• 제목/요약/키워드: 4-period development

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왕사슴벌레(Dorcus hopei)의 대량사육 기술개발을 위한 생태특성 조사 (Ecological Characteristics of Dorcus hopei(E Saunders) for the Development of Mass-rearing Technique in Korea)

  • 김철학;이준석;정근;박규택
    • 한국응용곤충학회지
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    • 제43권2호
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    • pp.135-141
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    • 2004
  • 본 연구에서는 왕사슴벌레의 국내 분포지가 재검토되고 이들의 대량사육기술 개발을 위한 생태적 특성이 조사되었다. 산란수를 최대화하기 위해서는 우화 후6개월이 지난 암, 수 성충에게 2주 이상의 교미기간이 허용되어야 하며 성비는 암:수 = 2:1이 이상적이었다. 산란수는 암컷 한 마리 당 최대 27.3개이고 알은 한 개씩 산란된다. 산란전기간은 평균 147.3일, 산란기간은 최소 120일 이었으며 난기간은 평균 15.6일이었다. 산란상은 직경 15cm${\times}$길이 15cm인 산란목이 3-4개 들어 있는 55${\times}$40${\times}$35cm크기의 산란상이 이상적이었다. 산란목으로는 상수리나무가 선호되었다. 사육실조건($25^{\circ}C$, 75% R.H., 16L:8D)에서 1령기간은 24.1일,2령은 29.8일, 3령에는 131.2일이 소요되었으며 용기간은 28.9일이 소요되었다. 성충수명은 35개월 이상이었다. 산란수를 증가시키기 위해서는 최소 3-4개월의 휴면기간이 필요한 것으로 밝혀졌다.

동사리, Odontobutis platycephala (Iwata et jeon) 수컷의 생식주기에 따른 정소 발달과 혈중 생식소 스테로이드의 변화 (Testicular Development and Serum Levels of Gonadal Steroids Hormone during the Annual Reproductive Cycle of the Male Koran Dark Sleeper, Odontobutis platycephala (Iwata et Jeon))

