• Title/Summary/Keyword: 3D-MAC

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Enhanced TDMA based MAC Protocol for Adaptive Data Control in Wireless Sensor Networks

  • Alvi, Ahmad Naseem;Bouk, Safdar Hussain;Ahmed, Syed Hassan;Yaqub, Muhammad Azfar;Javaid, Nadeem;Kim, Dongkyun
    • Journal of Communications and Networks
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    • v.17 no.3
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    • pp.247-255
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    • 2015
  • In this paper, we propose an adaptive time division multiple access based medium access control (MAC) protocol, called bitmap-assisted shortest job first based MAC (BS-MAC), for hierarchical wireless sensor networks (WSNs). The main contribution of BS-MAC is that: (a) It uses small size time slots. (b) The number of those time slots is more than the number of member nodes. (c) Shortest job first (SJF) algorithm to schedule time slots. (d) Short node address (1 byte) to identify members nodes. First two contributions of BS-MAC handle adaptive traffic loads of all members in an efficient manner. The SJF algorithm reduces node's job completion time and to minimize the average packet delay of nodes. The short node address reduces the control overhead and makes the proposed scheme an energy efficient. The simulation results verify that the proposed BS-MAC transmits more data with less delay and energy consumption compared to the existing MAC protocols.

D-Methionine and 2-hydroxy-4-methylthiobutanoic acid i alter beta-casein, proteins and metabolites linked in milk protein synthesis in bovine mammary epithelial cells

  • Seung-Woo, Jeon;Jay Ronel V., Conejos;Jae-Sung, Lee;Sang-Hoon, Keum;Hong-Gu, Lee
    • Journal of Animal Science and Technology
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    • v.64 no.3
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    • pp.481-499
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    • 2022
  • This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

Relay Cooperative Transmission Scheme for Distributed MAC Protocol-Based Logistic Applications (분산적인 매체접근제어(MAC) 프로토콜 기반 물류 시스템을 위한 릴레이 협력통신 방안)

  • Joo, Yang-Ick;Hur, Kyeong
    • Journal of Korea Multimedia Society
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    • v.14 no.3
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    • pp.423-432
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    • 2011
  • In a warehouse, because there exist frequent changes of stock status and the quality of some contents are influenced by the environment such as temperature, fast and accurate management of the warehouse's environment is very important for Warehouse Management Systems (WMS). However, due to the absence of a unified standard for the communication protocol between RFID nodes, the connection between RFID nodes can be broken in case of movement of a RFID reader to a region with a different protocol. Moreover, centralized MAC schemes for RFID communications in previous studies have severe problems. For an example, if a cluster header disappears from the cluster due to the cluster header's movement or bad channel conditions, the RFID member nodes of the cluster waste lots of time and energy to re-elect a new cluster header. Therefore, in this paper, we propose a WiMedia Distributed MAC (D-MAC) scheme for RFID communications and its cooperative relay transmission scheme for WMS applications Simulation results show performance improvement at the RFID node by using the proposed cooperative relay transmission scheme.

Study on the Correlation between Sasang Constitution and the Data of Pulse Diagnosis Device - A Systematic Review in Korean Articles - (사상체질과 맥진기 검사 결과와의 상관성에 대한 연구 - 국내 논문에 대한 체계적 고찰 -)

  • Lee, Hye Yoon;Jung, A Ram;Son, Han Beom;Hwang, Man Suk;Lee, Jeong Won;Kim, Gyeong Cheol;Yun, Young Ju
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.5
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    • pp.621-629
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    • 2012
  • This study aimed to examine the possibility of data from pulse diagnosis device to be used for diagnosis of Sasang constitution. Systematic searches of 5 major Korean medical database were conducted for articles published up to May 2012. Searching key word was "Sasang" or "Constitution". Studies dealt with correlation between Sasang constitution and the pulse wave data from pulse diagnosis machine were included. Totally 2886 studies are searched and 3 studies are added from references of evaluated articles. Among them, 12 studies were met our inclusion criteria (2 Xishu Mac, 2 Self-made pulse diagnosis machine, 8 3D-Mac). Three of the eight 3D-Mac studies intended to develop a formula of constitutional differential diagnosis, 2 studies compared variables by Sasang constitution in healthy group and 3 studies compared variables by Sasang constitution in healthy group and special disease group. They all reported some significant variables, however results are not consistent between studies. The accuracy of the formula of constitutional differential diagnosis using 3D-Mac pulse diagnosis devices is 46.0% by now. Improvements in measuring pulse are required to achieve more accurate result and be used for diagnosis of Sasang constitution. Compensating B.M.I. among Sasang constitutional groups and separating constitutional pulse factors from acquired characteristics are also needed in further study.

