs and 25hrs storage, respectively. The other hand, samples killed by electrifying in sea water (110V, 30sec.) showed a few extracellular spaces immediately after killing and then it showed extracellular spaces among all muscle cells after 2.5hrs storage.

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Effects of High Molecular Hardwood Lignin on Anaerobic Digestion at Different Temperatures and Sludge Concentrations (혐기성 소화에 미치는 온도와 슬러지의 농도별 고분자 활엽수 리그닌의 영향)

  • Yin, Cheng-Ri;Seo, Dong-Il;Lee, Sung-Taik;Jin, Yin-Shu
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.12
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    • pp.2197-2204
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    • 2000
  • Lignin is a major component of wastewater generated in the chemical processing of wood. Because it is recalcitrant, it inhibits biological treatment of wastewater of pulp manufacturing, especially high concentration of lignin may inhibit the anaerobic digestion. The objective of this study was to evaluate the toxicity of high molecular hardwood lignin (lignosulfonate, MW $\geq$ 20,000) on aceticlastic methanogens in the batch reactors at different temperatures with different sludge concentrations, using anaerobic serum bottles. The hardwood lignin was found to inhibit anaerobic conversion of acetate to methane and carbon dioxide, shown with a long lag-phase before methanogenesis started. The methanogens assumed not to be able to acclimate to the lignin were found to be acclimated slowly in the batch experiments, finally reaching non-toxic levels in which methane production could start. The hardwood lignin was found not to be bacteriocidal but bacteriostatic to aceticlastic methanogens. Hardwood lignin(lignosulfonate) at 1.3, 2.6, and 3.9%(w/w) inhibited the acetateutilizing methanogens of anaerobic digester sludge by 14.5, 17.8, 21.1 days(in noninhibitory condition it took 10 days) to produce the same amount of methane. The inhibitory effect of lignin was examined at temperature ranges of $30^{\circ}C$ to $50^{\circ}C$. When 2.6% of lignin was contained in wastewater, methane production was highest at $30^{\circ}C$ during initial 8 days. At $4^{\circ}C$, methane production rapidly increased after 12 days of digestion, the value became higher than that at $30^{\circ}C$ after 14 days. However, the methane production was completely inhibited during whole digestion period at $50^{\circ}C$. High ratio of lignin concentration to initial anaerobic sludge concentration gave tolerance to the inhibition. In this experiment, high molecular hardwood lignin was not degraded and decolorized.

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Evaluation of Dietary Intake of Korean School-aged Children from Low-income Families by Comparing with the Korean Food Guide: Analysis of the Data from the 2001 National Health and Nutrition Survey (식사구성안을 이용한 저소득층 학령기 어린이의 식생활 평가: 2001년 국민건강.영양조사 자료 분석)

  • Shim, Jae-Eun;Yoon, Ji-Hyun;Lee, Ki-Won;Kwon, Soo-Youn
    • Journal of Nutrition and Health
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    • v.42 no.8
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    • pp.691-701
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    • 2009
  • This study was conducted to evaluate the dietary quality of school-aged children from low-income families in comparison with those from higher income families. The socio-demographic and anthropometric data and one day 24-hour recall dietary intake data of 1,782 school-aged children were used for analysis from the data of 2001 National Health and Nutrition Survey. The children whose family had monthly household income 120% or lower than the 2001 Minimum Cost of Living were classified into Low Income Group (LIG), and the others were classified into Higher Income Group (HIG). The consumption levels of the food groups of the Korean Food Guide as well as the general nutritional status based on their weight and height, energy and nutrient intake were compared between LIG and HIG in elementary (n = 989), middle (n = 432), and high (n = 361) school-aged children, respectively. LIG tended to have lower weight, height, and BMI than HIG, but the difference was significant only in the weight and the BMI of the middle school-aged children. The energy intake of LIG didn't differ from that of HIG, but LIG consumed a higher percentage of energy from carbohydrate and a lower percentage of energy from fat than HIG. Some micronutrient intake was lower in LIG than HIG in case of the elementary and the middle school-aged children. The average numbers of servings of milk dairy products and fruits consumed by LIG were significantly lower than those consumed by HIG in the elementary and the middle school-aged children, and they were less than the respective recommended serving. The average number of servings of meat fish egg beans consumed by LIG was lower than that consumed by HIG in the middle and the high school-aged children, and it was also less than the recommended serving. The results suggested that the diet of children from low-income families should be intervened by strategies different from the other children in order to improve the consumption level of milk dairy products, fruits, and meat fish egg beans.

