• Genomics & Informatics
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• v.15 no.3
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• pp.98-107
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• 2017

MicroRNAs (miRNAs) act as regulators of gene expression by binding to the 3' untranslated region (UTR) of target genes. They perform important biological functions in the various species. Among many miRNAs, miR-21-3p is known to serve vital functions in development and apoptosis in olive flounder. Using genomic and bioinformatic tools, evolutionary conservation of miR-21-3p was examined in various species, and expression pattern was analyzed in olive flounder. Conserved sequences (5'-CAGUCG-3') in numerous species were detected through the stem-loop structure of miR-21-3p. Thus, we analyzed target genes of miR-21-3p. Among them, 3' UTR region of PPIL2 gene indicated the highest binding affinity with miR-21-3p based on the minimum free energy value. The PPIL2 gene showed high expression levels in testis tissue of the olive flounder, whereas miR-21-3p showed rather ubiquitous expression patterns except in testis tissue, indicating that miR-21-3p seems to control the PPIL2 gene expression in a complementary repression manner in various tissues of olive flounder. Taken together, this current study contributes to infer the target gene candidates for the miR-21-3p using bioinformatics tools. Furthermore, our data offers important information on the relationship between miR-21-3p and target gene for further functional study.

• Journal of Life Science
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• v.24 no.7
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• pp.798-803
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• 2014

Cathepsin D (CtsD), an aspartyl peptidase, is involved in apoptosis, resulting in the release of cytochrome C from mitochondria in cells. Here, we investigated microRNA regulation of CtsD expression in 3T3-L1 cells First, we observed the expression of CtsD in cells in response to doxorubicin (Dox). As expected, the level of CtsD mRNA was increased in 3T3-L1 cells exposed to Dox in a dose-dependent manner. Cellular viability of ectopically expressed CtsD cells was also decreased. Next, we used the miRanda program to search for particular microRNA targeting CtsD. MiR-145 was selected as a putative controller for CtsD because miR-145 had a high mirSVR score. In a reporter assay, the luciferase activity of cells containing the CtsD 3'-UTR region was decreased in cells transfected with miR-145 mimic compared to that of a control. The level of CtsD expression was down-regulated in preadipocytes ectopically expressing miR-145 and up-regulated by an miR-145 inhibitor. Cells also suppressed miR-145 expression when exposed to Dox. The miR-145 inhibitor reduced the cellular viability of 3T3-L1 cells. Taken together, these data suggest that miR-145 regulates CtsD-mediated cell death in adipocytes. These findings may have valuable implications concerning the molecular mechanism of CtsD-mediated cell death in obesity, suggesting that CtaD could be a useful therapeutic tool for the prevention and treatment of obesity by regulating fat cell numbers.

• Journal of Power System Engineering
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• v.12 no.4
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• pp.26-31
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• 2008

The evaporation heat transfer coefficient and pressure drop of R-22 and R-407C in a small diameter copper tube were investigated experimentally. The main components of the refrigerant loop are a receiver, a compressor, a mass flow mete, a condense and a double pipe type evaporate (test section). The test section consists of a smooth copper tube of 4.3 mm inner diameter. The refrigerant mass fluxes were varied from 100 to $300[kg/m^{2}s]$ and the saturation temperature of evaporator were $5[^{\circ}C]$. The evaporation heat transfer coefficients of R-22 and R-407C increase with the Increase in mass flux and vapor quality. The evaporation heat transfer coefficient of R-22 is about $7.3\sim47.1%$ higher than that of R-407C. The evaporation pressure drop of R-22 and R-407C increase with the increase of mass flux. The pressure drop of R-22 is about $8\sim20%$ higher than that of R-407C.

