• 한국소음진동공학회논문집
• /
• 제14권3호
• /
• pp.253-263
• /
• 2004

The problem considered in this paper is about the collocation of sensor and actuator for the active control of sound and vibration. It is well-known that a point collocated sensor-actuator pair offers an unconditional stability with very high performance when it is used with a direct velocity feedback (DVFB) control, because the pair has strictly positive real (SPR) property. In order to utilize this SPR characteristics, a matched piezoelectric sensor and actuator pair is considered. but this pair suffers from the in-plane motion coupling problem with the out-of-plane motion due to the piezo sensor and actuator interaction. This coupling phnomenon limits the stability and performance of the matched pair with DVFBcontrol. As a new alternative, a point sensor and distributed piezoelectric actuator pair is also considered, which provides SPR property in all frequency range when the pair is implemented on a clamped-clapmed beam. The use of this sensor-actuator pair is highly expected for the applications to more practical active control of sound and vibration systems with the DVFB control strategy.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권4호
• /
• pp.227-232
• /
• 2003

A self-assembled monolayer of protein G was fabricated to develop an immunosensor based on surface plasmon resonance (SPR), thereby improving the performance of the antibodybased biosensor through immobilizing the antibody molecules (lgG). As such, 11-mercaptoundecanoic acid (11-MUA) was adsorbed on a gold (Au) support, while the non-reactive hydrophilic surface was changed through substituting the carboxylic acid group (-COOH) in the 11-MUA molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholide (EDAC). The formation of the self-assembled protein G layer on the Au substrate and binding of the antibody and antigen were investigated using SPR spectroscopy, while the surface topographies of the fabricated thin films were analyzed using atomic force microscopy (AFM). A fabricated monoclonal antibody (Mab) layer was applied for detecting E. coli O157:H7. As a result, a linear relationship was achieved between the pathogen concentration and the SPR angle shift, plus the detection limit was enhanced up to 10$^2$ CFU/mL.

• Journal of Microbiology and Biotechnology
• /
• 제12권5호
• /
• pp.780-786
• /
• 2002

An immunosensor based on surface plasmon resonance (SPR) with a self-assembled protein G layer was developed for the detection of Escherichia coli O157:H7. A self-assembled protein C layer on a gold (Au) surface was fabricated by adsorbing the mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol at various molar ratios and by activating chemical binding between free amine (-$NH_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) in series. The formation of a self-assembled protein G layer on an Au substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of the self-assembled protein G layer on the Au substrate, monoclonal antibody (Mab) against E. coli O157:H7 which was immobilized on protein G, and bound E. coli O157:H7 extracts on Immobilized Mab against E. coii O157:H7 were performed by atomic force microscopy (AFM). The detection limit of the SPR-based immunosensor for E. coli O157:H7 was found to be about $10^4$ cells/ml.

• 한국안전학회지
• /
• 제31권3호
• /
• pp.1-7
• /
• 2016

In this study, static and fatigue tests on the self-piercing riveted (SPR) joint were conducted using cross-shaped specimens with aluminum alloy (Al-5052) sheets. Mixed mode loading was achieved by changing the loading angles of 0, 45, and 90 degrees using a special fixture to evaluate the static and fatigue strengths of the SPR joints under mixed mode loading conditions. Simulations of the specimens at three loading angles were carried out using the finite element code ABAQUS. The fatigue specimens failed in an interfacial mode where a crack initiated at the upper sheet and propagated along the longitudinal direction and finally fractured Maximum principal stress, von-Mises effective stress failed to correlate the fatigue lifetimes at three loading angles. However, the equivalent stress intensity factor was found to be appropriate to correlate the fatigue lifetimes at three loading angles.

