• 제목/요약/키워드: 3D culture system

검색결과 409건 처리시간 0.024초

3D 스테레오스코픽(Stereoscopic)기술의 효율적 활용에 관한 연구 (Research on Efficient Usage of 3D Stereoscopic Technology)

  • 김지수
    • 한국콘텐츠학회논문지
    • /
    • 제10권2호
    • /
    • pp.138-145
    • /
    • 2010
  • 입체영상 기술은 차세대 입체 멀티미디어 정보통신 분야에서 공통적으로 요구되는 핵심 기반기술이라 할 수 있다. 현실과 가까운 자연스러운 영상을 표현하고자 하는 입체영상의 구현은 인간이 끊임없이 노력해온 부분으로, 초기의 시각 인식체계에서 시작해 양안시차를 이용한 입체사진을 거쳐 동영상 입체시대로 이행 되었다. 생활이 변화하고, 새로운 문화가 형성되고, IT산업 확장 등 핵심 트렌드의 바탕에는 가상을 실제처럼 구현하는 기술의 발전이 있으며, 그 중심에는 3D 입체영상 기술이 있다. 본 논문에서는 3D 입체영상 기술개발 동향 및 개발전략을 살펴보고, 지속적인 시장 확대를 위한 3D 입체영상 기술의 효율적 활용방안을 제시하고자 한다.

한국 첨단무기 신속개발 발전방안 (The Plans for Rapid Development of Advanced Weapons in Korea)

  • 박정환
    • 문화기술의 융합
    • /
    • 제9권5호
    • /
    • pp.27-33
    • /
    • 2023
  • 우크라이나와 러시아의 전쟁이 계속되고 있다. 우크라이나는 미국 등의 도움으로 인공지능 적용 첨단무기를 갖추어 러시아에 우세한 전투를 벌이고 있다. 이러한 추세에 한국군은 4차 산업혁명 국방기술에 대한 투자를 확대하고 스마트 군대를 구현하기 위하여 국방혁신 4.0을 발표하였다. 이를 효과적으로 달성하기 위해서는 무기 연구개발 제도를 살펴볼 필요가 있다. 이 논문은 기존 무기 연구개발 제도를 살펴보고 첨단무기를 적기에 신속개발 할 수 있는 발전 방안을 도출하였다. 그리고 최근 도입된 4차 산업혁명 기술 적용을 위한 신속연구개발에 대한 발전방안도 추가하여 제시하였으며 이를 통해 한국군이 신속하게 첨단무기를 도입하는데 도움이 되도록 하였다.

Lupus Heart Disease Modeling with Combination of Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Lupus Patient Serum

  • Narae Park;Yeri Alice Rim;Hyerin Jung;Yoojun Nam;Ji Hyeon Ju
    • International Journal of Stem Cells
    • /
    • 제15권3호
    • /
    • pp.233-246
    • /
    • 2022
  • Background and Objectives: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease mainly affecting young women of childbearing age. SLE affects the skin, joints, muscles, kidneys, lungs, and heart. Cardiovascular complications are common causes of death in patients with SLE. However, the complexity of the cardiovascular system and the rarity of SLE make it difficult to investigate these morbidities. Patient-derived induced pluripotent stem cells (iPSCs) serve as a novel tool for drug screening and pathophysiological studies in the absence of patient samples. Methods and Results: We differentiated CMs from HC- and SLE-iPSCs using 2D culture platforms. SLE-CMs showed decreased proliferation and increased levels of fibrosis and hypertrophy marker expression; however, HC-and SLE-monolayer CMs reacted differently to SLE serum treatment. HC-iPSCs were also differentiated into CMs using 3D spheroid culture and anti-Ro autoantibody was treated along with SLE serum. 3D-HC-CMs generated more mature CMs compared to the CMs generated using 2D culture. The treatment of anti-Ro autoantibody rapidly increased the gene expression of fibrosis, hypertrophy, and apoptosis markers, and altered the calcium signaling in the CMs. Conclusions: iPSC derived cardiomyocytes with patient-derived serum, and anti-Ro antibody treatment could serve in effective autoimmune disease modeling including SLE. We believe that the present study might briefly provide possibilities on the application of a combination of patient-derived materials and iPSCs in disease modeling of autoimmune diseases.

