• 한국초지조사료학회지
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• 제21권2호
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• pp.49-52
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• 2001

conditions for callus induction and plant regeneratin from seeds of lawngrass (Zoysia japonica Steud.) were confirmed in this study. MS (Murashige and Skoog) medium containg 2,4-D 3 or 5mg/l was used for callus induction, and MS medium with different volumes of BA, NAA and kinetin hormones was used to regenerate the plants from induced calli. MS basic medium containing agar with no hormones or kinetin 1.0mg/l and MS basic medium containing gelite and NAA 1.0mg/l were higher for green callus induction. MS medium containing agar and kinetin 1.0mg/l ws highest degree of efficiency for plant regeneration.

• Korean Journal of Geomatics
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• 제1권1호
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• pp.15-20
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• 2001

In this study, several basic data for road design and GIS data were used for selecting the optimized road alignment database system. The cut and fill volumes were compared with existing manual road design method through the analysis and data application in this database system. We solved and estimated objective, economic, environmental and technical problems caused in road construction comparing existing manual method with the road alignment which was selected in GIS automatically. Also, we performed three dimensional simulation with the existing road design program and simulation of virtual reality through Virtual GIS. This study showed the method in selecting the optimized road alignment through the analysis and comparison of the selected road alignment. The goal of this study is comparison and analysis of definite cut and fill volume and environmental problem after the road construction through analyzing and comparing the social, economic, technical and environmental aspect in the road alignments with various statistic data.

• KSBB Journal
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• 제30권6호
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• pp.307-312
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• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• 한국컴퓨터그래픽스학회논문지
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• 제7권2호
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• pp.21-28
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• 2001

최근 과학적 데이터의 증가 결과로, 매우 방대한 크기의 볼륨 데이터들이 점차로 일반화되고 있다. 다양한 텍스춰 기반 볼륨 렌더링 알고리듬들이 제안되었으나, 대부분의 방법들이 하드웨어가 갖고 있는 텍스춰 메모리보다 작은 크기의 볼륨 데이터를 가시화하는데 촛점을 맞추고 있다. 본 논문은 멀티파이프 시스템 구조상에서 매우 큰 정적 데이터와 시간 가변 데이터를 위한 새로운 병렬 볼륨 렌더링 스킴에 대해 설명한다. 이 스킴은 큰 볼륨을 동적으로 작은 크기의 블릭으로 분할하고, 이를 그래픽스 파이프에 적절히 할당함으로써 텍스춰 교체 비용을 최소화 한다. 이 기법은, 수행중에 법선 벡터를 계산하고 OpenGL 컬러 행렬을 이용하여 퐁 쉐이딩 영상을 쉽게 생성할 수 있다. 우리는 SGI Onyx2 시스템을 이용하여 큰 크기의 다양한 데이터에 대해 실험한 결과를 보인다.

• 한국농공학회지
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• 제29권2호
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• pp.53-63
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• 1987

Due to their wide range of application, deterministic comprehensive hydrologic models using digital computers have been developed in all countries of the world and researches are being undertaken for their appropriate applications. The aim of this study has been to demonstrate the practical implementation of a physically based distributed hydrologic model, the USDAHL-74 model and to investigate its ability to simulate the long term estimate of water balance quantities in a Korean mountainous watershed. Application of the model to Dochuk watershed indicates the following results. 1.Since the USDAHL-74 model includes all the major components of the hydrologic cycle in agricultural watersheds, thus is comprehnsive, the model seems to have a wide range of application from the fact that simulation results obtained are not only runoff volumes m various time units but their spatial variation as well as even soil moisture within the watershed. 2.An approximate calibration to determine the parameter values in the model using various data obtained from D0chuk shed shows that the simulation error of yearly runoff volume is only 0.6 % and a correlation coefficient between observed daily runoff volume and simulated one is 0.91 in all calibrated period.3.As a verification test of the model, runoff volumes are simulated using 1986 year data without changing the parameter values determined by 1985 year data. The tests show that the USDAHL-74 model is a flexible tool and that realistic production to simulate the long term estimate of runoff in Korean mountainous watershed could be obtained using only a short period of calibration.4. Despite of the encouraging results, there still remain minor problems concerning the practical application of the model to improve the result of simulations. Some of these are the small descrepancies between observed and simulated daily runoff volume appeared in the vicinity of peaks and the recession of1 the daily hydrographs and the model performance for the frozen ground and melting process in the model. 5. Alough the use of parameter with physical significance and the ability to improve calibrations on the basis of physical reasoning represents advantages in the simulation for ungaged watersheds, further researches are needed to use the USDAHL-74 mode to simulate runoff in ungaged watersheds.