Relay Transmission Protocol for Mobility Support in WiMedia Distributed MAC Systems (WiMedia Distributed MAC 통신 시스템에서 이동성 지원을 위한 릴레이 통신 프로토콜)

  • Hur, Kyeong
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.3
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    • pp.526-534
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    • 2014
  • In this paper, for the WiMedia Distributed Medium Access Control (D-MAC) protocol based on UWB. performance degradation due to the Distributed Reservation Protocol (DRP) conflict problem caused by devices' mobility is analyzed. And a DRP relay protocol and a DRP conflict resolution (CR) are proposed to overcome the performance degradation at DRP conflicts. In order to give the loser device at DRP conflicts a chance to maintain resources, the proposed DRP relay protocol executed at each device helps the loser device reserve an indirect link maintaining the required resources via a relay node. Simulation results considering the mobile environment have indicated that the DRP relay combined with the CR prevent the throughput decrease even though mobility of devices increases.

An Enhanced WLAN MAC Protocol for Directional Broadcast (지향성 브로드캐스트를 위한 무선 LAN MAC 프로토콜)

  • Cha, Woo-Suk;Cho, Gi-Hwan
    • Journal of KIISE:Information Networking
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    • v.33 no.1
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    • pp.16-27
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    • 2006
  • The wireless transmission medium inherently broadcasts a signal to all neighbor nodes in the transmission range. Existing asynchronous MAC protocols do not provide a concrete solution for reliable broadcast in link layer. This mainly comes from that an omni-directional broadcasting causes to reduce the network performance due to the explosive collisions and contentions. This paper proposes a reliable broadcast protocol in link taller based on directional antennas, named MDB(MAC protocol for Directional Broadcasting). This protocol makes use of DAST(Directional Antennas Statement Table) information and D-MACA(Directional Multiple Access and Collision Avoidance) scheme through 4-way handshake to resolve the many collision problem wit]1 omni-directional antenna. To analyze its performance, MDB protocol is compared with IEEE 802.11 DCF protocol [9] and the protocol 2 of reference [3], in terms of the success rate of broadcast and the collision rate. As a result of performance analysis through simulation, it was confirmed that the collision rate of the MDB protocol is lower than those of IEEE 802.11 and the protocol 2 of reference [3], and that the completion rate of broadcast of MDB protocol is higher than those of IEEE 802.11 and the protocol 2 of reference [3].

Correlations between Postpartum Edema and Pulse Energy Measured by 3D Blood Pressure Pulse Analyzer (3D Blood Pressure Pulse Analyzer로 측정한 맥파 에너지와 산후 부종과의 상관성 연구)

  • Lee, Ji-Yeon;Yun, Young-Jin
    • The Journal of Korean Obstetrics and Gynecology
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    • v.27 no.1
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    • pp.167-175
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    • 2014
  • Purpose: This study was performed to find correlations between Edema Index and Pulse Energy in early postpartum period. Methods: Fiftynine postpartum women were participated in this study which was conducted with Inbody 720 (Biospace Co. Ltd) and 3D Blood Pressure Pulse Analyzer (3D-MAC). Edema Index (Extra Cellular Water/Total Body Water) calculated after body composition analysis and 3D-MAC result (Pulse Energy) were analysed within a week after delivery. Results: The results were as follows 1. Edema Indexes of the upper, lower limbs, trunk and whole body were associated with each other respectively. 2. Edema Indexes and Right Kwan pulse energy had significant positive correlation. 3. Right Kwan pulse energy showed positive correlations with Left Kwan, Right Chon, Right Cheok. Conclusions: Viscera associated with Postpartum Edema are Spleen, Lung, Liver and Vital Gate. The most important thing is thought to be Spleen.