  • 이원교;양석우
    • 한국양식학회지
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    • 제11권4호
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    • pp.475-485
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    • 1998
  • 동사리, Odontobutis platycephala 수컷의 명확한 생식주기를 밝히고자 1995년 12월부터 1997년 11월까지 생식소 중량지수(gonadosomatic index, GSI), 정소의 발달양상 및 혈중 성스테로이드 호르몬 변화양상을 조사하였다. 정소의 모양은 7월부터 10월까지는 콩깍지 모양이었는데, 11월부터는 후반부가 비대해져 올챙이와 유사한 형태였다. GSI는 7월부터 9월까지 0.14~0.18였으며, 10월에 $0.43{\pm}0.04$로 증가하여 2월까지 유의한 변화를 보이지 않았으나, 3월에는 $0.52{\pm}0.09$로 증가하였으며, 5월까지는 비슷한 수준에 머물렀다가, 6월에는 $0.28{\pm}0.05$로 낮아졌다. 생식주기에 따른 정소의 생식세포 발달은 전반부, 경계부, 후반부에서 다른 양상을 보였다. 7월의 대부분 개체의 정소는 세정관이 없었으며, 정자형성과정이 아직 시작되지 않은 상태로 정원세포들만을 볼 수 있었다. 전반부에서 정자형성단계의 세정관들은 8월에 출현하여 9월부터 12월까지는 80% 이상이었으며 1월부터 3월까지는 점차적으로, 4월에는 급격히 감소하여 6월에는 나타나지 않았다. 정자완성단계 세정관은 12월부터 출현하여 3월까지 점차적으로, 4월에 80%로 급격히 증가한 후 6월에 90%로 년 중 최고 수준에 이르렀다. 그러나 정자방출후 세정관은 나타나지 않았으며, 정원세포들은 7월에 100%, 8월에 65% 였으나 나머지 기간에는 20%이하로 년 중 존재하였다. 경계부에서도 8월부터 정자형성단계 세정관이 출현한 후 매월 증가하여 11월에 82%에 이르렀으며 12월부터 감소하여 3월부터는 나타나지 않았다. 정자완성단계 세정관은 11월에 출현하여 2월까지는 18% 이하였으나, 3월부터 6월까지 29%~57%의 수준을 보였다. 정자방출후 세정관은 3월부터 6월까지 12%~25%로 나타났다. 정원세포는 8월에 85%, 9월에 67%, 10월에 35%, 11월에 10%로 감소하였으며, 1월부터는 점차적으로 증가하여 4월에 50%를 보인후 6월까지 다시 점차적으로 감소하였다. 후반부에서는 일부의 개체에서 수개의 정원세포를 지닌 세정관이 7월부터 출현하였다. 정자형성단계에 세정관은 8월에 80%, 9월에 85%였으나 10월부터는 급격히 감소하여 11월에서 2월까지는 10%이하였으며, 3월부터는 나타나지 않았다. 정자완성단계 세정관은 8월에 출현하여 10월부터 급격히 증가해 11월에는 75%에 이르렀고 12월에는 15%로 급격히 감소하여 3월까지 유의한 변화를 보이지 않았으며 4월부터는 나타나지 않았다. 정자방출후 세정관은 11월에 출현하여 12월에 82%로 급격히 증가한 후 6월까지 85%~95%로 높은 수준을 유지하였다. 정원세포들은 8월에 15%로 감소하였으며 10월부터 3월까지는 나타나지 않았다. 그러나 4월부터 다시 출현하여 6월까지 10%이하에 머물렀다. 혈중 testosterone (T)의 농도는 8월부터 완만하게 증가하여 11월에 $0.61{\pm}0.09 ng/m\ell$ 였으며, 12월부터는 $3.99{\pm}1.22 ng/m\ell$로 급격히 상승하여 3월까지 높은 수준을 유지했다. 그러나 4월에 $0.52{\pm}0.14ng/m\ell$로 감소하기 시작하였으며, 5월에 $0.17{\pm}0.13 ng/m\ell$로 낮아진 후 7월까지 유의한 변화는 보이지 않았다(P<0.05). $17\alpha$,$20\beta$-dihyd-roxy-4-pregnen-3-one ($17\alpha$,$20\beta$-P)은 12월에 $0.19{\pm}0.01 ng/m\ell$로 약간 감소하였으며, 4월까지는 유의한 변화는 보이지 않고, 5월에 $0.38{\pm}0.03 ng/m\ell$로 급격히 증가하여 peak에 달하였다. 6월에는 $0.19{\pm}0.04 ng/m\ell$로 낮아졌으며, 11월까지 유의한 변화는 보이지 않았다(P<0.05). 이상의 결과를 종합하면 동사리는 정소의 부위에 따라 각각 다른 발달양상을 보였다. 즉 전반부에는 7월이 정원세포증식기, 8월~11월은 성숙초기, 12월~3월은 성숙중기, 4월~5월은 성숙후기, 6월은 기능적 성숙기로 구분되었다. 경계부에서는 7월이 정원세포 증식기, 8월~10월은 성숙초기, 11월~2월은 성숙중기였으며, 3월~6월은 성숙후기, 기능적 성숙기, 후번식기의 특징이 동시에 출현하였다. 후반부에서는 7월이 정원세포증식기, 8월~9월은 성숙중기, 10월은 성숙후기, 11월은 기능적 성숙기, 12월~6월은 후번식기로 구분되었다. 이러한 생식주기의 변화는 성스테로이드 호르몬의 변화양상과 밀접한 관련이 있는 것으로 생각된다.

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Changes in Sex Steroid Hormones and Ovarian Development during Artificial Maturation of Female Eel, Anguilla japonica