• 한국의학물리학회지:의학물리
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• 제27권4호
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• pp.203-212
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• 2016

Dose differences between three-dimensional (3D) and four-dimensional (4D) doses could be varied according to the geometrical relationship between a planning target volume (PTV) and an organ at risk (OAR). The purpose of this study is to evaluate the correlation between the overlap volume histogram (OVH), which quantitatively shows the geometrical relationship between the PTV and OAR, and the dose differences. 4D computed tomography (4DCT) images were acquired for 10 liver cancer patients. Internal target volume-based treatment planning was performed. A 3D dose was calculated on a reference phase (end-exhalation). A 4D dose was accumulated using deformation vector fields between the reference and other phase images of 4DCT from deformable image registration, and dose differences between the 3D and 4D doses were calculated. An OVH between the PTV and selected OAR (duodenum) was calculated and quantified on the basis of specific overlap volumes that corresponded to 10%, 20%, 30%, 40%, and 50% of the OAR volume overlapped with the expanded PTV. Statistical analysis was performed to verify the correlation with the OVH and dose difference for the OAR. The minimum mean dose difference was 0.50 Gy from case 3, and the maximum mean dose difference was 4.96 Gy from case 2. The calculated range of the correlation coefficients between the OVH and dose difference was from -0.720 to -0.712, and the R-square range for regression analysis was from 0.506 to 0.518 (p-value <0.05). However, when the 10% overlap volume was applied in the six cases that had OVH value ${\leq}2$, the average percent mean dose differences were $34.80{\pm}12.42%$. Cases with quantified OVH values of 2 or more had mean dose differences of $29.16{\pm}11.36%$. In conclusion, no significant statistical correlation was found between the OVH and dose differences. However, it was confirmed that a higher difference between the 3D and 4D doses could occur in cases that have smaller OVH value.

• 한국의학물리학회지:의학물리
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• 제30권4호
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• pp.94-103
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• 2019

Purpose: To evaluate the clinical feasibility of knowledge-based planning (KBP) for volumetric-modulated arc radiotherapy (VMAT) in spine stereotactic body radiotherapy (SBRT). Methods: Forty-eight VMAT plans for spine SBRT was studied. Two planning target volumes (PTVs) were defined for simultaneous integrated boost: PTV for boost (PTV-B: 27 Gy/3fractions) and PTV elective (PTV-E: 24 Gy/3fractions). The expert VMAT plans were manually generated by experienced planners. Twenty-six plans were used to train the KBP model using Varian RapidPlan. With the trained KBP model each KBP plan was automatically generated by an individual with little experience and compared with the expert plan (closed-loop validation). Twenty-two plans that had not been used for KBP model training were also compared with the KBP results (open-loop validation). Results: Although the minimal dose of PTV-B and PTV-E was lower and the maximal dose was higher than those of the expert plan, the difference was no larger than 0.7 Gy. In the closed-loop validation, D_1.2cc, D_0.35cc, and D_mean of the spinal cord was decreased by 0.9 Gy, 0.6 Gy, and 0.9 Gy, respectively, in the KBP plans (P<0.05). In the open-loop validation, only D_mean of the spinal cord was significantly decreased, by 0.5 Gy (P<0.05). Conclusions: The dose coverage and uniformity for PTV was slightly worse in the KBP for spine SBRT while the dose to the spinal cord was reduced, but the differences were small. Thus, inexperienced planners could easily generate a clinically feasible plan for spine SBRT by using KBP.

• 한국의학물리학회지:의학물리
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• 제24권4호
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• pp.284-289
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• 2013

양측성 유방암 환자를 대상으로 부피적 조절회전방사선치료와 세기조절방사선치료의 선량학적 결과를 비교하여 효율적인 치료방법을 제시함을 목적으로 한다. 5명의 환자를 대상으로 8개 빔의 세기조절방사선치료와 12개 빔의 세기조절방사선치료, 1회전 부피적 조절회전방사선치료 계획을 수립하였다. 그 결과를 DVH 분석을 통해 선량학적으로 비교분석하였다. PTV의 평균선량과 $D_{98}$을 보면 12-IMRT가 $51.04{\pm}0.57$ Gy (오른쪽), $50.80{\pm}1.07$ Gy (왼쪽), $42.94{\pm}16.16$ Gy (오른쪽), $42.56{\pm}2.09$ Gy (왼쪽)로 우수한 경향을 보인다. HI와 $CI_{90,95}$에서도 12-IMRT가 우수한 결과를 보였다. 중요장기에서는 세치료계획 모두 우수한 결과를 보였지만 12-IMRT에서 가장 낮은 선량을 보였다. 12-IMRT가 PTV와 OAR의 선량학적측면에서 우수한 결과를 보여주었다.

