• 한국안전학회지
• /
• 제29권6호
• /
• pp.166-171
• /
• 2014

Nuclear power plants are equipped with the reactor trip system (RTS) and the engineered safety features actuation system (ESFAS) to improve safety on the normal operation. In the event of the design basis accident (DBA), a various of post accident monitor(PAM)systems support to provide important details (e.g. Containment pressure, temperature and pressure of reactor cooling system and core exit temperature) to determine action of main control room (MCR). Operator should be immediately activated for the accident mitigation with the information. Especially, core exit temperature is a critical parameter because the operating mode converts from normal mode to emergency mode when the temperature of core exit reaches $649^{\circ}C$. In this study, uncertainty which was caused by exterior environment, characteristic of thermocouple/connector and accuracy of calibrator/indicator was evaluated in accordance with ANSI-ISA 67.04. The square root of the sum of square (SRSS) methodology for combining uncertainty terms that are random and independent was used in the synthesis. Every uncertainty that may exist in the hardware which is used to measure the core exit temperature was conservatively applied and the associative relation between the elements of uncertainty was considered simultaneously. As a result of uncertainty evaluation, the channel statistical allowance (CSA) of single channel of core exit temperature was +1.042%Span. The range of uncertainty, -0.35%Span ($-4.05^{\circ}C$) ~ +2.08%Span($24.25^{\circ}C$), was obtained as the operating criteria of core exit temperature.

• Toxicological Research
• /
• 제17권2호
• /
• pp.123-129
• /
• 2001

This study were carried out to investigate cytotoxicity of paraquat and bentazon that is scattering to farm products were essensial for human diet and compensatory effects of 3-methylcholanthrene (3-MC) in vitro and in vivo. In vitro, The 5.0$\times$$10^4$ cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution of paraquat and bentazon (1, 25, 50, 100 pM respectively). After the NIH 3T3 fibroblast of all groups were cultured in same condition for 48 hours, Sulfohordamin B Protein (SRB) assay were performed to evaluate the cytotoxicity of cell organelles. Paraquat and bentazon $SRB_50$ were 1860.73 $\mu\textrm{M}$, 1913.38 $\mu$M respectively. In vivo, Sprague Dawley male rats divided into paraquat and bentazon only administered group and simultaneous application group of paraquat and bentazon and 3-MC. At 30 min. and 1, 3, 6, 12, 24, 48 and 96 hrs. interval after each treatment, the animals were sacrificed by decapitation and kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM, and PAS. Under the light microscope, atrophic change of renal corpuscles were frequently observed from 3 hrs after paraquat and bentazon treatment. The increase of the mesangium was apparent from 12 hrs later after paraquat and bentazon treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 48 hrs after paraquat and bentazon treatment, respectively. In contrast there were no evidences of the toxic effects on renal tissues at 48hrs in paraquat and bentazon plus 3-MC treated groups.

• 한국농공학회논문집
• /
• 제56권3호
• /
• pp.31-38
• /
• 2014

Four actual cultivations were prepared and a variety of soybean was cultivated. A H-flume, an automatic water level gauge and an automatic water sampler were installed at the outlet of each plot equipped for the measurement of flow rate and its water quality. The amount of rainfall of the study area in 2013 was measured as 975.6 mm which was much lower than the annual average rainfall of 1,271.8 mm, resulting in less occurrences in rainfall-runoff events. Rainfall-runoff events were occurred three times during the rainfall event of 4~5 July, 23 and 24 August. The characteristics of NPS pollution discharge of the plots and the reduction effect of the selected BMPs were analyzed during these events. The reduction effect of straw mat and soil amendments (Polyacrylamide (PAM) and Gypsum) on runoff ratio ranged between 38.2 and 92.9% (average 71.6%). The NPS pollution load reduced between 27.7 and 95.1% (average 70.0%) by the application of rice straw mat and soil conditioner when compared with that of control plot. Soybean yield (2,133.3 kg/ha) of the straw mat covered plots increased by 14.3% when compared with control (1,866.7 kg/ha). The effect of straw mat on the yield was not economically viable if the material and accompanying labor costs were considered. The data collected and analyzed on different soil textures and crops in this study are expected to be a fundamental reference for the expansion of the results to the application nationwide and the development of NPS pollution management policies.