Effect of Ischemic Preconditioning on the Oxygen Free Radical Production in the Post-ischemic Reperfused Heart

  • Park, Jong-Wan;Kim, Young-Hoon;Uhm, Chang-Sub;Bae, Jae-Moon;Park, Chan-Woong;Kim, Myung-Suk
    • The Korean Journal of Pharmacology
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    • v.30 no.3
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    • pp.321-330
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    • 1994
  • The protective effect of 'ischemic preconditioning (PC)' on ischemia-reperfusion injury of heart has been reported in various animal species, but without known mechanisms in detail. In an attempt to investigate the cardioprotective mechanism of PC, we examined the effects of PC on the myocardial oxidative injuries and the oxygen free radical production in the ischemia-reperfusion model of isolated Langendorff preparations of rat hearts. PC was performed with three episodes of 5 min ischemia and 5 min reperfusion before the induction of prolonged ischemia (30 min)-reperfusion(20 min). PC prevented the depression of cardiac function (left ventricular pressure x heart rate) observed in the ischemic-reperfused heart, and reduced the release of lactate dehydrogenase during the reperfusion period. On electron microscopic pictures, myocardial ultrastructures were relatively well preserved in PC hearts as compared with non-PC ischemic-reperfused hearts. In PC hearts, lipid peroxidation of myocardial tissue as estimated from malondialdehyde production was markedly reduced. PC did not affect the activity of xanthine oxidase which is a major source of oxygen radicals in the ischemic rat hearts, but the myocardial content of hypoxanthine (a substrate for xanthine oxidase) was much lower in PC hearts. It is suggested from these results that PC brings about significant myocardial protection in ischemic-reperfused heart and this effect may be related to the suppression of oxygen free radical reactions.

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Effects of the Draronis sanguis on Antioxidation and MMP-1 Expression in Human Dermal Fibroblast (혈갈(Draconis Sanguis)의 항산화와 사람섬유아세포에서 MMP-1 발현저해 효과)

  • Sim Gwan Sub;Kim Jin Hui;Kim Jin Hwa;Lee Dong Hawn;Park Sung Min;Lee Bum Chun;Pyo Hyeong Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.4 s.48
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    • pp.439-444
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    • 2004
  • UV irradiation produces free radicals and related reactive oxygen species (ROS), and these are injury to all most of organisms of skin cells and extracellular matrix (ECM). In addition, free radicals and ROS stimulate the overexpression of matrix metalloproteinases (MMPs) that can degrade most components of ECM such as collagen. Since collagen constitutes almost of skin connective tissue, their disarrangement causes wrinkle formation and droop of skin. Therefore, scavenging activity on free radicals, ROS and suppression of MMP-1 is expected to prevent skin photoaging. In this study, to investigate the relationship between photoaging and Draconis sanguis, we examined the effects of antioxidant, in vitro MMP inhibition and expression of UVA-induced MMP-1 in human dermal fibroblasts. Draconis sanguis was found to show scavenging activities of radicals and ROS with the $IC_{50}$ values of $183{\;}{\mu}g/mL$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and $30{\;}{\mu}g/mL$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Draconis sunguis inhibited the activities of MMP-1 in a does-dependent manner and the $IC_{50}$ value calculated from semi-log plots was $200{\;}{\mu}g/mL$. Also, UVA induced MMP expression was reduced $74\%$ by treatment with Draconis sanguis, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore Draconis sanguis was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that Draconis sanguis may act as an anti-photoaging agent by antioxidation and reducing UVA-induced MMP-1 production.