3D 바이오프린팅과 무지지체 조직공학 기술 기반 추간판 복합 조직 제작 (Fabrication of Tissue Engineered Intervertebral Disc Using Enable 3D bio-printing and Scaffod-Free technologies)

  • 김병국;박진호;박상혁
    • 대한의용생체공학회:의공학회지
    • /
    • 제39권1호
    • /
    • pp.22-29
    • /
    • 2018
  • Intervertebral disc(IVD) mainly consists of Annulus fibrosus(AF) and Nucleus pulposus(NP), playing a role of distributing a mechanical load on vertebral body. IVD tissue engineering has been developed the methods to achieve anatomic morphology and restoration of biological function. The goal of present study is to identify the possibilities for creating a substitute of IVD the morphology and biological functions are the same as undamaged complete IVD. To fabricate the AF and NP combine biphasic IVD tissue, AF tissue scaffolds have been printed by 3D bio-printing system with natural biomaterials and NP tissues have been prepared by scaffold-free culture system. We evaluated whether the combined structure of 3D printed AF scaffold and scaffold-free NP tissue construct could support the architecture and cell functions as IVD tissue. 3D printed AF scaffolds were printed with 60 degree angle stripe patterned lamella structure(the inner-diameter is 5mm, outer-diameter is 10 mm and height is 3 mm). In the cytotoxicity test, the 3D printed AF scaffold showed good cell compatibility. The results of histological and immunohistochemical staining also showed the newly synthesized collagens and glycosaminoglycans, which are specific makers of AF tissue. And scaffold-free NP tissue actively synthesized glycosaminoglycans and type 2 collagen, which are the major components of NP tissue. When we combined two engineered tissues to realize the IVD, combined biphasic tissues showed a good integration between the two tissues. In conclusion, this study describes the fabrication of Engineered biphasic IVD tissue by using enable techniques of tissue engineering. This fabricated biphasic tissue would be used as a model system for the study of the native IVD tissue. In the future, it may have the potential to replace the damaged IVD in the future.

Production of 3-Ketosteroid-delta-1-Dehydrogenase by a Two-stage Continuous Culture

  • Ryu, D.Y.;Lee, B.K.;Thoma, R.W.
    • 한국미생물·생명공학회지
    • /
    • 제2권1호
    • /
    • pp.29-35
    • /
    • 1974
  • We have studied the applicability of the principles and inherent advantages of the two-stage dontinuous uclture technique to an enzyme process for the purpose of improving and optimizing the productivity of 3-ketosteroid-delta-1-dehydrogenase. By using a two-stage continuous culture system, the growth st ageand enzyme produdtion stage are separated. In each stage an optimal set of toperaing conditions was determined, and this was tested for feasibility for the period of 10 days. During this period, at least 70% of the maximum enzyme productivity could be maintained. The important design parameters studied are: (1) optimal specific growth rate in the first stage which corresponds to the maximal cell productivity, (2) the optimal dilution rate in the second stage which in turn determines the size of second stage fermentor and the mean residence time of cells in the second stage, (3) cell concentration in both stages, add (4) the specific enzyme productivity and enzyme productivity of the second stage. In addition, by using two-stage continuous culture system we have been able to reduce or eliminate the effect of catabolite repression due to high medium concentration and the adverse effect of the solvent used to dissolve the inducer. We have found the balance between the opposing effects of induction and repression in the second stage judging from the observation that the enzyme productivity goes through a maximum.