• Journal of the Korean Solar Energy Society
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• v.33 no.2
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• pp.1-10
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• 2013

Simulation study of a solar hybrid heat pump using R744 and R22 for residential applications was carried out according to heat pump operating temperature, outdoor temperature and solar radiation. As a result, when the heat pump operating temperature increases from $40^{\circ}C$ to $48^{\circ}C$, the COP of a R744 and R22 heat pump system decrease from 2.15 to 1.7 and from 3.09 to 2.69, respectively. Besides, as the outdoor temperature rises from $3^{\circ}C$ to $11^{\circ}C$, the COP of R744 and R22 heat pump system increase from 1.73 to 2.12 and from 2.73 to 3.02. When the solar radiation increases from 10 to 20 $MJ/m^2$, the collector operating time and collector efficiency of R744 heat pump increase 10.3 times and 50.7%, respectively. The performance of R744 solar hybird heat pump is more sensitive to operation condition compared to that of R22. Besides, the solar heating system is more effective to the R744 heat pump system.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8595-8601
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• 2014

Background: miR-200a expression is frequently altered in numerous cancers. The aim of the present study was to determine the role of microRNA-200a in advanced ovarian carcinomas. Materials and Methods: We measured miR-200a expression in 72 matched normal ovarian tissues and advanced ovarian carcinomas, and also two ovarian carcinoma cell lines (SKOV3 and SKOV3.ip1 - the latter being more invasive and metastatic than the parental SKOV3) by stem-loop real-time RT-PCR based on TaqMan microRNA assay using U6 as a reference. Levels of miR-200a expression were compared by disease stage, tumor grade, histology, and lymph node involvement. To evaluate the role of microRNA-200a, cell proliferation and invasion of SKOV-3 and SKOV-3.ip1 were analyzed with miR-200a inhibitor/mimic transfected cells. Results: Of 72 paired samples, 65 cancer tissues overexpressed microRNA-200a greater than two fold in comparison with matched normal epithelium. Specifically, patients with lymph node metastasis showed significant elevation. The level correlated with clinicopathological features, including high tumor grade, late disease stage, most notably with lymph node metastasis, but not with tumor histology. In addition, SKOV-3.ip1 cells also overexpressed miR-200a compared with SKOV-3, and miR-200a inhibitor transfected SKOV-3.ip1 cells showed significant reduction in cellular proliferation and invasion, while a miR-200a mimic stimulated the opposite behavior. Conclusions: We provide definitive evidence that miR-200a is up-regulated in a significant proportion of advanced ovarian carcinomas, and that elevated miR-200a expression facilitates tumor progression. Our findings support the notion that miR-200a is an onco-microRNA for ovarian cancer, and elevation is a useful potential diagnostic indicator. This study also provides a solid basis for further functional analysis of miR-200a in advanced ovarian cancer.

• Genomics & Informatics
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• v.19 no.2
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• pp.17.1-17.13
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• 2021

Breast cancer is one of the leading causes of cancer in women all over the world and accounts for ~25% of newly observed cancers in women. Epigenetic modifications influence differential expression of genes through non-coding RNA and play a crucial role in cancer regulation. In the present study, epigenetic regulation of gene expression by in-silico analysis of histone modifications using chromatin immunoprecipitation sequencing (ChIP-Seq) has been carried out. Histone modification data of H3K4me3 from one normal-like and four breast cancer cell lines were used to predict miRNA expression at the promoter level. Predicted miRNA promoters (based on ChIP-Seq) were used as a probe to identify gene targets. Five triple-negative breast cancer (TNBC)-specific miRNAs (miR153-1, miR4767, miR4487, miR6720, and miR-LET7I) were identified and corresponding 13 gene targets were predicted. Eight miRNA promoter peaks were predicted to be differentially expressed in at least three breast cancer cell lines (miR4512, miR6791, miR330, miR3180-3, miR6080, miR5787, miR6733, and miR3613). A total of 44 gene targets were identified based on the 3'-untranslated regions of downregulated mRNA genes that contain putative binding targets to these eight miRNAs. These include 17 and 15 genes in luminal-A type and TNBC respectively, that have been reported to be associated with breast cancer regulation. Of the remaining 12 genes, seven (A4GALT, C2ORF74, HRCT1, ZC4H2, ZNF512, ZNF655, and ZNF608) show similar relative expression profiles in large patient samples and other breast cancer cell lines thereby giving insight into predicted role of H3K4me3 mediated gene regulation via the miRNA-mRNA axis.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.3
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• pp.277-287
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• 2023