Electrospun Nanocomposite Fiber Mats of Zinc-Oxide Loaded Polyacrylonitrile

  • Nataraj, S.K.;Kim, B.H.;Yun, J.H.;Lee, D.H.;Aminabhavi, T.M.;Yang, K.S.
    • Carbon letters
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    • v.9 no.2
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    • pp.108-114
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    • 2008
  • We have demonstrated the feasibility of using electrospinning method to fabricate long and continuous composite nanofiber sheets of polyacrylonitrile (PAN) incorporated with zinc oxide (ZnO). Such PAN/ZnO composite nanofiber sheets represent an important step toward utilizing carbon nanofibers (CNFs) as materials to achieve remarkably enhanced physico-chemical properties. In an attempt to derive these advantages, we have used a variety of techniques such as field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and high resolution X-ray diffraction (HR-XRD) to obtain quantitative data on the materials. The CNFs produced are in the diameter range of 100 to 350 nm after carbonization at $1000^{\circ}C$. Electrical conductivity of the random CNFs was increased by increasing the concentration of ZnO. A dramatic improvement in porosity and specific surface area of the CNFs was a clear evidence of the novelty of the method used. This study indicated that the optimal ZnO concentration of 3 wt% is enough to produce CNFs having enhanced electrical and physico-chemical properties.

Immunochemical study on the Role of ${\beta}_2$ Integrin in the Activation of Monocytes Upon Direct Contact with T Lymphocytes (T 세포 접촉에 의한 단핵구 활성화에서 ${\beta}_2$ Integrin의 역할에 관한 면역화학적 연구)

  • Lee, Suck-Cho;Lee, Ho;Oh, Kwi-Ok;Kim, Hyung-Seop
    • Journal of Periodontal and Implant Science
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    • v.29 no.2
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    • pp.333-350
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    • 1999
  • The modulation of leukocyte cell surface adhesion molecules may influence the development of cellular events that determine the course of the inflammatory process. Direct interaction between activated T cells and monocytes resulted in a large production of $IL-1{\beta}$ by monocytes. In this reactions, adhesion molecules play an important part, yet the role of them in Tmonocytes interaction remain unclear. This study was undertaken in an effort to elucidate, 1) the influence of 1.25(OH)$_2D_3-induced$ differentiation on the monocyte responsiveness to direct contact with T lymphocytes, and 2) the role of adhesion molecules on the T-monocyte direct interaction. Initially, I observed that direct contact of monocyte cell line THP-1 with stimulated fixed T cell line HuT78 markedly induces IL-1${\beta}$ production by THP-1. $IL-1{\beta}$ production was higher when THP-1 had been previously exposed to 1.25(OH)$_2D_3$ as compared to control, with ${\alpha}$- 1.25(OH)$_2D_3$ dose-dependent and exposure time-dependent manner. It was shown that 1.25(OH)$_2D_3$ also increased the expression of ${\beta}_2$ integrin adhesion receptor Mac-1(CD11b/CD18) dose- and timedependently, but did not increase the expression of human leukocyte antigen- D(HLA-D) and intercellular adhesion molecule-1(ICAM-1). The $IL-1{\beta}$ producing activity of THP-1 cells correlated well with the ability to induce the Mac-1 expression on THP-1 surface. Monoclonal antibody raised against relevant cell surface glycoproteins on THP-1 were tested for their ability to block the response of THP-1 to T cells. Antibody to Mac-1 only partially blocked $IL-1{\beta}$ production by THP-1, whereas antibodies to ICAM-1 and HLA-D did not. These data indicate that regulation of Mac-1 expression on THP-1 cells can alter the responsiveness of these cells to contact by activated T cells, however other unknown structures on the THP-1 cells may be involved in this process also.

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Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

  • Liao, Xian-Dong;Zhou, Chang-Hai;Zhang, Jing;Shen, Jing-Lin;Wang, Ya-Jing;Jin, Yong-Cheng;Li, Sheng-Li
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.6
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    • pp.1012-1022
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    • 2020
  • Objective: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. Methods: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. Results: In MAC-T cells, ATRA increased the mRNA levels of αS1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA. Conclusion: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes.