• Animal cells and systems
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• 제16권5호
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• pp.400-407
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• 2012

Plant growth regulators are the chemicals that are found in plants and produced synthetically. In agricultural applications, plant hormones are used in minor quantities for fixing the problems. In our research, we studied the effects of 2,4 diclorophenoxyacetic acid (2,4D), which is an auxin used in agricultural applications. Auxins are the group that are used most popularly in plant growth regulators. In our study, different doses of 2,4 diclorophenoxyacetic acid are given to zebrafish, and ovarium tissues are observed histomorphologically. We generated one control and three experiment groups from the stock solution. The experiment was carried out in 20 liter capacity complete glass aquarium at $24{\pm}1^{\circ}C$ water temperature. After five days of application, fishes were dissected. Histomorphological changes of the ovarium were investigated under a light microscope. A decrease in the number of oocytes in zebrafish ovarium was observed when compared with the control group. Many deformed and underdeveloped oocytes were detected. An increase in the number of atretic oocytes was observed. It was deduced that acute doses of 2,4 diclorophenoxyacetic acid decelerates oogenesis in fishes.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.87-93
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• 2003

Brachiaria (Poaceae) is the most important forage genus for cattle production in Brazil. The genetic breeding of this genus is limited by the incompatibility among species, differences in ploidy level and the natural cloning of plants by apomixis (Valle and Miles 1992). However, plant regeneration via tissue culture methods and genetic engineering provide an opportunity to introduce new characteristics in plants of this genus. We have developed methods for the 'genetic modification of Brachiaria brizantha cv. Marandu via biolistic transformation. A higher number of shoots was obtained with 4 mg/L 2.4-diclorophenoxyacetic acid and 0.2 mg/L benzylaminopurine in calli induction medium and 0.1 mg/L naphtaleneacetic acid and 4.0 mg/L kinetin in shoot regeneration medium. A selection curve for mannose was determined to use phospho mannose isomerase (PMI) gene of Escherichia coli as a selection marker. Calli formation was inhibited from 5 g/L mannose, even in the presence of sucrose while calli that were formed in the presence of mannose failed to develop embryos showing that PMI gene can be used for selection of transformants of this grass. Different promoters were tested to evaluate the efficiency based on the detection of the GUS gene expression (Jefferson et al. 1987). The monocot promoters, act1-D and ubi-1, resulted in higher expression levels than dicot promoters, ubi-3 and act-2, or the CaMV35S and CVMV promoters.