Actin dynamics play an essential role in myogenesis through multiple mechanisms, such as mechanotransduction, cell proliferation, and myogenic differentiation. Twinfilin-1 (TWF1), an actin-depolymerizing protein, is known to be required for the myogenic differentiation of progenitor cells. However, the mechanisms by which they epigenetically regulate TWF1 by microRNAs under muscle wasting conditions related to obesity are almost unknown. Here, we investigated the role of miR-103-3p in TWF1 expression, actin filament modulation, proliferation, and myogenic differentiation of progenitor cells. Palmitic acid, the most abundant saturated fatty acid (SFA) in the diet, reduced TWF1 expression and impeded myogenic differentiation of C2C12 myoblasts, while elevating miR-103-3p levels in myoblasts. Interestingly, miR-103-3p inhibited TWF1 expression by directly targeting its 3'UTR. Furthermore, ectopic expression of miR-103-3p reduced the expression of myogenic factors, i.e., MyoD and MyoG, and subsequently impaired myoblast differentiation. We demonstrated that miR-103-3p induction increased filamentous actin (F-actin) and facilitated the nuclear translocation of Yes-associated protein 1 (YAP1), thereby stimulating cell cycle progression and cell proliferation. Hence, this study suggests that epigenetic suppression of TWF1 by SFA-inducible miR-103-3p impairs myogenesis by enhancing the cell proliferation triggered by F-actin/YAP1.

• Honam Mathematical Journal
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• v.34 no.1
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• pp.45-54
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• 2012

In this paper, we prove stabilities of multiplicative functional equations in three variables such as $r(\frac{x+y+z}{3})-r(x+y+z)$=$\frac{2r(\frac{x+y}{2})r(\frac{y+z}{2})r(\frac{z+x}{2})}{r(\frac{x+y}{2})r(\frac{y+z}{2})+r(\frac{y+z}{2})r(\frac{z+x}{2})+r(\frac{z+x}{2})r(\frac{x+y}{2})}$ and $r(\frac{x+y+z}{3})+r(x+y+z)$=$\frac{4r(\frac{x+y}{2})r(\frac{y+z}{2})r(\frac{z+x}{2})}{r(\frac{x+y}{2})r(\frac{y+z}{2})+r(\frac{y+z}{2})r(\frac{z+x}{2})+r(\frac{z+x}{2})r(\frac{x+y}{2})}$.

• Journal of the Korea Society of Computer and Information
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• v.10 no.4 s.36
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• pp.65-75
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• 2005

Moving-objects databases should efficiently support database queries that refer to the trajectories and positions of continuously moving objects. To improve the performance of these queries. an efficient indexing scheme for continuously moving objects is required. To my knowledge, range queries on current positions cannot be handled by the 3D R-tree and the TB-tree. In order to handle range queries on current and past positions. I modified the original 3D R-tree to keep the now tags. Most of spatio-temporal index structures suffer from the fact that they cannot efficiently process range queries past positions of moving objects. To address this issue. we propose an access method, called the Tagged Adaptive 3DR-tree (or just TA3DR-tree), which is based on the original 3D R-tree method. The results of our extensive experiments show that the Tagged Adaptive 3DR-tree outperforms the original 3D R-tree and the TB-tree typically by a big margin.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1216-1226
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• 2015

GlxR is considered as a global transcriptional regulator controlling a large number of genes having broad physiological aspects in Corynebacterium glutamicum. However, the expression profile revealing the transcriptional control of glxR has not yet been studied in detail. DNA affinity chromatography experiments revealed the binding of transcriptional regulators SucR, RamB, GlxR, and a GntR-type protein (hereafter denoted as GntR3) to the upstream region of glxR. The binding of different regulators to the glxR promoter was confirmed by EMSA experiments. The expression of glxR was analyzed in detail under various carbon sources in the wild-type and different mutant strains. The sucR and gntR3 deletion mutants showed decreased glxR promoter activities, when compared with the wild type, irrespective of the carbon sources. The promoter activity of glxR was derepressed in the ramB deletion mutant under all the tested carbon sources. These results indicate that SucR and GntR3 are acting as activators of GlxR, while RamB plays a repressor. As expected, the expression of glxR in the cyaB and glxR deletion mutants was derepressed under different media conditions, indicating that GlxR is autoregulated.