• The Korean Journal of Physiology and Pharmacology
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• 제24권4호
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• pp.287-298
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• 2020

Ca²⁺ signaling of endothelial cells plays a critical role in controlling blood flow and pressure in small arteries and arterioles. As the impairment of endothelial function is closely associated with cardiovascular diseases (e.g., atherosclerosis, stroke, and hypertension), endothelial Ca²⁺ signaling mechanisms have received substantial attention. Increases in endothelial intracellular Ca²⁺ concentrations promote the synthesis and release of endothelial-derived hyperpolarizing factors (EDHFs, e.g., nitric oxide, prostacyclin, or K⁺ efflux) or directly result in endothelial-dependent hyperpolarization (EDH). These physiological alterations modulate vascular contractility and cause marked vasodilation in resistance arteries. Transient receptor potential (TRP) channels are nonselective cation channels that are present in the endothelium, vascular smooth muscle cells, or perivascular/sensory nerves. TRP channels are activated by diverse stimuli and are considered key biological apparatuses for the Ca²⁺ influx-dependent regulation of vasomotor reactivity in resistance arteries. Ca²⁺-permeable TRP channels, which are primarily found at spatially restricted microdomains in endothelial cells (e.g., myoendothelial projections), have a large unitary or binary conductance and contribute to EDHFs or EDH-induced vasodilation in concert with the activation of intermediate/small conductance Ca²⁺-sensitive K⁺ channels. It is likely that endothelial TRP channel dysfunction is related to the dysregulation of endothelial Ca²⁺ signaling and in turn gives rise to vascular-related diseases such as hypertension. Thus, investigations on the role of Ca²⁺ dynamics via TRP channels in endothelial cells are required to further comprehend how vascular tone or perfusion pressure are regulated in normal and pathophysiological conditions.

• 대한약침학회지
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• 제6권3호
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• pp.91-106
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• 2003

Objectives : The aim of this study was to examine the effects of electroacupuncture(EA) at the PC6(Naegwan) and the TE5 (Oegwan) on nounal humans using power spectral analysis. Methods : EEG power spectrum exhibit site-specific and state-related differences in specific frequency bands. In this study, power spectrum was used as a measure of complexity. 30 channel EEG study was carried out in 30 subjects(30 males ; age=23.7 years). Results : In ${\alpha}$(alpha) band, the power values at F7 channels(p<0.05) during the PC6-acupoint treatment were significantly were decreased. In ${\beta}$(beta) band, the power values at Fp1, Fz, TT1, T5, P3, P4, Po1, P02, O1, Oz, O2 channels(p<0.05) during the non-acupoint treatment and at Fp1, F4, F8 channels(p<0.05) during the TE5-acupoint treatment significantly were increased. In ${\theta}$(theta) band, the power values at Fp1 channels(p<0.05) during the non-acupoint treatment and at Oz channels(p<0.05) the TE5-acupoint treatment significantly were increased. but, the power values at F7 channels(p<0.05) during the non-acupoint treatment were significantly were decreased. In ${\delta}$(delta) band, the power values at TCP1, TCP2, CP1, T5 channels(p<0.05) during PC6-acupoint treatment were increased and the power values at F7, TT2 channels(p<0.05) during non-acupoint treatment were increased. but, the power values at the TE5-acupoint treatment significantly was decreased than the before-acupuncture treatment.

• 한국콘텐츠학회논문지
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• 제21권2호
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• pp.140-147
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• 2021

다양한 수면검사에서 수면단계를 정확히 판단하기 위해 뇌파를 측정한다. 일반적으로 측정은 센서 채널의 개수가 늘어날수록 정확도가 높아지지만, 뇌파는 측정할 때 피부에 전극을 부착하여 수면을 방해하는 요소로 작용한다. 일상생활에서 자가 수면케어를 할 때는 사용자의 불편함과 측정데이터의 정확도를 모두 고려한 최소한의 뇌파 채널 개수를 선택할 필요가 있다. 따라서 본 논문에서는 1개의 채널부터 4개의 채널에 대한 수면단계 분석 모델을 제작하여 1채널은 82.28%, 2채널은 85.77%, 3채널은 80.33%, 4채널은 68.87%의 정확도를 확인했다. 본 연구 결과는 측정 부위가 제한적이라는 한계가 있지만, 채널 개수에 따른 정확도를 비교하여 뇌파 기반 수면분석에서 채널 개수 선정에 대한 정보를 제공한다.

• Parasites, Hosts and Diseases
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• 제59권4호
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• pp.329-339
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• 2021

Ion channels are important targets of anthelmintic agents. In this study, we identified 3 types of ion channels in Ascaris suum tissue incorporated into planar lipid bilayers using an electrophysiological technique. The most frequent channel was a large-conductance cation channel (209 pS), which accounted for 64.5% of channels incorporated (n=60). Its open-state probability (P_o) was ~0.3 in the voltage range of -60~+60 mV. A substate was observed at 55% of the main-state. The permeability ratio of Cl^- to K⁺ (P_Cl/P_K) was ~0.5 and P_Na/P_K was 0.81 in both states. Another type of cation channel was recorded in 7.5% of channels incorporated (n=7) and discriminated from the large-conductance cation channel by its smaller conductance (55.3 pS). Its Po was low at all voltages tested (~0.1). The third type was an anion channel recorded in 27.9% of channels incorporated (n=26). Its conductance was 39.0 pS and P_Cl/P_K was 8.6±0.8. P_o was ~1.0 at all tested potentials. In summary, we identified 2 types of cation and 1 type of anion channels in Ascaris suum. Gating of these channels did not much vary with voltage and their ionic selectivity is rather low. Their molecular nature, functions, and potentials as anthelmintic drug targets remain to be studied further.

