Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Electrophysiological Properties of Ion Channels in Ascaris suum Tissue Incorporated into Planar Lipid Bilayers

  • Park, Kwon Moo (Department of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Sun-Don (Department of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jin Bong (Department of Physiology, College of Medicine, Chungnam National University) ;
  • Hong, Sung-Jong (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Ryu, Pan Dong (Department of Veterinary Pharmacology, College of Veterinary Medicine, Seoul National University)
  • Received : 2021.04.09
  • Accepted : 2021.07.19
  • Published : 2021.08.31
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Abstract

Ion channels are important targets of anthelmintic agents. In this study, we identified 3 types of ion channels in Ascaris suum tissue incorporated into planar lipid bilayers using an electrophysiological technique. The most frequent channel was a large-conductance cation channel (209 pS), which accounted for 64.5% of channels incorporated (n=60). Its open-state probability (Po) was ~0.3 in the voltage range of -60~+60 mV. A substate was observed at 55% of the main-state. The permeability ratio of Cl- to K+ (PCl/PK) was ~0.5 and PNa/PK was 0.81 in both states. Another type of cation channel was recorded in 7.5% of channels incorporated (n=7) and discriminated from the large-conductance cation channel by its smaller conductance (55.3 pS). Its Po was low at all voltages tested (~0.1). The third type was an anion channel recorded in 27.9% of channels incorporated (n=26). Its conductance was 39.0 pS and PCl/PK was 8.6±0.8. Po was ~1.0 at all tested potentials. In summary, we identified 2 types of cation and 1 type of anion channels in Ascaris suum. Gating of these channels did not much vary with voltage and their ionic selectivity is rather low. Their molecular nature, functions, and potentials as anthelmintic drug targets remain to be studied further.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant (2007-0054932 to PDR).

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