• Journal of Ginseng Research
• /
• 제46권5호
• /
• pp.675-682
• /
• 2022

Background: Korean Red Ginseng (KRG) was reported to play an anti-inflammatory role, however, previous studies largely focused on the effects of KRG on priming step, the inflammation-preparing step, and the anti-inflammatory effect of KRG on triggering, the inflammation-activating step has been poorly understood. This study demonstrated anti-inflammatory role of KRG in caspase-11 non-canonical inflammasome activation in macrophages during triggering of inflammatory responses. Methods: Caspase-11 non-canonical inflammasome-activated J774A.1 macrophages were established by priming with Pam3CSK4 and triggering with lipopolysaccharide (LPS). Cell viability and pyroptosis were examined by MTT and lactate dehydrogenase (LDH) assays. Nitric oxide (NO)-inhibitory effect of KRG was assessed using a NO production assay. Expression and proteolytic cleavage of proteins were examined by Western blotting analysis. In vivo anti-inflammatory action of KRG was evaluated with the LPS-injected sepsis model in mice. Results: KRG reduced LPS-stimulated NO production in J774A.1 cells and suppressed pyroptosis and IL-1β secretion in caspase-11 non-canonical inflammasome-activated J774A.1 cells. Mechanistic studies demonstrated that KRG suppressed the direct interaction between LPS and caspase-11 and inhibited proteolytic processing of both caspase-11 and gasdermin D in caspase-11 non-canonical inflammasome-activated J774A.1 cells. Furthermore, KRG significantly ameliorated LPS-mediated lethal septic shock in mice. Conclusion: The results demonstrate a novel mechanism of KRG-mediated anti-inflammatory action that operates through targeting the caspase-11 non-canonical inflammasome at triggering step of macrophage-mediated inflammatory response.

• 품질경영학회지
• /
• 제51권1호
• /
• pp.97-117
• /
• 2023

Purpose: The purpose of this study is to examine the factors affecting the intention to continue using the metaverse platform service from the user's point of view. Methods: The study conducted a survey of experienced people using Zepeto, Ifland, Gather Town, Zep, Roblox, Fortnite, and Minecraft, which are virtual world types among metaverse types, and valid 415 data were analyzed by SPSS (ver 22.0) and R (ver 4.1.0) program packages. Results: As a result of empirical analysis, it was found that interactivity and social presence among the characteristics of metaverse affected expectation confirmation, and sensory affordance and functional affordance affected expectation confirmation as affordance factors. In addition, all four characteristics of metaverse, reliability, playfulness, interactivity, and social presence, were found to have the greatest affect on perceived usefulness, and it was analyzed that playfulness, social presence, and reliability were influenced in order. The affordance factor was also found to have a positive effect on physical affordance, sensory affordance, and functional affordance. This study was found that it had a positive effect on the relationship between expectation confirmation and satisfaction and the relationship between perceived usefulness and satisfaction. satisfaction was analyzed to lead to continuance intention. Conclusion: This study is meaningful in that it examines the perspective of users who can be called customers of metaverse platform services. Based on the results of this study, it is expected to have a significant effect on the development of metaverse platform services not only on platform operators that provide metaverse platform services but also on providers who plan events and education using metaverse platform services.

• Molecules and Cells
• /
• 제43권10호
• /
• pp.870-879
• /
• 2020

Dendrites require precise and timely delivery of protein substrates to distal areas to ensure the correct morphology and function of neurons. Many of these protein substrates are supplied in the form of ribonucleoprotein (RNP) complex consisting of RNA-binding proteins (RBPs) and mRNAs, which are subsequently translated in distal dendritic areas. It remains elusive, however, whether key RBPs supply mRNA according to local demands individually or in a coordinated manner. In this study, we investigated how Drosophila sensory neurons respond to the dysregulation of a disease-associated RBP, Ataxin-2 (ATX2), which leads to dendritic defects. We found that ATX2 plays a crucial role in spacing dendritic branches for the optimal dendritic receptive fields in Drosophila class IV dendritic arborization (C4da) neurons, where both expression level and subcellular location of ATX2 contribute significantly to this effect. We showed that translational upregulation through the expression of eukaryotic translation initiation factor 4E (eIF4E) further enhanced the ATX2-induced dendritic phenotypes. Additionally, we found that the expression level of another disease-associated RBP, fragile X mental retardation protein (FMRP), decreased in both cell bodies and dendrites when neurons were faced with aberrant upregulation of ATX2. Finally, we revealed that the PAM2 motif of ATX2, which mediates its interaction with poly(A)-binding protein (PABP), is potentially necessary for the decrease of FMRP in certain neuronal stress conditions. Collectively, our data suggest that dysregulation of RBPs triggers a compensatory regulation of other functionally-overlapping RBPs to minimize RBP dysregulation-associated aberrations that hinder neuronal homeostasis in dendrites.