• The Korean Journal of Physiology and Pharmacology
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• 제27권4호
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• pp.399-406
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• 2023

Voltage-dependent K⁺ (Kv) channels are widely expressed on vascular smooth muscle cells and regulate vascular tone. Here, we explored the inhibitory effect of encainide, a class Ic anti-arrhythmic agent, on Kv channels of vascular smooth muscle from rabbit coronary arteries. Encainide inhibited Kv channels in a concentration-dependent manner with an IC₅₀ value of 8.91 ± 1.75 μM and Hill coefficient of 0.72 ± 0.06. The application of encainide shifted the activation curve toward a more positive potential without modifying the inactivation curve, suggesting that encainide inhibited Kv channels by altering the gating property of channel activation. The inhibition by encainide was not significantly affected by train pulses (1 and 2 Hz), indicating that the inhibition is not use (state)-dependent. The inhibitory effect of encainide was reduced by pretreatment with the Kv1.5 subtype inhibitor. However, pretreatment with the Kv2.1 subtype inhibitor did not alter the inhibitory effects of encainide on Kv currents. Based on these results, encainide inhibits vascular Kv channels in a concentration-dependent and use (state)-independent manner by altering the voltage sensor of the channels. Furthermore, Kv1.5 is the main Kv subtype involved in the effect of encainide.

• The Korean Journal of Physiology and Pharmacology
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• 제24권1호
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• pp.101-110
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• 2020

Transient receptor potential canonical 4 (TRPC4) channel is a nonselective calcium-permeable cation channels. In intestinal smooth muscle cells, TRPC4 currents contribute more than 80% to muscarinic cationic current (mIcat). With its inward-rectifying current-voltage relationship and high calcium permeability, TRPC4 channels permit calcium influx once the channel is opened by muscarinic receptor stimulation. Polyamines are known to inhibit nonselective cation channels that mediate the generation of mIcat. Moreover, it is reported that TRPC4 channels are blocked by the intracellular spermine through electrostatic interaction with glutamate residues (E728, E729). Here, we investigated the correlation between the magnitude of channel inactivation by spermine and the magnitude of channel conductance. We also found additional spermine binding sites in TRPC4. We evaluated channel activity with electrophysiological recordings and revalidated structural significance based on Cryo-EM structure, which was resolved recently. We found that there is no correlation between magnitude of inhibitory action of spermine and magnitude of maximum current of the channel. In intracellular region, TRPC4 attracts spermine at channel periphery by reducing access resistance, and acidic residues contribute to blocking action of intracellular spermine; channel periphery, E649; cytosolic space, D629, D649, and E687.

• International Journal of Internet, Broadcasting and Communication
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• 제12권4호
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• pp.42-47
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• 2020

YouTube, the online video streaming platform, has become popular and influential around the globe. Due to the development of science and technology, people without expertise in filming can now easily produce their videos with unique content. Many people are more eager to become a popular YouTube creator because they can earn money by placing commercials or Products in Placement (PPL) in their video clips. However, it is yet unknown what genres of YouTube videos are popular. YouTube creators have their channels where they upload videos of a certain type of genre. This study investigates video genres of the top 250 YouTube channels in English-speaking countries (United States, Canada, United Kingdom, and Australia) using Social Blade, which is a research website. The ranking is set based on the number of times people watched a video ("Video Views"). We handsomely analyze popular genres of the channels and also the YouTube ecosystem, and it will be meaningful for today's new media era.

• Journal of electromagnetic engineering and science
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• 제4권4호
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• pp.162-167
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• 2004

In this paper, optimal position of optical phase conjugator(OPC) for best compensating distorted WDM channels due to both chromatic dispersion and self phase modulation(SPM) is numerically investigated, and the compensation characteristics of overall WDM channels at this position is investigated, comparing with that in case of OPC placed at mid-way of total transmission length. It is confirmed that the compensation extents in WDM system with OPC is more improved by the shifting OPC position from the mid-way of total transmission length. And, we confirmed that the optimal position of OPC must be selected to the position decreasing not only eye opening penalty(EOP) of overall WDM channels but also EOP deviation between WDM channels, and this OPC position should be altered as various system parameters such as modulation format, and fiber dispersion, etc. Using proposed configuration, it is possible to remove all in-line dispersion compensator, reducing span losses and system costs.

• Journal of electromagnetic engineering and science
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• 제4권4호
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• pp.190-196
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• 2004

An alternative solution to the problem of obtaining acceptable performances on a fading channel is the diversity technique, which is widely used to combat the fading effects of time-variant channels. The symbol error probability of M-ary DPSK(MDPSK), PSK(MPSK) and QAM(MQAM) systems using 2 branches from the branch with the largest signal-to-noise ratio(SNR) at the output of L-branch selection combining(SC), i.e., SC2 in frequency- nonselective slow Nakagami fading channels with an additive white Gaussian noise(AWGN) is derived theoretically. These performance evaluations allow designers to determine M-ary modulation methods for Nakagami fading channels.

• The Korean Journal of Pain
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• 제28권4호
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• pp.231-235
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• 2015

Damage to peripheral nerves or the spinal cord is often accompanied by neuropathic pain, which is a complex, chronic pain state. Increasing evidence indicates that alterations in the expression and activity of gap junction channels in the spinal cord are involved in the development of neuropathic pain. Thus, this review briefly summarizes evidence that regulation of the expression, coupling, and activity of spinal gap junction channels modulates pain signals in neuropathic pain states induced by peripheral nerve or spinal cord injury. We particularly focus on connexin 43 and pannexin 1 because their regulation vastly attenuates symptoms of neuropathic pain. We hope that the study of gap junction channels eventually leads to the development of a suitable treatment tool for patients with neuropathic pain.