  • Kim, Dae-Jung;Bae, Jun-Young;Kim, Eung-Oh
    • Animal cells and systems
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    • 제11권2호
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    • pp.117-124
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    • 2007
  • The present study demonstrates the changes in body weight (BW) and plasma sex steroid hormone profiles during artificial maturation induced by human chorionic gonadotropin (HCG) or salmon pituitary extract (SPE) injections in cultured eel, Anguilla japonica, kept in seawater for 3 months. In the weekly SPE-injected female group, BW was relatively stable during vitellogenesis. Following induction of vitellogenesis, females exhibited a rapid increase of BW, and the oocytes were observed to be in the migratory nucleus stage at the end of the experiment. Plasma testosterone (T) and $estradiol-17{\beta}$ ($E_2$) levels increased slightly during vitellogenesis and peaked at an average of 5.82 ng/mL and 4.76 ng/mL, respectively, at the end of the experiment. In the weekly control and HCG-injected female groups, BW slowly decreased during the experimental period, and the oocytes of the two groups were observed to be at the primary yolk globule stage. In the weekly HCG-injected female group, plasma T and $E_2$ levels increased slightly during vitellogenesis and decreased afterward. In the control female group, however, plasma T and $E_2$ levels were not altered during the experimental period. Furthermore, plasma $17{\alpha},20{\beta}-dihydroxy-4-pregnen-3-one$ (DHP) was not detected in all experimental groups. Fertility and hatching rates of SPE-injected females were significantly higher in those that ovulated 15 h after DHP injection than 18 h. These results indicate that long rearing in seawater increases responsiveness to SPE in ovarian maturation of the Japanese eel, resulting in shortened period from completion of vitellogenesis by sex steroid hormone production.

산후우울 사정을 위한 도구 개발 연구 (A Study on the Development of a Postpartum Depression Scale)

  • 배정이
    • 대한간호학회지
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    • 제27권3호
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    • pp.588-600
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    • 1997
  • Postpartum depression is one of the most serious problems in maternal health because it affects not only the mother but also her family. Postpartum depression disturbs the maternal-infant interaction and attachment. However, most postpartum depression patients ignore this problem and do not seek treatment. Many clinicians and researchers realiza there is a need to develop a postpartum depression scale. Thus, this study has been designed to development of a postpartum depression scale. Data were collected through a survey over a period of three months. Subjects who participated in the study were 167 Korean mothers in their postpartum period. The author used a convenience sampling method. The analysis of the data was done with SPSS PC/sup +/ for descriptive statistics, item analysis and factor analysis. Initially 62 items were generated from the interview data of eight postpartum depression patients and from a literature review. This preliminary scale was analyzed for reliability and validity. The results of this analysis are as follows. 1. Initially 62 items were analyzed through the Index of Content Validity(CVI) and 48 items were selected. 2. Seven factors were extracted through the principal component analysis, and these contributed 61% of the variance in the total score. Finally 46 items in the scale loaded .41∼ .84 on one of seven factors. 3. Each factor was labeled. Factor 1 was labeled 'emotional phenomena-emotional upset' and included 13 items, factor 2 was labeled' cognitive phenomena-self concept disturbance' and included seven items, factor 3 was labeled 'relationship to baby-negative feeling' and included six items, factor 4 was labeled 'relationship to baby- overload' and included eight items, factor 5 was labeled 'negative maternal identity' and included five items, factor 6 was labeled 'biophysiological phenomena-disturbance of physical functioning' and included four items, and factor 7 was labeled' interpersonal relationship phenomena-blamed others' and included three items. 4. Cronbach Coefficient Alpha for internal consistency was .95 for the total 46 items. Finally, the author suggests that this scale could be adequately applied in assessing the postpartum depression of mothers during the postpartum period. The results of this study can contribute to designing an appropriate postpartum depression prevention strategy.

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Optimal Timing and Duration of Cold Application for Breaking Diapause in Queens of the Bumblebee Bombus ignitus