• 한국식품위생안전성학회지
• /
• 제22권1호
• /
• pp.45-51
• /
• 2007

본 연구에서는 현대인들에게 간식 또는 식사대용으로도 많이 소비되고 있는 샌드위치와 그 제조사업장에 대한 병원성 세균검사를 통하여 한 제조사업장의 행주에서 L. monocytogenes가 분리되었으며 모든 사업장의 원료와 샌드위치 20종에서 11주의 S. aureus를 분리하였으며, 이들 분리된 균주에 대하여 각각 26종과 20종의 항생제 내성 검사를 실시하여 샌드위치와 제조환경에 대한 항생제 노출정도를 알아보았다. C 사업장의 행주에서 분리된 L. monocytogenes는 총 26종의 항생제에 대하여 38.5%의 항생제 내성율을 나타내었다. penicillin계열의 항생제인 Am, OX, P에 모두 내성을 나타내었으며, aminoglycoside계열의 항생제인 AM, Gm, K, S, N에 대해서는 모두 감수성을 나타내었다. 총 70종의 시료에서 11주의 S. aureus가 검출되었으며 모두 구운 고기, 햄, 야채 등의 원료와 샌드위치에서 분리되었다. penicillin계열의 P와 Am에 90.9%로 가장 높은 내성율을 보였으며 OX에도 36.4%의 내성율을 나타내어 MRSA가 4주 확인되었다. 그리고 인체용 의약품 혹은 가축의 성장촉진제로서 사료에 첨가되는 B에 대하여 모든 분리균이 중간내성으로 나타나 B에 노출되어 있음을 시사하였다. PAmB 또는 OX을 포함하는 분리균주는 KECXM에 대해서도 내성을 나타내어 6, 7약제 내성형 이었으며 야채와 샌드위치에서 분리된 S. aureus라는 공통점을 보였다.

• 말소리와 음성과학
• /
• 제13권4호
• /
• pp.23-34
• /
• 2021

이 연구는 이집트인 한국어 학습자를 대상으로 하여 이들이 한국어 모음을 어떻게 지각하고 범주화하며, 이들이 발음한 한국어 모음을 한국인들이 어떻게 지각하는지 밝히고, 이를 토대로 이집트인 학습자들의 한국어 모음 범주화가 그들의 한국어 모음 지각과 산출에 어떤 영향을 미치는지 밝히는 것을 목적으로 한다. 실험 1에서는 이집트인 학습자가 한국어 모음을 어떻게 지각하는지 알아보기 위해 이집트인 학습자 53명을 대상으로 하여 한국인이 발음한 한국어 자극 단어를 듣고 어느 단어를 들었는지 객관식으로 고르는 과제를 수행하게 하였고, 실험 2에서는 이집트인 학습자들이 발음한 한국어 모음을 한국인들이 어떻게 지각하는지 밝히기 위해 이집트인 학습자 9명이 산출한 자극 단어 117(13개×9명)개를 한국인들에게 들려주고, 어느 단어를 들었는지 객관식으로 고르게 한 다음 모음의 발음이 원어민 수준에 얼마나 근접하는지 5점 척도로 평가하도록 하였다. 실험 결과 이집트어에 존재하지 않는 "새로운" 한국어 모음은 별도의 범주를 쉽게 형성하여 잘 지각된 반면 산출이 잘 되는 새로운 모음도 있었고, 산출에서 어려움을 겪는 모음도 있었다. 반면에 이집트어 음소와 "비슷한" 한국어 음소는 비교적 잘 산출되지만 지각하는 데는 큰 어려움이 있다는 사실도 확인할 수 있었다. 이 연구 결과를 토대로 기존의 음성학습모델(speech learning model)과 지각동화모델(perceptual assimilation model)이 제2언어 학습자들의 제2언어 음성 지각을 잘 설명해 주지만 음성 산출을 설명하는 데 미흡함이 있어 이에 대한 보완이 필요함을 논의했다.