• 한국소음진동공학회논문집
• /
• 제17권3호
• /
• pp.264-273
• /
• 2007

A smart panel with structural sensors and actuators for minimizing noise radiation or transmission is described in the paper with the concept of active structural acoustical control. The sensors and actuators are both quadratically shaped piezoelectric polyvinylidene fluoride(PVDF) Polymer films to implement a volume velocity sensor and uniform force actuator respectively. They are collocated on either side of the panel to take advantage of direct velocity feedback(DVFB) strategy, which can guarantee a robust stability and high performance as long as the sensor-actuator response is strictly positive real(SPR). However, the measured sensor-actuator response of the panel showed unexpected result with non-SPR property. In the paper, the reason of the non-SPR property is investigated by theoretical analysis, computer simulation and experimental verification. The investigation reveals that the arrangement of collocated piezoelectric PVDF sensor and actuator pair on a panel is not relevant to get a high feedback gain and good performance with DVFB strategy.

• 정보처리학회지
• /
• 제2권3호
• /
• pp.81-89
• /
• 1995

• 보건교육건강증진학회지
• /
• 제26권3호
• /
• pp.97-110
• /
• 2009

Objectives: This study investigated Korean public's beliefs on helpfulness of various resources and preferred ways of addressing symptoms of mental health problems. Methods: A household survey was conducted on a stratified sample of adults aged between 18 and 74 (N=1,584). The participants were asked to provide their opinion on helpfulness of 39 resources for problems presented in the vignette, either depression(DEP) or schizophrenia(SPR). Descriptive statistics and factor analysis were conducted to summarize their opinion and to identify the underlying structure of beliefs about helpfulness of various resources. Results: Counselors/psychologists/social workers, family, psychiatrists, other therapists, and religious leaders were believed to be the most helpful. Methods that require one's initiatives marked percentages much higher than those of 'waiting to get better' or 'dealing with it alone'. The percentages for medications were lower than other methods in general, but higher than those of a western sample especially on antidepressants and anti-psychotics. Psycho/social approach was favored more for SPR than for DEP, and popular/cultural approach was favored much more for DEP than for SPR; physical/medical approach was strongly unfavored against DEP. Conclusion: Strategies need to be developed for utilizing various helpful resources to diversify modality of mental health services and facilitate referrals and gatekeeping. The differences in beliefs on helpfulness of various methods between DEP and SPR point to the need for problem-specific strategies.

• 한국미생물·생명공학회지
• /
• 제49권4호
• /
• pp.510-518
• /
• 2021

Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae. N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)-functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 10³ to 10⁹ CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.

• Journal of Microbiology and Biotechnology
• /
• 제8권4호
• /
• pp.333-340
• /
• 1998

Streptomyces griseus trypsin (SGT) is an extracellular proteinase produced by S. griseus. The sprT gene, which encodes premature SGT protein, was cloned into the plasmid pWHM3, a Streptomyces-E. coli shuttle vector. When the recombinant plasmid was introduced into Streptomyces lividans TK24, two proteins with molecular weights of 28 kDa and 42 kDa were detected. The 28-kDa protein was a SGT protein while the larger 42-kDa protein is thought to have been a premature form of the SGT protein. The SGT protein was purified to homogeneity via ammonium sulfate fractionation and many column chromatographies, including CM -sepharose chromatography, Mono-S chromatography, and Superose-12 chromatography, from the culture broth of S. lividans TK24 harboring the sprT gene. The N-terminal amino acid sequence, isoelectric points, and stabilities at various conditions of the SGT proteins purified from the Pronase and transformant were almost identical. The amount of the expressed SGT in S. lividans TK 24 was determined to be 5 times more than that of S. griseus based on the enzymatic activity against artificial substrate.

• 한국지반공학회지:지반
• /
• 제3권1호
• /
• pp.53-64
• /
• 1987

도로포장의 구조평가 및 유지관리를 위하여 매우 효율적인 장비로 알려진 Dynaflect의 이용이 날 로 증가하고 있어, 이에 대한 연구가 절실하다. 따라서 본 연구에서는 도로포장에 사용되는 재료의 탄성계수에 따른 해석모질을 설정하고, 이를 다층토 이론에 근거한 콤퓨터 프로그램을 통하여 Dyn select의 처짐곡선을 산출함으로써 표층 두께와 탄성계수에 따른 분산도의 특성을 연구하였다.