  • PDF

양식해태의 유통에 관한 조사 연구 (A Study on Marketing of Cultured Laver Products)

  • 유충열
    • 수산경영론집
    • /
    • 제4권1_2호
    • /
    • pp.19-57
    • /
    • 1973
  • Laver io one of the most necessary and seasonal items in Korean food from oldtimes. Laver is lagely eaten in dried form, and its supply depends entirely upon culture weeds. The history of laver culture in Korea about sixty or seventy years is older than in Japan. Significance of laver culture is divided into two aspects, one is food supply in the nation, and the other is export to other countries. Houses engaged in laver culture are about foully thousands, and laver production in 1972 is estimated as 1, 3 bitten sheets. (1 sheet is a dried laver of 20 cm sq, in the shape of paper) Especcially meaning of layer production is the concentration of labour input, and systematic management of labour. From around 1920, the method of laver culture was introduced by Japanese Imperialism for mono culture in shallow seas, and mass products of laver is provided to Japan market, DOMESTIC MARKET Fundamental consume function calculates at below, $D_{(68_71)}$=16354 $Y^{0.471}$ $P^{-1.0662}$ where D is total layer demand, Y income variable, P price variable. It means income elasticity is 476. in the whole country, and price elasticity is 1, 07. But generally income elasticity is higher in urban area than in rural area, as shown at 1, 3 in Seoul city. Expence of laver in house expenditure is mutually correlated with another expence, See Table 12 about the relative function. See Table 14 and 16 about the relation between the gathering and the changes of price in auction, wholesale and retail price support system is for two effects, one of which is constraint of the upper price, the other is rise of the lower price. Before the system control, the equation in three year average calculated as below, $Y_{b}$ =18, 907.7455+15435.9364 t (r=0.89) where the origin t=0 is the November and the units are month. Post the system control, $Y_{p}$ =30, 047.9636+1, 631.1721t (r=0.97) therefore, this system has an effect only on the rise of lower price, Average annual margins of laver products at four market levels according to the consumer spent is below. EXPORTING MARKET Japanese demand function of laver products is, Log D=5, 289+1, 108 Log Y-1, 395 Log P (r=0.987) where D is Japanese laver demand, Y income variable, P price variable. according to which income elasticity is 1. 1 and price elasticity is 1.4. Laver production in 1970 tile highest record till then, is estimated as six billion sheets. But the recent improvement of laver culture techniques, the production of seeds and freezing storage of seeds has been stabilized. Futher new culture farms have been developed by means of break- water fences or by floating culture method. These improvements have been backed up with increased demand of laver products. Import quantity and price of Korean laver products are restrained by three organizations, that is producer, distributor and consumer. This relationship calculated by regression equation shows that import is influenced only producer organization, at the sacrifice of consumer profit. For increase to export of laver products, we urgently require to open foreign trade of laver products for Japanese consumer, .and Japan has political responsibility to solve Korean laver structure. But with long run timeseries, as regards Japanese production and import quantity, importing function shows increasing trend as below, 250 million sheets <3, 947.1674+0.005 $L_{g}$ >) 600 million sheets where $L_{q}$ is relative production quantity of laver in Japan. (unit; 100 thousand sheets) Our Export effort should be put on the highly processed products whithin the restraind quote.ote.

  • PDF

오차드그래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화 (High-frequency Plant Regeneration from Mature Seed-derived Callus Cultures of Orchardgrass)

  • 이상훈;이동기;김진수;이병현
    • Journal of Plant Biotechnology
    • /
    • 제30권4호
    • /
    • pp.341-346
    • /
    • 2003
  • In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92% when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61%. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69%. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

억새(Miscanthus sinensis) 성숙 종자로부터의 캘러스 유도 및 식물체 재분화 체계 확립 (Establishment of Callus Induction and Plant Regeneration System from Mature Seeds of Miscanthus sinensis)