• Asia pacific journal of information systems
• /
• 제18권3호
• /
• pp.91-121
• /
• 2008

Electronic commerce, commonly known as e-commerce or eCommerce, has become a major business trend in these days. The amount of trade conducted electronically has grown extraordinarily by developing the Internet technology. Most electronic commerce has being conducted between businesses to customers; therefore, the researches with respect to e-commerce are to find customer's needs, behaviors through statistical methods. However, the statistical researches, mostly based on a questionnaire, are the static researches, They can tell us the dynamic relationships between initial purchasing and repurchasing. Therefore, this study proposes dynamic research model for analyzing the cause of initial purchasing and repurchasing. This paper is based on the System-Dynamic theory, using the powerful simulation model with some restriction, The restrictions are based on the theory TAM(Technology Acceptance Model), PAM, and TPB(Theory of Planned Behavior). This article investigates not only the customer's purchasing and repurchasing behavior by passing of time but also the interactive effects to one another. This research model has six scenarios and three steps for analyzing customer behaviors. The first step is the research of purchasing situations. The second step is the research of repurchasing situations. Finally, the third step is to study the relationship between initial purchasing and repurchasing. The purpose of six scenarios is to find the customer's purchasing patterns according to the environmental changes. We set six variables in these scenarios by (1) changing the number of products; (2) changing the number of contents in on-line shopping malls; (3) having multimedia files or not in the shopping mall web sites; (4) grading on-line communities; (5) changing the qualities of products; (6) changing the customer's degree of confidence on products. First three variables are applied to study customer's purchasing behavior, and the other variables are applied to repurchasing behavior study. Through the simulation study, this paper presents some inter-relational result about customer purchasing behaviors, For example, Active community actions are not the increasing factor of purchasing but the increasing factor of word of mouth effect, Additionally. The higher products' quality, the more word of mouth effects increase. The number of products and contents on the web sites have same influence on people's buying behaviors. All simulation methods in this paper is not only display the result of each scenario but also find how to affect each other. Hence, electronic commerce firm can make more realistic marketing strategy about consumer behavior through this dynamic simulation research. Moreover, dynamic analysis method can predict the results which help the decision of marketing strategy by using the time-line graph. Consequently, this dynamic simulation analysis could be a useful research model to make firm's competitive advantage. However, this simulation model needs more further study. With respect to reality, this simulation model has some limitations. There are some missing factors which affect customer's buying behaviors in this model. The first missing factor is the customer's degree of recognition of brands. The second factor is the degree of customer satisfaction. The third factor is the power of word of mouth in the specific region. Generally, word of mouth affects significantly on a region's culture, even people's buying behaviors. The last missing factor is the user interface environment in the internet or other on-line shopping tools. In order to get more realistic result, these factors might be essential matters to make better research in the future studies.

• 한국환경농학회지
• /
• 제20권3호
• /
• pp.155-161
• /
• 2001

Paraquat와 bentazone이 횐쥐의 간조직과 NIH 3T3 섬유모세포에 미치는 독성과 그 독성에 대한 3-MC의 보상효과를 조사하기 위하여 NIH 373 섬유모세포에 적응한 후 경시적으로 MTT분석을 이용하여 세포독성을 측정하고 Sprague Dawley계 웅성 횐쥐에 paraquat와 bentazone단독 및 paraquat 및 bentazone과 3-MC를 병용투여한 후 경시적으로 관찰한 결과 paraquat와 bentazone은 NIH 3T3 섬유모세포에 대하여 $IC_{50}$값이 각각 1668.97 ${\mu}M$, 1506.97 ${\mu}M$으로 Borenfreund의 독성평가기준에 의하면 저독성이었다. Paraquat와 bentazone 단독투여군의 H&E 염색에서 3시간째에는 문맥 주위 세포들이 변성을 일으키고 별모양 세포들이 증가하였으나 12시간째에는 간소엽 전체의 세포들이 변성을 일으켰으며 48시간째에는 더욱 심한 변성이 일어났다. 특히 bentazone 투여 후 48시간째에는 핵농축현상이 뚜렷하였다. Best carmine 염색에서 glycogen 과립을 함유하는 간세포들도 3시간째에는 문맥주위의 세포들이, 12시간째에는 간소엽 전체의 간세포들이, 48시간째에는 전체의 간세포들이 함유하는 glycogen 과립량이 현저히 증가하였다. 3-MC를 paraquat와 bentazone과 동시에 투여한 군에서 3시간째와 12시간째에는 단독투여군과 유사하였으나 48시간째에는 bentazone과 3-MC 동시투여군의 문맥주위의 간세포들이 재생되는 경향이었으며 paraquat와 3-MC를 동시에 투여한 군에서는 중심정맥 주위의 세포들만이 glycogen과립을 함유하고 있어 단독 투여군과 뚜렷한 차이를 관찰할 수 있었다. 이 결론에서 3-MC는 paraquat와 bentazone에 의한 간세포의 독성을 경감시킬 수 있는 물질임을 알 수 있었다.