  • Yoon, Hyung Joo;Lee, Kyeong Yong
    • International Journal of Industrial Entomology and Biomaterials
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    • 제28권2호
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    • pp.51-57
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    • 2014
  • Bumblebees are important pollinators of crops and wildflowers. The Korean native bumblebee, Bombus ignitus, undergoes one generation per year, and induction of artificial hibernation is essential for year-round rearing of the bumblebee. Keeping queens under cold treatment conditions for several mo is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how the timing and duration of chilling affect the artificial hibernation of B. ignitus queens. In the timing assessment, cold treatment was instituted at 12 d, 40 d, or 100 d after eclosion under a constant temperature of $5^{\circ}C$ and 80% humidity. The queens that entered cold treatment at 12 d after emergence evidenced the highest survival rates: 86.7% at two mo, 73.3% at three mo, and 46.4% at 4 mo. Survival rates were reduced under storage conditions at 12 d, 40 d, and 100 d after emergence. When queens were subjected to chilling at 8 d, 12 d, or 16 d after eclosion with constant 80% humidity, the queens stored at 12 d after eclosion exhibited the highest survival rates, which were 84.6 at one mo, 25.0% at two mo, and 7.9% at three mo. In regards to the duration of the cold period, the queens that hibernated for at least two mo evidenced optimal colony development rates. The rates of oviposition, colony foundation, and progeny-queen production of queens hibernated for two mo were 60.0%, 30.0%, and 13.3%, respectively. These values were 6.0 to 13.3 times higher than those in the queens that hibernated for 15 d. Therefore, a cold period of at least 2 mo applied 12 d after emergence were found to be the most favorable conditions for diapause break in B. ignitus queens.

학동기와 청소년기 정상 및 정신지체아 파악력 분석 (An Analysis of Grip Strength between Normal and Mentally Retarded subjects in Prepuberal period and Adolescence)

  • 라기용
    • The Journal of Korean Physical Therapy
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    • 제15권3호
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    • pp.91-109
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    • 2003
  • The grip strength is orthopedics department, the place where from rehabilitation medical science and industrial medical science and it evaluates the function of the hand certainly is the ability which is necessary. There is specially by an occupation rehabilitation of mentally retarded child and the evaluation of grip strength is one which is meaning. The samples for this study were 39 normal males and 59 normal females, 62 mentally retarded males, 33 mentally retarded females ranging in age from Prepubeal period to Adolescence. This study was used to measure grip strength BASELINE Hydraulic Hand Dynamometer manufactured by EEI(Fabrication Enterprises Incorporated)in USA. The objective of research from the Prepubeal period to Adolescence the normal and mentally retarded students comparison evaluates the grip strength and effective fine motor program of one's it makes with the fundamental data for a development and it does. The results are as follow : (1) The hand which the normal student and the schoolgirl of rises from grip strength measurement result of the Prepubeal period research object people 10.7Kg with the same left hand was each measured 10Kg with 9.8Kg with in. The grip strength of the mentally retarded schoolboys was the hand which rises 6Kg with the left hand 5.4Kg, the grip strength of the schoolgirls 6.5Kg with was identical. (2) The hand which the normal schoolboys and the schoolgirls rises from grip strength measurement result of the Adolescence research object people 29.6Kg, 20Kg the left hand to be was each measured 27.8Kg with 18.4Kg with. The hand which the mentally retarded schoolboys and the schoolgirls rises 13.1Kg, 11.3Kg the left hand to be was each measured 12.3Kg with 10.9Kg with. (3) Among the normal students $10.2\%$ (Prepubeal period $0.94\%$, Adolescence $15.2\%$) in Mentally Retarded students $17.9\%$ (Prepubeal period $0.77\%$, Adolescence $19.1\%$) left hand it was higher. (4) The grip strength of the students who attend school from the general family dwells were appeared higher than students residence at institution.

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한중환경하 타설된 구조체콘크리트의 결합재 종류별 관리재령 28일 설계기준강도 확보 기법 (The designed compressive strength assurance method to the concrete subjected to cold weather at 28 days)

  • 이영준;현승용;이상운;이정교;한민철;한천구
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2018년도 춘계 학술논문 발표대회
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    • pp.47-48
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    • 2018
  • The aim of the research is to suggest the compensating strength values depending on various managing periods of concrete based on the strength development model calculated with equivalent age method for OPC 100 % concrete. As a result, for 28 days of managing period, 6, and 3 MPa of compensating strength values were suggested when the temperatures were from 4 to 9℃, from 9 to 17℃, respectively. Additionally, for 42 days of managing period, 3MPa of compensating strength value was suggested when the temperature was from 4 to 10℃, and for 56 days of managing period, 3 MPa of compensating strength value was suggested when the temperature was from 4 to 5℃. Furthermore, for 28, 42, 56, and 91 days of managing periods, any compensating strength values were needed when the temperature were higher than 17, 10, 5, and 4℃, respectively.

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일부 치위생학과의 LINC+(2019~2021) 사업 성과에 관한 연구 (A Study on the Performance Analysis of LINC+(2019~2021) Program in Dental Hygiene Department)

  • 김창숙
    • 농촌의학ㆍ지역보건
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    • 제47권4호
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    • pp.275-282
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    • 2022
  • Objective: This study intends to analyze the performance of LINC+ program for three years of the dental hygiene department at Ulsan college and to present the direction for the operation of the LINC 3.0 project to be conducted for 6 years. Methods: A survey was conducted on 87 human resources officers participating in the LINC+ project of dental hygiene department from July 2021 to August 30, 2021, and the IBM SPSS Statistics 24.0 program was used for data analysis. Results: In terms of the suitability of the curriculum, operation of clinical practice averaged 4.82 points and omnibus courses averaged 4.77 points, and it was identified that OJT course, clinical practice, and educational environment make effects on job adaptation period, education and training period, and the reduction of education and training costs(p<0.05). Conclusion: In order to produce graduates who are highly adaptable in the field, participation of industries in the development and operation of the curriculum must be expanded and jointly operated, and it was possible to confirm the importance of the same educational environment as the industry to shorten the job adjustment period.

성장초기의 격일급이가 육계 수컷의 후기 보상성장과 장기발달에 미치는 효과 (Effects of Skip-a-day Feeding Program in Early Growing Stage on Compensatory Growth and Development of Visceral Organs Following Realimentation in Male Broiler Chicks)

  • 유동조;이봉덕;이수기;한성욱;임재삼;서옥석
    • 한국가금학회지
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    • 제23권4호
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    • pp.185-191
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    • 1996
  • A feeding trial was conducted to investigate the effect of skip-a-day feeding program in early growing stage of male broiler chicks on the compensatory growth, and development of visceral organs during the later realimentation period. Day-old 328 male broiler chickens( Avian x Avian) were divided into control(ad libitum feeding) and skip-a-day feeding(limited feeding) treatments. Each treatment was consisted of 4 pens( replicates) of 41 birds each. Chickens on the skip-a-day program were fasted every other day during the second wk of age(8~15 d of age). During the starter period(0~4 wk), chickens were fed a commercial crumble diet. A commercial pellet diet was fed to chickens during the finishing period( 5~8 wk). Chickens were group-weighed every week, and one bird was selected from each pen to measure carcass characteristics. The skip-a-day program delayed the growth rate of male broilers up to 4 wk of age(P

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박사르®정 4 밀리그램(라시디핀 4 mg)에 대한 라니디엠®정 4 밀리그램의 생물학적동등성 (Bioequivalence of LANIDIEM® Tablet 4 mg to Vaxar® Tablet 4 mg(Lacidipine 4 mg))

  • 이윤영;김혜진;나숙희;조경희;장문선;박영준;이희주
    • Journal of Pharmaceutical Investigation
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    • 제40권2호
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    • pp.125-131
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    • 2010
  • A bioequivalence study of LANIDIEM$^{(R)}$ tablet 4 mg (Samil. Co., Ltd.) to Vaxar$^{(R)}$ tablet 4 mg (GlaxoSmithKline Co., Ltd.) was conducted according to the guidelines of Korea Food and Drug Administration (KFDA). Forty healthy male Korean volunteers were enrolled in the study and thirty six volunteers completed the study according to the protocol. Thirty six volunteers received each medicine at the lacidipine dose of 4 mg in a $2{\times}2$ crossover study. There was one week wash-out period between the doses. Plasma concentrations of lacidipine were monitored by a high performance liquid chromatography - tandem mass spectrometry (LC-MS/MS) for over a period of 24 hours after drug administration. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 24 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for LANIDIEM$^{(R)}$/Vaxar$^{(R)}$ were log 0.8102~log 1.0417 and log 0.8493~log 1.1439, respectively. These values were within the acceptable bioequivalence intervals of log 0.80~log 1.25. Thus, our study demonstrated the bioequivalence of LANIDIEM$^{(R)}$ tablet 4 mg and Vaxar$^{(R)}$ tablet 4 mg with respect to the rate and extent of absorption.