  • 조준형;변지희
    • 한국자원식물학회지
    • /
    • 제24권5호
    • /
    • pp.628-635
    • /
    • 2011
  • 본 연구는 최근 한방자원, 사료자원, 바이오에너지 자원 등 다양하게 이용되는 국내 자생 억새(Miscanthus sinensis)의 대량생산 및 신품종 개발을 위한 조직배양체계 확립을 위해 수행되었다. 이를 위해 억새 완숙종자로부터의 캘러스 유도와 재분화를 위한 식물생장조절제의 적정농도를 규명하였다. 억새의 성숙종자유래 배발생 캘러스 유도를 위해 2,4-D, IBA, NAA를 1~10 $mg{\cdot}L^{-1}$의 농도로 단용 처리한 결과, 5 $mg{\cdot}L^{-1}$ 2,4-D 처리에서 가장 높은 85.3%의 캘러스 유도율과 캘러스의 증식을 보였으며 조직배양 과정 중 갈변화율도 가장 낮았다. 또한, 캘러스의 재분화를 위해 옥신인 NAA와 Kinetin, 2-iP, 또는 BAP 등의 사이토키닌을 혼용 처리한 결과, 각각 19.0%~59.0%, 23.0%~67.3%, 14.7%~83.7%의 재분화율을 보여 NAA와 BAP의 혼용 처리구가 NAA와 Kinetin 또는 2-iP와 혼용 처리구보다 식물체 재분화에 효과적이었다. 특히 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리된 배지에서의 재분화율이 83.7%로 가장 높게 나타났으며, 캘러스 당 재분화 식물체 개수도 5.5개로 동일농도의 2-iP 또는 Kinetin 혼용 처리 시 2.1 및 2.0개보다 많았다. 본 연구결과 억새 성숙 종자로부터의 배발생 캘러스 유도에는 5 $mg{\cdot}L^{-1}$ 2,4-D가 그리고 캘러스의 재분화에는 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리가 가장 효율적이었다. 본 연구를 통해 확립된 조직배양체계는 억새의 대량생산 및 신품종 개발에 유용할 것으로 판단된다.

성인 남성의 기본 상의 원형 비교 연구 - 3차원 가상착의 시스템을 이용하여 - (A Comparative Study on Men's Bodice Slopers - Using a 3D Virtual Garment System -)

  • 홍은희;서미아
    • 복식문화연구
    • /
    • 제20권3호
    • /
    • pp.403-415
    • /
    • 2012
  • This study did a comparative analysis on the patterns of several male bodice slopers, followed by evaluating the appearance through a virtual garment simulation, which used a three-dimensional virtual garment system. Through this process, this study attempted to comparatively analyze the suitability of the garment or each sloper according to body parts. The analysis of the drawing formulas of bodices slopers had the following results. With a standard chest size, all six slopers had a 1:4 ratio for a front and back chest size; 1:5 or 1:6 for a front and back breast size 1:5 or 1:4 for a neck base circumference; and 1:20 or 1:12 for the chest size. When comparing the anthropometric figures and the actual measurements of each body part for the bodice slopers, the actual measurements secured extra amounts in the drawing process. The evaluation results for appearance of the six men's bodice slopers showed that the slopers used by companies (E and F) had top scores followed by the slopers made for (C and D), while the sloper for education (A) received the lowest scores. Regarding the results of the variance analysis verifying the significant difference between the slopers, twenty-five items had significant differences with the exception of four items on the front side and one item on the backside.

BIM을 적용한 목조건축문화재 기록데이터 활용방안에 관한 연구 (A Study on the Utilization of Documentation using BIM on Wooden Architectural Cultural Assets)

  • 신병욱
    • 한국농촌건축학회논문집
    • /
    • 제21권4호
    • /
    • pp.25-36
    • /
    • 2019
  • The purpose of the study is to analyze how to document survey and repair data on wooden architecture culture assets. Documentation was analyzed in comparison to digital developments from the past to the present. Although the accuracy of survey equipment has been improved by digital development, survey drawing has not changed. For example, a 3D Scanner, survey equipment, was introduced but is being used for conversion into 2D data. Data provided by the drawing included in the survey and repair reports were not accumulated. As it stands, it is inaccessible and disorganized. Data generated from the survey, repair, and maintenance has to be consistent. A BIM system was proposed to integrate information on wooden architecture cultural assets.