• Radiation Oncology Journal
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• 제20권1호
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• pp.1-16
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• 2002

Purpose : Three dimensional conformal radiotherapy planning is being used widely for the treatment of patients with brain tumor. However, it takes much time to develop an optimal treatment plan, therefore, it is difficult to apply this technique to all patients. To increase the efficiency of this technique, we need to develop standard radiotherapy plant for each site of the brain. Therefore we developed several 3 dimensional conformal radiotherapy plans (3D plans) for tumors at each site of brain, compared them with each other, and with 2 dimensional radiotherapy plans. Finally model plans for each site of the brain were decide. Materials and Methods : Imaginary tumors, with sizes commonly observed in the clinic, were designed for each site of the brain and drawn on CT images. The planning target volumes (PTVs) were as follows; temporal $tumor-5.7\times8.2\times7.6\;cm$, suprasellar $tumor-3\times4\times4.1\;cm$, thalamic $tumor-3.1\times5.9\times3.7\;cm$, frontoparietal $tumor-5.5\times7\times5.5\;cm$, and occipitoparietal $tumor-5\times5.5\times5\;cm$. Plans using paralled opposed 2 portals and/or 3 portals including fronto-vertex and 2 lateral fields were developed manually as the conventional 2D plans, and 3D noncoplanar conformal plans were developed using beam's eye view and the automatic block drawing tool. Total tumor dose was 54 Gy for a suprasellar tumor, 59.4 Gy and 72 Gy for the other tumors. All dose plans (including 2D plans) were calculated using 3D plan software. Developed plans were compared with each other using dose-volume histograms (DVH), normal tissue complication probabilities (NTCP) and variable dose statistic values (minimum, maximum and mean dose, D5, V83, V85 and V95). Finally a best radiotherapy plan for each site of brain was selected. Results : 1) Temporal tumor; NTCPs and DVHs of the normal tissue of all 3D plans were superior to 2D plans and this trend was more definite when total dose was escalated to 72 Gy (NTCPs of normal brain 2D $plans:27\%,\;8\%\rightarrow\;3D\;plans:1\%,\;1\%$). Various dose statistic values did not show any consistent trend. A 3D plan using 3 noncoplanar portals was selected as a model radiotherapy plan. 2) Suprasellar tumor; NTCPs of all 3D plans and 2D plans did not show significant difference because the total dose of this tumor was only 54 Gy. DVHs of normal brain and brainstem were significantly different for different plans. D5, V85, V95 and mean values showed some consistent trend that was compatible with DVH. All 3D plans were superior to 2D plans even when 3 portals (fronto-vertex and 2 lateral fields) were used for 2D plans. A 3D plan using 7 portals was worse than plans using fewer portals. A 3D plan using 5 noncoplanar portals was selected as a model plan. 3) Thalamic tumor; NTCPs of all 3D plans were lower than the 2D plans when the total dose was elevated to 72 Gy. DVHs of normal tissues showed similar results. V83, V85, V95 showed some consistent differences between plans but not between 3D plans. 3D plans using 5 noncoplanar portals were selected as a model plan. 4) Parietal (fronto- and occipito-) tumors; all NTCPs of the normal brain in 3D plans were lower than in 2D plans. DVH also showed the same results. V83, V85, V95 showed consistent trends with NTCP and DVH. 3D plans using 5 portals for frontoparietal tumor and 6 portals for occipitoparietal tumor were selected as model plans. Conclusion : NTCP and DVH showed reasonable differences between plans and were through to be useful for comparing plans. All 3D plans were superior to 2D plans. Best 3D plans were selected for tumors in each site of brain using NTCP, DVH and finally by the planner's decision.

• Wind and Structures
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• 제11권5호
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• pp.361-376
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• 2008

This paper considers internal pressure fluctuations for a range of building volumes and dominant wall opening areas. The study recognizes that the air flow in and out of the dominant opening in the envelope generates Helmholtz resonance, which can amplify the internal pressure fluctuations compared to the external pressure, at the opening. Numerical methods were used to estimate fluctuating standard deviation and peak (i.e. design) internal pressures from full-scale measured external pressures. The ratios of standard deviation and peak internal pressures to the external pressures at a dominant windward wall opening of area, AW are presented in terms of the non-dimensional opening size to volume parameter, $S^*=(a_s/\bar{U}_h)^2(A_W^{3/2}/V_{Ie})$ where $a_s$ is the speed of sound, $\bar{U}_h$ is the mean wind speed at the top of the building and $V_{Ie}$ is the effective internal volume. The standard deviation of internal pressure exceeds the external pressures at the opening, for $S^*$ greater than about 0.75, showing increasing amplification with increasing $S^*$. The peak internal pressure can be expected to exceed the peak external pressure at the opening by 10% to 50%, for $S^*$ greater than about 5. A dominant leeward wall opening also produces similar fluctuating internal pressure characteristics.